• Microbiology and Biotechnology Letters
• /
• v.47 no.2
• /
• pp.220-233
• /
• 2019

Bacterial lysates have become a common ingredient for natural health care. Lactic acid bacteria (LAB) could serve as potential candidates for lysate production: the lactic acids produced by LAB have been utilized for their moisturizing, antimicrobial, and rejuvenating effects, while other substances provide topical benefits and health effects for the skin. Our study aimed to obtain lysate from a LAB S. macedonicus MBF 10-2 through an optimized fermentation using the Response Surface Methodology. Strain MBF10-2 was cultivated in a 2L fermenter tank in de Man Rogosa and Sharpe (MRS) medium and in plant-based peptone modified MRS, i.e. Soy-peptone and Vegitone. The duration and the medium composition (dextrose and soy peptone or proteose peptone) were adjusted to obtain an optimum production of cell lysate. Central Composite Design was employed for Design Expert 7.0.0 by adjusting 3 factors: dextrose (1%, 1.5%, 2%, 2.5%, 3%), soy or proteose peptone (0.5%, 0.75%, 1%, 1.25% and 1.5%), and duration of fermentation (8, 10, 12 14, and 16 h for MRS-Soy peptone and 15, 17, 19, 21, and 23 h for MRS Vegitone). Bacteriocin-Like Inhibitor Substance activity of lysate and pH were used as indicators. The optimum condition for lysate production using MRS Soy Peptone and Vegitone are as follows: dextrose concentration 2.5%, plant-based peptone 1.25%, while optimum fermentation duration were 11.18 h (MRS Soy Peptone) and 17 h (MRS Vegitone) with a starter concentration of 10% at $OD_{600nm}$ $0.2{\pm}0.05$. However, the standard MRS medium produced better quality lysate compared to MRS plant-based peptones.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.45 no.1
• /
• pp.27-36
• /
• 2019

Soybean (Glycine max), as one of the foods with high plant proteins, contains a large amount of bioactive compounds and known to be effective in cardiovascular disease and obesity as well as in improving skin condition. The purpose of this study was to investigate the anti-photoaging effects of soybean fermented with Lactobacillus Rhamnosus ($Bio-Peptone^{(R)}$) by assessment of cytotoxicity against UVB, collagen synthesis after UVB-irradiation, tyrosinase activity, and melanin synthesis. Results showed that $Bio-Peptone^{(R)}$ protected skin fibroblasts against UVB-induced cytotoxicity and increased type I collagen synthesis. Furthermore, $Bio-Peptone^{(R)}$ significantly inhibited tyrosinase activity and reduced melanin contents. This study suggests that $Bio-Peptone^{(R)}$ has protective effects against UVB-induced skin damage. Thus, it is concluded that $Bio-Peptone^{(R)}$ is able to prevent skin damage against UVB and thus acts as anti-photoaging materials by protecting skin forming wrinkles and skin pigments.

• Journal of Applied Biological Chemistry
• /
• v.53 no.3
• /
• pp.126-131
• /
• 2010

In order to maximize the growth of Bifidobacterium lactis BL 740 and soy isoflavone agycones production, we investigated the optimization of a culture medium containing soy hypocotyls, which are the byproducts of the soy manufacturing process, and soy proteins. The ingredients of the medium containing soy materials (S-medium) were selected by fractional factorial design (FFD) and central composite design (CCD) within a desirable range. The FFD was applied by six factors: glucose, cellobiose, fructooligosaccharide, soy peptone, soy protein, and soy hypocotyl. Soy protein, soy peptone, and soy hypocotyl were found to be significant factors from the result of FFD for both the growth of B. lactis BL 740 and aglycone production. The CCD was then applied with three variables found from FFD at five levels each and the optimum values were determined for the three variables: soy peptone, soy protein, and soy hypocotyl. In the case of the growth of B. lactics BL740, the proposed optimal media contained 12.73 g/L of soy protein, 29.55 g/L of soy peptone, and 130.67 g/L of soy hypocotyl. To produce isoflavone aglycones, optimized media was composed of 2.06 g/L, soy protein, 1.25 g/L of soy peptone, and 60.02 g/L of soy hypocotyl.

• KSBB Journal
• /
• v.6 no.4
• /
• pp.369-377
• /
• 1991

The effects of nitrogen sources(sodium glutamate and peptone) on the production of xanthan gum were investigated. The fermentation using sodium glutamate as a nitrogen source is longer than that of peptone. In the initial nitrogen concentration of 0.4-1.0g/L, Bs was about 2.0 and ${\beta}$s was 1.2. The optimal yields were obtained when the carbon source/nitrogen concentration was 10-16. The fermentation time and product yields in the fermentation medium of mixed nitrogen source [sodium glutamate-N(0.75g/L)+peptone-N(0.25g/L)] were similar to those of peptone.

• KSBB Journal
• /
• v.15 no.2
• /
• pp.195-200
• /
• 2000

Studies were made on the optimization of media to c비tivate Thraustochytrium aureum A TCC 34304 for the enhanc엉d p production of linoleic acid. The medium optimization was made with the artificial sea water medium. Yeast extract, sodium g glutamate, peptone and tryptone were considered as nitrogen source. The effect of $\infty$ncentration of nitrogen source as well a as initial glucose on the production of linoleic acid were investigated to optimize the media. The maximum yield of lipid was 0 0.302 mg/g cell mass when initial glucose $\infty$ncentration was 10 g/L and sodium glutamate was used as nitrogen source, a and the yield of linoleic acid to unit cell mass was also maximum to be 8 % in that case. The highest linoleic acid c concentration was obtained in the initial glucose concentration 30 g/L regardless of the kinds of nitrogen source and the | linoleic acid concentration was 0.208 g/L when peptone was supplemented to be 2 g/L.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.33 no.3
• /
• pp.27-44
• /
• 2020

Objectives : The authors conducted randomized, Double-blinded, and placebo-controlled parallel-group clinical experiment study to evaluate efficacy of fermented soybean(Bio-Peptone)cream in pruritus. Methods : The research had been conducted for 4 months from the date of IRB approval(May 26 in 2017) to Sept 2017. The experiment started by randomly distributing 25 subjects with pruritus into experimental group and control group, respectively. The experimental group applied fermented soybean(Bio-Peptone)cream twice a day, in the morning and evening, for one week on itchy area. The effect of the product was evaluated by comparing the PSS(Patient subjective score), moisture level by measuring skin moisture content(Corneometer) and transepidermal water loss(Tewameter), and the Korean version of Skindex-29(index of quality of life improvement) before applying the cream, after applying the cream for one week and after stop applying the cream for one week. The control group conducted identical experiment with the experimental group, except the control group applied placebo instead of the fermented soybean(Bio-Peptone)cream. Results : Pruritus, criterion of the first validation testing, indicates fermented soybean(Bio-Peptone)cream tend to reduce pruritus compare to placebo, although the result is not statistically noticeable. Significant difference in reduction of prutitus, the second validation test was not discovered in both groups. Corneometer and life quality tend to be improved with soy cream than placebo, but not statistically effective and both groups did not show any difference in terms of Tewameter measurement. Conclusions : The result of clinical experiment didn't prove that the fermented soybean(Bio-Peptone)cream is more effective in reducing pruritus than placebo, statistically. The clinical use of soybean product for pruritus requires further studies to be verified.

• Fisheries and Aquatic Sciences
• /
• v.14 no.3
• /
• pp.205-209
• /
• 2011

Organic pollution causes eutrophication and dystrophication, which occur when excessive amounts of organic matter enters seawater. Eutrophication can contaminate sediment and harm aquaculture. Polychaeta species have been shown to restore eutrophic sediment. In this study, we used peptone to simulate a eutrophic environment and detect the levels at which eutrophication became toxic to the polychaete Perinereis aibuhitensis. Peptone concentrations were 0, 100, 200, and 500 mg/L. The median lethal concentrations were 950.35 mg/L at 48 h, 340.34 mg/L at 72 h, and 120.22 mg/L at 96 h, which are much higher than those of other aquatic species. Polychaeta species are highly tolerant of eutrophication. During the 15-day long-term experiment, sediment loss on ignition, as well as seawater total organic carbon and total nitrogen all decreased significantly (P<0.05). However, $NH_4^+$ concentration increased with time. Perinereis aibuhitensis slowed the increment of $NH_4^+$ but could not prevent its increase. Our results indicate that this polychaete is helpful in the recovery of seawater and sediment from eutrophication.

• Journal of Life Science
• /
• v.22 no.5
• /
• pp.575-581
• /
• 2012

This study was conducted to develop an economical optimum medium composition for the mass production of $Lactobacillus$ $plantarum$ and $Lactobacillus$ $reuteri$, livestock probiotics. Medium ingredient factors were selected on the basis of MRS broth composition, and the 15 ingredient variables were as follows: sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, dipotassium phosphate, manganese chloride, magnesium chloride, tween 80, sodium chloride, sodium acetate, ammonium citrate, sodium sulphate, and ferrous sulphate. The Plackett Burman design, consisting of 20 runs, was employed for the analysis of ingredient effects on cell growth of $L.$ $plantarum$ and $L.$ $reuteri$. As a result, sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, sodium acetate, and ammonium citrate positively influenced the growth of $L.$ $plantarum$. Additionally, yeast extract, soy peptone, $K_2PHO_4$, and tween 80 positively influenced the growth of $L.$ $reuteri$. Positive effects were found from sucrose, yeast extract, and soy peptone in the integrated analysis of the effects of both $L.$ $plantarum$ and $L.$ $reuteri$. Finally, effective medium components for both strains were found as follows: sucrose (20.0 g/l), glucose (5.0 g/l), soy peptone (11.0 g/l), yeast extract (5.0 g/l), $K_2PHO_4$ (0.2 g/l), $CH_3COONa$ (2 g/l), and $MgCl_2$ (0.02 g/l).

• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.418-421
• /
• 2001

The gellan was produced by Pseudomonas elodea ATCC 31461 under aerobic condition. Gellan provides various functionalities such as gelling, suspending, stabilizing, emulsifying and binding properties in aqueous systems. In this study, the effect of glucose concentration and nitrogen sources concentration, bacto peptone and $NH_4NO_3$, on the cell growth and the production of gellan were evaluated. The maximum specific yield (g gellan/g cell) of 2.22 was obtained at 1.0% of glucose. Bacto peptone increased cell growth. And nitrogen limitation was essential for higher production of gellan. The highest cell and gellan production were abtained at 0.5 g/${\ell}$ of bacto peptone without $NH_4NO_3$.

• Microbiology and Biotechnology Letters
• /
• v.6 no.4
• /
• pp.161-165
• /
• 1978

Out of 11 organic acid producing strains isolated from fruits, soil, and air, one strain was selected for the study of the citric acid fermentation using Sakaguchi's medium. The organism was identified as Aspergillus niger. When Asp.niger was shaked at 3$0^{\circ}C$ in a cotton plugged 500 mι Erlenmeyer flask with 100 ml of Sakagnchi's medium containing 10% of glucose (Difco), 0.6% of peptone, and mineral, citric acid were produced at the level of 17 gram per liter in 14 days. The citric acid was also produced at the level of 35 gram per liter after the improvements of Sakaguchi's medium-the adaptation, peptone addition, aeration, methanol addition, and glucose addition.