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Biosynthesis of Sesquiterpene in Hairy Root and Cell Suspension Cultures of Hyoscyamus muticus by Elicitation Using Rhizoctonia solani Extracts (Rhizoctonia solani 추출액 첨가에 의한 Hyoscyamus muticus의 현탁세포배양 및 모상근배양에서 Sesquiterpene 생합성)

  • BACK, Kyoungwhan;SHIN, Dong Hyun;KIM, Kil Ung;De HAAS Cynthia R.;CHAPPELL Joseph;CURTIS Wayne R.
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.5
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    • pp.279-284
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    • 1997
  • The extracellular sesquiterpenoids were accumulated in cell and hairy root cultures of Hyoscyamus muticus by elicitation using extracts of Rhizoctonia solani. The vetispiradiene synthase (VS) which is the first committed step in biosynthetic pathway leading to formation of solavetivone, lubimin, and rishitin from isoprenoid intermediate farnesyl pyrophosphate was induced upon elicitation, whereas no sesquiterpenoids and VS activity were detected in both control cell and hairy root cultures. VS activity increased rapidly and reached its maximum 12 h in both cell and hairy root cultures upon elicitor treatment. VS activities were paralleled with the absolute levels of VS polypeptide(s). Interestingly, the profiles of sesquiterpenoid accumulation in hairy root cultures were different from those in cell cultures. The hairy root culture seemed to fail to metabolize solavetivone further to lubimin.

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Detection of Defects in a Thin Steel Plate Using Ultrasonic Guided Wave (유도초음파를 이용한 박판에서의 결함의 검출에 관한 연구)

  • Jeong, Hee-Don;Shin, Hyeon-Jae;Rose, Joseph L.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.18 no.6
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    • pp.445-454
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    • 1998
  • In order to establish a technical concept for the detection of defects in weldments in thin steel plate, an experimental and theoretical investigation was carried out for artificial defects in a steel plate having a thickness of 2.4mm by using the guided wave technique. In particular the goal was to find the most effective testing parameters paying attention to the relationship between the excitation frequency by a tone burst system and various incident angles. It was found that the test conditions that worked best was for a frequency of 840kHz and an incident angle of 30 or 85 degrees, most of the defects were detected with these conditions. Also, it was clear that a guided wave mode generated under an incident angle of 30 degrees was a symmetric mode, So, and that of 85 degrees corresponded to an antisymmetric mode, Ao. By using the two modes, most of all of the defects could be detected. Furthermore, it was shown that the antisymmetric mode was more sensitive to defects near the surface than the symmetric mode. Theoretical predictions confirmed this sensitivity improvement with Ao for surface defects because of wave structure variation and energy concentration near the surface.

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Mineral-Based Slow Release Fertilizers: A Review

  • Noh, Young Dong;Komarneni, Sridhar;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.1
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    • pp.1-7
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    • 2015
  • Global population is expected to reach nine billion in 2050 and the total demand for food is expected to increase approximately by 60 percent by 2050 as compared to 2005. Therefore, it is important to increase crop production in order to meet the global demand for food. Slow release fertilizers have been developed and designed in order to improve the efficiency of fertilizers. Mineral-based slow release fertilizers are useful because the minerals have a crystalline structure and are environmentally friendly in a soil. This review focuses on slow release fertilizers based on montmorillonite, zeolite, and layered double hydroxide phases as a host for nutrients, especially N. Urea was successfully stabilized in the interlayer space of montmorillonite by the formation of urea-Mg or Ca complex, $[(Urea)_6Mg\;or\;Ca]^{2+}$ protecting its rapid degradation in soils. Naturally occurring zeolites occluded with ammonium nitrate and potassium nitrate by molten salt treatment could be used as slow release fertilizer because the occlusion process increased the capacity of zeolites to store nutrients in addition to exchangeable cations. Additionally, surface-modified zeolites could also be used as slow release fertilizer because the modified surface showed high affinity for anionic nutrients such as nitrate and phosphate. Moreover, there were attempts to develop and use synthetic layered double hydroxide as a carrier of nitrate because it has positively charged layers which electrostatically bond nitrate anions. Kaolin was also tested by combining with a polymer or through the mechanical-chemical process for slow release of nutrients.

An instrumented glove for grasp specification in virtual reality based point-and-direct telerobotics

  • Yun, Myung Hwan;Cannon, David;Freivalds, Andris
    • Journal of the Ergonomics Society of Korea
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    • v.15 no.2
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    • pp.165-176
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    • 1996
  • Hand posture and force, which define aspects of the way an object is grasped, are features of robotic manipulation. A means for specifying these grasping "flavors" has been developed that uses an instrumented glove equipped with joint and force sensors. The new grasp specification system is being used at the Pennsylvania State University (Penn State) in a Virtual Reality based Point-and-Direct (VR-PAD) robotics implementation. In the Computer Integrated Manufacturing (CIM) Laboratory at Penn State, hand posture and force data were collected for manipulating bricks and other items that require varying amounts of force at multiple pressure points. The feasibility of measuring desired grasp characteristics was demonstrated for a modified Cyberglove impregnated with FSR (Force Sensitive Resistor) pressure sensors in the fingertips. A joint/force model relating the parameters of finger articulation and pressure to various lifting tasks was validated for the instrumented "wired" glove. Operators using such a modified glove may ultimately be able to configure robot grasping tasks in environments involving hazardous waste remediation, flexible manufactruing, space operations and other flexible robotics applications. In each case, the VR-PAD approach improved the computational and delay problems of real-time multiple- degree-of-freedom force feedback telemanipulation.

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Creep Mechanisms of Calcium-Silicate-Hydrate: An Overview of Recent Advances and Challenges

  • Ye, Hailong
    • International Journal of Concrete Structures and Materials
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    • v.9 no.4
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    • pp.453-462
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    • 2015
  • A critical review on existing creep theories in calcium-silicate-hydrate (C-S-H) is presented with an emphasis on several fundamental questions (e.g. the roles of water, relative humidity, temperature, atomic ordering of C-S-H). A consensus on the rearrangement of nanostructures of C-S-H as a main consequence of creep, has almost been achieved. However, main disagreement still exists on two basic aspects regarding creep mechanisms: (1) at which site the creep occurs, like at interlayer, intergranular, or regions where C-S-H has a relatively higher solubility; (2) how the structural rearrangement evolutes, like in a manner of interlayer sliding, intra-transfer of water at various scales, recrystallization of gelled-like particles, or dissolution-diffusion-reprecipitation at inter-particle boundary. The further understanding of creep behavior of C-S-H relies heavily on the appropriate characterization of its nanostructure.

Assessing the Nano-Dynamics of the Cell Surface

  • Bae, Chil-Man;Park, Ik-Keun;Butler, Peter J.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.32 no.3
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    • pp.263-268
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    • 2012
  • It is important to know the mechanism of cell membrane fluctuation because it can be readout for the nanomechanical interaction between cytoskeleton and plasma membrane. Traditional techniques, however, have drawbacks such as probe contact with the cell surface, complicate analysis, and limit spatial and temporal resolution. In this study, we developed a new system for non-contact measurement of nano-scale localized-cell surface dynamics using modified-scanning ion-conductance microscopy. With 2 nm resolution, we determined that endothelial cells have local membrane fluctuations of ~20 nm, actin depolymerization causes increase in fluctuation amplitude, and ATP depletion abolishes all membrane fluctuations.

Improvements to the stability of electric field sensors

  • Lee, Dong-Oh;Robert Boston;Dietrich W. Langer;Joel Falk
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.06a
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    • pp.495-496
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    • 1998
  • The measurement of the amplitude and phase of electric fields on high voltage transmission lines is important for several reasons including a) Metering and determination of power flow, b) protective relaying. and c) fault sensing. The work reported here is directed toward a major improvement to optically based, electric-field sensors. This is a signal processing based technique for overcoming the instabilities of conventional, optically-based, electric-field sensors to changes in optical power or temperature.

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Food service industry in the era of COVID-19: trends and research implications

  • Lee, Seoki;Ham, Sunny
    • Nutrition Research and Practice
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    • v.15 no.sup1
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    • pp.22-31
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    • 2021
  • Coronavirus disease 2019 (COVID-19) is a new type of respiratory disease that has been announced as a pandemic. The COVID-19 outbreak has changed the way we live. It has also changed the food service industry. This study aimed to identify trends in the food and food service industry after the COVID-19 outbreak and suggest research themes induced by industry trends. This study investigated the industry and academic information on the food and food service industry and societal trends resulting from the COVID-19 outbreak. The most noticeable changes in the food industry include the explosive increase in home meal replacement, meal-kit consumption, online orders, take-out, and drive-through. The adoption of technologies, including robots and artificial intelligence, has also been noted. Such industry trends are discussed in this paper from a research perspective, including consumer, employee, and organizational strategy perspectives. This study reviews the changes in the food service industry after COVID-19 and the implications that these changes have rendered to academia. The paper concludes with future expectations that would come in the era of COVID-19.

Macrophage Migration Inhibitory Factor (MIF) Interacts with Bim and Inhibits Bim-mediated Apoptosis

  • Liu, Lingfeng;Chen, Jinzhong;Ji, Chaoneng;Zhang, Jiayi;Sun, Junlei;Li, Yao;Xie, Yi;Gu, Shaohua;Mao, Yumin
    • Molecules and Cells
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    • v.26 no.2
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    • pp.193-199
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    • 2008
  • The pro-apoptotic Bcl-2 family member Bim acts as a sensor for apoptotic stimuli and initiates apoptosis through the mitochondrial pathway. To identify novel regulators of Bim, we employed the yeast two-hybrid system and isolated the human gene encoding macrophage migration inhibitory factor (MIF), a ubiquitously expressed proinflammatory mediator that has also been implicated in cell proliferation, the cell cycle and carcinogenesis. The interaction between MIF and Bim was confirmed by both in vitro and in vivo protein interaction assays. Intriguingly, protein complexes between MIF and the three major Bim isoforms (BimEL/BimL/BimS) could be detected in HEK293 and K562 cells, especially in cells undergoing apoptosis. Moreover, exogenous expression of MIF partially inhibited Bim-induced apoptosis in HEK293 cells. SiRNA-mediated knockdown of MIF increased apoptosis in K562 cells exposed to the chemical oxidant diamide. Endogenous MIF may regulate the pro-apoptotic activity of Bim and inhibit the release of cytochrome c from mitochondria.

An instrumented Glove for Grasp specification in virtual reality based point-and-direct telerobotics

  • Yun, Myung-Hwan;Cannon, David;Freivalds, Andris
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 1996.04a
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    • pp.141-146
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    • 1996
  • Hand posture and force, which define aspects of the way an object is grasped, are features of robotics manipulation. A means for specifying these grasping "flavors" has been developed that uses an instrumented glove equipped with joint and force sensors. The new grasp specification system is being used at the Pennsylvania State University (Penn State) in a Virtual Reality based Point-and-Direct(VR-PAD) robotics implementation. In the Computer Integrated Manufacturing (CIM) Laboratory at Penn State, hand posture and force data were collected for manipulating bricks and other items that require varying amounts of force at multiple pressure points. The feasibility of measuring desired grasp characteristics was demonstrated for a modified Cyberglove impregnated with FSR (Force Sensitive Resistor) pressure sensors in the fingertips. A joint/force model relating the parameters of finger articulation and pressure to various lifting tasks was validated for the instrumented "wired" glove. Operators using such a modified glove may ultimately be able to configure robot grasping tasks in environments involving hazardous waste remediation, flexible manufacturing, space operations and other flexible robotics applications. In each case, the VR-PAD approach improved the computational and delay problems of real-time multiple-degree-of-freedom force feedback telemanipulation.ck telemanipulation.

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