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http://dx.doi.org/10.7779/JKSNT.2012.32.3.263

Assessing the Nano-Dynamics of the Cell Surface  

Bae, Chil-Man (Department of Physiology and Biophysics, State University of New York)
Park, Ik-Keun (Mechanical Engineering, Seoul National University of Technology)
Butler, Peter J. (Department of Bioengineering, The Pennsylvania State University)
Publication Information
Journal of the Korean Society for Nondestructive Testing / v.32, no.3, 2012 , pp. 263-268 More about this Journal
Abstract
It is important to know the mechanism of cell membrane fluctuation because it can be readout for the nanomechanical interaction between cytoskeleton and plasma membrane. Traditional techniques, however, have drawbacks such as probe contact with the cell surface, complicate analysis, and limit spatial and temporal resolution. In this study, we developed a new system for non-contact measurement of nano-scale localized-cell surface dynamics using modified-scanning ion-conductance microscopy. With 2 nm resolution, we determined that endothelial cells have local membrane fluctuations of ~20 nm, actin depolymerization causes increase in fluctuation amplitude, and ATP depletion abolishes all membrane fluctuations.
Keywords
Membrane Fluctuation; Scanning Ion Conductance Microscopy; Micropipette; Actin Depolimerization; Cell Surface;
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