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Differential Proteomic Analysis of Secreted Proteins from Cutinase-producing Bacillus sp. SB-007

  • Ban, Yeon-Hee;Jeon, Mi-Ri;Yoon, Ji-Hee;Park, Jae-Min;Um, Hyun-Ju;Kim, Dae-Soon;Jung, Seung-Ki;Kim, Keun-Young;Lee, Jee-Won;Min, Ji-Ho;Kim, Yang-Hoon
    • The Plant Pathology Journal
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    • v.24 no.2
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    • pp.191-201
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    • 2008
  • Bacillus sp. SB-007 was isolated from pea leaves harvested from the southwestern parts of South Korea through screening on a minimal medium containing 0.2% purified cutin for its ability to induce the cutinase production. However, no cutinase was produced when it was grown in a minimal medium containing 0.2% glucose. A proteomic approach was applied to separate and characterize these differentially secreted proteins. The expression level of 83 extracellular proteins of the cutinase-producing Bacillus sp. strain SB-007 incubated in a cutinase-induced medium increased significantly as compared with that cultured in a non cutinase-induced medium containing glucose. The extracellular proteome of Bacillus sp. SB-007 includes proteins from different functional classes, such as enzymes for the degradation of various macromolecules, proteins involved in energy metabolism, sporulation, transport/binding proteins and lipoproteins, stress inducible proteins, several cellular molecule biosynthetic pathways and catabolism, and some proteins with an as yet unknown function. In addition, the two protein spots showed little similarities with the known lipolytic enzymes in the database. These secreted proteome analysis results are expected to be useful in improving the Bacillus strains for the production of industrial cutinases.

Seismic vulnerability of old confined masonry buildings in Osijek, Croatia

  • Hadzima-Nyarko, Marijana;Pavica, Gordana;Lesic, Marija
    • Earthquakes and Structures
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    • v.11 no.4
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    • pp.629-648
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    • 2016
  • This paper deals with 111 buildings built between 1962 and 1987, from various parts of the city of Osijek, for which, through the collection of documentation, a database is created. The aim of this paper is to provide the first steps in assessing seismic risk in Osijek applying method based on vulnerability index. This index uses collected information of parameters of the building: the structural system, the construction year, plan, the height, i.e., the number of stories, the type of foundation, the structural and non-structural elements, the type and the quality of main construction material, the position in the block and built-up area. According to this method defining five damage states, the action is expressed in terms of the macroseismic intensity and the seismic quality of the buildings by means of a vulnerability index. The value of the vulnerability index can be changed depending on the structural systems, quality of construction, etc., by introducing behavior and regional modifiers based on expert judgments. Since there is no available data of damaged buildings under earthquake loading in our country, we will propose behavior modifiers based on values suggested by earlier works and on judgment based on available project documentation of the considered buildings. Depending on the proposed modifiers, the seismic vulnerability of existing buildings in the city of Osijek will be assessed. The resulting vulnerability of the considered residential buildings provides necessary insight for emergency planning and for identification of critical objects vulnerable to seismic loading.

Development of Real Time Smart Structure Monitoring System for Bridge Safety Maintenance using Sensor Network (센서 네트워크 기반 실시간 교량 안전관리를 위한 지능형 구조 건전성 모니터링시스템 개발)

  • Jo, Byung-Wan;Kim, Heon;Lee, Yun-Sung;Kim, Do-Keun
    • The Journal of the Korea Contents Association
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    • v.16 no.2
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    • pp.221-230
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    • 2016
  • As structures' long term performances and users' safety have been highlighted, a new maintenance technique using IT has drawn attention around the globe. Therefore, throughout the paper, by analyzing bridge's static and dynamic data using wireless measuring sensor, a "real time smart bridge monitoring system" has developed. Smart bridge monitoring system is consists of three main parts a sensor that can measure major members' movement, a wireless system that informs the data from the sensor, and the database system that analysis the data. In order to test the performance of the system, five different were placed on the Olympic Bridge, Seoul. The power system of the sensors was replaced by self-sustain solar energy system. In order to validate data from the real time smart bridge monitoring system, the data was collected for a week from both wireless system and the wired system and the two data were compared to see the relevance.

Implementation ANY-PACS Using Web Module and DICOM Converter on the windows platform (윈도우 플랫폼 상에서 Web Module 과 Converter를 이용한 Any-PACS 구현)

  • Yoon, Min-Jong;Lee, Joon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 1999.05a
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    • pp.93-98
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    • 1999
  • In recent years, medical procedures have become more complex, while financial pressures for shortened hospital stays and increased efficiency in patient care have increased. As a result, several shortcomings of present film-based systems for managing medical images have become apparent. Maintaining film space is labor intensive and consumes valuable space. Because only single copies of radiological examinations exist, they are prone to being lost or misplaced, thereby consuming additional valuable time and expense. In this paper, Any-PACS for image archiving, transmission, and viewing offers a solution to these problems. Proposed Any-PACS consists of mainly four parts such as Web Module, Client-Server Module, Internal Module, Acquisition Module. In addition, Any-PACS system includes DICOM Converter that Non-DICOM file format converts standard file format. In Client-Server Module case, Proposed system is combined both SCU(Service Class Use: Client) part and SCP(Service Class provider: Server)part therefore this system provides the high resolution image processing techniques based on windows platform. Because general PACS system is too expensive for Medium and Small hospitals to install and operate the full-PACS. Also, we constructed Web Module for database connection through the WWW.

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Information Retrieval Using Natural Language for Multimedia Information Management Database System (멀티미디어 정보관리 데이터베이스 시스템에서 자연어를 사용한 정보 검색)

  • 이현창;배상현
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.5
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    • pp.1035-1041
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    • 2004
  • Currently users are tend to use multimedia data types in their applications. Main features of multimedia data types are large amount of data compared to conventional data types. In this reason, it's hard to load data into main memory and to search. That is the cause of occur disk input and output frequently, and decrease the system performance. In this paper, we describe to have fast and efficient access to multimedia data using index technique. Index method presented by paper consists of two parts : one is index file part for keywords and the other is posting file part for the list of file names. Of course, we use keyword. But user is not charge of memory for the keywords. Users just use natural language to insert, delete and search data what he or she wants. Internally, System makes keywords from natural language to get access to multimedia data. It provides convenience to users. Using this study to develop one's application for multimedia, one may have a chance for advanced performance of a system and getting a result speedily.

Design and Implementation of Web-Based MINI-PACS using the DICOM (DICOM을 이용한 웹 기반 MINI-PACS 설계 및 구현)

  • 지연상;이성주
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.5
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    • pp.1095-1104
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    • 2000
  • In recent years, medical procedures have become more complex, while financial pressures for shortened hospital stays and increased efficiency in patient care have increased. As a result, several shortcomings of present film-based systems for managing medical images have become apparent. Maintaining film space is labor intensive and consumes valuable space. Because only single copies of radiological examinations exist, they are prone to being lost or misplaced, thereby consuming additional valuable time and expense. In this paper, mini-PACS for image archiving, transmission, and viewing offers a solution to these problems. Proposed mini-PACS consists of mainly four parts such as web module, client-server module, internal module, acquisition module. In addition, mini-PACS system includes DICOM converter that non-DICOM file format converts standard file format. In client-server module case, proposed system is combined both SCU (service class user: client) part and SCP(service class provider: sewer)part therefore this system provides the high resolution image processing techniques based on windows platform. Because general PACS system is too expensive for medium and small hospitals to install and operate the full-PACS. Also, we constructed web module for database connection through the WWW.

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A Study on the Description of Data Elements for the Citation Index of Academic Journals in Korea: with Special Reference to the Journal of the Korean Society for Information Management (국내 학술지 인용색인을 위한 데이터요소의 기술형태 분석: 정보관리학회지를 중심으로)

  • 김태수;남영광;최석두
    • Journal of the Korean Society for information Management
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    • v.16 no.2
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    • pp.183-199
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    • 1999
  • The three key parts of citation index are the Citation Index, the Source Index and the Permuterm Subject Index. To identify the core elements for citation index database system, it analyzed the items that have been cited in references and source items of Journal of the Korean Society for Information Management from vol. 1 no. l(1984) to vol. 15 no. 3(1998). Ten core elements were identified and the description format was specified respectively. The core elements are author, organization that the author is affiliated, title of article, title or journal, volume/numberlpage numbers, year of publication, keyword, language, subject category, statistics of references in article. Also, characteristics of errors in reference citations were analyzed and categorized in the viewpoint of the citation index development of academic journals in Korea.

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System Implementation of Paper Currency Discrimination by Using Integrated Image Features (통합 영상 특징에 의한 지폐 분류 시스템의 구현)

  • Gang, Hyeon-In;Choe, Tae-Wan
    • The KIPS Transactions:PartB
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    • v.9B no.4
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    • pp.471-480
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    • 2002
  • In this paper, we implemented a real-time system improving the performance of the paper currency discrimination by integrating a weighted region of interest matching algorithm with a weighted shape feature matching algorithm of the blocked image. The system classifies the paper currency by comparing a query image with compared images based on the database that contain images of paper currency. Especially, the system has good efficiency at the contaminated, rotated, and translated paper currency. The system hardware consists of three parts as follows : the paper currency image acquired by CIS(contact image sensor) is applied to the pre-processing part with A/D converter and PLD. Finally the pre-processed image data are classified by the main image processing part with a high-speed DSP based on the proposed algorithm.

Implementation of Non-SQL Data Server Framework Applying Web Tier Object Modeling (웹티어 오브젝트 모델링을 통한 non-SQL 데이터 서버 프레임웍 구현)

  • Kwon Ki-Hyeon;Cheon Sang-Ho;Choi Hyung-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.4B
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    • pp.285-290
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    • 2006
  • Various aspects should be taken into account while developing a distributed architecture based on a multi-tier model or an enterprise architecture. Among those, the separation of role between page designer and page developer, defining entity which is used for database connection and transaction processing are very much important. In this paper, we presented DONSL(Data Server of Non SQL query) architecture to solve these problems applying web tier object modelling. This architecture solves the above problems by simplifying tiers coupling and removing DAO(Data Access Object) and entity from programming logic. We concentrate upon these three parts. One is about how to develop the DAO not concerning the entity modification, another is automatic transaction processing technique including SQL generation and the other is how to use the AET/MET(Automated/Manual Execute d Transaction) effectively.

3DImmersion Type Virtual Environment System : Training Interruption-free Live-Line Workers (무정전 활선작업 피교육자를 위한 3차원 몰입형 가상환경 교육시스템의 개발)

  • 정영범;박창현;김기현;장길수
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.53 no.1
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    • pp.22-30
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    • 2004
  • As an information-oriented society comes, many people use PC and depend on database that network server has. However, the online data can be missed when a blackout happens and also a power failure effects on standard of judgment on Power Quality. Thus, it is reason of a trend using interruption-free live-line work when a trouble happens to power system. However, the 83% among the number of people who receive an electric shock experience when a laborer is doing interruption-free live-line works. In interruption-free method, the education and the training problem has been issued. However, we have a few instructors for that training. Furthermore, the trainees have short training period, just 4 weeks. In this paper, to develope the method that has no restriction of a time and place and reduce the wasteful materials, immersion type virtual reality(or environment) technology is used. The users of a 3D immersion type VR training system can interact with the system by doing same action in the real safe environment. Thus, it can be valuable to apply this training system to a dangerous work like as "Interruption-free live-line work exchanging COS(Cut-Out-Switch)". In this program, the user works with a instruction on the window and speaker and can't work other tasks until each part of the task completed. The workers using this system can use their hands and viewpoint movement as he is in a real environment but the trainee can't use all parts and senses of a real body with the current VR technology. Despite of this weak point, when we consider the trends of improvement in electrical devices and communication technology, we can say that 3D graphic VR application has a high potentiality.