• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1310-1315
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• 2019

Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using $Ni^{2+}$ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.

• 디지털융복합연구
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• 제17권8호
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• pp.439-446
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• 2019

본 연구의 목적은 승마 체험 참여자들의 참여정도에 따른 사회성발달 및 생활만족과의 관계를 분석하는데 있다. 본 연구의 목적 달성을 위해 승마프로그램 참여자들을 모집단으로 설정하고 유층 집락 무선표집법(Stratified cluster random sampling)의 방법으로 표본을 추출하였다. 입력된 자료는 Windows용 SPSS/AMOS 22.0 Version 통계프로그램을 이용하여 연구의 목적에 따라 통계 처리하였고, 그 결과 도출된 결과는 다음과 같다. 첫째, 인구통계학적 특성변인에 따른 승마 체험의 참여정도, 사회성발달과 함께 생활만족은 부분적으로 차이가 있었다. 둘째, 승마 체험 참가자들의 참여정도와 사회성발달은 관계가 부분적으로 있었다. 특히 승마 체험 참여정도가 사회성 발달의 활동성에 관계가 있는 것으로 나타났다. 셋째, 승마 체험 참가정도는 참가자의 생활만족과의 관계에 영향 없는 것으로 나타났다. 넷째, 승마 체험 참여에 따른 사회성발달은 생활만족에 관계가 없는 것으로 나타났다. 다섯째, 승마 체험 참여와 사회성발달 및 생활만족은 인과 관계는 없는 것으로 나타났다.

• 한국지반환경공학회 논문집
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• 제20권1호
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• pp.19-25
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• 2019

본 연구에서는 무선 센서 네트워크 시스템을 이용한 저수지 댐 시설물 전체의 모니터링 시스템의 적용성을 평가하고자 국내의 저수지 댐을 대표할 수 있는 구간을 시범구축 현장으로 선정해, 계측과 동시에 시설물의 상태를 평가할 수 있는 시스템을 구축, 운영하였다. 무선 센서 네트워크 구축을 위해 시설물의 현재 상태를 평가하여 위험인자를 도출함과 동시에 극한상태에서의 위험인자의 한계값을 도출하였다. 위험구역, 위험인자에 적합한 측정항목을 설정하여 현장에서 측정할 센서의 종류 그리고 위치를 결정하였다. 국내의 저수지 댐을 대표할 수 있는 필댐을 대상으로 현장에서 무선 센서 네트워크 시스템을 구축하여 모니터링 함으로써 시스템의 적용성을 평가하였으며, 현재 댐의 계측관리 기술인 부분 집중방식의 계측과 직접 비교함으로써 시스템의 적용성을 검증하였다.

• Journal of Korea Trade
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• 제25권5호
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• pp.19-38
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• 2021

Purpose - This study aims to verify the direct causal relationship between cost competitiveness and global network competitiveness, which are the tangible service quality factors determined by the shipping company, which in turn affect the shipper's customer satisfaction. Additionally, we empirically investigate the intangible, related service qualities determined by shipping companies, such as operational competitiveness and customer relationship quality, and how these then positively affect customer satisfaction through the formation of trust. Therefore, we examine the mediating effect of trust formation among different contractors for shipping services. Design/methodology - In order to examine the shipping company's tangible and intangible service-qualities perceived by the shipper on customer satisfaction and the process of trust formation between contractors, we collected valid data from 114 respondents out of 200 distributed questionnaires. The respondents consisted of domestic freight forwarders who engage with domestic and international shipping and logistics agencies. Descriptive statistics, confirmatory factor analysis, reliability, convergent and discriminant validities, common method bias, and PLS-SEM (partial least square-structural equation model) were analyzed using the program STATA 16. Findings - The findings of this study are as follows. First, our results showed that all hypotheses assumed in this study had statistically significant supporting evidence. Second, it was found that the mediating effect of trust was significant in affecting the quality of intangible service- qualities for customer satisfaction. Third, through supplementary analysis, we found that the global network competitiveness of domestic shipping companies will increase in importance in the future. In conclusion, the theoretical and practical implications of these findings are presented. Originality/value - This study reaffirmed the traditional causal relationship between customer satisfaction and tangible service quality. Additionally, we also contribute to the literature on the understanding of the causal relationship between trust formation and customer satisfaction through intangible interactions from a long-term perspective.

• 한국BIM학회 논문집
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• 제9권4호
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• pp.51-61
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• 2019

In the general manufacturing sector, prototyping used to reduce the risks that can arise with new conceptual products. However, in AEC area, it does not mass-produce a building, so the prototype itself becomes a building. Therefore, it is challenging to have prototyping of the same scale as the real thing, and the prototyping process in architecture is very inefficient. The prototyping process in the design stage typically assumes making a scaled model, partial model, or digital model. However, it is difficult for these models to correspond to the actual building and the environment of time and space such as scale, material, environment, load, physical properties and deformation, corrosion, etc., unlike the actual building. When using the digital twin concept in the prototyping process, it is possible to measure performance from the design stage to the operation stage. The digital twin was found by a method for monitoring based on physical twins and real-time linkage in the operation stage. Therefore, if the digital twin concept is applied at the design stage, it is possible to predict performance using not only current performance but also history information using real-time information. In order to apply the digital twin concept to the prototyping design process, we analyze the theoretical considerations and the prototyping design process of the digital twin, analyze the cases and research results where the prototyping design was applied, Provide an applied prototyping design process. The proposed process is tested through a pilot project and analyzed for potential use.

• 한국학교ㆍ지역보건교육학회지
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• 제22권3호
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• pp.29-39
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• 2021

Purpose: This study aims to investigate the mediating effect of grit in the relationship between body image and health promotion lifestyle for nursing students. It was implemented to present basic data for the development of educational programs to establish the health promotion lifestyle of nursing students. Methods: The participants of this study were 169 nursing students in three universities in G City, J Province. Data were descriptive statistics, t-test, ANOVA, Pearson correlation analysis. Also mediation analysis was performed according to the Baron and Kenny method and Sobel test. Results: Health promotion lifestyle is positively correlated with body image(r=.53, p<.001), grit (r=.37, p<.001), and body image is positively correlated with grit(r=.33, p<.001). The grit showed a partial mediating effect between the body image and the health promotion lifestyle(Z=3.21, p<.001), the positive the body image(β=.46, p<.001), the higher the grit(β=.22, p<.001), the healthier the health. The level of health promotion lifestyle increased, and the explanatory power to explain the health promotion lifestyle was 33%. Conclusion: Therefore, in order to improve the health promoting lifestyle level of nursing students, it is important to change their perception so that they can view their body image positively. In addition, it suggests that nursing students need to cultivate grit so that they can continuously pay attention to and strive for their health promotion lifestyle.

• 한국구조물진단유지관리공학회 논문집
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• 제26권5호
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• pp.143-150
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• 2022

이 논문은 내진 성능 향상 목적으로 외부 강재로 보강한 콘크리트 부재의 휨 성능에 대한 실험적 연구이다. 구조물을 보강하기 위하여 벽과 기둥을 보강재로 감싸는 형식의 보강 방법을 적용하였는데, 이 구조물에서 가장 긴 지간을 갖는 벽체 일부 단면을 실물 크기로 제작하여 정적 재하 실험을 수행하는 방법으로 강성 보강효과를 확인하였다. 실험 결과, 보강 단면은 충분한 보강 성능을 발현하는 것을 확인하였으나, 가력기의 용량 부족과 안전상의 문제로 파괴까지의 거동을 확인하지는 못하였다. 보강재가 벽체에 작용하는 면외방향 모멘트를 효과적으로 부담하고 있는 것을 확인하였고, 측정된 변위와 철근 변형률 등의 분석을 통하여 보강 전 부재에 비하여 부재 강성과 휨모멘트 저항능력이 향상되었음을 검증하였다.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제28권3호
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• pp.7-16
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• 2022

Purpose: This study aims to establish the basic directions of the modular airborne infection isolation(AII) ward. Considering a specific function and purposed use as a modular AII ward, it is a chance to derive an address of current modular technology by overview the limitation and improvement of the existing modular architecture. Methods: In addition to the literature analysis on the configuration system of mobile hospitals, research cases on the operational effectiveness of the domestic and foreign mobile construction systems are analyzed. Results: In order to meet the various and strict space guidelines of the AII ward and a chance to improve limitations of uniformed existing modulars, AII modular the negative pressurized care setting should be minimized a structural restriction for reflecting its system on a architectural plan. For this unique requirements, it could be possible to apply various space boxes called infill box which needs to secure a large-scale space. So, a rahmen structure system could be adaptable for this purpose. A dead space between beams of the rahmen structure is to be used for MEP installation. Partial separation, dismantling, and repair should be possible by separating the MEP and infill box from the structure. The infill box must keep 3.5m width under the current Road Traffic Act. Implications: It is necessary to utilize and develop an improved construction method that can reduce the problems of existing steel modular and PC modular.

• 국제학술발표논문집
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• The 3th International Conference on Construction Engineering and Project Management
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• pp.1346-1351
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• 2009

In this paper we propose a framework for an Object Relational IFC Server (OR-IFC Server). Enormous amounts of information are generated in each project. Today, many BIM systems are developed by various CAD software vendors. Industry Foundation Classes (IFC) developed by International Alliance for Interoperability (IAI) is an open standard data model for exchanging data between the various BIM tools. The IFC provides a foundation for exchanging and sharing of information directly between software applications and define a shared building project model. The IFC model server is a database management system that can keep track of transactions, modifications, and deletions. It plays a role as an information hub for storing and sharing information between various parties involved in construction projects. Users can communicate with each other via the internet and utilize functions implemented in the model server such as partial data import/export, file merge, version control, etc. IFC model servers using relational database systems have been developed. However, they suffered from slow performance and long transaction time due to a complex mapping process between the IFC structure and a relational-database structure because the IFC model schema is defined in the EXPRESS language which is object-favored language. In order to simplify the mapping process, we developed a set of rules to map the IFC model to an object-relational database (ORDB). Once the database has been configured, only those pieces of information that are required for a specific information-exchange scenario are extracted using the pre-defined information delivery manual (IDM). Therefore, file sizes will be reduced when exchanging data, meaning that files can now be effectively exchanged and shared. In this study, the framework of the IFC server using ORDB and IDM and the method to develop it will be examined.

• 한국시뮬레이션학회논문지
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• 제19권4호
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• pp.111-122
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• 2010

본 연구에서는 여러 제조 공정에서 발생하는 주기 신호의 불규칙한 길이를 보정하기 위하여 시불변 특징점 추출 및 정합(Time Invariant Feature point Extraction and Matching, 이하 TIFEM)을 이용한 길이보정 알고리즘을 제안한다. 신호 중간에 길이 변동이 발생 하는 주기신호의 경우 정확하게 길이를 보정하기 위해서는 더 많은 수의 특징점이 필요하며, 추출된 특징점은 신호의 패턴 정보를 포함하고 시간과 크기에 불변한 성질을 가져야 한다. 본 연구에서 제안하는 TIFEM알고리즘은 위의 성질을 가지는 신호 고유의 특성을 추출하고 추출한 특성들을 각각 시점에 해당하는 특성 벡터로 구성한다. 구성된 특성 벡터에서 유효한 벡터만을 걸러내어 길이보정을 위한 특징점으로 선정한다. 선정된 특징점들을 정합한 후 구간별로 길이를 보정하여 보다 정확한 주기 신호의 길이보정을 수행한다. 제안한 알고리즘의 성능을 검증하기 위하여 실제 반도체 공정에서 발생되는 3종류의 신호를 모방하여 생성한 실험데이터를 이용하여 실험을 수행하였다.