• 대한수의학회지
• /
• 제57권3호
• /
• pp.155-158
• /
• 2017

Surgical castration performed to reduce male-associated problems results in pain and microbial infections in male animals. Therefore, immunocontraception, which is mediated by the animal's own antibodies against reproductive hormones, has been recommended as an alternative to surgical castration when considering the animal's welfare. In this study, a new immunocontraceptive vaccine composed of six tandem copies of gonadotropin-releasing hormone (GnRH) fused to rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was developed, and its efficacy was evaluated in male rats. Three different doses (10, 50, and $100{\mu}g$) of recombinant GM-CSF-GnRH protein were injected three times at intervals of two weeks into male rats. The rats vaccinated with three doses of GM-CSF-GnRH produced a significantly higher level of antibodies against GnRH than that in the negative control rats. Severe atrophy of gonads was observed in rats vaccinated with three doses of GM-CSF-GnRH but not in the negative control rats. The results reveal that the new GnRH vaccine conjugated with rat GM-CSF induces efficient immunocontraception in male rats. This formulation of the immunocontraceptive vaccine would be applicable to both domestic and pet male animals.

• 동의생리병리학회지
• /
• 제35권4호
• /
• pp.117-123
• /
• 2021

Gefitinib, a first generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI), provides obvious clinical benefit in patients with EGFR-mutant non-small cell lung cancer (NSCLC). However, patients ultimately develop gefitinib resistance which mainly caused by EGFR T790M secondary mutation. In the current study, we investigated whether the root extract of Scutellaria baicalensis (SB) overcomes gefitinib resistance. Gefitinib-resistant H1975 human NSCLC cells (EGFR L858R/T790M double mutant) were treated with gefitinib and/or ethanol extract of SB (ESB) to evaluate the effect of ESB on the gefitinib sensitivity. The cell viability was measured by MTT assay and trypan blue exclusion assay. The colony-forming ability was evaluated by anchorage-dependent colony formation assay. Combined treatment with gefitinib and ESB markedly decreased the cell viability and colony formation than single treatment with gefitinib or ESB in H1975 cells. In addition, cells treated with both gefitinib and ESB exhibited a significant increase of sub-G1 DNA content which indicates apoptotic cells compared with those treated with gefitinib or ESB alone. As a molecular mechanism, combined treatment with gefitinib and ESB strongly downregulated the phosphorylation of ERK and JNK than single treatment with gefitinib or ESB. Taken together, our results demonstrate that ESB sensitizes H1975 cells to gefitinib treatment. We cautiously propose that ESB can be used in combination with gefitinib for the advanced NSCLC patients with acquired resistance to EGFR TKIs.

• 대한전자공학회논문지SD
• /
• 제45권4호
• /
• pp.117-123
• /
• 2008

본 논문에서는 규칙적인 형태의 NoC 중 mesh 구조를 기반으로 한 어플리케이션 코어 매핑 알고리즘 연구 내용을 소개한다. 제안된 알고리즘은 ant colony optimization(ACO) 기법을 이용하여 주어진 SoC 내장 코어 및 NoC 특성 정보를 대상으로 가장 효과적인 코어 배치 결과를 도출한다. 설계 목적으로 사용된 네트워크 지연 시간 측정을 위해 평균 흡수 계산 결과를 이용하였으며 제한 조건으로는 NoC 대역폭을 기준으로 하였다. 12개의 코어로 구성되는 실제 기능 블럭을 대상으로 실험한 결과 계산 시간이나 매핑 결과 모두 우수함을 확인할 수 있었다.

• 대한전자공학회논문지SD
• /
• 제45권1호
• /
• pp.43-49
• /
• 2008

본 논문에서는 Ant Colony Optimization(ACO)을 이용하여 Single-Error Correcting & Double-Error Detecting(SEC-DED)을 제공하는 메모리 ECC 체커 회로의 소비전력을 절감하는 방안을 제시한다. H-매트릭스를 통해 구현되는 SEC-DED 코드인 Hsiao 코드의 대칭성과 H-매트릭스 구성상의 높은 자유도를 이용하여 회로의 면적, 딜레이에 영향을 주지 않고 최소의 비트 트랜지션이 일어나도록 H-매트릭스를 최적화한다. 실험을 통하여 H-매트릭스의 최적화를 위한 ACO 매핑과 파라메터의 설정을 알아보고 이의 구현 결과를 랜덤 매트릭스 구성을 통한 방식 및 기존의 GA알고리즘을 이용한 최적화 방식과 비교하여 소비 전력이 기존의 방식에 비해 절감될 수 있음을 보여준다.

• 대한미생물학회지
• /
• 제21권2호
• /
• pp.271-276
• /
• 1986

Amphotericin B and rifampin or 5-fluorocytosine were tested for synergism against Candida albicans in a synthetic mdium. The test was based on viability studies in which fungicidal activity was determined during the incubation hours of drug exposure. Concentration of amphotericin B(0.2ug per ml and 0.4ug per ml) in combination with inactive concentration of rifampin(12.5ug per ml) or 5-fluorocytosine(25ug per ml) resulted in rapid decrease of colony froming unit(CFU). On the basis of these and earlier studies, it is concluded that amphotericin Band rifampin or 5-fluorocytosine are synergistic against various yeasts and yeast-like fungi under widely differing experimental conditions.

• 산업공학
• /
• 제17권spc호
• /
• pp.103-110
• /
• 2004

This paper presents a two-phase method for the vehicle routing problems with time windows(VRPTW). In a supply chain management(SCM) environment, timely distribution is very important problem faced by most industries. The VRPTW is associated with SCM for each customer to be constrained the time of service. In the VRPTW, the objective is to design the least total travel time routes for a fleet of identical capacitated vehicles to service geographically scattered customers with pre-specified service time windows. The proposed approach is based on ant colony optimization(ACO) and improvement heuristic. In the first phase, an insertion based ACO is introduced for the route construction and its solutions is improved by an iterative random local search in the second phase. Experimental results show that the proposed two-phase method obtains very good solutions with respect to total travel time minimization.

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
• /
• pp.120-122
• /
• 2003

It was investigated whether developmental characteristic of foundation queens of Bombus ignitus collected in the 11 localities including Jeongsun have any effects by the first oviposition days. Materials and Methods: materials - insect : foundation queens of Bombus ignitus collected in the 11 localities. Methods - we investigated the rate of colony foundation, progeny-queen production and the number of adults emergence for the relationship between first oviposition day and colony development of B. ignitus (omitted)

• 도서관문화
• /
• 제17권2호
• /
• pp.3-8
• /
• 1976

We sampled the fungi on the grounds of the floor and books in the library for the purpose of attack the fungi in the traditional library. We cultured it on the Sabouraud Dextrose Agar(Baltimore Biological Laboratory) and was obtained the fungi of six species, Here irradiated the ultra violet lamp(100V, 15W, 253Å), which was occurred to the colony after 24 hours. And irradiation by 2hrs/day for 2days here. The other method, we were sterilized test by the methanal fumigation The following results were obtained 1. All rearing is suspensed the result of the ultra violet rays irradiation, but the colony that is gained from the grounds of the floor in the library is not sterilized. 2. The result of air tight box to methanal 14ℊ/㎥ for the 24hours fumigation, each test group(the fungi of six species) is all sterilized.

• 미생물학회지
• /
• 제22권3호
• /
• pp.143-150
• /
• 1984

The effect of growth inhibitors on colonial growth of cellulolytic fungus, genus Trichoderma, was investigated to develop a method for the effective screening of various auxotrophs and hypercellulolytic strains. As the results, it was revealed that non-ionic detergents such as Sodium deoxycholate and Triton X-100 were the better ones as a growth inhibitor than Oxgall which has been used to restrict the colony size in genus Trichoderma. Each individual colony remained distinct on minimal plate supplemented with 0.05% Triton X-100 for as long as two or more weeks. The screening of 150 to 200 colonies simultaneous on a single plate was possible in the presence of Triton X-100. The effect of Triton X-100 on simultaneous screening was higher than that of Oxgall by a factor of two.

• 식물병연구
• /
• 제22권4호
• /
• pp.269-275
• /
• 2016

E. pyrifoliae에 의한 과수 가지검은마름병은 국내에서 1995년 최초 발생이래 2016년까지 꾸준히 발생하여 과수농가에 피해를 주고 있는 세균병해이다. 가지검은마름병 발생 농가의 폐원조치 및 공적방제로 인한 경제적 피해 감소를 위하여, 병징이 관찰되는 이병조직 및 건전조직 내의 병원 세균을 검출하여 효율적 관리방안을 마련하고자 본 연구를 수행하였다. 가지검은마름병원세균의 검출은 genomic DNA 추출과정을 생략한 순수 균총만을 이용하는 colony-PCR을 이용하였으며, 이를 위해 ERIC 지역에서 제작된 가지검은마름병원세균 특이 프라이머 EpSPF/EpSPR 프라이머쌍을 선발하였다. 특이 프라이머를 활용한 colony-PCR 방법으로 2014-2015년 4-10월까지 사과나무 생육기간 동안 가지검은마름병 발생상황을 모니터링한 결과, $25^{\circ}C$ 일 평균 온도 기간인 5월 중순부터 7월 초순까지 발병이 가장 빈번하였다. 발병가지 내 병원세균의 존재유무 검정 결과 병징 부위와 이로부터 20 cm 내 건전조직에서만 병원세균이 지속적으로 검출되었다. 따라서 이미 발생한 가지검은마름병을 효율적으로 관리하기 위해 이병조직과 건전조직 경계 부위로부터 20 cm 이상에서 가지치기를 하는 것이 매우 적절할 것으로 판단된다.