미생물학회지 (Korean Journal of Microbiology)

  • 제22권3호
  • /
  • Pages.143-150
  • /
  • 1984
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

섬유소분해균인 Trichoderma의 群體生長의 유도에 관하여

Induction of Colonial Growth of the Cellulolytic Fungus, Genus Trichoderma

  • 박희문 (서울大學校 自然科學大學 微生物學科) ;
  • 홍순우 (서울大學校 自然科學大學 微生物學科) ;
  • 하영칠 (서울大學校 自然科學大學 微生物學科)
  • Park, Hee-Moon (Dep. of Microbiology, College of Natural Science, Seoul Natural University) ;
  • Hong, Soon-Woo (Dep. of Microbiology, College of Natural Science, Seoul Natural University) ;
  • Hah, Yung-Chil (Dep. of Microbiology, College of Natural Science, Seoul Natural University)
  • 발행 : 1984.01.01
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초록

The effect of growth inhibitors on colonial growth of cellulolytic fungus, genus Trichoderma, was investigated to develop a method for the effective screening of various auxotrophs and hypercellulolytic strains. As the results, it was revealed that non-ionic detergents such as Sodium deoxycholate and Triton X-100 were the better ones as a growth inhibitor than Oxgall which has been used to restrict the colony size in genus Trichoderma. Each individual colony remained distinct on minimal plate supplemented with 0.05% Triton X-100 for as long as two or more weeks. The screening of 150 to 200 colonies simultaneous on a single plate was possible in the presence of Triton X-100. The effect of Triton X-100 on simultaneous screening was higher than that of Oxgall by a factor of two.

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