• 대한환경공학회지
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• 제29권6호
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• pp.684-688
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• 2007

입자상 알루미늄 충전 복극전해조를 이용하여 불소함유 수용액을 정전위 전해한 결과를 아래에 요약하였다. 이온크로마토그래피로 전해 시료를 분석한 결과 인가전압 1, 5, 10 V에서 불소이온 제거율은 각각 53, 73, 90%이었다. 지지전해질농도를 10, 30, 50, 70 mg/L로 조절하고 알루미늄 충전율을 0, 25, 50, 75%로 충전 후 전기량을 측정한 결과 지지전해질 농도가 50 mg/L일 때와 충전율이 75%일 때에 전기량이 $2.58A{\cdot}hr$이었다. 불소농도를 30, 50, 70 mg/L로 하여 10 V에서 3시간 전해실험 한 결과 불소제거율이 각각 93.3, 80.0, 68.6%이었고 전기량은 2.59, 3.89, $5.43A{\cdot}hr$이었다. 또한 단위전기량 당 불소 제거량은 불소농도 30, 50, 70 mg/L 에서 4.0, 3.5, $2.0mg/A{\cdot}hr$이었다.

• 식물조직배양학회지
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• 제21권5호
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• pp.309-314
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• 1994

간편하면서도 효율적인 단자엽 식물의 형질전환 기법을 개발하기 위하여 배발생 세포를 직접 electroporation하여 DNA를 도입하는 실험을 벼와 잔디에서 실시하였다. 잔디는 수정 후 3주된 미숙배에서 캘러스를 유도하였으며, 2.4-D가 1 mg/L 함유된 액체 MS배지로 옮겨 진탕배양한 것을 electroporation 실험에 이용하였다. 벼는 20 mm 정도의 미숙화서 유래의 캘러스를 액체 N$_{6}$배지(1 mg/L 2.4-D 함유)에서 진탕배양하여 획득한 세포주를 사용하였다. 액체 진탕배양한 세포괴를 GUS expression vector인 pGA1074 (30 $\mu\textrm{g}$/ml)와 함께 MS 액체 배지에서 Electroporation하였다. 세포벽과 세포막을 통한 세포로의 DNA 전이는 GUS 유전자의 발현 여부 및 정도에 따라 결정하였다. 400 volt, 800 $\mu$F capacitance로 electroporation 처리된 벼와 잔디의 세포괴들은 200 ${\mu}\ell$ (packed cell volume)의 세포괴 당 25 unit (1 unit=파란색을 띤 독립된 세포군) 이상의 빈도로 GUS 활성을 나타내었다. 반면에 무처리 세포주 및 처리한 비배발생 세포주에서는 GUS 발현이 일어나지 않음을 반복적으로 확인차였다. 따라서 electroporation에 의한 벼와 잔디의 형질전환실험에서 원형질체 대신 intact한 배발생 세포가 이용될 수 있음을 의미한다

• Journal of Microbiology and Biotechnology
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• 제31권3호
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• pp.349-357
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• 2021

Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.

• 한국자원식물학회지
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• 제6권1호
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• pp.25-32
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• 1993

Many plants, which collected from Korea, were applied to antitumor and cytotoxic screeing tests against sarcom 180 a ascitec in mice, V-79 KB and P388 cultured cells. The results are summarixed as follows:1) The total packed cell volum method has been used for the antineoplastic screening for from natural higher plants in Korea. By this method, we have found out that the root, leaf and stem of Tripterygiu, regelii Spragne & Taketa having strong antineoplastic activity and also Rumex Japonicus Houtt. Eragrositis ferru-ginea Beauv. and Patrinia scabio-saefolia Fischer showed significant activity to anticancer tumor while cynanchum wilfordii Hemsley, and Rosa polyantha Sieb. et Zacc. showed slight activity to antitumor. 2) Among the 13 tested plants, the root and stem of Tripterygium regelii Spragne & Taketa and Amethystanthus excisus Nakai showed strong antitumor activity by the V79 cytotoxic cell screening test. 3) Twelve plants, which are glowing in mountainous area of Korea tested to anticancer activity. From the results, Eragrositis ferru-ginea Beauv., Angelica gigas Nakai, Geranium sibiricum L., Patrinia scabio-saefolia Fisher, Cynanchum wilfordii Hemsley, and Rubia akane Nakai have been proved to be anti-cancer plants by using P388 cell cultured method. 4) Tripterygiu, resgelii Spragne & Taketa, Eragrositis ferru-ginea Beauv., Patrinia scabio-saefoli Fisher, Cynanchum wilfordii Hemsley and Rasa polyantha Sieb. et Zacc., var. genuina Thunb. showed strong anti-tumor activity both total packed cell volume method and Cytotoxicity method.

• 대한수의학회지
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• 제46권3호
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• pp.249-254
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• 2006

To determine the effects of exposure to formalin on the secondary stress indices, changes in haematology and blood chemistry were monitored in healthy crucian carps (Carassius auratus). Fishes were separately exposed in a concentration range of 125 to 500 ppm formalin for 60 min. After exposure, red blood cell (RBC) count and packed cell volume (PCV) were elevated in the 500 ppm formalin exposed group. However, mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were decreased significantly in the 500 ppm formalin exposed group. Total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, total bilirubin, inorganic phosphorus (IP) and magnesium were significantly increased at a concentration of 500 ppm. Alkaline phosphatase (ALP) and glucose were increased at a concentration of 500 ppm, but this was not significant. Lactate dehydrogenase (LDH) and calcium were significantly decreased at concentrations of 250 and 500 ppm. AST, ALT, glucose and magnesium were significantly increased in the 250 ppm formalin exposed group. These results suggests that formalin exposure might cause some damage in the liver and kidney of crucian carp.

• 한국자원식물학회지
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• 제5권2호
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• pp.85-93
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• 1992

This experiment was Conducted ta screening for the anti-cancer efficacy from the wild plants which are naturally growing in the Korea. The results are as follows. The results were shown thatZea may L. nad significantly effects on mediculal efficacy anganist anti-tumor by usulg the totalpacked cell volume methods and also, severals plants, such as Sofonum nigrum, Patrinia hispidoBunge, Eragrostis, ferrugenia Beauv, Salaginela pouzolgiana Spring, Platrycarya strobilacea Bunge,Codonopsis lanceolata Benth. et Hook fil. which are collected from Giri and Mooju mountain in Koreaand Nagano in Japan were showed effects on auti-tumor. But the pharmaceologial activities ofPharbitis nil Choisy was believed to strong effec on anti - cancer tumors, while toxicity of its wasshown high that induced te kill all used mice. Extraction of Patrinia hispida Bunge, Pharbitis nilChoisy, Toilis japonica DC, Eragrostis erruginea Beauv. and Forsythia koreana Nakai showed effec-tively supressed on growth rate of cancer tumor by the below 50 percent of T/C ratio at 30mg /mlof extraction from plant. That Is strong activity while Reynouxria japonica Houtt. was observed onlymild activities. The above results many possibly suggest that Patrinia hispido Bunge and Eragrostisferrugina Beauv. inhibited the growth of cancer tumor by the both total packed cell volume methodand cytotoxicity method. Although basic research is still going on, we will find out an accurate moth-od for developing useful medicinal plant to improve pharmacological activites against anti-cancertumor, especialy, in Eragrostis ferruginea Beauv.

• 한국자원식물학회지
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• 제5권1호
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• pp.11-23
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• 1992

The experiments tvere carried out to different temperature which affected growth, yield, proximate and antitumor activity in the Codonopsis lanceolata. Growth of aerial part and subterranean partwere best at 2$0^{\circ}C$ and lower 3$0^{\circ}C$. Components of fat, protein and fiber were best at 3$0^{\circ}C$. Wild C.fonceoforo had higher contents of fat, protein, fiber and ash as compared with cultur'ed C.lanceolataArginin was predominant amino acid in both wild and cultivated C.laceolata No significant differ-ence in the mineral contents was found between the wild root and the cultivated at 30'c inbiotronroom. No minerals difference in the contents was found between the cultivated temperature.The content of elements of inorganic metal differs according to the part. C. lanceolata were subjectedto preliminary antitumer screening test with Sarcoma 180 ascites and screening on V-79 cell. Thisexperiments were conducted in accordance with the total packed cell volume method and cytotoxicity method.

• 한국임상수의학회지
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• 제20권1호
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• pp.115-120
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• 2003

This study was conducted to investigate the hematological changes of the dogs when administrated excessive amount of the Artemisia capillaris extract for a short period. In this experiment, clinically healthy dogs(n = 20, average weight = 7.68 kg) were divided into 2 groups : Artemisia capillaris rude juice extract group(CR) and concentrated extract group(CE). They were inserted stomach tube and administrated the extracts (5 ml/kg) for 8 days. The followings are the results of this experiment.: 1). The number of red blood cell(RBC) was significantly (p<0.05) decreased in CE group on day 4, 5, 6, whereas CJE group showed significantly (p<0.05) difference on day 5. 2). The packed cell volume(PCV) and hemoglobin concentration were significantly (p<0.05) decreased in both groups during experimental periods. 3). The met hemoglobin was significantly (p < 0.05) increased from 12 hrs after administration to day 8 in CJE group wheres, it was significantly (p<0.05) increased on day 6,7 in CE group.

• Parasites, Hosts and Diseases
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• 제58권5호
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• pp.565-569
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• 2020

This report describes the first clinical case of a transfusion-associated Mycoplasma haemocanis infection in a dog in Korea. A 6-year-old male Maltese underwent a red blood cell transfusion for idiopathic immune-mediated hemolytic anemia. Eighteen days after the blood transfusion, the recipient's packed cell volume decreased and basophilic organisms were found on erythrocytes. A polymerase chain reaction and sequential analysis showed that both the donor dog and recipient dog had M. haemocanis. Six weeks after doxycycline administration, no organisms were detected and the recipient's anemia had improved.

• 상하수도학회지
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• 제27권4호
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• pp.439-444
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• 2013

Excessive phosphorus in aquatic systems causes algal bloom resulting in eutrophication, DO depletion, decline in recreational value of water and foul tastes. To treat wastewater containing phosphorus including effluent of wastewater treatment plant, the continuous experiments were performed by using electrochemical way. The spherical ZVI and silica sand which act as physical filter are packed at appropriate volume ratio of 1:2. Electric potential is applied externally which can be changed as per the operational requirement. The results indicate that optimum hydraulic retention time of 36 minutes (10 mL/min at 1 L reactor) was required to meet the effluent standards. Lower concentrations of phosphorus (<10 mg/L as phosphate) were removed by precipitation by contact with iron. Thus, additional electric potential was not required. In order to remove high concentration phosphorus around 150 mg/L as phosphate, external electric potential of 600 V was applied to the reactor.