• Title/Summary/Keyword: PWE3

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Are Current Aspergillus sojae Strains Originated from a Native Aflatoxigenic Aspergillus Species Population Also Present in California?

  • Perng-Kuang Chang;Sui Sheng T. Hua
    • Mycobiology
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    • v.51 no.3
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    • pp.139-147
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    • 2023
  • Aspergillus sojae has long been considered a domesticated strain of Aspergillus parasiticus. This study delineated relationships among the two species and an Aspergillus PWE36 isolate. Of 25 examined clustered aflatoxin genes of PWE36, 20 gene sequences were identical to those of A. sojae, but all had variations to those of A. parasiticus. Additionally, PWE36 developmental genes of conidiation and sclerotial formation, overall, shared higher degrees of nucleotide sequence identity with A. sojae genes than with A. parasiticus genes. Examination of defective cyclopiazonic acid gene clusters revealed that the PWE36 deletion pattern was identical only to those of A. sojae. Using A. sojae SMF134 genome sequence as a reference, visualization of locally collinear blocks indicated that PWE36 shared higher genome sequence homologies with A. sojae than with A. parasiticus. Phylogenetic inference based on genome-wide single nucleotide polymorphisms (SNPs) and total SNP counts showed that A. sojae strains formed a monophyletic clade and were clonal. Two (Argentinian and Ugandan) A. parasiticus isolates but not including an Ethiopian isolate formed a monophyletic clade, which showed that A. parasiticus population is genetically diverse and distant to A. sojae. PWE36 and A. sojae shared a most recent common ancestor (MRCA). The estimated divergence time for PWE36 and A. sojae was about 0.4 mya. Unlike Aspergillus oryzae, another koji mold that includes genetically diverse populations, the findings that current A. sojae strains formed a monophyletic group and shared the MRCA with PWE36 allow A. sojae to be continuously treated as a species for food safety reasons.

Portulaca oleracea L. Extract Lowers Postprandial Hyperglycemia by Inhibiting Carbohydrate-digesting Enzymes (쇠비름(Portulaca oleracea L.) 추출물의 탄수화물 소화 효소 저해와 식후 고혈당 완화 효과)

  • Park, Jae-Eun;Han, Ji-Sook
    • Journal of Life Science
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    • v.28 no.4
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    • pp.421-428
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    • 2018
  • Postprandial hyperglycemia plays an important role in the development of Type 2 Diabetes and diabetic complications. Controlling postprandial hyperglycemia is the most important factor for reducing the risks of diabetic complications in Type 2 diabetic patients. This study was designed to determine whether Portulaca oleracea L. extract suppresses the activation of carbohydrate-digesting enzymes, and lowers postprandial hyperglycemia in diabetic mice through streptozotocin. P. oleracea was extracted with either 80% ethanol (PEE) or water (PWE), and the extract solutions were concentrated. The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibition assays were performed using the chromogenic method. Normal mice and STZ-induced diabetic mice were orally treated with PEE, PWE (300 mg/kg of body weight) or acarbose (100 mg/kg of body weight), with soluble starch (2 g/kg of body weight). The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibitory effectiveness by PEE were markedly more effective than PWE, and both extracts indicated a higher effectiveness than the acarbose (positive control). The rise in postprandial blood glucose due to starch loading was markedly inhibited in the PEE group when compared to the control group in diabetic and normal mice. Furthermore, the area under the concentration-time curve values were markedly declined by the PEE injection in the diabetic group when compared to that exerted for the control group. These results demonstrate that P. oleracea extracts lower postprandial hyperglycemia by inhibiting carbohydrate-digesting enzymes, and that the ethanol extract is more efficacious than the water extract.

The Worked Example Effect using Ill-defined Problems in On-line Learning : Focus on the Components of a Worked Example (온라인 학습에서 비구조화된 문제에 대한 해결된 예제 효과)

  • Kyun, Suna;Lee, Jae-Kyung;Lee, Hyunjeong
    • Journal of Information Technology Services
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    • v.14 no.1
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    • pp.129-143
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    • 2015
  • This study has two goals. The first goal is to investigate whether worked examples are effective in the ill-defined domain with on-line learning and the second goal is to find out which components (conceptual or procedural knowledge) of worked examples are effective factor at the given learning environment. We carried out three experiments in which Korean undergraduate or graduate students were working in three or four conditions of worked examples (CWE, PWE, CPWE, or the control group). While experiment 1 conducted in on-line learning environment did not find any effect and difference among groups and also any logical reason for those results, experiment 2 conducted in completely controlled laboratory setting with less knowledgeable students showed the clear difference among groups by the order CPWE, PWE, and CWE. Experiment 3 in which highly knowledgeable and motivated students were presented the same materials in more controlled on-line learning environment indicated the difference among groups by the order CWE, CPWE, and PWE. The results were discussed within the framework of cognitive load theory.

Study of Mössbauer Spectroscopy for Iron Oxides Synthesized by Pulsed Wire Evaporation (PEW) (전기선폭발법으로 제조된 철산화물의 뫼스바우어분광연구)

  • Uhm, Young Rang
    • Journal of the Korean Magnetics Society
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    • v.24 no.5
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    • pp.135-139
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    • 2014
  • Iron-oxide nanopowders were synthesized by a pulsed wire evaporation (PWE) in various ambient gas conditions. SEM measurement indicates that the spherical iron nanoparticles are about 50 nm in diameter. The phase analysis for the produced iron-oxide powders was systematically investigated by using $M\ddot{o}ssbauer$ spectra and the results show that classified phases of $Fe_2O_3$ and $Fe_3O_4$ can be controlled by regulating the oxygen concentration in the mixed gas during the PWE process. A quadrupole line on the center of $M\ddot{o}ssbauer$ spectrum represents the superparamagnetic phase of 12 % from ${\gamma}-Fe_2O_3$ phase.

Fabrication of Ni-free Fe-based Alloy Nano Powder by Pulsed Wire Evaporation in Liquid: Part I. Effect of Wire Diameter and Applied Voltage (액중 전기선 폭발법에 의한 Ni-free Fe계 나노 합금분말의 제조: 1. 합금 wire의 직경 및 인가 전압의 영향)

  • Ryu, Ho-Jin;Lee, Yong-Heui;Son, Kwang-Ug;Kong, Young-Min;Kim, Jin-Chun;Kim, Byoung-Kee;Yun, Jung-Yeul
    • Journal of Powder Materials
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    • v.18 no.2
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    • pp.105-111
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    • 2011
  • This study investigated the effect of wire diameter and applied voltage on the fabrication of Ni-free Fe-based alloy nano powders by employing the PWE (pulsed wire evaporation) in liquid, for high temperature oxidation-resistant metallic porous body for high temperature particulate matter (or soot) filter system. Three different diameter (0.1, 0.2, and 0.3 mm) of alloy wire and various applied voltages from 0.5 to 3.0 kV were main variables in PWE process, while X-ray diffraction (XRD), field emission scanning microscope (FE-SEM), and transmission electron microscope (TEM) were used to investigate the characteristics of the Fe-Cr-Al nano powders. It was controlled the number of explosion events, since evaporated and condensed nano-particles were coalesced to micron-sized secondary particles, when exceeded to the specific number of explosion events, which were not suitable for metallic porous body preparation. As the diameter of alloy wire increased, the voltage for electrical explosion increased and the size of primary particle decreased.

A Study on Level of Service of Pedestrian Facility in Transfer Stations at Urban Railroad (도시철도 환승역의 환승보행시설의 서비스수준에 관한 연구)

  • Jang, Seong-Yong;Han, Sung-Yoeb;Kim, Si-Gon
    • Journal of the Korean Society for Railway
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    • v.13 no.3
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    • pp.339-348
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    • 2010
  • The government continues to make efforts to improve effectiveness, integration and connectivity of public transportation systems. But there have been only a few studies about it. This paper addresses the quality evaluation of pedestrian facility service in transfer stations at urban railroad. LOS (Level Of Service) model of urban railroad by facility based on the body ellipse and density recalculated considering the recent Korean body standard and the suggested Pedestrian Moving Equivalent (PME) and Pedestrian Waiting Equivalent (PWE). For LOS model for the ticket booths and ticket vending machines, queuing theory has been applied to determine LOS of Pedestrians. And the importance weights among the pedestrian facilities is obtained by applying AHP analysis. Lastly, this paper shows and discusses the evaluation results of overall LOS of pedestrian facilities of five urban railroad transfer stations in Seoul.

Studying on Mobile backhaul Based on FTTH network (FTTH 인프라를 이용한 이동통신 기지국 백홀 제공방안 연구)

  • Kim, Geun-Young;Kim, Jin-Hee;Woo, Kyung-Il
    • 한국정보통신설비학회:학술대회논문집
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    • 2009.08a
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    • pp.78-80
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    • 2009
  • In this paper, we have described the advantages of fixed mobile convergence access network based on FTTH. Also, we have investigated the possibility of mobile backhaul based on FTTH network combined TOM over IP emulation and adaptive clock recovery technologies, and verified successful transport of both E1 TDM traffic and Clock through the packet based PON network. within the allowable tolerance.

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Bandwidth Improvement for a Photonic Crystal Optical Y-splitter

  • Danaie, Mohammad;Kaatuzian, Hassan
    • Journal of the Optical Society of Korea
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    • v.15 no.3
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    • pp.283-288
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    • 2011
  • In this study, a wide-band photonic crystal Y-splitter for TE modes is proposed. A triangular lattice of air holes etched in a GaAs slab is used as the platform. In order to numerically analyze the structures, plane wave expansion (PWE) and finite difference time domain (FDTD) methods are used. In comparison with the structures reported in the literature, the proposed topology has a less complexity while it provides more than 100nm bandwidth. The simplicity of the design, its high transmission ratio and its wide bandwidth makes it a suitable choice for the implementation of photonic crystal integrated circuits.

Effect of Atmospheric Gas on the Size and Distribution of Cu Nano Powders Synthesized by Pulsed Wire Evaporation Method (전기 폭발법에 의해 제조된 나노 구리 분말의 크기와 분포에 미치는 조업 가스의 영향)

  • ;;;Y. A. Kotov
    • Journal of Powder Materials
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    • v.11 no.3
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    • pp.210-216
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    • 2004
  • The possibility to decrease agglomeration of Cu nano powders and their separation during pulsed wire evaporation (PWE) process was investigated by controlling the working gas system, i.e., the design of the gas path, the type and pressure of the atmospheric gas. As a result, it was possible to choose the optimal design of the gas path providing large specific surface area and high degree of separation of the synthesized Cu nano powders. It was also shown that an Ar+10∼50$N_2$ mixture can be used in production of Cu nano powders, which do not react with nitrogen.

Study of Magnetic Fe2O3 Nano-particles Synthesized by Pulsed Wire Evaporation (PWE) Method (전기폭발법에 의해 제조된 자성 Fe2O3 나노 분말의 자기적 특성연구)

  • 엄영랑;김흥회;이창규
    • Journal of Powder Materials
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    • v.9 no.5
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    • pp.341-345
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    • 2002
  • Nanoparticles of $Fe_2O_3$ with a mean particle size of 4-30 nm have been prepared by a pulsed wire evaporation method, and its structural and magnetic properties were studied by SQUID magnetometer and Mossbauer spectroscopy. From the main peak intensity of XRD and absorption rate of Mossbauer spectrum, the amounts of $\gamma-Fe_2O_3$ and $\alpha-Fe_2O_3$ in as-prepared sample are about 70% and 30%, respectively. The coercivity (53 Oe) and the saturation magnetization (14 emu/g) are about 20% of those of the bulk $\gamma-Fe_2O_3$. The low value of coercivity and saturation magnetization indicate that the $\gamma-Fe_2O_3$ phase nearly shows the spin glass-like behavior. Analysis of the set of Mossbauer spectrum indicates a distribution of magnetic hyperfine fields due to the particle size distribution yielding 20 nm of average particle size. The magnetic hyperfine parameters are consistent with values reported of bulk $\gamma-Fe_2O_3$ and $alpha-Fe_2O_3$. A quadrupole line on the center of spectrum represents of superparamagnetic phase of $\gamma-Fe_2O_3$ with a mean particle size of 7 nm or below.