• Journal of Microbiology and Biotechnology
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• v.32 no.8
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• pp.1047-1053
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• 2022

When ptsG, a glucose-specific phosphotransferase system (PTS) component, is deleted in Escherichia coli, growth can be severely poor because of the lack of efficient glucose transport. We discovered a new PTS transport system that could transport glucose through the growth-coupled experimental evolution of ptsG-deficient E. coli C strain under anaerobic conditions. Genome sequencing revealed mutations in agaR, which encodes a repressor of N-acetylgalactosamine (Aga) PTS expression in evolved progeny strains. RT-qPCR analysis showed that the expression of Aga PTS gene increased because of the loss-of-function of agaR. We confirmed the efficient Aga PTS-mediated glucose uptake by genetic complementation and anaerobic fermentation. We discussed the discovery of new glucose transporter in terms of different genetic backgrounds of E. coli strains, and the relationship between the pattern of mixed-acids fermentation and glucose transport rate.

• Textile Coloration and Finishing
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• v.31 no.2
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• pp.118-126
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• 2019

This study aims to investigate the effect of polytriazolesulfone(PTS) addition on fracture toughness of diglycidyl ether of bisphenol A(DGEBA) and 4,4'-diaminodiphenylsulfone(DDS). Various amounts of PTS were added to DGEBA/4,4'-DDS in diazide and dialkyne monomer forms and polymerized during the epoxy curing process. Fracture toughness(K1C), tensile properties and thermal stability of the PTS added epoxy resin were evaluated and compared with those of PES, the conventional high Tg toughening agent, added epoxy resin. Fracture toughness of the PTS added epoxy resin was dramatically improved up to 133%, as the amount of PTS added increased, whereas that of the PES added epoxy resin was improved by only 67%. The tensile strength of PTS added DGEBA/4,4'-DDS was similar to the epoxy resin without PTS and tensile modulus was improved by 20%. And thermal stability of the PTS added epoxy resin was improved up to 14%. Therefore, PTS addition to DGEBA/4,4'-DDS, as a toughening agent, is very effective way to improve its fracture toughness without any lowering in other properties.

• Proceedings of the Korea Contents Association Conference
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• 2005.05a
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• pp.458-463
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• 2005

OFDM communication system is effective for the high data rate transmission in the frequency selective fading channel. Since OFDM has high PAPR, OFDM signal may be distorted by the nonlinear HPA. In this paper, we propose an combined SLM and PTS method for reducing the PAPR in OFDM communication system. Proposed method increased some system complex in comparison with exiting method. But we made sure of high efficiency in the case of reducing the PAPR. As a result of using the 16-QAM modulation and increasing the carrier number in the SLM, PTS method and the SLM-PTS combined method, SLM-PTS combined method is more an effective method of reducing 3.5 dB PAPR than exiting OFDM system when this method is M, L=3, D=128, 256 in 10-3 BER.

• Applied Biological Chemistry
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• v.42 no.4
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• pp.293-297
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• 1999

The pts operon, which encodes several factors in the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) of Escherichia coli, has multiple promoters which respond to different signals to facilitate quick adaptation to changes in growth conditions. The influence of an 1 kbp DNA region upstream of the pts P0 promoter on pts expression was studied in vitro by employing the DNA templates containing both P0 and P1 promoter with or without the 1 kbp upstream DNA region for in vitro transcription assay. The 1 kbp DNA region upstream of the pts P0 promoter, however, had no effect on pts transcription in vitro. The intracellular concentration of cAMP was measured when cells were grown in the presence of glucose, mannose, or mannitol. The transcription of P0 was increased maximally in the presence of glucose even though the concentration of cAMP in the condition was lowest while the transcription from the P1b was highest when cells were grown in the presence of mannose or mannitol even though the intracellular concentration of cAMP was lower than cells grown in the absence of the sugar. These results suggest the possibility of the existence of a glucose inducible repressor specific for the P0 promoter and a second repressor that is inducible by glucose, mannose and mannitol specific for the P1 promoter.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.8
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• pp.1165-1171
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• 2010

The OFDM system has better characteristics in transmission rate, power efficiency, bandwidth efficiency, impulse-noise immunity, and narrow band interference immunity etc. in comparison with other conventional systems. However, high PAPR of an OFDM signals causes some serious non-linear processing of RF amplifier. And performance of the communication system gets worse. Therefore, various methods reducing PAPR of an OFDM skills such as the clipping method, block coding method, and phase rotation method etc. have been researched. In this paper, we propose a high-speed adaptive PTS method which eliminates high PAPR. And we compare the proposed method with other conventional methods. The proposed method has decreased quantity of calculation compare with an adaptive PTS method. Of course, The more its calculation amount is decreased, the more its BER characteristic is not better than an adaptive PTS method. However, keeping up satisfactory BER performance, we highly improved calculation amount of a PTS method.

• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.829-835
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• 2000

The ptsH and ptsI genes of Lactococus lactis subsp. lactis ATCC 7962 (L. lactis 7962), encoding the general proteins of phosphotransferase system (PTS) components, HPr and enzyme I, respectively, were cloned and characterized. A 1.3 kb PCR product was obtained using a primer set that was hybridized to the internal region of the L. lactis 7962 pts HI genes and then subcloned into a low-copy number vector, pACYC184. The 5' upstream and 3' downstream region from the 1.3 kb fragment were subsequently clone using the chromosome walking method. The complete ptsHI operon was constructed and the nucleotide sequences determined. Two ORFs corresponding to HPr (88 amino acids) and enzyme I (575 amino acids) were located. The ptsHI genes of L. lactis 7962 showed a very high homology (84-90%) with those genes from other Gram-positive bacteria. A primer extension analysis showed that the transcription started at either one of two adjacent bases upstream of the start codon. Using a Northern analysis, two transcripts were detected; the first, a 0.3 kb transcript corresponding to ptsH and the second, a 2 kb transcript corresponding to ptsH and ptsI. The transcription level of ptsH was higher than that of ptsI. The concentration of the ptsH transcript in cells grown on glucose was similar to that in cells grown on lactose, yet higher than that in cells grown on galactose. The ptsI transcript was scarcely detected in cell grown on lactose or galactose. The ptsI transcript was scarcely detected in cells grown on lactose or galactose. The results of a sequence analysis and Northern blot confirmed that the ptsH and ptsI genes of L. lactis 7962 were arranged in an operon like other known ptsHI genes and the expression of the ptsHI genes was regulated at the transcriptional level in response to the carbon source.

• ETRI Journal
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• v.43 no.2
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• pp.209-220
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• 2021

The filtered orthogonal frequency division multiplexing (F-OFDM) system has been recommended as a waveform candidate for fifth-generation (5G) communications. The suppression of out-of-band emission (OOBE) and asynchronous transmission are the distinctive features of the filtering-based waveform frameworks. Meanwhile, the high peak-to-average power ratio (PAPR) is still a challenge for the new waveform candidates. Partial transmit sequence (PTS) is an effective technique for mitigating the trend of high PAPR in multicarrier systems. In this study, the PTS technique is employed to reduce the high PAPR value of an F-OFDM system. Then, this system is compared with the OFDM system. In addition, the other related parameters such as frequency localization, bit error rate (BER), and computational complexity are evaluated and analyzed for both systems with and without PTS. The simulation results indicate that the F-OFDM based on PTS achieves higher levels of PAPR, BER, and OOBE performances compared with OFDM. Moreover, the BER performance of F-OFDM is uninfluenced by the use of the PTS technique.

• The Journal of the Korea Contents Association
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• v.6 no.1
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• pp.1-7
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• 2006

This paper proposes a new method that reduces the complexity of the combined system, which consists of the selective mapping(SLM) and the partial transmit sequence(PTS) in the Orthogonal Frequency Division Multiplexing(OFDM). In order to reduce the Peak-to-Average Power Ratio(PAPR), many techniques have been developed such as SLM and PTS. and their combined systems. However the method that simply combines PTS and SLM to improve the effectiveness of a system increases the complexity of calculation. The scheme employed in this research suggests a system that combines PTS and SLM reducing the complexity via the N($N=2^n$)-point inverse fast Fourier transforms(IFFT), which uses the decimation in time domain not only to improve PAPR but also to reduce the amount of calculation. The proposed method reduces the complexity by multiplying a phase sequence and a subblock index to the data sequences in the middle of IFFT, which yields an optimal sequence with minimal PAPR. The superiority of the proposed method is demonstrated by simulation results and analysis.

• Journal of Navigation and Port Research
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• v.29 no.8 s.104
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• pp.755-761
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• 2005

In this paper, we propose a high-speed adaptive PTS method which eliminates high PAR (Peak-to-Average Power Ratio) and we compare the proposed method with other conventional methods. In addition, we have designed a combined type SLM-PTS scheme to reduce PAR and evaluate the performance. The system used for evaluating PAR performance can be constructed as COFDM (Coded Orthogonal Frequency Division Multiplexing) applying ETD(Enhabced Time Diversity)-Turbo coding scheme. All the analyses in this paper are focused on the system characteristics according to IFFT's point and modulation method and the performance evaluation are based on the PAR reduction rates. As a result, the SLM-PTS combination method reveals good PAR reduction rate and remarkable reduction in the amount of calculations. Especially, in the case of combine-3 scheme, we can achieve approximately $3.7\~3.9$ dB PAR reduction on a basis of 10-5 BER level. Moreover, we can eliminate the side information in COFDM system because of its adaptive characteristics in evaluating PAR reduction rate, so that the additional errors can be omitted.

• Journal of Microbiology and Biotechnology
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• v.16 no.5
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• pp.795-798
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• 2006

The phosphoenolpyruvate-dependent carbohydrate phosphotransferase system (PTS) is responsible for the simultaneous transfer and phosphorylation of various carbon sources in Escherichia coli. The ptsG gene encoding the enzyme $IICB^{Glc}$, the membrane component of the glucose-specific PTS, is repressed by Mlc and activated by the CRP cAMP complex; various other factors, such as Fis, FruR, and ArcA, are also known to be involved in ptsG regulation. Thus, in an attempt to discover a novel gene affecting the regulation of ptsG, a mutant with a decreased ptsG transcription in the presence of glucose compared with the wild-type strain was screened using transposon random mutagenesis. The mutant was found to have a transposon insertion in yhjV, a putative gene encoding a transporter protein whose function is yet unknown.