• 한국어류학회지
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• 제31권4호
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• pp.195-200
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• 2019

본 연구는 대구(Gadus macrocephalus) 정자의 동결보존 기술을 개발하기 위해 적정 동해방지제 및 동결보존 방법을 탐색하고, 동결보존된 정자를 해동하여 효과를 조사하고자 한다. 동결보존에 사용된 동해방지제는 demethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol, methanol 및 propylene glycol (PG)였으며, marine fish ringer's solution (MFRS)를 희석액으로 사용하였다. 적정 동결보존 방법을 탐색하기 위하여 동해방지제 농도별(10%, 20%), 평형시간(3분, 5분, 10분), 1차 동결(액체 질소 표면 위 3, 8, 12 cm)별로 총 90개 실험구를 설정하였다. 해동 후 가장 높은 정자 생존율을 보인 실험구는 PG 10%, 평형시간 3분, 1차동결 8 cm 방법으로 21.3±1.8%의 생존율을 보였다. 신선한 정자와 해동한 정자를 사용하여 인공수정시킨 수정란의 부화율에서 유의한 차이는 없었다.

• 한국가금학회지
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• 제40권3호
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• pp.197-205
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• 2013

동결 닭 원시생식세포의 생식계열 키메라를 이용한 생체에의 복원을 실용화하기 위해서는, 닭 원시생식세포의 동결보존기술의 향상에 의해 동결 및 융해 후의 많은 생존세포를 확보하는 것이 반드시 필요하다. 닭 원시생식세포는 배양 5.5일령의 닭 원시생식선으로부터 채취하고, MACS 방법에 의해서 순수 닭 원시생식세포를 분리했다. 15% 각각의 EG를 동결보호제로 사용한 처리군이 각 군의 농도에 상관없이 유의적(p<0.05)으로 PG 처리군보다 동결 및 융해 후의 세포의 생존율이 높음을 확인하였다. 특히, 10% EG 처리군에서 85.63%로 동일한 농도의 PG 처리군(66.81%)보다 유의적(p<0.05)으로 가장 높은 생존율을 보였다. 한편, 상업용 닭(한협3호)에서도 오계와 비슷한 경향의 결과를 확인하였다. 이상의 결과들로부터 유리화 동결에 있어서 가장 높은 생존율을 보인 10% EG이 최적의 동결 보호제로서 사용 가능함을 확인하였다.

• 멤브레인
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• 제29권2호
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• pp.88-95
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• 2019

올레핀은 석유화학산업에서 대부분의 물질의 근간이 되는 핵심적인 물질이며 특히 고분자 합성에 있어 매우 중요하다. 이러한 올레핀 물질을 효율적으로 분리/가공하는 공정은 산업발전에 있어 지대한 영향을 끼친다. 본 연구에서는 올레핀 물질 중 프로필렌 기체를 선택적으로 분리하는 고분자 복합막을 제조하여 투과 및 선택 성능을 증대시키고자 고투과성 매질인 poly(1-trimethylsilyl-1-propyne) (PTMSP)에 양친성 고분자를 이용하여 개질하였다. 또한 올레핀 분자와 상호작용이 있는 $AgBF_4$ 염 및 촉진수송을 극대화 시키기 위하여 이온성 액체인 $EMIM-BF_4$를 첨가하여 올레핀/질소 투과 분리 성능을 향상시켰다. 기존 PTMSP 복합막의 경우 굉장히 높은 자유부피를 가져 높은 기체 투과성능을 보이는 반면 투과시키고자 하는 기체에 대한 선택적인 분리 성능이 매우 떨어져 낮은 선택도를 보인다. 이를 극복하고자 양친성 고분자를 PTMSP 계면에 그래프트 공중합을 시켰으며 올레핀과 높은 상호작용을 보이는 $AgBF_4$ 염 및 $EMIM-BF_4$ 이온성 액체를 첨가하여 프로필렌/질소에 대한 선택도를 향상시켰다.

• 한국분무공학회지
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• 제19권1호
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• pp.19-24
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• 2014

In this study, the cooling performance of a liquid CPU cooler using the water/propylene glycol(PG)-based $Al_2O_3$ nanofluids is experimentally investigated. Water/PG-based $Al_2O_3$ nanofluids are manufactured by two-step method with ultrasonic energy for 10 hours. The volume fractions of the nanofluids are 0.25% and 0.35%. Thermal conductivity and viscosity of the nanofluids are measured to theoretically predict the thermal performance of the liquid CPU cooler using performance factor. Performance factor results indicate that the cooling performance of the liquid CPU cooler can be improved using the manufactured nanofluids. To evaluate the cooling performance of the liquid CPU cooler experimentally, temperature differences between ambient air and heater are measured for base fluid and nanofluids respectively. Based on the results, it is shown that performance of the liquid CPU cooler using $Al_2O_3$ nanofluids is improved maximum up to 8.6% at 0.25 Vol.%.

• 한국식품조리과학회지
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• 제33권2호
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• pp.198-208
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• 2017

Purpose: To improve the quality of basic gluten-free rice bread composed of white rice flour, salt, sugar, yeast, skim milk powder, olive oil, and water, the effects of transglutaminase (TGase), whey protein (WP), propylene glycol alginate (PGA), and hydroxypropylmethylcelluose (HPMC) were investigated. Methods: TGase, WP, PGA, and HPMC were added to rice flour cumulatively. The pasting properties of rice flour blends as well as volume, shape, color value, textural properties and sensory evaluation of basic rice bread (RB1) RB1+TGase (RB2), RB1+TGase+WP (RB3), RB1+TGase+WP+PGA (RB4), and RB1+TGase+WP+PGA+HPMC (RB5) were compared. Results: Consistency of rice batter increased upon addition of TGase, WP and PGA, and RB3 and RB4 had higher specific volumes than others. PGA improved volume, crumb air cell uniformity, and resilience but lowered elasticity and moistness of RB. HPMC increased, hardness, moistness and softness, and slightly reduced volume. Conclusion: Therefore, it is suggested that hydrocolloids, PGA and HPMC may be necessary to improve volume, crumb structure, textural properties and overall eating quality of gluten-free rice bread.

• Journal of Pharmaceutical Investigation
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• 제29권4호
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• pp.337-341
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• 1999

We have studied the stability and transdennal flux of prostaglandin $E_1\;(PGE_1)$ from various donor solutions through hairless mouse skin. Stability in HEPES buffer or in propylene glycol (PG) solution where enhancer (oleic acid (OA), propylene glycol monolaurate (PGML), transcutol (TC), ethanol (EtOH))s dissolved was investigated. $$PGE_1 was not stable in HEPES buffer. The concentration of $$PGE_1 decreased continuously for 7 days, and the degradation rate constant was $0.0028\;h^{-1}$, assuming first order reaction. The effect of current or penetration enhancer on the degradation was minimal. Percutaneous transport from HEPES buffer by passive or iontophoretic delivery without enhancer was close to nil. When OA or PGML was used together with PG, both passive and iontophoretic flux increased. PGML showed better enhancing effect than OA. Flux by cathodal delivery was about 2 times larger than that by passive delivery. Flux by anodal delivery was lower than that by passive delivery. TC and EtOH also increased the transdermal flux, but the effect was not as good as that observed when OA or PGML was used. These stability and flux data provide important information on how to formulate the patch, which will be the next step of this work, and on the polarity of current to use during iontophoresis.

• Macromolecular Research
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• 제9권5호
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• pp.292-295
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• 2001

A cross-linked solid polymer electrolyte was prepared by copolymerizing photochemically acrylonitrile (AN), oligo(ethylene glycol ethyl ether) methacrylate, oligo(ethylene glycol) dimethacrylate in the presence of lithium perchlorate as a lithium salt, ethylene carbonate-propylene carbonate as a mixed plasticizer, and poly(ethylene oxide) as a polymer matrix. The maximum ionic conductivity of the polymer electrolyte was 2.35$\times$10$\^$-3/ S/cm. The interface resistance of the polymer electrolyte was very low compared to that of the polymer electrolyte without AN. The former electrolyte was stable up to 4.3 V and the Ah efficiency was nearly 100% during the charge-discharge cycle.

• 폴리머
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• 제33권4호
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• pp.371-376
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• 2009

폴리(에틸렌 나프탈레이트) 공중합체를 합성하기 위하여 에틸렌글리콜과 함께 1,3-프로판디올을 혼합사용하여 폴리(에틸렌/프로필렌 나프탈레이트)를 합성하고 이를 유연 기판 소재로 적용하기 위한 물성을 살펴보았다. NMR 실험 결과, 에틸렌과 프로필렌 segment가 함께 공존하는 폴리(에틸렌/프로필렌 나프탈레이트)가 합성되었음을 확인하였다. 에틸렌과 함께 프로필렌 segment가 존재하는 경우 합성된 폴리에스터의 결정화가 일어나지 않으며 유리전이온도와 열분해온도는 감소함을 알 수 있다. 합성된 폴리(에틸/프로필렌 나프탈레이트)의 주사슬에 에틸렌 보다 상대적으로 긴 프로필렌이 공존함에 따라 주사슬 배향이 어려워 열팽창계수가 감소함에 따라 합성 시 1,3-프로판디올 첨가에 따른 다소간의 내열온도 감소에도 저수축 유연기판 소재로 적용 가능함을 알 수 있었다.

• 대한의생명과학회지
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• 제15권3호
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• pp.217-227
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• 2009

The present study was set to evaluate the effect of pomegranate extracts on improvement of the menopausal syndromes such as face flushing in ovariectomized rats by carrying out short- and long-term experiments. Pomegranate extracts used to feed rats were prepared from the pulp part which does not contain the rid of the pomegranate, and were dissolved in propylene glycol. From the short-term (16 days) experiment, it was clear that when the 25, 250, 1,250 mg/kg/day concentrations of pomegranate extracts were orally fed to ovariectomized rats, the body temperature of the rats in all the 3 groups were decreased with statistical significance compared to other control groups which were fed with propylene glycol only. Especially, the body temperature decreased by $2.7^{\circ}C$ compared to control groups even when the pomegranate extracts were fed at the low concentration of 25 mg/kg/day implying the usefulness of pomegranate extracts in improving face flushing troubles. In addition, the body weight of the groups fed with pomegranate extracts also decreased when compared to groups fed with only propylene glycol, and the results were also statistically significant. In case of the estradiol level in the blood of rats, the levels were somewhat higher in the groups fed with pomegranate extracts than the control groups, even though the difference was not statistically significant. As found from the results of the short-term experiment, in long-term experiment, the groups fed with pomegranate extracts showed statistically significant decrease in the body temperature and the body weight, whereas the increase of the estradiol levels in blood in each groups were statistically insignificant. During the short- and long-term experiments, no sign of toxicity was found in rats fed with pomegranate extracts indicating no toxic side effects of the pomegranate extracts when orally fed. The concentrations of pomegranate extracts 25, 250, 1250 mg/kg/day treated to ovariectomized rats in this study can be estimated to be 1.5, 15, and 75 g/day when treated to women whose body weight is 60 kg which is average for women with menopausal syndromes. Since even the 75 g/day of high concentration of pomegranate extracts did not show any toxicity in short- and long-term experiments, taking 1.5 g/day concentration of pomegranate extracts would be safe dose for not causing any side effects. Therefore, it can be concluded from the results of this study that taking 1.5 g/day of pomegranate extracts for certain period time will improve the menopausal syndromes including face flushing.

• 한국식품위생안전성학회지
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• 제32권5호
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• pp.404-410
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• 2017

HPLC-size-exclusion chromatography에 의해 가공식품에서 알긴산프로필렌글리콜의 함량을 분석하는 방법이 개발되었다. 알긴산프로필렌글리콜을 분석하기 위해 GF-7M HQ column과 LT-ELSD detector가 선정되었다. 알긴산프로필렌글리콜 분석을 위한 전처리 조건으로는 $20^{\circ}C$에서 150 rpm으로 3시간 동안 추출하는 방법이 선정되었다. 알긴산프로필렌글리콜을 5 농도(300, 500, 700, 1,000, and 1,500 mg/kg) 범위에서 검량선을 작성한 결과 직선성($R^2$)은 0.9873으로 측정되었다. HPLC system에 의한 알긴산프로필렌글리콜 분석시 검출한계(LOD) 및 정량한계(LOQ)는 각각 171.43 mg/kg 및 519.50 mg/kg이었다. Size-exclusion chromatography에 의해 얻은 회수율 및 변동 계수(coefficient of variation)는 각각 86.1~110.4% 및 4.1~13.5%이었다. 본 연구에서 개발된 HPLC-size-exclusion chromatography system을 적용하여 134 품목의 가공식품에서 알긴산프로필렌글리콜 함량을 분석하였다. 이 결과들은 이 방법이 가공식품에서 알긴산프로필렌글리콜 함량을 분석하는데 적용할 수 있는 방법이라는 것을 나타낸다.