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http://dx.doi.org/10.13103/JFHS.2017.32.5.404

Establishment of Analytical Method for Propylene Glycol Alginate in Food Products by Size-exclusion Chromatography  

Jeong, Eun-Jeong (Department of Food Science & Technology, Chonbuk National University)
Choi, Yoo-Jeong (Department of Food Science & Technology, Chonbuk National University)
Lee, Gunyoung (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
Yun, Sang Soon (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
Lim, Ho Soo (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
Kim, Yong-Suk (Department of Food Science & Technology, Chonbuk National University)
Publication Information
Journal of Food Hygiene and Safety / v.32, no.5, 2017 , pp. 404-410 More about this Journal
Abstract
An analytical method for determination of propylene glycol alginate (PGA) in food products was developed by HPLC-size-exclusion chromatography. The GF-7M HQ column and LT-ELSD detector were determined by considering the instrumental analysis conditions for PGA analysis. The pretreatment method for the analysis of PGA was suitable for 3 hr extraction at $20^{\circ}C$ and 150 rpm according to the extraction temperature. Linearity ($R^2$) for the analysis of PGA was 0.9873 at calibration curve range of 300, 500, 700, 1,000, and 1,500 mg/kg (5 points). The limit of detection and limit of quantification of PGA on HPLC system was 171.43 and 519.50 mg/kg, respectively. The accuracy and coefficient of variation obtained by size-exclusion chromatography were 86.1~110.4% and 4.1~13.5%, respectively. By applying the HPLC-size-exclusion chromatography system, it was possible to analyze the contents of PGA in 134 different types of food products.
Keywords
Propylene Glycol Alginate; Size-exclusion Chromatography; Food Additives; Analytical Method; Method Validation;
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Times Cited By KSCI : 1  (Citation Analysis)
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