• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.161-165
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• 2005

Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.

• Food Science and Biotechnology
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• v.15 no.1
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• pp.5-12
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• 2006

Degradation performance of environmentally friendly plastics that can be disintegrated by combination of sunlight, microbes in soil, and heat produced in landfills was evaluated for use in industries. Two multi-degradable master batches (MCC-101 and MCC-102 were manufactured, separately mixed with polyethylene using film molding machine to produce 0.025 mm thick films, and exposed to sunlight, microbes, and heat. Low- and high-density polyethylene (LDPE and HDPE) films containing MCC-101 and MCC-102 became unfunctional by increasing severe cleavage at the surface and showed high reduction in elongation after 40 days of exposure to ultraviolet light. LDPE and HDPE films showed significant physical degradation after 100 and 120 days, respectively, of incubation at $68{\pm}2^{\circ}C$. SEM images of films cultured in mixed mold spore suspension at $30^{\circ}C$ and 85% humidity for 30 days revealed accelerated biodegradation on film surfaces by the action of microbes. LDPE films containing MCC-l01 showed absorption of carbonyls, photo-sensitive sites, at $1710\;cm${-1}$ when exposed to light for 40 days, whereas those not exposed to ultraviolet light showed no absorption at the same frequency. MCC-101-based LDPE films showed much lower $M_w$ distribution after exposure to UV than its counterpart, due to agents accelerating photo-degradation contained in MCC-101.

• Journal of Fashion Business
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• v.19 no.6
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• pp.101-111
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• 2015

This study aims to analyze the current state of menswear in the domestic fashion market and the trend of new menswear brands. In addition to this, a comparative analysis of domestic and foreign menswear brands will be done to provide baseline data for the domestic brands in regards to strategic direction in the coming years. The study used reports published by Samsungdesign.net, which provided trends of 110 new brands from 2005 to 2014, in order to analyze the trends of new menswear brands launched during that period. Menswear brand information in the Korean Fashion Brand Annual, of which there were 168, were utilized to research the current state of the brands in the domestic market. As a results, menswear is expected to become more sensitive to trends and designs thanks to steady launchings of menswear brands within character casual zoning for the past 10 years and active brand launchings within contemporary zoning in the last 5 years. Also, as more brands are targeting men in their 20's as their main customers, it suggests that menswear brands are striving for a younger mindset. In terms of pricing, prestige and budget line launchings they seem to be dwindling, while bridge brands are increasing. This shows that customers are demanding luxury items at a reasonable price. Since there are higher ratio of foreign brands in dress shirts and contemporary zoning, domestic brands need to develop and produce more high quality clothes within these zonings.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.68-75
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• 2000

D-Tagatose is a potential bulking agent in food as a non-calorific sweetener. To produce D-tagatose from cheaper resources, plasmids harboring the L-arabinose isomerase gene (araA) from Escherichia coli was constructed because L-arabinose isomerase was previously suggested as an enzyme that mediates the bioconversion of galactose to tagatose as well as that of arabinose to ribulose. In the cultures of recombinant E.coli with pTC101, which harboring araA of E.coli, tagatose was produced from galactose in 9.9 % yield. The enzyme extract of E.coli containing pTC101 also converted galactose into tagatose in 96.4 % yield. For the economic production of D-tagatose, an L-arabinose isomerase of E.coli was immobilized using covalent binding on agarose. While the free L-arabinose isomerase produced tagatose with the rate of 0.48 mg/U$.$day, the immobilized one stably converted galactose into average 7.5 g/l$.$day of tagatose during 7 days with higher productivity of 0.87 mg/U$.$day. In the scaled up immobilized enzyme system, 99.9 g/l of tagatose was produced from galactose with 20 % equilibrium in 48 hrs. The process was stably repeated additional 2 times with tagatose production of 104.1 and 103.5 g/l.

• Environmental Engineering Research
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• v.12 no.3
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• pp.101-108
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• 2007

Fixation of lead contaminants in the solidification/stabilization using Portland cement has been investigated by X-ray diffraction, scanning electron microscopy and compressive strength. The presence of lead was observed to produce lead carbonate sulfate hydroxide ($Pb_4SO_4(CO_3)_2(OH)_2$), lead carbonate hydroxide hydrate ($3PbCO_3{\cdot}2Pb(OH)_2{\cdot}H_2O$) and two other unidentified lead salts in cavity areas and was observed to significantly retard the hydration of cement. By 28 days, howevere, the XRD peaks of most of the lead precipitates have essentially disappeared with only residual traces of lead carbonate sulfate hydroxide and lead carbonate hydroxide hydrate evident. After 28 days of curing, hydration appears well advanced with a strong portlandite peak present though C-S-H gel peaks are not particularly evident. Lead species produced with the dissolution of lead precipitates are fixed into the cement matrix to be calcium lead silicate hydrate (C-Pb-S-H) during cement-based solidification.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.27 no.2
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• pp.101-107
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• 2021

Time-temperature integrators (TTIs) based on aqueous enzyme solutions produce transparent colors which lead to difficulty in distinguishing its color change by naked eye. In this present study, this issue has been solved by increasing the opacity of laccase-based TTI without changes in the kinetics (same zero-order reaction) and temperature dependency (similar Arrhenius activation energy values) of the color change. The opacity was increased by introducing TiO₂, latex, BaSO₄, or ZnO, in combination with a hydrocolloid (xanthan gum, acacia gum, pectin, and CMC) into the TTI system. The combination of TiO₂ and xanthan gum was the best. This finding broadened the advantages of laccase-based TTI to more practical uses for consumer convenience.

• Journal of Fashion Business
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• v.27 no.4
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• pp.101-114
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• 2023

This study aimed to create globally acceptable Hanbok-inspired work uniforms, by developing practical designs and providing useful resources for incorporating Hanbok characteristics into office attire. First, to establish the elements that the Hanbok work uniform should have, the definition and characteristics of service clothes and preferences for the process of modernization of traditional Hanbok were reviewed through a review of the preceding studies. For the Hanbok work uniform design, a total of 4 pieces, 2 for each male and female, were planned, a schematic diagram was created, and the design was modified after evaluation by fashion design experts. I made a pattern of the modified design with a CAD program, imported it into CLO 3D to produce a virtual costume, and confirmed the final design draft. Work uniform design has symbolism, functionality, and aesthetics as its characteristics. The functionality of the Hanbok work uniform designed in this study is suitable for the season, and it was designed to increase the efficiency of work when worn for a long time. The design was focused on the development of materials and patterns in consideration of comfort and activity. The esthetic nature of hanbok-inspired work clothes was intended to reveal details that could highlight the silhouette of the human body and reflect the beauty of hanbok.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.1
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• pp.101-106
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• 2012

The press bulging process is very useful and productive method to produce round-type mechanical components which have not been able to be manufactured because of limitation of the conventional press technology. The application of the press bulging process has expanded very quickly in the hydraulic and electronic industry and more recently it has been used to produce other mechanical parts such as the automobile and shipping parts. This expanding application also has brought some unsolved problems and leads many researchers to put their effort into the die design of the press bulging process. In this study, to obtain the optimum die shape for the press bulging process, various process parameters have been considered such as corner radius, bulging height, pressing length, and forming load, etc. The main interest of this paper is to verify the press bulging process which has more than 4.0 in height-length ratio. From this aspect, Finite Element analysis shows great ability to simulate the precise deformation process and gives us manufacturing database. Consideration of strain, stress, and strain-rate for the various cases has been also taken to keep the forming load within a particular range.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.434-442
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• 2012

The purpose of this research is to propose policy of domestic timber supply expansion. To recommend polices and draw the priority orders, this study has used Delphi technique and Analytic Hierarchy Process. It has executed to recommend policies of domestic timber supply expand by dividing produce, distribution and consumption parts. As the result of Delphi technique and AHP, 'Infrastructure for domestic timber produce (.239)', 'regional domestic timber gathering complex construction (.202)' and 'a public institution domestic timber use obligation (.126)' policies are derived.

• Journal of Embryo Transfer
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• v.14 no.1
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• pp.31-37
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• 1999

The objective of this study was to optimize the selection of sperm sources, optimal culture systems and vitrification method depends on sperm sources. The oocytes were inseminated with either KPN 105, 114, 191, SNU 101, 102, 103 or epididymis and then embryos inseminated were cultured in oviductal cell co-culture or HECM-6 as defined me dium. The blastocysts produced were pooled according to sperm sources as KPN, SNU or epididymis and then vitrified by OPP vitrification method. The results obtained were as follows: 1. The cleavage(86.2 or 84.7%) and development rates to blastocyst (30.6 or 32.0%) were not significantly different between oviductal cell co-culture or HECM-6 culture systems(P<0.05). 2. To determine the optimal sperm sources for using IVF in this system, cleavage rates in KPN 191 and SNU 101 (74.2, 55.8%) were significantly lower rather than those in KPN 105, 114, SNU 102, 103 or epididymis (86.7, 85.1, 89.8, 85.5 or 81.2%), but development rates to blastocyst in KPN 114, SNU 103 or epididymis sperm (30.0, 33.0 or 28.6%) were significantly higher rater than those in KPN 105, 191, SNU 101, 102(21.4, 15.4, 14.9 or 25.4%), respectively (P<0.05). 3. The blastocysts produced were pooled according to sperm sources as KPN, SNU or epididymis and then vitrified by OPP vitrification method. The survival rates were not significantly different among sperm sources (89.6%: 43/48 ; 90.1%: 46/51 ; 83.3% : 20/24). These results obtained indicate that the defined medium, HECM-6, could be use to produce of IVP bovine embryos. Since the frozen semen must be required to maintain of unvariation data in IVP embryo production system, KPN 114 and SNU 103 produced in our laboratory were useful for this purpose. The blastocysts produced by different sperm sources as KPN, SNU or epididymis were vitrified by OPP vitrification method and survived very high rates. The OPP vitrification method could be susceptibility to use of IVP bovine blastocyst embryos.