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http://dx.doi.org/10.5010/JPB.2005.32.3.161

The Use of Glufosinate as a Selective Marker for the Transformation of Cucumber (Cucumis sativus L.)  

Cho Mi-Ae (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Song Yun-Mi (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Park Yun-Ok (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ko Suck-Min (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Min Sung-Ran (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Liu Jang-Ryol (Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KIRBB))
Choi Pil-Son (Department of Medicinal Plant Resources, Nambu University)
Publication Information
Journal of Plant Biotechnology / v.32, no.3, 2005 , pp. 161-165 More about this Journal
Abstract
Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.
Keywords
Agrobacterium strains; ${\beta}$-glucuronidase (GUS); leaf painting assay; transgenic cucumber;
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Times Cited By KSCI : 1  (Citation Analysis)
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