• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.844-850
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• 2000

The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggsting that celluar recepators for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was locaized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS1 ligands, thereby indicating an energy-dependent receptor-mediated endocytotic pathway. Accordingly, the interaction of HBV-pres! with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.

• Food Science of Animal Resources
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• v.42 no.4
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• pp.557-565
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• 2022

The purpose of this research was to examine the effectiveness of pre-cooking treatments on the quality characteristics of ready-to-eat (RTE) empal gentong. Raw beef meat was pre-cooked in water bath at 90℃ for 0 min (C), 10 min (T1), 20 min (T2), and 30 min (T3) prior to retorting process at 121℃ and pressure at 70,000 Pa. Results showed that pre-cooking treatments in all treated samples could reduce fat contents in empal gentong's meat by 0.02% (T1), 0.28% (T2), and 1.13% (T3) respectively. Highest precooking time tends to increase the pH and CIE a^* values. However, CIE b^* values, water holding capacity, and sensory analysis were not affected by pre-cooking duration which must have been affected by sterilization process after pre-cooking. In conclusion, pre-cooking treatment before sterilization in producing empal gentong is a probable technique to reduce its fat content and improve its physical quality. A specific treatment at 90℃ for 10 min is recommended to achieve optimum quality of RTE empal gentong's meat.

• Journal of the Mineralogical Society of Korea
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• v.27 no.3
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• pp.115-124
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• 2014

In order to leach Au and Ag from gold-silver bearing sulfide concentrate, the sulfide concentrate was ground in a ball mill for a dry pre-treatment and a wet pre-treatment process. Mineralogical studies and thiourea leaching experiments were carried out with the pre-treated sulfide concentrate. The results of the pre-treatment with the concentrate samples showed the mean particle size and iso-electrical potential was smaller in the dry pre-treatment sample than in the concentrate sample, and the contents was lower in the wet pre-treatment sample than in the dry pre-treatment sample. In XRD analysis, amorphous properties were only shown in the wet pretreatment sample. The results of the concentrate sample leaching experiments showed that the best Au, Ag leaching parameters were when the addition of thiourea was at a 1.0 g concentration, ferric sulfate was 1.0 M, sulfuric acid was 2.0 M and the leaching temperature was at $60^{\circ}C$. The Au, Ag leaching rate was always much greater and faster with the wet pre-treatment samples than with the dry pre-treatment samples. Accordingly, it is expected that more Au, Ag can be leached in an eco-friendly methodology using wet pre-treatment. The pre-treatment could be improved with an optimized grinding additive reagent and through researching grinding time in future non-cyanide processes.

• Applied Chemistry for Engineering
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• v.29 no.5
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• pp.613-619
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• 2018

In this study, various influent sludge pre-treatment methods and the internal recirculation of thickened sludge from effluents using a liquid/solid separation unit were adopted to investigate their effects on the sludge digestion and methane production in a combined mesophilic anaerobic and thermophilic aerobic sludge digestion process. A lab-scale combined sludge digestion process was operated during 5 phases using different feed sludge pre-treatment strategies. In phase 1, the feed sludge was pre-treated with a thermal-alkaline method. In contrast, in phases 2, 3 and 4, the internal recirculation of thickened sludge from the effluent and thermal-alkaline, thermal, and alkaline pre-treatment (7 days) were applied to the combined process. In phase 5, the raw sludge without any pre-treatment was used to the combined process. With the feed sludge pre-treatment and internal recirculation, the experimental results indicated that the volatile suspended solid (VSS) removal was drastically increased from phases 1 to 4. Also, the methane production rate with the thermal-alkaline pre-treatment and internal recirculation was significantly improved, showing an increment to 285 mL/L/day in phase 2. Meanwhile, the VSS removal and methane production in phase 5 were greatly decreased when the raw sludge without any pre-treatment was applied to the combined process. Considering all together, it was concluded that the combined process with the thickened sludge recirculation and thermal-alkaline pre-treatment can be successfully employed for the highly efficient sewage sludge reduction and methane gas production.

• Research in Mathematical Education
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• v.16 no.1
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• pp.31-50
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• 2012

The present study explored whether the implementation of focused activities (intervention programme) can enhance 22 pre-service mathematics teachers' proficiency in solid geometry thinking level as well as change for the better their feelings in this discipline. Over a period of 6 weeks the pre-service teachers participated in activities and diversified experiences with 3D shapes, using illustration aids and actual experience of building 3D shapes in relation to the various spatial thinking levels. The research objectives were to investigate whether the intervention programme, comprising task-oriented activities of solid geometry, enhance mathematics pre-service teachers' mastery of their geometric thinking levels as well as examine their feelings towards this discipline before and after the intervention programme. The findings illustrate that learners' levels of geometric thinking can be promoted, entailing control on higher thinking levels as well as a more positive attitude towards this field.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.691-694
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• 2005

This paper proposed 10Gbps driver with pre-emphasis for high speed transmitter. the proposed driver increase bandwidth using Ft doubler method and design driver block and pre-emphasis block in together. Pre-emphasis functionality confirmed to control VDS of current source o driver, not to control slew rate of termination resistor. The proposed driver is designed in a 1.5V/0.13um 1-poly, 5-metal CMOS mixed-signal process.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.20 no.1
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• pp.10-21
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• 2010

Equations of chordwise and flapwise bending motions for the vibration analysis of rotating pre-twisted blades having tapered cross section and setting angle are derived by using hybrid deformation variable modeling. The two motions are couples to each other due to the pre-twisted angle of the beam cross section. The derived equations are transformed into dimensionless forms in which dimensionless parameters are identified. The effects of the dimensionless parameters on the modal characteristics of rotating pre-twisted blades having tapered cross section and orientation angle are investigated. The eigenvalue loci veering phenomena are also investigated and discussed in this work.

• Journal of Korean Elementary Science Education
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• v.34 no.1
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• pp.58-71
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• 2015

Ability of understanding students' ideas and thinking is critical to teachers' professional growth. In this study, 'pedagogical reasoning' was conceptualized as teachers' reasoning ability of students' misconceptions and possible causes of misconceptions when they hear and observe students' science discourses. To investigate elementary pre-service teachers' pedagogical reasoning levels, children's science discourses on light reflection and electric circuit were video recorded and 43 pre-service elementary teachers were asked to identify children's misconceptions and possible causes of misconceptions after watching the video clips. In conclusion, pedagogical reasoning levels of pre-service elementary teachers were not high enough, showing significant difference in light reflection and electric circuit. Possible explanations of this difference and educational implications were discussed.

• Journal of Korean Elementary Science Education
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• v.37 no.1
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• pp.66-79
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• 2018

The purpose of this study is to explore how pre-service elementary teachers perceive and use smart learning environments. For this purpose, 23 pre-service elementary teachers who took theory and practice in a science education course were asked to develop lesson materials using smart tools and make a self-report questionnaire. These data were categorized in an instructional, exploratory, and interactive approach, depending on how they guided students to access knowledge and information. As a result of the study, pre-service teachers perceived the smart tools as the exploratory and interactive learning tools to be used for students to actively search for and interact with data and knowledge. But in developing lesson materials, they usually used the smart tools for resource sharing and communication in the instructional manner. In conclusion, the gap between their perception of smart tools and lesson materials, and the educational implications will be discussed.

• International Journal of Computer Science & Network Security
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• v.21 no.6
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• pp.1-6
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• 2021

Recently, pre-trained convolutional neural network CNNs have been widely used and applied for medical image classification. These models can utilised in three different ways, for feature extraction, to use the architecture of the pre-trained model and to train some layers while freezing others. In this study, the ResNet18 pre-trained CNNs model is used for feature extraction, followed by the support vector machine for multiple classes to classify medical images from multi-classes, which is used as the main classifier. Our proposed classification method was implemented on Kvasir and PH2 medical image datasets. The overall accuracy was 93.38% and 91.67% for Kvasir and PH2 datasets, respectively. The classification results and performance of our proposed method outperformed some of the related similar methods in this area of study.