• Title/Summary/Keyword: PNA

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Highly Sensitive PNA Array Platform Technology for Single Nucleotide Mismatch Discrimination

  • Choi, Jae-Jin;Jang, Min-Jeong;Kim, Ji-Hyun;Park, Hee-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.20 no.2
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    • pp.287-293
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    • 2010
  • Reliable discrimination of a single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. The newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. In addition we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. The PNA arrays had superior perfect match-to-mismatch signal ratios and sensitivities. The relative signal intensities of mismatch PNA probes ranged from 1.6% to 12.1% of the perfect-match PNA probes. These results demonstrated that the PNA arrays were 2.0 to 37.3 times more specific and about 10 times more sensitive than DNA arrays. The PNA array showed the same specificity and sensitivity after 12-month storage at room temperature.

Experimental Study on the Stress-Strain and Acid-Resistance of Pine Needle Ash Concrete (솔잎재 콘크리트의 응력-변형과 내산성에 관한 실험적 연구)

  • 성찬용
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.41 no.5
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    • pp.99-103
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    • 1999
  • This study examines the stress-strain and acid-resistance of pine needle ash (PNA) concrete. Materials used for this experiment are PNA , normal portland cement, natural fine and coarse aggregate. Test results show that the highest static modulus of elasticity are achieved by 5 % PNA filled PNA concrete. Acid-resistance of PNA concrete is increased with increase of the content of PNA. It is 1.29 times of the normal cement concrete for 5 % PNA filled PNA concrete and 2.57 times fo r15% PNA filled PNA concrete, based on the elased days for 25% mass loss of original mass immersed in the 5% H2SO4 solution. Accordingly, PNA concrete will greatly improve the properties of concrete.

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Experimental Study on the Elastic Properties and Acid Resistance of Pine Needle Ash Concrete (솔잎재 콘크리트의 탄성특성 및 내산성에 관한 실험적 연구)

  • 남기성;민정기;김영익;서대석;이전성;성찬용
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1999.10c
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    • pp.271-276
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    • 1999
  • This study is performed to evaluate an elastic properties and acid-resistance of concrete using pine needle ash(PNA). Materials used for this experiment are PNA , normal portland cement, natural fine and coarse aggregate. Test results show that the highest ultrasonic pulse velocity , dynamic and static modulus of elasticity is achieved by 5% PNA filled PNA concrete, which has showed similar with those of thei normal cement concrete. Acid-resistance of PNA concrete is increased with increase of the contnet of PNA , it is 1.29 times of the normal cement concrete by 5% PNA fille PNA concrete an d2.57 times by 15% PNA filled PNA concrete . Accordingly , PNA concrete wil greatly improve the properties of concrete.

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Design and Implementation of HomePNA 2.0 MAC Controller Circuit (HomePNA 2.0 MAC Controller 회로의 설계 및 구현)

  • Kim, Jong-Won;Kim, Dae-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.1A
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    • pp.1-10
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    • 2006
  • The Home Phoneline Networking Alliance(HomePNA) 2.0 technology can establish a home network using existing in-home phone lines, which provides a channel rate of 4-32 Mbps. HomePNA 2.0 Medium Access Control(MAC) protocol adopts an IEEE 802.3 Carrier Sense Multiple Access with Collision Detection(CSMA/CD) access method, Quality of Service(QoS) algorithm, and Distributed Fair Priority Queuing(DFPQ) collision resolution algorithm. In this paper, we describe some performance analysis results of HomePNA 2.0 MAC protocol and the requirements of HomePNA 2.0 MAC controller. Then, we propose the architecture of HomePNA 2.0 MAC controller circuit, show the simulation result of each block included in HomePNA 2.0 MAC controller, and present the HomePNA 2.0 transceiver chip that we have implemented.

Performance Analysis of HomePNA 2.0 MAC Protocol (HomePNA 2.0 MAC 프로토콜의 성능 분석)

  • Kim, Jong-Won;Kim, Dae-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.10A
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    • pp.877-885
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    • 2005
  • The Home Phoneline Networking Alliance (HomePNA) 2.0 technology can establish a home network using existing in-home phone lines, which provides a channel rate of 4-32 Mbps. HomePNA 2.0 Medium Access Control(MAC) protocol adopts an IEEE 802.3 Carrier Sense Multiple Access with Collision Detection (CSMA/CD) access method, Quality of Service(QoS) algorithm, and Distributed Fair Priority Queuing(DFPQ) collision resolution algorithm. In this paper, we propose some mathematical models about the important elements of HomePNA 2.0 MAC protocol performance, which are Saturation Throughput, Packet Delay and Packet Jitter. Then, we present an overall performance analysis of HomePNA 2.0 MAC protocol along with simulations.

A design of HomePNA2.0 PHY. (10Mbps급 HomePNA2.0 PHY. 회로 설계)

  • 박성희;구기종;김종원
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.12C
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    • pp.1282-1287
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    • 2002
  • In this Paper, we present the design of 10Mbps HomePNA(Home Phoneline Networking Alliance) PHY which is Home Network Technology using phone-line. It is connected with external interface through MII(Media Independent Interface) and AFE(Analog Front End) Interface. 10Mbps HomePNA PHY is composed with Management Block IEEE 802.3 CSMA/CD MAC(Media Access Control) Block Modulator block and Demodulator block. For their verification, we designed a prototype FPGA PCB board using XPC860T made in Motorola. We verified HomePNA frame data transmission using a driver program based Linux kernel. we verified rate negotiation by HomepNA 2.0 Link Layer Protocol.

Performance Evaluation of the HomePNA 3.0 Asynchronous MAC Mode with Collision Management Protocol (HomePNA 3.0 비동기 MAC 모드의 Collision Management Protocol 성능 분석)

  • 김희천;정민영;이태진
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.7A
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    • pp.727-734
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    • 2004
  • Collision Management Protocol (CMP) efficiently resolves collisions when data frames are transmitted in networks consisting of HomPNA 3.0 asynchronous MAC mode device with random access. Unlike Distributed Fair Priority Queueing (DFPQ) algorithm in HomePNA 2.0 or Binary Exponential Backoff (BEB) algorithm in IEEE 802.11, order of retransmission is decided according to Collision Signaling Sequence (CSS) values allocated to each device. Thus, CMP can minimize the number of mean collisions because order of retransmission is decided in a deterministic way. In this paper. we evaluate the saturation performance of CMP in HomePNA 3.0 using an analytic method.

Lectin Histochemistry on the Effects of Smoking on Glycoconjugates of Rat Sebaceous Glands (흡연이 흰쥐 복부 피지샘의 glycoconjugates에 미치는 영향에 대한 lectin 조직화학)

  • Jo, Un-Bock;Jeong, Gil-Nam
    • Journal of Life Science
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    • v.18 no.11
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    • pp.1543-1550
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    • 2008
  • We studied the effects of smoking, which is one of indoor-environmental pollutants and related to various cancers, on glycoconjugates of rat sebaceous glands with the lectin histochemistry. To investigate the effects of smoking on glycoconjugates, male Sprague-Dawley rats were exposed to tabacco smoke for 10 minutes per day in an inhalation chamber for 1, 2, 3, and 5 days with active and passive exposure. For the structure of sebaceous glands we used PAS reaction, and for the glycoconjugates binding pattern 9 biotinylated lectins (DBA, SBA, PNA, BSL-1, WGA, RCA-1, UEA-1, Con A, and LCA) were used. Some remarkable changes, such as the decrease in the size of sebaceous glandular acini, the destruction of upper portion of sebaceous glands, vacuolation of central portion of sebocytes, and the immature sebaceous glandular acini were seen in the smoke-exposed rats. In the control rats, basal cells were stained with BSL-1, PNA and WGA, but the stronger reaction was founded in BSL-1 binding. Also, sebocytes were stained with PNA, WGA, Con A, BSL-1 and SBA, but stronger reactions were founded in PNA and Con A stainings. Specific changes in the lectin binding patterns were also observed in the smoke-exposed rats. In the basal cells of exposed rats, PNA binding increased, BSL-1 decreased but returned to control level, and WGA disappeared. Plus, immature glandular acini, which were not found in the control rats, were stained PNA, Con A and BSL-1, but the stronger reaction were founded in PNA and Con A binding. In conclusion, it was assumed that the tabacco smoke seriously effected on the structure and glycoconjugates metabolism of sebaceous glands.

Design of Receiver Architecture for HomePNA 2.0 Modem (HomePNA 2.0 모뎀 수신부 설계)

  • Choi, Sung-Woo;Kim, Jong-Won
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.9A
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    • pp.991-997
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    • 2004
  • In this paper, we propose the architecture of modem receiver to fabricate HomePNA 2.0 chip. HomePNA suffers from inferior channel because of bridge tap, the effect of amateur HAM band and so on. To transfer data over such channel, HomePNA 2.0 uses training sequence to equalize channel and uses FD-QAM optionally as modulation method. So modem receiver demodulate QAM based signal and needs optimum architecture that fully uses these transmission feature. As a result of research, we define 2 mode function of modem receiver depending on TX/RX state. In this paper, particularly, we show the algorithm of equalizer, carrier phase recovery and frame synchromzationblock and propose architecture that improve the performance of channel equalization and is stable in operation. In the end, we estimate the performance of proposed HomePNA2.0 modem receiver over HomePNA TEST LOOP using SPW program.

Design and Simulation of HomePNA 2.0 MAC Controller Circuit

  • Kim, Jong-Won;Kim, Dae-Young
    • Proceedings of the Korea Society of Information Technology Applications Conference
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    • 2005.11a
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    • pp.179-182
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    • 2005
  • The Home Phoneline Networking Alliance (HomePNA) 2.0 technology can establish a home network using existing in-home phone lines, which provides a channel rate of 4 - 32 Mbps. HomePNA 2.0 Medium Access Control (MAC) protocol adopts an IEEE 802.3 Carrier Sense Multiple Access with Collision Detection (CSMA/CD) access method, a Quality of Service (QoS) algorithm, and a Distributed Fair Priority Queuing (DFPQ) collision resolution algorithm. In this paper, we analyze the HomePNA 2.0 MAC protocol and propose the architecture of HomePNA 2.0 MAC controller circuit. Then, we present the simulation result of each block included in the HomePNA 2.0 MAC controller.

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