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http://dx.doi.org/10.4014/jmb.0903.04018

Highly Sensitive PNA Array Platform Technology for Single Nucleotide Mismatch Discrimination  

Choi, Jae-Jin (Panagene Inc.)
Jang, Min-Jeong (Panagene Inc.)
Kim, Ji-Hyun (Panagene Inc.)
Park, Hee-Kyung (Panagene Inc.)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.2, 2010 , pp. 287-293 More about this Journal
Abstract
Reliable discrimination of a single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. The newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. In addition we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. The PNA arrays had superior perfect match-to-mismatch signal ratios and sensitivities. The relative signal intensities of mismatch PNA probes ranged from 1.6% to 12.1% of the perfect-match PNA probes. These results demonstrated that the PNA arrays were 2.0 to 37.3 times more specific and about 10 times more sensitive than DNA arrays. The PNA array showed the same specificity and sensitivity after 12-month storage at room temperature.
Keywords
Peptide nucleic acid (PNA); PNA array; single nucleotide mismatch;
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