• Journal of Microbiology
• /
• v.33 no.2
• /
• pp.142-145
• /
• 1995

The pumb gene encoding a basic amino acid transport protein in Neurospora crassa could be cloned by using a mutant strain defective in pmb gene as a host strain, using a negative selection on the media containing amino acid analogue canavanine. To select positive transformants of the genes for cloning, an auxotrophic marker (his-2) was added to a pmb mutant strain by mating ; a triple mutant (pmn : pmb : his-2) was constructued by crossing a strain defective in basic amino acid transport system (# 1683-bat um 535 "A") to a double mutant strain defective in neutral amino acid transport and histidine production (mitrol : his-2 "a"). Crossing was performed on synthetic crossing (SC) media containing histidine. The pmn : pmb and pmn :pmb : his-2 strains were selected among the progeny colonies from crosses on plates containing 5- .mu.g/ml para-fluoro-phenylalanine (PFPA), 200 .mu.g/ml canavanine, and 500 .mu.g/ml histidine. The selected colonies were cultured on minimal media with or without histidine for discarding pmn : pmb strain, because the pmn : pmb : his -2 strain grows only on histidine containing media. The pmn :pmb : his-2 strain selected can be used as a host strain for the cloning of the pmb and the pmn genes from a Neurospora genomic library by means of positive selections.

• Korean Journal of Poultry Science
• /
• v.34 no.4
• /
• pp.237-243
• /
• 2007

The objective of this experiment was to investigate the feeding influence of Prunus memu by-products (PMB) on productivity, blood composition, meat quality and intestinal microflora in semi-broiler chicks. Four hundred one day old chicks(Hanhyup 3) were raised in the floor pen with five treatments(0, 0.5, 1.0 2.0 3.0%) of four replicate for ten weeks. Basal diets contained 3,000, 3,100, 3,100, 3,200 kcal/kg ME and 22, 21, 19, 17% CP, respectively. There were no different performance for the first two weeks, but showed significantly different weight gain, feed intake in PMB 2.0, 3.0% addition treatments compared with control from three to five weeks of age. Weight gain of birds fed 2.0% PMB was significantly higher from nine to 10 weeks of age than control(P<0.05). Total weight gain tended to increase in PMB 1.0, 2.0, 3.0% addition treatments. Feed intake also showed increase in PMB 2.0, 3.0% addition treatments, but there were no different feed conversion. Total protein, albumin, cholesterol, neutral fat, glucose was significantly decreased in PMB 0.5% addition treatments compared with control. There were no different physico-chemical characteristics in breast meat. The number of yeast was significantly improved in PMB 3.0% compared with control(P<0.05), but found no difference in number of E. coli between control and PMB treatments. ND titer of birds fed PMB was not statistically different, but tended to decrease as dietary PMB increased. As the result of this experiment, PMB would be available as a feed additives in semi-broiler chicks.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2001.07a
• /
• pp.806-808
• /
• 2001

In this thesis, specimens was manufactured in general method annexing PMB-PZT system ceramic, and the following conclusion has been deduced. In XRD, the crystal structure of ceramic has the tetragonal structure which is close to rombohedral structure, and consequently the specimen characterized by MPB was manufactured. According to dopping with xPMB, electromechanical factor(kp) little is changed. kp was maximum value 23.37[%] at xPMB 0.03[mol%]. mechanical quality factor(Qm) was maximum value 237.04 at xPMB 0.03[mo1%].

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2002.07b
• /
• pp.578-580
• /
• 2002

In this thesis, specimens was manufactured in general method annexing PMB-PZT system ceramic, and the following conclusion has been deduced. In XRD, the crystal structure of ceramic has the tetragonal structure which is close to rombohedral structure, and consequently the specimen characterized by MPB was manufactured. According to dopping with xPMB, electromechanical factor(kp) little is changed. kp was maximum value 23.37[%] at xPMB 0.03[mol%]. mechanical quality factor(Qm) was maximum value 237,04 at xPMB 0.03[mol%].

• Proceedings of the KIEE Conference
• /
• 2001.07c
• /
• pp.1524-1526
• /
• 2001

In this thesis, specimens was manufactured in general method annexing PMB-PZT system ceramic, and the following conclusion has been deduced. In XRD, the crystal structure of ceramic has the tetragonal structure which is close to rombohedral structure, and consequently the specimen characterized by MPB was manufactured. According to dopping with xPMB, the dielectric constant at 20[$^{\circ}C$] reduced on the whole, dielectric constant was maximum value $8.0{\times}10^2$ at xPMB 0.01[mol%]. Dielectric loss was maximum value 4.1[%] at xPMB 0.01[mol%].

• Journal of the Korea Institute of Military Science and Technology
• /
• v.9 no.2 s.25
• /
• pp.60-69
• /
• 2006

The PMB(performance measurement baseline) is the total time-phased budget plan against which program performance is measured. Additionally it is the schedule for expenditure of the resources allocated to accomplish program scope and schedule objectives, and is formed by the budget assigned. In this paper, we suggest an improvement of the R & D(research and development) program for defense acquisition by using the PMB and an application of the IBR(integrated baseline review) to review the PMB for project manager of the defense agency.

• Biomaterials and Biomechanics in Bioengineering
• /
• v.1 no.2
• /
• pp.95-104
• /
• 2014

The objective of this study was to evaluate the cytotoxicity of poly (lactic acid) (PLA) nanoparticles. We used a water-soluble, amphiphilic phospholipid polymer, poly (2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB30W), as a stabilizer for the PLA nanoparticles. The PLA nanoparticles and PMB30W-modified PLA (PLA/PMB30W) nanoparticles were prepared by evaporating tetrahydrofuran (THF) from its aqueous solution. Precipitation of the polymers from the aqueous solution produced PLA and PLA/PMB30W nanoparticles with a size distribution of $0.4-0.5{\mu}m$. The partial coverage of PMB30W on the surface of the PLA/PMB30W nanoparticles was confirmed by X-ray photoelectron spectroscopy (XPS) and dynamic light-scattering (DLS). A high-content automated screening assay (240 random fields per group) revealed that the PLA nanoparticles induced apoptosis in a mouse macrophage-like cell line (apoptotic population: 73.9% in 0.8 mg PLA/mL), while the PLA/PMB30W nanoparticles remained relatively non-hazardous in vitro (apoptotic population: 13.8% in 0.8 mg PLA/mL). The reduction of the apoptotic population was attributed to the phosphorylcholine groups in the PMB30W bound to the surface of the nanoparticle. In conclusion, precipitation of PLA in THF aqueous solution enabled the preparation of PLA nanoparticles with similar shapes and size distribution but different surface characteristics. PMB30W was an effective stabilizer and surface modifier, which reduced the cytotoxicity of PLA nanoparticles by enabling their avoidance of the mononuclear phagocyte system.

• Food Science of Animal Resources
• /
• v.41 no.6
• /
• pp.905-922
• /
• 2021

Novel bioactive metabolites have been developed through a bioconversion of dairy products or other foods using probiotics isolated from dairy products or other fermented foods. These probiotics-mediated bioconversion (PMB) metabolites show antioxidant, anti-inflammatory, antimicrobial, epithelial barrier, and anticancer activities. In addition, the effect of PMB metabolites in periodontitis is recently reported in several studies. Periodontitis is a chronic inflammatory disease caused by infections, and the tooth support tissue is destroyed. Common treatments for periodontitis include scaling and root planning with systemic antibiotics. However, the overuse of antibiotics has led to the emergence of drug-resistant microorganisms and disturbs the beneficial bacteria, including lactobacilli in the oral cavity. For this reason, PMB metabolites, such as fermented milk, have been suggested as substitutes for antibiotics to reduce periodontitis. This paper reviews the recent studies on the correlation between periodontitis and PMB metabolites and classifies the efficacy of major PMB metabolites for periodontitis. The review suggests that PMB is effective for periodontitis, and further studies are needed to confirm the therapeutic effect of PMB metabolites on periodontitis.

• Annals of Geriatric Medicine and Research
• /
• v.22 no.4
• /
• pp.189-193
• /
• 2018

Background: This study aimed to reveal the clinicopathologic features and causes of bleeding in older patients with postmenopausal bleeding (PMB) and to investigate the correlation between the ultrasonographic findings and etiology of PMB. Methods: We retrospectively analyzed the causes and clinical characteristics of PMB in 498 patients who were diagnosed between January 2007 and December 2017. The population with PMB was divided into 2 groups according to age: Group A (n=204) included individuals more than 65 years of age and group B (n=294) included those less than 65 years of age. Clinical characteristics such as age, parity, underlying conditions, previous surgical history, and previous menopausal hormone therapy were compared between the groups. Cervical cytology testing and transvaginal ultrasonography were performed in all patients with PMB. Endometrial biopsy was performed in all cases of endometrial thickness ${\geq}5mm$. Results: We examined 498 patients with PMB. In group A, atrophic endometrium (n=125, 61.27%) was the most common cause of PMB. Twenty-three patients had gynecological malignancy (cervical cancer: n=12, 5.88%; endometrial cancer: n=8, 3.42%; ovarian cancer: n=3, 1.46%), and 30 patients had benign gynecological disease (endometrial polyp: n=10, 4.90%; submucosal myoma: n=6, 2.94%; uterine prolapse: n=7, 3.42%; cervical dysplasia; n=5, 2.45%; cervical polyp: n=2, 0.98%). Forty patients had endometrial thickness ${\geq}5mm$. Eight patients were diagnosed with endometrial cancer. All cases of endometrial cancer were diagnosed with endometrial thickness >10 mm. Conclusion: Atrophic endometrium was the most common cause of PMB in both groups, and approximately 12% of cases were associated with gynecological malignancy in older patients.

• Proceedings of the Korean Institute Of Construction Engineering and Management
• /
• 2008.11a
• /
• pp.513-516
• /
• 2008

The risk in the constructions projects is extremely diverse because there are too many indefinite factors. Therefore, Performance Measurement Baseline(PMB) is needed regularly during the construction process. However, nowdays, the PMB in the constructions projects is for contractors' PMB and Performance Management(PM). Clients have a lot of difficulties to control and manage the projects. Therefore, this paper classifies schedule and cost data of the public apartments houses which is a actual cases into types and then conducts regression analysis and deduces a regression. In addition to this paper establishes the Work Breakdown Structure(WBS) and suggests the PMB for the clients through using that.