• The Korean Journal of Physiology and Pharmacology
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• v.19 no.3
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• pp.283-289
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• 2015

This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy number significantly decreased and subsequently recovered in a dose-dependent manner. The respiratory control ratio to mitochondrial DNA copy number showed a dose-dependent increment. As times passed, Bax, caspase 9, PGC-$1{\alpha}$ and ${\beta}$-actin increased, and Bcl-2 decreased in a dose-dependent manner. However, the BMCF can be seen to have had no effect on Fas receptor. PINK1 expression did not change considerably and was inclined to decrease in control group, but the expression was down-regulated then subsequently increased with the use of the BMCF in a dose-dependent manner. Caspase 3 increased and subsequently decreased in a dose-dependent manner. These results suggest that the BMCF invigorates mitophagy and improves mitochondrial oxidative respiration in skeletal muscle, and in early stage of apoptosis induced by the BMCF is not related to extrinsic death-receptor mediated but mitochondria-mediated signaling pathway.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1977.06a
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• pp.7.1-7
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• 1977

According to Bryce and Cranston, prolapse of the laryngeal ventricle is a protrusion of the ventricular mucosa into the laryngeal cavity from inflammatory hyperplasia or edema. This 53 year old female patient was admitted to our hospital March 11, 1977 with the chief complaints of dyspnea and hoarseness. On admission, evaluation of the larynx revealed smooth surfaced round pink-reddish mass protruding bilaterally beneath the false cord. The airway was almost completely obstructed by the tumor mass, but neither ulceration nor infiltration was seen, also no lymphnode was palpable in the neck. Tracheotomy was performed for the relief of dyspnea and biopsy was done endoscopically. Histologic study showed chronic inflammation. On April 2, 1977 laryngofissure was performed under general anesthesia. Laryngeal examination revealed smooth surfaced, plate like, pinkreddish mass protruding bilaterally from both laryngeal ventricles into the laryngeal cavity and extending the whole length of the ventricle antero-posteriorly and touching each other. Biopsy was taken of the mass and the result was non-specific inflammatory hyperplasia of the larynx. Postoperatively, the air way is completely clear of obstruction but mild hoarseness still remains. No recurrent mass is visible in the larynx. In view of the symptoms, operative findings and histological findings, this case is diagnosed as "Prolapse of the laryngeal ventricle". The prolapse of the laryngeal ventricle is a very rare lesion and is reported with review of the literature.

• Journal of the Mineralogical Society of Korea
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• v.22 no.4
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• pp.385-393
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• 2009

The spectroscopic characteristics of Madagascar tourmaline were investigated by UV-Vis and FTIR spectroscopy. Physical features were similar to other region's tourmalines. The green and blue samples showed strong absorption band in the 714~743 nm due to $Fe^{3+}$, pink samples showed strong absorption band in the 510~530 nm due to $Mn^{3+}$, brown samples showed strong absorption at 324 nm due to $Mn^{2+}-Ti^{4+}$ IVCT and the colorless samples only revealed weak absorption at 406~413 nm or no absorptions due to low quantity of Mn. Combination of the stretching and bending mode cationic hydroxyl units (metal-OH) are observed in the 4300~4500 $cm^{-1}$. The parallel tube-shaped inclusions which contain hematite were detected generally. This investigation revealed that Cu was not detected but Fe and Mn were detected in the Madagascar tourmalines, and the various colors appear according to the amount of those impurity elements.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.1022-1027
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• 2004

The monacolin K content was determined to investigate the stability of monacolin K from red yeast rice after heating (20∼8$0^{\circ}C$), adjusting the pH (2∼8) by adding 3 N HCl or 3 N NaOH, adding the organic acid (6.0% acetic acid, 0.6% citric acid, 1.5% lactic acid) to pH 4.0 and adding the water (0∼80%). And the monacolin K was isolated from red yeast rice by conducting open column chromatography using neutral aluminum oxide. As a result, the stability of monacolin K decreased with increasing the temperature. The stability for pH was in the order of the unadjusted pH (pH 5.9)>8>4 and pH 2>10. The stability for organic acid was high in the order of lactic acid>citric acid>acetic acid, and the stability of monacolin K under acid was different according to the acid type. The degradation rate of monacolin K increased with increasing the water content. Moreover monacolin K was able to isolate from red and pink pigments as well as the other noncoloric compounds in red yeast rice. The yield of monacolin K was found to be 70%.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.280-283
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• 2010

A new standard rose cultivar 'Noble Red' was bred by the cross between the pink standard cultivar 'Silba 87' and the red standard cultivar 'Bravo' at the National Institute of Horticultural and Herbal Science. The cross was made in 1999 and 'Noble Red' was finally selected in 2003 after investigating the characteristics such as flower Color, Fragrance, stem length, stem diameter, weight, and number of leaves for three years from 2001 to 2003. 'Noble Red', a red standard cultivar grows vigorously and has good flower shape. The major characteristics of this cultivar are $125stems/m^2/year$ in yield, 71.9 cm in length of cut flower, 6.0cm in flower diameter, 30.8 in petal number, and 9.2 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cultivar, 'Rote Rose'.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.19 no.6
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• pp.37-48
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• 2020

Eight colors prescribed by the Enforcement Rules of the Road Traffic Act and the group standard were tested to secure the minimum recursive reflectance performance standards when drying and wetting. The results were calculated to be 260.8 (mcd/㎡·lux) when drying white and 154.6 (mcd/㎡·lux) when wet. Yellow was 67% compared to the white reflective performance when drying. Wet poetry was 79 % and 59 %, respectively. In the case of blue, it was 64% in the case of white versus 72% in the case of white. Wet poetry was 63 % and 72 %, respectively. The range of changes in reflective performance during wetting was higher than when drying, and the absence of glass grains was similar to the previous results. The new colors also have a standard value of more than 50% compared to the white color in red, orange, pink, light green, and green. Based on this, it was estimated that the minimum reflective performance criteria according to the color of the road markings would form the basis for the enforcement rules of the Road Traffic Act.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.1-8
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• 2010

Sensitivities of PLBs of four Phalaenopsis cultivars, P. 'Taisuco Windian', P. 'Nancy Amour', P. 'Pink Twilight' and P. 'Taipei Gold' to kanamycin, spectinomycin and hygromycin at different concentrations (0, 25, 50, 100, 200, and $400mg{\cdot}L^{-1}$) were examined. Hygromycin was favorable for selecting the transformants in the genetic transformation of Phalaenopsis as PLBs of four cultivars were all dead at even $25mg{\cdot}L^{-1}$ hygromycin. Responses of PLBs of P. 'Maki Watanabe' and P. 'Brother Lawrence' to DL-phosphinothricin (PPT) were determined at different concentrations (0, 0.1. 0.25, 0.5, 1.0, 2.0, 2.5, and $5.0mg{\cdot}L^{-1}$) and $0.5mg{\cdot}L^{-1}$ PPT was thought to be suitable for selecting the transformants of Phalaenopsis. The optimum conditions for Agrobacterium cocultivation with Phalaenopsis PLBs were examined using a two-step cocultivation method in Dtps. 'City Girl' and A. tumefaciens LBA4404. In the first infection period in a 1 : 10 suspension of Agrobacterium to a VW medium, 1 hr infection showed the highest PLB survival ratio. And then, PLBs were cocultivated with a bacterial strain and a 3-day cocultivation period was better for Phalaenopsis PLBs than a prolonged period. Agrobacterium tumefaciens strains LBA4404 (pTOK233) and EHA105 (pGA643) were used to compare their efficiency on the genetic transformation of Phalaenopsis PLBs. The PLBs infected with EHA105 survived more than those infected with LBA4404 after two days in a dark condition and two weeks in light condition on a selective medium. About 1,000 PLBs for each of P. 'Maki Watanabe' and P. 'Brother Lawrence', and each bacterial strain of AGL1 (pCAMBIA3301) and LBA4404 (pTOK233) were used for the regeneration of transgenic plants. The bacterial strain AGL1 had a higher genetic transformation efficiency than LBA4404, with no significant difference between cultivars. In this study, 11 hygromycin-resistant plantlets and 32 PPT-resistant plantlets were produced, but these putative transgenic plantlets need further examinations.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.29-34
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• 2009

The Chrysanthemum zawadskii complex is demarcated from other species by having the white and pink ligulate flowers. Its morphological characters are greatly diversified, so that various classification systems have been suggested. The character of leaf thickness has been mentioned as the characteristic for recognizing some of infra-specific taxa within this complex. In this study, we used longitudinal leaf sections to investigate the leaf thickness and cell number of leaf blades of 13 populations including those of the members of the C. zawadskii complex, as well as 4 populations of the related species of C. boreale and C. indicum. From the result, it was clear that the leaves were thicker in populations of C. boreale, C. indicum and C. zawadskii complex (diminishing in that order), and that the leaves were composed of about 9 cell layers in all populations. Within the C. zawadskii complex, leaf shape and thickness varied among the populations. It was very interesting that the taxa with restricted distribution, like C. zawadskii var. tenuisectum, C. zawadskii var. alpinum, C. zawadskii var. lucidum, and C. zawadskii subsp. coreanum had a thicker leaves than found among widely occurring taxa. From this, leaf thickness is supposed to be an adaptation to the unique habitat of each population.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.470-478
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• 2015

This study was investigated the anthocyanin composition of 'Gaeryangmeoru', 'Kyoho', and 'Hongisul' grapes cultivated in Korea using ultra-performance liquid chromatography (UPLC) coupled to a mass spectrometer (MS) equipped with an ESI (electrospray ionization) source. 'Gaeryangmeoru' is a dark-blue grape used for winemaking that can reach its coloring in unfavorable weather. The 'Kyoho' and 'Hongisul' varieties are hybrid grapes that feature black and pink skin, respectively. The anthocyanins extracted from the peels of grapes were analyzed using UPLC-ESI-MS/MS. Twenty-five anthocyanins were identified in the 'Gaeryangmeoru' and 'Kyoho' varieties, and 21 were identified in the 'Hongisul' variety. Eight, 14 and five predominant anthocyanins were identified in 'Gaeryangmeoru', 'Kyoho' and 'Hongisul' grape respectively. In all three varieties, mono-glucosides were 2.3-5.9 times more abundant than di-glucoside. Malvidin was the predominant anthocyanidin in 'Gaeryangmeoru' (44.1%) and 'Kyoho' (56.5%), but cyanidin (96.8%) was in 'Hongisul'. The acylated anthocyanins in 'Gaeryangmeoru' (2.0%) were rare, whereas acylated anthocyanins with p-coumaric acid were predominant in 'Kyoho' (40.9%) and 'Hongisul' (70.7%). In particular, cyanidin feruloyl glucoside was found only in the 'Hongisul' cultivar and considered to be useful as a criterion for identification of the variety. As a result, the grape varieties were demonstrated to have variety-specific anthocyanin characteristics, enabling classification based on anthocyanin composition in terms of anthocyanidins, glucosylation and acylation. The taxonomical application of anthocyanin composition confirmed the possibility that 'Gaeryangmeoru' originated from Vitis amurensis or its hybrids, and the 'Hongisul' grape was distinguished from other grapes by cyanidin feruloyl glucoside.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.375-380
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• 2003

This study was conducted to establish a practical masspropagation system of Phalaenopsis clones from basal shoot segments. The frequency of PLB (protocorm like body) induction was compared with various explants. Basal shoot segments showed the most successful result of 45％, while root tips, stalk node segments, stalk leaves and mature leaves represented low frequency (below 5％). The PLB induction ratio in the culture of basal shoot segments was examined with 11 different Phalaenopsis varieties, and the majority of varieties, including pink flower lines, showed an about 30％ rate of PLB formation. Especially, when whole basal shoot parts without cutting were inoculated onto PLB induction medium, giant PLB was induced from explant. This giant PLB was green color and big in size compared with normal PLB. When dissected giant PLB segments inoculated onto PLB multiplication medium, only normal size of PLBs were induced from them. PLBs induced by basal shoot culture were transferred onto proliferation medium and then shooting medium, from which normal plants were formed. Therefore, this culture method is considered as effective and practical protocol for Phalaenopsis mericlone production. In addition, it is suggested that clones of an infinite number can be produced consecutively by this culture system through repeated cycles of PLB induction and proliferation using the basal shoot segment of flask plant.