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http://dx.doi.org/10.5010/JPB.2003.30.4.375

Continuous Production of Phalaenopsis Clones by Basal Shoot Culture  

Been, Chul-Gu (Floricultural Experiment Station, Gyeongsangnam-do ARES)
Publication Information
Journal of Plant Biotechnology / v.30, no.4, 2003 , pp. 375-380 More about this Journal
Abstract
This study was conducted to establish a practical masspropagation system of Phalaenopsis clones from basal shoot segments. The frequency of PLB (protocorm like body) induction was compared with various explants. Basal shoot segments showed the most successful result of 45％, while root tips, stalk node segments, stalk leaves and mature leaves represented low frequency (below 5％). The PLB induction ratio in the culture of basal shoot segments was examined with 11 different Phalaenopsis varieties, and the majority of varieties, including pink flower lines, showed an about 30％ rate of PLB formation. Especially, when whole basal shoot parts without cutting were inoculated onto PLB induction medium, giant PLB was induced from explant. This giant PLB was green color and big in size compared with normal PLB. When dissected giant PLB segments inoculated onto PLB multiplication medium, only normal size of PLBs were induced from them. PLBs induced by basal shoot culture were transferred onto proliferation medium and then shooting medium, from which normal plants were formed. Therefore, this culture method is considered as effective and practical protocol for Phalaenopsis mericlone production. In addition, it is suggested that clones of an infinite number can be produced consecutively by this culture system through repeated cycles of PLB induction and proliferation using the basal shoot segment of flask plant.
Keywords
Doritaenopsis; PLB induction;
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