• 정보와 통신
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• 제32권10호
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• pp.11-17
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• 2015

네트워크 중심전에서 위성통신체계에 대한 중요성은 증가하고 있고, 차기 위성 통신 체계의 요구사항들을 만족하기 위해서는 PEP (Performance Enhancing Proxy) 기술의 적용이 필수적이다. 본 고에서는 차기 군 위성 통신 체계에서 적용 가능한 PEP 주요기술들과 상용 제품들의 특성을 알아보고, cross-layer 기반 PEP기술과 SOTM (Satellite communication On-The-Move) 단말을 위한 PEP 기술 동향에 대해서 살펴본다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.147.2-148
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• 2003

Complete nucleotide sequence of genome RNA of a Korean isolate of Pepper mottle virus (PepMoV-Vb) from field-collected diseased paprika (Capsicum annuum var grossum) was determined in this study. Symptoms of isolates of PepMoV were divided largely into two groups, vein banding (Vb) and vein clearing (Vc) patterns. PepMoV-Vb RNA consists of 9,640 nucleotides excluding the poly(A) tail. A single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3024 amino acids which is typical of the Potyvirus genus. The complete nucleotide sequence and coding regions of PepMoV-Vb were compared to that of 11 potyviruses within the genus Potyvirus. The overall nucleotide sequence identity was 94.7 and 94.1％ identical to PepMoV-C and PepMoV-FL, respectively. Full-length cDNAs of PepMoV-Vbl were synthesized from purified viral RNAs by RT-PCR and their genome structure was analysed by RFLP analysis. This is the first report on complete nucleotide sequence of PepMoV isolated from paprika in Korea.

• The Plant Pathology Journal
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• 제20권4호
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• pp.258-263
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• 2004

Ripe fruit of pepper (Capsicum annuum) showed resistance to Colletotrichum gloeoporioides, but unripe fruit was susceptible. We previously isolated the PepTLP gene that induced in both unripe and ripe fruit by fungal infection and wound, and only in ripe fruit by jasmonic acid (JA) treatment. To examine further regulation of PepTLP, the action of specific agonist and antagonists of known signaling effector on the .PepTLP expression by fungal infection, wound, and JA was investigated. A similar dephosphorylation event negatively activated all the PepTLP expression in the ripe fruit by fungal infection, wound, and JA. The induction of PepTLP expression by wound is differentially regulated via phosphorylation and dephosphorylation step during pre- and post-ripening, respectively. In addition, the induction of PepTLP expression in the ripe fruit by wound and JA is differentially regulated via dephosphorylation and phosphorylation step, respectively. Only both wound and JA treatment has synergistic effect on the PepTLP expression in the unripe fruit. Both SA and JA treatments on the unripe fruit, and both wound or JA and SA on the ripe fruit could not do any effect on the expression of PepTLP. These results suggest that the induction of PepTLP expression is differentially regulated via complex regulatory system against fungal infection, wound, and JA treatment during pre- and post-ripening of pepper fruit.

• BMB Reports
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• 제51권10호
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• pp.538-543
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• 2018

Pancreatic beta cell destruction and dysfunction induced by cytokines is a major cause of type 1 diabetes. Paraoxonase 1 (PON1), an arylesterase with antioxidant activity, has been shown to play an important role in preventing the development of diabetes in transgenic mice. However, no studies have examined the anti-diabetic effect of PON1 delivered to beta cells using protein transduction. In this study, we expressed the cell-permeable PON1 fused with PEP-1 protein transduction domain (PEP-1-PON1) to investigate whether transduced PEP-1-PON1 protects beta cells against cytokine-induced cytotoxicity. PEP-1-PON1 was effectively delivered to INS-1 cells and prevented cytokine-induced cell destruction in a dose-dependent manner. Transduced PEP-1-PON1 significantly reduced the levels of reactive oxygen species (ROS) and nitric oxide (NO), DNA fragmentation, and expression of inflammatory mediators, endoplasmic reticulum (ER) stress proteins, and apoptosis-related proteins in cytokine-treated cells. Moreover, transduced PEP-1-PON1 restored the decrease in basal and glucose-stimulated insulin secretion induced by cytokines. These data indicate that PEP-1-PON1 protects beta cells from cytokine-induced cytotoxicity by alleviating oxidative/nitrosative stress, ER stress, and inflammation. Thus, PEP-1-mediated PON1 transduction might be an effective method to reduce the extent of destruction and dysfunction of pancreatic beta cells in autoimmune diabetes.

• Journal of Communications and Networks
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• 제13권5호
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• pp.518-524
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• 2011

A high assurance IP encryption (HAIPE) compliant protocol accelerator is proposed for military networks consisting of red (or classified) networks and black (or unclassified) networks. The boundary between red and black sides is assumed to be protected via a HAIPE device. However, the IP layer encryption introduces challenges for bandwidth on demand satellite communication. The problems experienced by transmission control protocol (TCP) over satellites are well understood: While standard modems (on the black side) employ TCP performance enhancing proxy (PEP) which has been shown to work well, the HAIPE encryption of TCP headers renders the onboard modem's PEP ineffective. This is attributed to the fact that under the bandwidth-on-demand environment, PEP must use traditional TCP mechanisms such as slow start to probe for the available bandwidth of the link (which eliminates the usefulness of the PEP). Most implementations recommend disabling the PEP when a HAIPE device is used. In this paper, we propose a novel solution, namely broadband HAIPE-embeddable satellite communications terminal (BHeST), which utilizes dynamic network performance enhancement algorithms for high latency bandwidth-on-demand satellite links protected by HAIPE. By moving the PEP into the red network and exploiting the explicit congestion notification bypass mechanism allowed by the latest HAIPE standard, we have been able to regain PEP's desired network enhancement that was lost due to HAIPE encryption (even though the idea of deploying PEP at the modem side is not new). Our BHeST solution employs direct video broadcast-return channel service (DVB-RCS), an open standard as a means of providing bandwidth-on-demand satellite links. Another issue we address is the estimation of current satellite bandwidth allocated to a remote terminal which is not available in DVBRCS. Simulation results show that the improvement of our solution over FIX PEP is significant and could reach up to 100%. The improvement over the original TCP is even more (up to 500% for certain configurations).

• Journal of Ginseng Research
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• 제43권2호
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• pp.218-225
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• 2019

Background: Streptococcus pneumoniae, more than 90 serotypes of which exist, is recognized as an etiologic agent of pneumonia, meningitis, and sepsis associated with significant morbidity and mortality worldwide. Immunization with a pneumococcal pep27 mutant (${{\Delta}}pep27$) has been shown to confer comprehensive, long-term protection against even nontypeable strains. However, ${{\Delta}}pep27$ is effective as a vaccine only after at least three rounds of immunization. Therefore, treatments capable of enhancing the efficiency of ${{\Delta}}pep27$ immunization should be identified without delay. Panax ginseng Mayer has already been shown to have pharmacological and antioxidant effects. Here, the ability of Korean Red Ginseng (KRG) to enhance the efficacy of ${{\Delta}}pep27$ immunization was investigated. Methods: Mice were treated with KRG and immunized with ${{\Delta}}pep27$ before infection with the pathogenic S. pneumoniae strain D39. Total reactive oxygen species production was measured using lung homogenates, and inducible nitric oxide (NO) synthase and antiapoptotic protein expression was determined by immunoblotting. The phagocytic activity of peritoneal macrophages was also tested after KRG treatment. Results: Compared with the other treatments, KRG significantly increased survival rate after lethal challenge and resulted in faster bacterial clearance via increased phagocytosis. Moreover, KRG enhanced ${{\Delta}}pep27$ vaccine efficacy by inhibiting reactive oxygen species production, reducing extracellular signal-regulated kinase apoptosis signaling and inflammation. Conclusion: Taken together, our results suggest that KRG reduces the time required for immunization with the ${{\Delta}}pep27$ vaccine by enhancing its efficacy.

• Journal of Communications and Networks
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• 제16권1호
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• pp.26-35
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• 2014

We consider multiple-input multiple-output decode-and-forward relay systems in Rayleigh fading channels under the partial channel state information (CSI) that the channel statistics of the source-relay (SR) link and the instantaneous CSI of the source-destination and relay-destination links are known at the destination. In this paper, we propose a new near maximum likelihood (near-ML) decoder with two-level pairwise error probability (near-ML-2PEP) which uses the average PEP instead of the exact PEP. Then, we theoretically prove that the near-ML and near-ML-2PEP decoders achieve the maximum diversity, which is confirmed by Monte Carlo simulations. Moreover, we show that the near-ML-2PEP decoder can also achieve the maximum diversity by substituting the average PEP with the values that represent the error performance of the SR link.

• The Plant Pathology Journal
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• 제25권4호
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• pp.417-421
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• 2009

The analysis of the full length genome of Korean isolates of Pepper mottle virus (PepMoV) in previous study showed molecular variations and are found to be related to symptom variation and pathogenicity (Kim et al., 2009, Virus Res. 144:83-88). To fully understand the molecular variation of PepMoV in Korea, we further assessed the role of RNA recombination to biological variation and evolution of PepMoV. Full-length genome of a total of 17 Korean-PepMoV and 2 American (CA and FL) isolates were examined for possible detection of genetic recombination using different recombination detections programs and detected 5 and 8 tentative recombination events using RDP3 and Splits Tree4 programs, respectively. Interestingly, tentative recombinants detected such as isolates 57, 134 and 217 were previously identified as severe isolates and 205135 and 205136 as differentiating isolates (Kim et al., 2009, Virus Res. 144:83-88). In addition, recombination was frequently detected in the Vb isolate, the first PepMoV isolate reported in Korea, suggesting significant involvement in the evolution of PepMoV in Korea. These initial results of our recombination analyses among PepMoV isolates in Korea may serve as clues to further investigate the biological variations and evolution of PepMoV brought about by recombination.

• 전자공학회논문지
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• 제52권8호
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• pp.10-17
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• 2015

위성 통신 시스템은 광범위한 영역에서 TCP/IP를 통해 실행되는 인터넷 애플리케이션에 대한 합리적인 해결책중 하나이다. 그러나, 위성 통신에서의 긴 왕복전송 시간(RTT)과 높은 비트 에러율(BER)로 인해 TCP프로토콜은 심각한 성능 저하가 발생한다. 그러므로 위성 링크에 PEP를 적용하여 위성 환경에서의 TCP의 성능을 향상시킨다. 본 논문에서는 PEP 테스트베드를 구현하고, 다양한 환경에서 무선 채널을 고려한 변종 고속 TCP기법과 TCP연결 분할의 성능을 비교 평가하는 실험을 다중연결, 높은 패킷 손실 그리고 제한된 대역폭에서 수행하였다. 성능 분석 결과 PEP는 다양한 환경에서 변종 고속 TCP보다 TCP 처리율을 더 많이 향상할 수 있음을 확인하였다. 하지만 네트워크 혼잡이 포함된 환경에서는 다른 변종 TCP와 비슷한 성능을 나타냈다.

• International Journal of Oral Biology
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• 제39권4호
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• pp.169-176
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• 2014

A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format $O_{(1-6)}XXXXX-NH_2$, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-$NH_2$ (pep-1) and WXXXXN-$NH_2$ (pep-2), and one positioned peptide, RRRWRN-$NH_2$ (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic ${\alpha}$-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.