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http://dx.doi.org/10.5423/PPJ.2009.25.4.417

Effects of Recombination on the Pathogenicity and Evolution of Pepper mottle virus  

Jonson, Miranda Gilda (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University)
Seo, Jang-Kyun (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University)
Cho, Hong-Soo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Jeong-Soo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Kook-Hyung (Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University)
Publication Information
The Plant Pathology Journal / v.25, no.4, 2009 , pp. 417-421 More about this Journal
Abstract
The analysis of the full length genome of Korean isolates of Pepper mottle virus (PepMoV) in previous study showed molecular variations and are found to be related to symptom variation and pathogenicity (Kim et al., 2009, Virus Res. 144:83-88). To fully understand the molecular variation of PepMoV in Korea, we further assessed the role of RNA recombination to biological variation and evolution of PepMoV. Full-length genome of a total of 17 Korean-PepMoV and 2 American (CA and FL) isolates were examined for possible detection of genetic recombination using different recombination detections programs and detected 5 and 8 tentative recombination events using RDP3 and Splits Tree4 programs, respectively. Interestingly, tentative recombinants detected such as isolates 57, 134 and 217 were previously identified as severe isolates and 205135 and 205136 as differentiating isolates (Kim et al., 2009, Virus Res. 144:83-88). In addition, recombination was frequently detected in the Vb isolate, the first PepMoV isolate reported in Korea, suggesting significant involvement in the evolution of PepMoV in Korea. These initial results of our recombination analyses among PepMoV isolates in Korea may serve as clues to further investigate the biological variations and evolution of PepMoV brought about by recombination.
Keywords
genetic structure; molecular and biological variation; PepMoV; recombination;
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1 Fargette, D., Pinel, A., Traore, O., Ghesquiere, A. and Konate, G. 2002. Emergence of resistance-breaking isolates of Rice yellow mottle virus during serial inoculations. Eur. J. Plant Pathol. 108:585-591   DOI   ScienceOn
2 Huson, D. H. and Bryant, D. 2006. Application of phylogenetic networks in evolutionary studies. Mol. BioI. Evol. 23:254-267   DOI   ScienceOn
3 Jenner, C. E., Sanchez, F., Nettleship S. B., Foster G. D., Ponz, F. and Walsh J. A. 2000. The cylindrical inclusion gene of Turnip mosaic virus encodes a pathogenic determinant to the brassica resistance Gene TuRB01. Mol. Plant-Microbe Interact. 13:1102-1108   DOI   ScienceOn
4 Posada, D. and Crandall, K. 2001. Evaluation of methods for detecting recombination from DNA sequences: Computer simulations. Proc. Natl. Acad. Sci. USA 98:13757-13762   DOI   ScienceOn
5 Harrison, B. D. 2002. Virus variation in relation to resistance-breaking in plants. Euphytica 124:181-192   DOI   ScienceOn
6 Martin, D. P., Williamson, C. and Posada, D. 2005. RDP2: Recombination detection and analysis from sequence alignments. Bioinformatics 21:260-262   DOI   ScienceOn
7 Shukla, D. D., Ward, C. W. and Brunt, A. A. 1994. The Potyviridae. CAB international, 17 Wallingford, UK
8 Choi, B. K., Koo, J. M., Ahn, H. J., Yum, H. J., Choi, C. W., Ryu, K. H., Chen, P. and Tolin, S. A. 2005. Emergence of Rsv-resistance breaking Soybean mosaic virus isolates from Korean soybean cultivars. Virus Res. 112:42-51   DOI   ScienceOn
9 Etherington, G. J., Dicks, J. and Roberts, I. N. 2005. Recombination Analysis Tool (RAT): A program for the high-throughput detection of recombination. Bioinformatics 21:278-281   DOI   ScienceOn
10 Wylie, S. J. and Jones, R. A. C. 2009. Role of recombination in the evolution of host specialization within Bean yellow mosaic virus. Phytopathology 99:512-518   DOI   ScienceOn
11 Seo, J.-K. Ohshima, K., Lee, H.-G, Son, M.-I., Choi, H.-S., Lee, S.-H., Sohn, S.-H. and Kim, K-H. 2009b. Molecular variability and genetic structure of the population of Soybean mosaic virus based on the analysis of complete genome sequences. Virology 393:91-103   DOI   ScienceOn
12 Gagarinova, A. G., Babu, M., Stromvik, M. V. and Wang, A. 2008. Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes. Viral. J. 5:143   DOI   ScienceOn
13 Hajimorad, M. R, Eggenberger, A. L. and Hill, J. H. 2003. Evolution of Soybean mosaic virus-G7 molecularly cloned genome in Rsv1-genotype soybean results in emergence of a mutant capable of evading Rsv1-mediated recognition. Virology 314:497-509   DOI   ScienceOn
14 Kim, Y.-J., Jonson, M. G., Choi, H.-S., Ko, S.-J. and Kim, K.-H. 2009. Molecular characterization of Korean Pepper mottle virus isolates and its relationship to symptom variations. Virus Res. 144:83-88   DOI   ScienceOn
15 Roossinck, M. J. 1997. Mechanisms of plant virus evolution. Annu. Rev. Phytopathol. 35:191-209   DOI   PUBMED   ScienceOn
16 Verhoeven, J. Th., Willemen, T. M. and Roenhorst, J. W. 2002. First report of Pepper mottle virus in tomato. Plant Dis. 86:186   DOI
17 Vance, V. B., Moore, D., Turpen, T. H., Bracker, A. and Hollowell, V. C. 1992. The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191:19-30   DOI   ScienceOn
18 Worobey, M. and Holmes, E. C. 1999. Evolutionary aspects of recombination in RNA viruses. J. Gen. Viral. 80:2535-2543   DOI
19 Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. and Higgins, D. G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-2948   DOI   ScienceOn
20 Seo, J.-K., Lee, S.-H. and Kim, K.-H. 2009a. Strain-specific cylindrical inclusion protein of Soybean mosaic virus elicits extreme resistance and a lethal systemic hypersensitive response in two resistant soybean cultivars. Mol. Plant-Microbe Interact. 22:1151-1159   DOI   ScienceOn
21 Garcla-Arenal, F., Fraile, A. and Malpica, J. M. 2003. Variation and evolution of plant virus populations. Int. Microbiol. 6:225-232   DOI   ScienceOn
22 Weiller, G. F. 1998. Phylogenetic profiles: A graphical method for detecting genetic recombinations in homologous sequences. Mol. BioI. Evol. 15:326-335   DOI   ScienceOn
23 Kim, M.-K. Kwak, H.-R., Han, J.-H., Ko, S.-J., Lee, S.-H., Park, J.-W., Jonson, M.-G., Kim, K-H., Kim, J.-S., Choi, H.-S. and Cha, B.-J. 2008. Isolation and characterization of Pepper mottle virus infecting tomato in Korea. Plant Pathol. J. 24:152-158   DOI   ScienceOn