• 제목/요약/키워드: PASA

검색결과 17건 처리시간 0.017초

Evaluation of the radiopacity of restorative materials with different structures and thicknesses using a digital radiography system

  • Yaylaci, Ayla;Karaarslan, Emine Sirin;Hatırli, Huseyin
    • Imaging Science in Dentistry
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    • 제51권3호
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    • pp.261-269
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    • 2021
  • Purpose: The aim of this study was to evaluate the radiopacities of various types of restorative materials with different thicknesses compared with enamel, dentin, and aluminum. Materials and Methods: Four bulk-fill resins, 2 hybrid ceramics, 2 micro-hybrid resin composites, 6 glass ionomer-based materials, 2 zinc phosphate cements, and an amalgam were used in the study. Twelve disk-shaped specimens were prepared from each of 17 restorative materials with thicknesses of 1 mm, 2 mm, and 4 mm (n=4). All the restorative material specimens with the same thickness, an aluminum (Al) step wedge, and enamel and dentin specimens were positioned on a phosphor storage plate and exposed using a dental X-ray unit. The mean gray values were measured on digital images and converted to equivalent Al thicknesses. Statistical analyses were performed using 2-way analysis of variance and the Bonferroni post hoc test(P<0.05). Results: Radiopacity was significantly affected by both the thickness and the material type (P<0.05). GCP Glass Fill had the lowest radiopacity value for samples of 1 mm thickness, while Vita Enamic had the lowest radiopacity value for 2-mm-thick and 4-mm-thick samples. The materials with the highest radiopacity values after the amalgam were zinc phosphate cements. Conclusion: Significant differences were observed in the radiopacities of restorative materials with different thicknesses. Radiopacity was affected by both the material type and thickness.

분자학적 진단방법을 이용한 bifenazate 저항성 점박이응애 모니터링 (Monitoring of Bifenazate Resistant Two-spotted Spider Mite, Tetranychus urticae Using Molecular Detection Method)

  • 이규리;신윤호;조선란;구현나;최장전;안기수;김길하
    • 농약과학회지
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    • 제15권1호
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    • pp.61-67
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    • 2011
  • 2010년에 청주, 충주-1, 충주-2, 강진, 예산, 그리고 영주에서 채집한 점박이응애의 bifenazate 약제 저항성을 확인 해 본 결과, 강진과 예산 개체군에서 각각 964.5배, 1130배의 높은 저항성을 나타내었다. 그리고 청주, 충주-1, 충주-2, 그리고 영주개제군에서는 낮은 저항성비를 나타내었다. Bifenazate 약제 저항성 점박이응애의 cytb 점 돌연변이인 G126S를 확인해 본 결과, 생물검정에서 높은 저항성을 보인 강진과 예산 개체군에서 G126S 점 돌연변이를 확인 할 수 있었다. 이와 같이, G126S 점 돌연변이는 bifenazate 약제 저항성을 가지는 점박이응애 선별에 아주 유용한 분자진단 마커로 이용될 수 있다. 두 가지 분자진단 방법인 quantitative sequencing(QS)와 PCR amplification of specific alleles(PASA)는 G126S 점 돌연변이를 잘 탐지하였다. 따라서 이러한 방법들은 야외 계통의 bifenazate 약제 저항성 형질 모니터링과 저항성 관리에 효율적으로 이용될 수 있을 것이다.

Detection of Single Nucleotide Polymorphism in Human IL-4 Receptor by PCR Amplification of Specific Alleles

  • Hwang, Sue Yun;Kim, Seung Hoon;Hwang, Sung Hee;Cho, Chul Soo;Kim, Ho Youn
    • Animal cells and systems
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    • 제5권2호
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    • pp.153-156
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    • 2001
  • A key aspect of genomic research in the “post-genome era”is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large-scale association studies of genetic diseases, detection of SNPs from an extended number of human DNA samples is often difficult, labor-intensive and time-consuming. Recent development in SNP detection methods using DNA microarrays and mass spectrophotometry has allowed automated high throughput analyses, but such equipments are not accessible to many scientists. In this study, we demonstrate that a simple PCR-based method using primers with a mismatched base at the 3'-end provides a fast and easy tool to identify known SNPs from human genomic DNA in a regular molecular biology laboratory. Results from this PCR amplification of specific alleles (PASA) analysis efficiently and accurately typed the Q576R polymorphism of human IL4 receptor from the genomic DNAs of 29 Koreans, including 9 samples whose genotype could not be discerned by the conventiona1 PCR-SSCP (single strand conformation polymorphism) method. Given the increasing attention to disease-associated polymorphisms in genomic research, this alternative technique will be very useful to identify SNPs in large-scale population studies.

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TRANSLATION SURFACES OF TYPE 2 IN THE THREE DIMENSIONAL SIMPLY ISOTROPIC SPACE 𝕀13

  • Bukcu, Bahaddin;Karacan, Murat Kemal;Yoon, Dae Won
    • 대한수학회보
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    • 제54권3호
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    • pp.953-965
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    • 2017
  • In this paper, we classify translation surfaces of Type 2 in the three dimensional simply isotropic space ${\mathbb{I}}_3^1$ satisfying some algebraic equations in terms of the coordinate functions and the Laplacian operators with respect to the first, the second and the third fundamental form of the surface. We also give explicit forms of these surfaces.

피라세탐의 방출조절 및 생체이용률 (Controlled Release and Bioavailability of Piracetam)

  • 강진양;이경태;서성훈
    • Journal of Pharmaceutical Investigation
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    • 제28권2호
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    • pp.109-113
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    • 1998
  • This study is purposed to develop the sustained release and bioavailability of piracetam (PA). The use of alginate beads as a means to achieve sustained release of piracetam was evaluated in comparison with that of piracetam alone. In the PA-sodium alginate(SA) beads was confirmed by differential scanning calorimetry thermogram(DSC), indicating a relative shift of an endometric peak of PA to higher temperature. The changes in dissolution rates from PA-SA beads and PASA beads coated by chitosan(CHO) were significantly slower than that of intact PA. The release rate of PA-SA in the gastric fluid was markedly decreased compared with that in the intestinal fluid, suggesting that PA is mostly released in the intestinal fluid. However, the PA/SA ratio scarcely affected the release profile. The blood concentration- time curves of PA, PA-SA and PA-SA-CHO were obtained by oral administration to rats. $T_{max}$ of PA, PA-SA and PA-SA-CHO were 1, 10 and 6 hours, respectively. It was confirmed that the release of PA was prolonged by the formulation of PA-SA beads and PA-SA-CHO beads.

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