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Monitoring of Bifenazate Resistant Two-spotted Spider Mite, Tetranychus urticae Using Molecular Detection Method  

Lee, Kyu-Ri (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Shin, Yun-Ho (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Cho, Sun-Ran (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Koo, Hyun-Na (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Choi, Jang-Jeon (Pear Experiment Station, NHRI)
Ahn, Ki-Su (Chungbuk Provincial Agricultural Research & Extension Services)
Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.15, no.1, 2011 , pp. 61-67 More about this Journal
Abstract
In 2010, two-spotted spider mite, Tetranychus urticae was collected from the rose greenhouse and apple orchards in Cheongju (CJ), Chungju (CUJ)-1, CUJ-2, Kangjin (KJ), Yesan (YS), and Yeongju (YJ). Among them, KJ and YS strain showed high resistance to bifenazate of 964.5- and 1l30-fold, respectively. The other strains showed low resistance to bifenazate. By analyzing the mitochondrial cytochrome b (cytb) sequence, G126S point mutation was detected in KJ and YS strain. Thus, G126S point mutation in the mitochondrial cytb was available molecular detection marker for selection of bifenazate resistant T. urticae. Two molecular detection methods, quantitative sequencing (QS) and PCR amplification of specific alleles (PASA) were well detected specific G126S point mutation. Therefore, these methods can be used to monitor the resistance allele in field population of T. urticae and bifenazate resistance management strategy.
Keywords
Tetranychus urticae; Bifenazate; Point mutation; Cytochrome b; QS; PASA;
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