The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Monitoring of Bifenazate Resistant Two-spotted Spider Mite, Tetranychus urticae Using Molecular Detection Method

분자학적 진단방법을 이용한 bifenazate 저항성 점박이응애 모니터링

  • Lee, Kyu-Ri (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Shin, Yun-Ho (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Cho, Sun-Ran (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Koo, Hyun-Na (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Choi, Jang-Jeon (Pear Experiment Station, NHRI) ;
  • Ahn, Ki-Su (Chungbuk Provincial Agricultural Research & Extension Services) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
  • 이규리 (충북대학교 농업생명환경대학 식물의학과) ;
  • 신윤호 (충북대학교 농업생명환경대학 식물의학과) ;
  • 조선란 (충북대학교 농업생명환경대학 식물의학과) ;
  • 구현나 (충북대학교 농업생명환경대학 식물의학과) ;
  • 최장전 (국립원예특작과학원 배시험장) ;
  • 안기수 (충북농업기술원 친환경농업연구과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2011.02.14
  • Accepted : 2011.03.01
  • Published : 2011.03.31
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Abstract

In 2010, two-spotted spider mite, Tetranychus urticae was collected from the rose greenhouse and apple orchards in Cheongju (CJ), Chungju (CUJ)-1, CUJ-2, Kangjin (KJ), Yesan (YS), and Yeongju (YJ). Among them, KJ and YS strain showed high resistance to bifenazate of 964.5- and 1l30-fold, respectively. The other strains showed low resistance to bifenazate. By analyzing the mitochondrial cytochrome b (cytb) sequence, G126S point mutation was detected in KJ and YS strain. Thus, G126S point mutation in the mitochondrial cytb was available molecular detection marker for selection of bifenazate resistant T. urticae. Two molecular detection methods, quantitative sequencing (QS) and PCR amplification of specific alleles (PASA) were well detected specific G126S point mutation. Therefore, these methods can be used to monitor the resistance allele in field population of T. urticae and bifenazate resistance management strategy.

2010년에 청주, 충주-1, 충주-2, 강진, 예산, 그리고 영주에서 채집한 점박이응애의 bifenazate 약제 저항성을 확인 해 본 결과, 강진과 예산 개체군에서 각각 964.5배, 1130배의 높은 저항성을 나타내었다. 그리고 청주, 충주-1, 충주-2, 그리고 영주개제군에서는 낮은 저항성비를 나타내었다. Bifenazate 약제 저항성 점박이응애의 cytb 점 돌연변이인 G126S를 확인해 본 결과, 생물검정에서 높은 저항성을 보인 강진과 예산 개체군에서 G126S 점 돌연변이를 확인 할 수 있었다. 이와 같이, G126S 점 돌연변이는 bifenazate 약제 저항성을 가지는 점박이응애 선별에 아주 유용한 분자진단 마커로 이용될 수 있다. 두 가지 분자진단 방법인 quantitative sequencing(QS)와 PCR amplification of specific alleles(PASA)는 G126S 점 돌연변이를 잘 탐지하였다. 따라서 이러한 방법들은 야외 계통의 bifenazate 약제 저항성 형질 모니터링과 저항성 관리에 효율적으로 이용될 수 있을 것이다.

Keywords

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