• The Journal of Natural Sciences
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• v.14 no.1
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• pp.25-37
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• 2004

T7 RNA polymerase also recognize intrinsic, hairpin-independent termination signal, a conserved 7-base pair sequence (ATCTGTT in the non-template stand) and U-rich sequence downstream of it. These intrinsic, hairpin-independent termination signal were commonly found in PTH and CJ termination sequences. There are two types of mutant T7 RNA polymerases recognizing sensitively(X3, X19, BG8) of insensitively (R173C) the intrinsic termination signals. We determined the T7 transcription activities of these mutants. Compared to wild-type, mutants X4, 19 and BG8 show highly reduced transcription activities (8%, 33%, 34%). On the other hand mutant R173C shows comparable transcription activity of wild-type (112%). Also transcription termination efficiencies at the PTH or CJ termination signals were determined by using mutant RNA polymerases. Temination of mutnats X4, X19 and BG8 are increased compared to wild-type. On the other hadn mutant R173C proceeds through PTH and CJ termination signals.

• Elastomers and Composites
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• v.37 no.1
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• pp.21-30
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• 2002

Core-shell polymers of methyl methacrylate/styrene pair were prepared by sequential emulsion polymerization in the presence of sodium dodecyl benzene sulfonate(SDBS) as an emulsifier using ammonium persulfate(APS) as an initiator. The characteristics of these core-shell polymers were evaluated. Core-shell composite latex has the both properties of core and shell components in a particle, where as polymer blonds or copolymers show a combined properties from the physical properties or two homopolymers. This unique behavior of core-shell composite latex can be used in many industrial fields. However, in preparation of core-shell composite latex, several unexpected phenomina are observed, such as, particle coagulation, low degree of polymerization, and formation of new particles during shell polymerization. To solve the disadvantages, we studied the effects of surfactant concentrations, initiator concentrations, and reaction temperature on the tore-shell structure or PMMA/PSt and PSt/PMMA. Particle size and particle size distribution were measured by using particle size analyzer, and the morphology of the core-shell composite latex was observed by using transmission electron microscope. Glass transition temperature($T_g$) was also measured by using differential scanning calorimeter. To identify the core-shell structure, pH of the composite latex solutions were measured.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.12
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• pp.9-16
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• 2005

A new online routing algerian is proposed in this paper, which use the interference-prediction to solve the network congestion originated from extension of Internet scope and increasing amount of traffic. The end-to-end QoS has to be guaranteed in order to satisfy service level agreements (SLAs) in the integrated networks of next generation. For this purpose, bandwidth is allocated dynamically and effectively, moreover the path selection algorithm is required while considering the network performance. The proposed algorithm predicts the level of how much the amount of current demand interferes the future potential traffic, and then minimizes it. The proposed algorithm considers the bandwidth on demand, link state, and the information about ingress-egress pairs to maximize the network performance and to prevent the waste of the limited resources. In addition, the interference-prediction supports the bandwidth guarantee in dynamic network to accept more requests. In the result, the proposed algorithm performs the effective admission control and QoS routing. In this paper, we analyze the required conditions of routing algorithms, the aspect of recent research, and the representative algorithms to propose the optimized path selection algorithm adequate to Internet franc engineering. Based on these results, we analyze the problems of existing algorithms and propose our algorithm. The simulation shows improved performance by comparing with other algorithms and analyzing them.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.6
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• pp.167-176
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• 2005

This paper describes how the state tying model based on the decision tree which is one of Acoustic models used for speech recognition optimizes the model by reducing the number of mixture Gaussians of the output probability distribution. The state tying modeling uses a finite set of questions which is possible to include the phonological knowledge and the likelihood based decision criteria. And the recognition rate can be improved by increasing the number of mixture Gaussians of the output probability distribution. In this paper, we'll reduce the number of mixture Gaussians at the highest point of recognition rate by clustering the Gaussians. Bhattacharyya and Euclidean method will be used for the distance measure needed when clustering. And after calculating the mean and variance between the pair of lowest distance, the new Gaussians are created. The parameters for the new Gaussians are derived from the parameters of the Gaussians from which it is born. Experiments have been performed using the STOCKNAME (1,680) databases. And the test results show that the proposed method using Bhattacharyya distance measure maintains their recognition rate at $97.2\%$ and reduces the ratio of the number of mixture Gaussians by $1.0\%$. And the method using Euclidean distance measure shows that it maintains the recognition rate at $96.9\%$ and reduces the ratio of the number of mixture Gaussians by $1.0\%$. Then the methods can optimize the state tying model.

• Journal of Acupuncture Research
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• v.17 no.2
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• pp.231-246
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• 2000

Acupuncture has been used for treatment of numerous diseases, especially for pain control in the oriental culture. However, the mechanism of pain control by acupuncture was not clear so far. The present study was examined that the effects of electro-acupuncture (EA) applied to the acu-point of extra-segmental area on modulation of formalin induced pain in Sprague - Dawley rats. In order to apply EA to acu-points in the plantar area of right fore paws, a pair of teflon - coated stainless steel wires were implanted in HT 7 (shin-mun) and PC 7 (dae-neong) 5 days before behavioral test. A behavioral test was performed by means of video camera after injection of 5% formalin ($50{\mu}l$) into the lateral plantar region of left hind paw. EA was delivered by a constant current stimulator at 4~5 mA, 2 ms, and 10 Hz for 30 min. The electromyographic activities were recorded in the biceps femoris muscle under chloral hydrate anesthesia. Test stimuli with 1~9mA were applied to the sural nerve territory including the medial portion of the 4th toe and the lateral portion of the 5th toe. Behavioral responses including favoring, flinching and bitting were occured in the biphasic pattern, such as the lst phase (0~5 min) and the 2nd phase (20~45 min) after formalin injection. However, EA (4~5 mA, 2 ms, 10 Hz) significantly inhibited Che behavioral responses. EMG activities of flexor reflex had a latency of 100~300 ms and thresholds of test stimuli for EMG were 4~5 mA in normal rats. Injection of formalin decreased threshold of test stimuli and increased EMG activities for 2hrs after injection. However, EA significantly inhibited EMG activities of flexor reflex increased by formalin and recovered EMG evoked thresholds. These results suggest that contralateral extra-segmental EA inhibits the first and second phases of formalin induced pain but their mechanism be needed to examine additionally.

• Journal of Digital Convergence
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• v.13 no.5
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• pp.337-344
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• 2015

Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on $^1H-NMR$, $^{13}C-NMR$ spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration ($IC_{50}$) of $8.2{\mu}g/mL$ and $3.1{\mu}g/mL$, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

• Journal of Digital Convergence
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• v.11 no.10
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• pp.737-746
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• 2013

This research aim is to investigate the rate and pattern of re-using the four-digit personal identification numbers(PINs). 1313 types of PINs were observed by 224 students who took this author's classes from last 2006 to 2011 at A-university. Some students used PINs as few as 3-4 and as many as 12-13. The average is 5.86 per person. The rates of re-using PINs were calculated by each student. 87%(195/224) of students reused PINs and 64% of them reused with just only one type of PINs, 20% reused with 2 types, and about 3% reused with 3-4 types. With respect to PINs, 884 out of the total 1313 PINs were reused, that is around 64.3%. In a broad sense, a pair of slight modification of PINs were also observed, that is, new PINs were partly matched in position or size of numbers of previous PINs. And if the reuse rate falling under the slight modification of PINs, 10.4% is added, about 75% of the PINs were reused in a broad sense. The re-using rate of male students is higher than the one of female students. This paper's results may provide to make plan for hacks of passwords.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.1
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• pp.29-37
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• 2008

With the rapid development of display technology as well as the diversity of display, image quality assessment plays an important role in display color reproduction. The image quality has described by objective evaluation based on the physical measured data, such as contrast ratio, color reproduction range, color temperature, and gamma characteristics, etc., however, the final judgement is achieved by perceptual quality assessment of observers. Therefore, it is necessary to investigate the relationship between physical performance of display and perceptual image quality. Thus, in order to evaluate objectively the color reproduction characteristics of two mobile displays, TFT-LCD and AMOLED, we measured the darkroom contrast ratio of full screen, luminance and color temperature of full-screen white, full-screen gray and color linearity, tone reproduction of color scales for each RGB primary, and color reproduction range. Then the pair-wise comparison is implemented for image experts and naive observers not only to judge their preference on principal evaluation attributes but also to subjectively describe perceived image quality. Through two evaluation processes, we derived the objective bases that can prove the results of subjective image quality assessment by interconnecting physical factors that have influence on observers' preference judgements. Finally, this paper provided important information for improving the image quality and increasing preference from an observer's point of view.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.3
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• pp.60-68
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• 2008

This work describes a re-configurable 10MS/s to 100MS/s, low-power 10b two-step pipeline ADC operating at a power supply from 0.5V to 1.2V. MOS transistors with a low-threshold voltage are employed partially in the input sampling switches and differential pair of the SHA and MDAC for a proper signal swing margin at a 0.5V supply. The integrated adjustable current reference optimizes the static and dynamic performance of amplifiers at 10b accuracy with a wide range of supply voltages. A signal-isolated layout improves the capacitor mismatch of the MDAC while a switched-bias power-reduction technique reduces the power dissipation of comparators in the flash ADCs. The prototype ADC in a 0.13um CMOS process demonstrates the measured DNL and INL within 0.35LSB and 0.49LSB. The ADC with an active die area of $0.98mm^2$ shows a maximum SNDR and SFDR of 56.0dB and 69.6dB, respectively, and a power consumption of 19.2mW at a nominal condition of 0.8V and 60MS/s.

• Korean Journal of Ichthyology
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• v.29 no.2
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• pp.101-108
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• 2017

Egg development and early life history of the Korean natural monument fish Hemibarbus mylodon (Pisces: Cyprinidae) were investigated to provide basic data on biological characteristics and ecological recovery. Adult fish were collected from nature and transferred to the laboratory. For the first time, artificial maturation of females and males succeeded after 15 months of indoor culture. Mature eggs and sperm were obtained using Ovaprim injections (0.5 mL/kg) and then the eggs were fertilized using the dry method in the laboratory. The mature eggs were adhesive, turbid, and greyish; they averaged $2.21{\pm}0.06mm$ (n=30) in diameter. The embryos began to hatch about 78 h after fertilization at a water temperature $20{\pm}1^{\circ}C$, and the newly-hatched larvae were $6.6{\pm}0.75mm$ in total length (TL). At 14 days after hatching, the post-larval individuals were $13.5{\pm}0.23mm$ (TL), and their yolk sacs were completely absorbed. Twenty one days after hatching, they entered the juvenile stage and reached $13.5{\pm}0.23mm$ (TL). At 100 days after hatching, their band patterns, external form, and a pair of barbels were similar to those of adults, and they averaged $33.0{\pm}4.25mm$ (TL). The breeding technology and characteristics of early life history obtained in this study will be very helpful for conservation of H. mylodon in nature.