• Korean Journal of Agricultural Science
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• v.23 no.2
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• pp.242-249
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• 1996

In order to improve the production yield of kindorase as complex antiphlogistic agent, molecular weight of concentration membrane, membrane configration, temperature, pressure and pH of concentration solution, fouling, etc. were investigated, and the results obtained are as follows 1. High production yield was shown using 10k Dalton molecular weight cut-off concentration membrane and spiral membrane made of polysulfone. 2. And it was reasonable conditions for maintaining the concentration solution at 3Bar in pressure. 3. The optimum pH of solution for improving production yield of kindorase were the range of 7.5 to 8.5, and for obtaining better production yield, clean in place(C.I.P) of membrane was treated with 0.1N NaOH at 40 to $50^{\circ}C$ for 30minutes.

• KIPS Transactions on Computer and Communication Systems
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• v.7 no.9
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• pp.235-242
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• 2018

Various technologies have been developed to more efficiently share content such as P2P, CDN, and CCN. These technologies take a common approach that content request packets is responded by distributed network nodes or hosts, not by a single content distributor. Such approaches not only resolve network congestion around content distributors, but also make it possible to distribute content regardless of the system and network status of content distributors. However, when receiving content from distributed nodes/hosts, not from authenticated distributors, users cannot practically identify which node/host sent content to them. Due to this characteristic, various hacking caused by the malicious modification of content is possible. Therefore, to make such approaches more secure, a content authentication technique is required. In this paper, we propose a improved operation of MHT used in CCN for authenticating distributed content. Then we evaluate the proposed method by comparing its performance with the existing technology.

• Korean Journal of Microbiology
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• v.36 no.3
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• pp.242-248
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• 2000

Since the official allowance of bottled water at Korean domestic market in 1995, Pseudomo~zas aemginosu has been detected from 2.3% and 1.2% of source and products of bottled water sa~nple tested, respectively. according to the nation-wide dala froin May 1995 to December 1996. Therefore, P aeivginosa was the second most important parameter, next to colifoi~ns, anlong the bacieriological parameters regulated for bonled water. The official standard method initially adopted the Japanese officlal method and Standard Methods of the US, w~hich is using aspai-agiii-acetamid mnedia(A-A method). how eve^; the method showed low specificity regardless of the high sensitivity. The $42^{\circ}C$ growth test was the best biochemical featu1-e differentiating the P uelarginosu 6-om P aei-uginosa-like species such as P puririir and P Jhoi.escens amo1zg the other characierisiics such as fluorescence pigment_ pyocyanin, casein hy&olysis, etc. Thel-efore, addition of the $42^{\circ}C$ growth Lest in advance ofthe biochemical identification test, when sainple is positive by A-A method, should strengthen the spec~IiclQ w~tli ~ninin~um addition of testing load.

• Journal of Korean Academic Society of Home Health Care Nursing
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• v.28 no.3
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• pp.233-242
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• 2021

Purpose: This study investigated the degree of, and factors influencing the spiritual care among home healthcare nurses. Methods: The subjects were 168 advanced practice nurses working in 94 hospital-based facilities for home health care located in Seoul, Gyeonggi-do, and Incheon Metropolitan City. Data were collected from January 14, 2019, to March 7, 2019, using structured questionnaires on spiritual care and related variables based on the literature. Data were analyzed by t-test, one-way ANOVA, Pearson correlation, and multiple regression analysis (stepwise) using SPSS Version 23.0 program. Results: The results showed that the degree of spiritual care of home health care nurses was 3.44 points out of a possible 5 points. Factors affecting nurses' spiritual care were spiritual well-being(𝛽=.36, p<.001), recognition of spiritual care as the nature of nursing(𝛽=.17, p=.016) and role of nurses(𝛽=.22 p=.002), regular-based education for spiritual care(𝛽=.23, p<.001), patients' discomfort with spiritual discussion(𝛽=-.18, p=.001), and nurses' experience in home health care area(𝛽=.14, p=.010), which explained 57.2% of the observed variation in spiritual care. Conclusion: This study suggests the need to develop regular-based education programs for facilitating spiritual care and strengthening home health care nurses' spiritual well-being and their positive perceptions toward spiritual care.

• Korean Journal of Microbiology
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• v.34 no.4
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• pp.236-242
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• 1998

While bacteriophage P2-P4 system is very useful experimental tool for the study of viral capsid assembly, there is no useful plasmid vector for the DNA manipulation in bacteriophage P2-P4 system. In this study, a new vector plasmid, P4 ash8 (sid71) kmr, was constructed by swapping the non-essential region of P4 DNA for kanamycin resistance(kmr) gene cassette of plasmid pUC4-K. P4 ash8 sid71 was starting material for the construction, since it tends to be maintained as a plasmid in the absence of the helper phage. The total size of this chimera was designed to be packaged into P4 or P2 size heads with induction by P2 infection. The conversion of plasmid P4 ash8 (sid71) kmr to bacteriophage was proved by burst size determination experiment and CsCl buoyant equilibrium density gradient experiment. Integrase destructed P4 derivative, P4 ash8 sid71 kmr intS, was able to be constructed easily by in vitro DNA manipulation of P4 ash8 sid71 kmr. The plasmid stability experiment with P4 ash8 sid71 kmr if/tS showed that the integrase of P4 affects the stable maintenance of plasmid P4 state.

• Korean Journal of Poultry Science
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• v.37 no.1
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• pp.1-8
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• 2010

An experiment was conducted to investigate the effect of dietary supplementation of rare earth, individually and in combination with colloidal silver on growth performance, nutrient digestibility, carcass characteristics and immune response in broiler chicks. A total of 3,872 day-old chicks were randomly allotted to four dietary treatments in a randomized complete block design. There were four pens per treatment and 242 chicks per pen. The dietary treatments were T1) CON (control diet), T2) colloidal silver (control diet+20 ppb colloidal silver), T3) rare earth elements (control diet+500 ppm rare earth), T4) colloidal silver +rare earth elements (control diet+20 ppb colloidal silver+500 ppm rare earth). There were no significant differences in feed intake and feed conversion ratio (FCR) among dietary treatments during the whole experimental period (0 to 5 weeks). Body weight gain was greatest in birds fed T3 and T4 diets (p<0.01). Apparent digestibility of dry matter, crude protein, ether extract and calcium were greatest in birds fed T4 diet (p<0.05), while apparent digestibility of phosphorus was lowest in birds fed T3 diet (p<0.05). There was no significant difference in bone strength, carcass characteristics and immune response among dietary treatments. Dry matter content of bone was greatest in birds fed T4 diet (p<0.05), and phosphorus content of bone was greater in birds fed T3 diet than in birds fed T2 and T4 diets (p<0.05). Thus, the results of this study suggest that rare earth elements supplementation, individually and in combination with colloidal silver could improve performance of broilers.

• Journal of Life Science
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• v.21 no.2
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• pp.242-250
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• 2011

Although insulin-like growth factor-I (IGF-I) and androgen receptor (AR) coactivators are well known effectors of skeletal muscle, the molecular mechanism by which signaling pathways integrating AR coactivators and IGF-I in skeletal muscle cells has not been previously examined. In this study, the effects of IGF-I treatment on the gene expression of AR coactivators in the absence of AR ligands and the roles of the p38 MAPK and ERK1/2 signaling pathways in IGF-I-induced AR coactivators induction were examined. C2C12 cells were treated with 250 ng/ml of IGF-I in the presence or absence of specific inhibitors p38 MAPK (SB203580) or ERK1/2 (PD98059). Treatment of C2C12 cells with IGF-I resulted in increased in GRIP-1, SRC-1, and ARA70 protein expression. The levels of GRIP-1, SRC-1, and ARA70 mRNA were also significantly increased after 5min of IGF-I treatment. IGF-I-induced AR coactivator proteins were significantly blocked by pharmacological inhibitors of p38 MAPK and ERK1/2 pathways. However, there was no significant effect of those inhibitors on IGF-I-induced mRNA level of AR coactivators, suggesting that AR coactivators are post-transcriptionally regulated by IGF-I. Furthermore, the present results suggest that IGF-I stimulates the expression of AR coactivators by cooperative activation of the p38 MAPK and ERK1/2 pathways in C2C12 mouse skeletal muscle cells.

• Transactions on Electrical and Electronic Materials
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• v.9 no.6
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• pp.237-242
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• 2008

The effects of the process parameters in the molding of aspheric glass lenses for camera phone modules have been investigated experimentally. The molding conditions were optimized with respect to the form accuracy (PV) (the response variable) of the molded lens. The experimental conditions were obtained by employing a factorial design method. From the analysis of variance (ANOVA) and P-value (significance level), the slow cooling rate was found to affect the response variable most significantly. The lens molded under the optimum molding condition showed a transcription ratio of 93.4%.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1046-1048
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• 2003

A polymerase chain reaction (PCR) assay was developed to differentiate buffalo meat from the meat of Ceylon spotted deer (Axis axis ceylonensis), Ceylon sambhur (Cervus unicolor unicolor), cattle (Bovine), goat (Caprine), pig (Porcine), and sheep (Ovine). A set of primers were designed according to the sequence of the mitochondrial cytochrome b gene of bubalus bubalis and by PCR amplification a band of approximately 242 bp band was observed with buffalo DNA. These primers did not cross-react with DNA of other animal species tested in the study under the specified reaction conditions. A band of 649 bp was observed for all animal species tested when DNA was amplified with the universal primers indicating the presence of mitochondrial DNA in the samples. The technique was sensitive enough to identify rotten (10 days post slaughter), dried and cooked buffalo meat. The absence of a cross reaction with human DNA using the buffalo specific primers eliminates possible false positive reactions.

• Journal of Technologic Dentistry
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• v.37 no.4
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• pp.235-242
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• 2015

Purpose: The aims of this study are compare with microstructure and mechanical properties of Co-Cr-Mo alloys fabricated by powder metallurgy(P/M) process and casting process respectively. Methods: Microstructure and micro-hardness were tested by SEM and Vickers Hardness Tester. The sintered specimen was produced by furnace-coolling after sintering, however the casting specimen were produced thru air-cooling and water-cooling after the casting. For observation of phase transformation during sintering, DSC analyzing was carried out. Results: Mean pore size of sintered Co-Cr-Mo alloy was $4.32{\mu}m$ and that of casting alloy was $1.63{\mu}m$. Hardness of sintered alloy was lower than water-quenched casting alloy. Conclusion: Proper sintering temperature of Co-Cr-Mo alloy was above $1,200^{\circ}C$ and pore size of casting specimen were finer than sintered specimen, but hardness were similar.