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Effect of Triticale Dried Distillers Grains with Solubles on Ruminal Bacterial Populations as Revealed by Real Time Polymerase Chain Reaction

  • Wu, R.B.;Munns, K.;Li, J.Q.;John, S.J.;Wierenga, K.;Sharma, R.;Mcallister, T.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.11
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    • pp.1552-1559
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    • 2011
  • Real time PCR was used in this study to determine the effect of triticale dried distillers grains with solubles (TDDGS) as a replacement for grain or barley silage in finishing diets on the presence of six classical ruminal bacterial species (Succinivibrio dextrinosolvens, Selenomonas ruminantium, Streptococcus bovis, Megasphaera elsdenii, Prevotella ruminicola and Fibrobacter succinogenes) within the rumen contents of feedlot cattle. This study was divided into a step-wise adaptation experiment (112 days) that examined the effects of adaptation to diets containing increasing levels of TDDGS up to 30% (n = 4), a short-term experiment comparing animals (n = 16) fed control, 20%, 25% or 30% TDDGS diets over 28 days, and a rapid transition experiment (56 days) where animals (n = 4) were rapidly switched from a diet containing 30% TDDGS to a barley-based diet with no TDDGS. It was found that feeding TDDGS as replacement for barley grain (control vs. 20% TDDGS) decreased 16S rRNA copy numbers of starch-fermenting S. ruminantium and S. bovis (p<0.001 and p = 0.04, respectively), but did not alter 16S rRNA copy numbers of the other rumen bacteria. Furthermore, feeding TDDGS as a replacement barley silage (20% vs. 25% and 30% TDDGS) increased 16S rRNA copy numbers of S. ruminantium, M. elsdenii and F. succinogenes (p<0.001; p = 0.03 and p<0.001, respectively), but decreased (p<0.001) the 16S rRNA copy number of P. ruminicola. Upon removal of 30% TDDGS and return to the control diet, 16S rRNA copy numbers of S. ruminantium, M. elsdenii and F. succinogenes decreased (p = 0.01; p = 0.03 and p = 0.01, respectively), but S. dextrinosolvens and S. bovis increased (p = 0.04 and p = 0.009, respectively). The results suggest that replacement of TDDGS for grain reduces 16S rRNA copy numbers of starch-fermenting bacteria, whereas substitution for barley silage increases 16S rRNA copy numbers of bacteria involved in fibre digestion and the metabolism of lactic acid. This outcome supports the contention that the fibre in TDDGS is highly fermentable.

Relationship between Delegation Preparedness and Job Satisfaction of Nurses in Comprehensive Nursing Care Service Units (간호·간병통합서비스병동 간호사의 위임준비성과 직무만족 간의 관계)

  • Kim, Sunghui;Kim, Miyoung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.9
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    • pp.103-112
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    • 2020
  • This study examined the relationship between delegation preparedness and job satisfaction in comprehensive nursing care service wards. Data were collected from 126 nurses who had been working in the comprehensive nursing care wards of five general hospitals located in Seoul and Gyeonggi Province, from May 26 to June 7, 2017. Data were analyzed using independent t-test, one-way ANOVA, and Pearson's correlation coefficients. The delegation education showed a significant difference in delegation preparedness (t=-2.77, p=.006). A statistically significant positive correlation was observed between delegation preparedness and job satisfaction (r=.43, p<.001). Job satisfaction was significantly correlated with understanding the task (r=.26, p=.003), delegation of the task classification (r=.45, p<.001), understanding the delegation (r=.35, p<.001), and delegation skill (r=.34, p<.001), which are the sub-domains of delegation preparedness. Delegation preparedness was correlated significantly with interactions (r=.46, p<.001), task requirement (r=.36, p<.001), professional status (r=.33, p<.001), administration (r=.31, p<.001), and pay (r=.20, p=.026), which are sub-domains of job satisfaction. These results highlight the need for schools and clinical settings to provide continuous education to improve the delegation preparedness for nurses in comprehensive nursing care wards.

Personality Types of Nurses and the Relation between Self-Efficacy and Clinical Performance Ability (간호사의 성격유형, 자기효능감과 임상수행능력 간의 관계)

  • Park, Young-Mi;Ju, Hyeon-Jeong
    • Journal of Digital Convergence
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    • v.14 no.11
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    • pp.333-345
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    • 2016
  • This study was to determine the relationship between nurse DISC personality types, self-efficacy, clinical performance. Data were collected from 302 nurses in 5 hospitals in K city. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient. With regard to self-efficacy and clinical performance, D type showed a higher score than type I type and S type. The lower region of the clinical performance showed the nursing process, nursing skills, higher score than the D Type S Type in professional development. With regard to personality and self-efficacy, and clinical performance, the type D (r=.56, p<.01), I type (r=.66, p<.01), S type (r=.6, p<.01) showed a positive correlation with type C (r=.52, p<.01). As shown by these results, each personality type has a different correlation with self-efficacy and clinical performance ability. This factor should be considered in the assignment of nurses to departments or individual training programs so that they can deliver the most effective job performance.

T.P.H.A Positive Rate of General and Ordinary Health Examine (일반(一般) 정기(定期) 건강진단자(健康診斷者)의 T.P.H.A양성율(陽性率))

  • Lee, Hoa-Suk;Lee, Soo-Young;Kim, Kang-Seuck
    • The Journal of the Korean life insurance medical association
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    • v.2 no.1
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    • pp.253-259
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    • 1985
  • In medical selection of Daehan Education Insurance, for 6 months from June 1st, 1984 to November 30th, 1984 we examined 2523 persons, who were supposed to receive general test and Normal subject test Then we did syphilis test by selecting the way of R.P.R. card test and repeatedly confirming the examination serologically. The results are as follows: 1) As results of R.P.R. card test, 30 out of 1592 males are positive(1.89%) and 10 out of 931 females are positive(1.1%). 40 out of total 2523 persons are positive(1.6%). 2) As results of T.P.H.A, 28 out of 1592 males are positive(1.76%) and 9 out of 931 females are positive. 37 out of total 2523 persons are positive(1.47%). 3) When R.P.R. card test is compared with T.P.H.A test, 40 persons by R.P.R. showed that 2 males and female are negative and only 37 persons are positive in T.P.H.A test. And so the result agreement rate of R.P.R. card test and T.P.H.A test showed 92.5%. 4) In the age-distribution males have high positive rates in 30's and 40's and females have high positive rates in 20's and 40's when the subjects are normal.

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ON MARCINKIEWICZ'S TYPE LAW FOR FUZZY RANDOM SETS

  • Kwon, Joong-Sung;Shim, Hong-Tae
    • Journal of applied mathematics & informatics
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    • v.32 no.1_2
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    • pp.55-60
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    • 2014
  • In this paper, we will obtain Marcinkiewicz's type limit laws for fuzzy random sets as follows : Let {$X_n{\mid}n{\geq}1$} be a sequence of independent identically distributed fuzzy random sets and $E{\parallel}X_i{\parallel}^r_{{\rho_p}}$ < ${\infty}$ with $1{\leq}r{\leq}2$. Then the following are equivalent: $S_n/n^{\frac{1}{r}}{\rightarrow}{\tilde{0}}$ a.s. in the metric ${\rho}_p$ if and only if $S_n/n^{\frac{1}{r}}{\rightarrow}{\tilde{0}}$ in probability in the metric ${\rho}_p$ if and only if $S_n/n^{\frac{1}{r}}{\rightarrow}{\tilde{0}}$ in $L_1$ if and only if $S_n/n^{\frac{1}{r}}{\rightarrow}{\tilde{0}}$ in $L_r$ where $S_n={\Sigma}^n_{i=1}\;X_i$.

Quality of Life and Related Factors in Caregivers of Attention Deficit Hyperactivity Disorder Patients (주의력결핍 과잉행동장애 환아 보호자의 삶의 질과 관련요인)

  • Jeong, Jong-Hyun;Hong, Seung-Chul;Han, Jin-Hee;Lee, Sung-Pil
    • Korean Journal of Psychosomatic Medicine
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    • v.13 no.2
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    • pp.102-111
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    • 2005
  • Objective : The purpose of this study was to investigate the quality of life and it's related factors in caregivers of attention deficit hyperactivity disorder patients. Methods : The subjects were 38 attention deficit hyperactivity disorder patients' caregivers(mean age : $37.5{\pm}6.5$, 38 women). Patients were diagnosed with DSM-IV-TR ADHD criteria. Korean version of WHOQOL-BREF(World Health Organization Quality of Life assessment instrument Abbreviated Version) was used for assessment. Results : 1) No significant differences were found in the score of WHOQOL-BREF, overall QOL, physical health domain, psychological domain, social relationships domain and environmental domain between caregiver and control group. 2) The score of Activity of daily living facet$(3.0{\pm}0.7\;vs.\;3.6{\pm}0.7)(p=0.008)$ and self-esteem facet $(2.8{\pm}0.7\;vs.\;3.3{\pm}0.7)(p=0.049)$ were significantly decreased in caregivers of ADHD. 3) Total score of WHOQOL-BREF(r=0.437, p=0.007) and physical health domain(r=0.370, p=0.024) were correlated with caregiver's educational age. 4) In the psychological domain, the score of self-esteem facet(r=-0.337, p=0.039) and thinking, learning, memory & concentration facet(r=-.341, p=0.036) were decreased with caregiver's age. 5) The score of environmental domain were significantly increased with caregiver's educational age (r=0.482, p=0.003), but decreased with patient's age(r=0.328, p=0.044). Conclusion : Although the quality of life in caregivers of ADHD patient had not significantly decreased than control, the quality of lift were positively correlated with educational age of caregives, and negatively correlated with chronological age of caregivers and children. Above results suggest that physicians should consider integrated approaches for caregiver's subjective quality of life in the management of ADHD.

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Soluble IL-2R, IFN-$\gamma$ and Neopterin as Immunologic Markers in Patients with Tuberculosis (결핵 환자에서 면역학적 지표로서의 sIL-2R, IFN-$\gamma$, Neopterin에 관한 연구)

  • Ryu, Yon-Ju;Ryu, Kum-Hei;Kim, Su-Hyun;Lee, Jong-Soo;Cheon, Seon-Hee;Seoh, Ju-Young
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.3
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    • pp.294-308
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    • 2002
  • Background : The cell-mediated immune response plays an important role in tuberculosis. After being activated by mycobacterial antigens, T lymphocytes express a high affinity receptor (IL-2R) for interleukin-2 (IL-2) on their own surface and release a soluble fraction of the IL-2 receptor (sIL-2R) from the cell membrane into the circulation. Neopterin is a metabolite of guanosine-triphosphate, which is produced by stimulated macrophages under the influence of IFN-$\gamma$ with a T lymphocyte origin. Therefore, the utility of sIL-2R, IFN-$\gamma$ and the neopterin levels as immunologic indices of the cell-mediated immune response and severity of disease in patients with pulmonary tuberculosis was assessed. Methods : The serum sIL-2R, IFN-$\gamma$ and neopterin levels were measured in 39 patients with pulmonary tuberculosis, 6 patients with tuberculous lymphadenitis prior to treatment and 10 healthy subjects. The serum and pleural sIL-2R, neopterin and ADA levels were measured in 22 patients with tuberculous pleurisy. The patients with pulmonary tuberculosis were divided into a mild, moderate and severe group according to the severity by ATS guidelines. To compare the results from these patients with those of the pretreatment levels, the sIL-2R, IFN-$\gamma$ and neopterin levels were measured in 36 of the 39 patients(1 patient, expired; 2 patients were referred to a sanitarium) with pulmonary tuberculosis after 2 months of treatment. Results : 1) the serum sIL-2R and IFN-$\gamma$ levels were elevated in patients with tuberculosis when compared to those of healthy subjects (p>0.05). The neopterin concentration in the serum was significantly lower in patients with pulmonary tuberculosis($2967{\pm}2132.8$ pg/ml) than in healthy controls($4949{\pm}1242.1$ pg/ml)(p<0.05). 2) In the pulmonary tuberculosis group, the serum sIL-2R and IFN-$\gamma$ levels were higher in patients with severe disease than those in patients with mild and moderate disease. However, the neopterin levels declined as the pulmonary tuberculosis became more severe (p<0.01). 3) The mean serum sIL-2R and IFN-$\gamma$ levels declined from $1071{\pm}1139.4$ U/ml to $1023{\pm}1920.9$ U/ml(p>0.05), $41{\pm}52.8$ pg/ml to $22{\pm}23.9$ gm/ml(p<0.05), respectively, after 2 month of treatment. The mean serum neopterin levels increased from $3158{\pm}2272.6$ pg/ml to $3737{\pm}2307.5$ pg/ml(p>0.05) after a 2 month of treatment. These findings were remarkable in the severe group of pulmonary tuberculosis with a clinical correlation. 4) In the patients with tuberculous pleurisy, the serum sIL-2R and ADA were significantly higher than those in the pleural fluid, However, the neopterin levels in the sera and pleural effusion were similar. Conclusion : On the basis of this study, sIL-2R, IFN-$\gamma$ and neopterin measurements may not only provide an insight into the present state of the cell-mediated immune response, but also serve as parameters monitoring of the prognosis of the disease, particularly in patients with severe pulmonary tuberculosis. In addition, an assay of the pleural sIL-2R levels might signal a stimulated local immunity including T cell activation in the tuberculous pleural effusion.

ON DISCONTINUOUS ELLIPTIC PROBLEMS INVOLVING THE FRACTIONAL p-LAPLACIAN IN ℝN

  • Kim, In Hyoun;Kim, Yun-Ho;Park, Kisoeb
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.6
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    • pp.1869-1889
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    • 2018
  • We are concerned with the following fractional p-Laplacian inclusion: $$(-{\Delta})^s_pu+V(x){\mid}u{\mid}^{p-2}u{\in}{\lambda}[{\underline{f}}(x,u(x)),\;{\bar{f}}(s,u(x))]$$ in ${\mathbb{R}}^N$, where $(-{\Delta})^s_p$ is the fractional p-Laplacian operator, 0 < s < 1 < p < $+{\infty}$, sp < N, and $f:{\mathbb{R}}^N{\times}{\mathbb{R}}{\rightarrow}{\mathbb{R}}$ is measurable with respect to each variable separately. We show that our problem with the discontinuous nonlinearity f admits at least one or two nontrivial weak solutions. In order to do this, the main tool is the Berkovits-Tienari degree theory for weakly upper semicontinuous set-valued operators. In addition, our main assertions continue to hold when $(-{\Delta})^s_pu$ is replaced by any non-local integro-differential operator.

Menopausal Symptoms and Depression Among Midlife Women (중년여성의 폐경 증상과 우울)

  • Lee, Hung-Sa;Kim, Chun-Mi
    • Research in Community and Public Health Nursing
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    • v.21 no.4
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    • pp.429-438
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    • 2010
  • Purpose: This study was descriptive survey research to examine the factors affecting menopause, depression and menopausal symptoms, and the relations among these factors in South Korean midlife women. Methods: The subjects were 319 midlife women in Daegu City. Measures were Zung's Self-Report Depression Scale (1979) to check depression, and the Midlife Women's Symptom Index developed by Im (2006) to check menopausal symptoms. Data were collected from June 1 to July 31, 2010. Collected data were analyzed by mean, standard deviation, and Pearson's correlation coefficient through SPSS/WIN 18.0. Results: There was a statistically significant correlation between depression and menopausal symptoms (r=.692, p<.001). The factors affecting depression were age (r=.194, p<.001), income (r=-.307, p<.001), exercise (r=-.602, p<.001), and menopausal status (r=.224, p<.001). The factors affecting menopausal symptoms were exercise (r=-.158, p<.037), menstrual pain (r=-.171, p = .004) and menopausal status (r=.222, p<.001). Conclusion: Intervention programs for decreasing menopausal symptoms in midlife women should be focused on relieving menopausal pain and encouraging excise from youth. Further studies will be needed to examine the relations among other factors including living style, area and menopausal symptoms.

RESOLVENT INEQUALITY OF LAPLACIAN IN BESOV SPACES

  • Han, Hyuk;Pak, Hee Chul
    • Journal of the Chungcheong Mathematical Society
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    • v.22 no.1
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    • pp.117-121
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    • 2009
  • For $1{\leq}p$, $q{\leq}{\infty}$ and $s{\in}\mathbb{R}$, it is proved that there exists a constant C > 0 such that for any $f{\in}B^{s+2}_{p,q}(\mathbb{R}^n)$ $${\parallel}f{\parallel}_{B^{s+2}_{p,q}(\mathbb{R}^n)}{\leq}C{\parallel}f\;-\;{\Delta}f{\parallel}_{B^{s}_{p,q}(\mathbb{R}^n)}$$, which tells us that the operator $I-\Delta$ is $B^{s+2}_{p,q}$-coercive on the Besov space $B^s_{p,q}$.

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