• Applied Biological Chemistry
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• v.24 no.4
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• pp.230-237
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• 1981

For the investigation of efficient use of fertilizer nitrogen NPK simple trials were carried out using newly bred Tongil line(japonica indica hybrid selection) and the leading local varieties with sulfur coated area in fields for two years. The yield of Tongil line was higher than that of leading local varieties by 16% in the cool year and 23% is warm year with high solar radiation from NPK simple trials including SCU and 2-split application which were carried in 130 fields at 32 locations. Warm year increased yield by 9% in Tongil but only 3% in the local than the cool year did. Optimum nitrogen level was higher in Tongil by 3.8㎏ in the cool year and 5.5㎏ in warm year than for the local. Yearly variation of optimum nitrogen in the carne variety was greater than varietal variation in the same Year. Two-split application resulted in 1 to 2% of inconsistent yield variation. Sulfur coated urea increased yield by average 2 to 4% even at 20% reduced rate of nitrogen. It was remarkably effective in same $(27{\sim}39%)$, virgin (20%) and unmatured (10%) soils.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.3
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• pp.211-219
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• 2005

Vegetation composition and structure of Sorbus commixta - native forests of South Korea were studied using phytosociological procedures and its ecological characteristics analyzed with special respect to species correlations, importance values, and constancy classes. Vegetation types were divided (great division) into Tripterygium regelii - Quercus mongolica (Inland high mountain type) and Acer takesimense - Fague crenata var. multinervis (Ulleung Island type) and ten accompanying vegetation units. In between S. commixta and life-farms, 120 correlations were positive with 23 of these having a p-value < 0.01 for trees, 21 for shrubs, 10 for woody vines, and 25 for herbs. In trees, there was a positive correlation between S. commixta and A. takesimense and T. insularis on the $1\%$ level. In shrubs, there was a positive correlation between S. commixta and Sasa kurilensis, Callicarpa japonica, Ligustrum foliosum on the $5\%$ level. In woody vines, there was a negative correlation between S. commixta and Tripterygium regelii and Actinidia rufa on the $1\%$ level. In herbs, there was a positive correlation between S. commixta and Majanthemim dilatatum and Solidago virga-aurea var. gigantea on the $1\%$ level.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.6
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• pp.123-132
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• 2016

The study was to introduce the revegetation measures for the improvement of the landscape in October, 2012 on the slope; two and four years after the introduction, the slope was examined to see the status of the revegetation. The result is as follows. The result from examining the soil on the slope, the soil was Loamy sand; which infers that the soil is in a poor condition for improving the scenery. Examining the characteristics of the vegetation-base materials, the soil acidity was pH 6.4 to 6.7; and the soil hardness was 21 to 24mm (hardness quotient of soil), this can be considered a favourable condition, which has no effect on the growth and development of plants. When it comes to the discovery of the species in the area, Lotus corniculatus var., Silene armeria L., Papaver rhoeas, Trifolium pratense L. developed and grew in spring after two years. And in the summer, Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense were founded. And there were Lotus corniculatus var., Dianthus chinensis L., Aster yomena, Coreopsis tinctoria, Trifolium pratense L. in the autumn. According to the examination conducting after four years, Lotus corniculatus var., Dianthus chinensis L., Silene armeria, Cosmos bipinnatus, Coreopsis drummondii L., Papaver rhoeas, Trifolium pratense L. were founded in the spring, and Lotus corniculatus var., Dianthus chinensis L., Cosmos bipinnatus, Coreopsis drummondii L., Trifolium pratense grew in the summer. Lotus corniculatus var., Dianthus chinensis L., Astragalus sinicus, Aster yomena, Coreopsis tinctoria, Trifolium pratense L. were discovered in the autumn. Among the exotic species founded in the area, were Festuca arundinacea, Lolium perenne, Festuca rubra. When it comes to the protection of vegetation and dominance, Lotus corniculatus var., Metaplexis japonica, Coreopsis drummondii L. are the dominant species among the spring plants; and Lotus corniculatus var., Trifolium repens L., Erigeron annuus (L.) Pers. were the dominant plants in the summer. In the autumn, Lotus corniculatus var., Coreopsis drummondii L., Poa pratensis appear to be the dominant plants. The colonies of Pinus densiflora and Castanea crenata var. were formed around the South Sejong IC. Part of the area is occupied by the colony of Alnus hirsuta Turcz. ex Rupr. In terms of shrub, there were Indigofera pseudo-tinctoria, Robinia pseudoacacia, and Rosa polyantha var. genuina NAKA. As ground cover plants, Sasa borealis, Rubus crataegifolius Bunge were founded.

• Korean Journal of Poultry Science
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• v.35 no.1
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• pp.29-37
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• 2008

This study was evaluated the oxidative stability of the breast and leg meat from chicken fed extract of medicinal herb mix (Lonicera japonica Thunberg : Morus alba L. : Coptis chinensis = 48.5 : 48.5 : 3.0%) and antibiotics (Albac G150). Ross chickens were divided into three treatments: basal diet (control), basal diet with antibiotics (0.05%) and basal diet with extract of medicinal herb mix (0.3%). They were fed the experimental diets for 35 days and slaughtered. After that, the breast and leg meat samples were stored at $4^{\circ}C$ for 10 days. Total phenol content of breast meat in Ross chicken fed extract of medicinal herb mix was higher than that of antibiotics treatment and control during storage. DPPH radical scavenging activity was the highest in leg meat of Ross chicken fed extract of medicinal herb mix at 0 day. $ABTS^+$ reduction activity was higher in chicken meat fed extract of medicinal herb mix than antibiotics treatment and control. This results showed that the diet with extract of mixed medicinal herb was more effective in oxidative stability of chicken meat than basal diet and diet with antibiotics.

• Journal of Plant Biotechnology
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• v.45 no.1
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• pp.63-70
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• 2018

Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.

• Asian Journal of Atmospheric Environment
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• v.6 no.4
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• pp.268-274
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• 2012

To perform quick measurements of black carbon (BC) particles deposited on foliar surfaces of forest tree species, we investigated an optical method for measuring the amount of BC extracted from foliar surfaces and collected on quartz fiber filters. The seedlings of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica were exposed to submicron BC particles for one growing season (1 June to 7 December 2009). At the end of the growing season, the leaves or needles of the seedlings were harvested and washed with deionized water followed by washing with chloroform to extract the BC particles deposited on the foliar surfaces. The extracted BC particles were collected on a quartz fiber filter. The absorption spectrum of the filters was measured by spectrophotometer with an integrating sphere. To obtain the relationship between the absorbance of the filter and the amount of BC particles on the filter, the amount of BC particles on the filter was determined as that of elemental carbon (EC) measured by a thermal optical method. At wavelengths below 450 nm, the absorption spectrum of the filter showed absorption by biological substances, such as epicuticular wax, resulting in the low coefficient of determination ($R^2$) in the relationship between the amount of EC on the filter ($M_{EC}$, ${\mu}g\;C\;cm^{-2}$ filter area) and the absorbance of the filter. The intercept of the regression line between $M_{EC}$ and the absorbance of the filter at 580 nm ($A_{580}$) was closest to 0. There was a significant linear relationship between the $A_{580}$ and $M_{EC}$ ($R^2$=0.917, p<0.001), suggesting that the amount of BC particles collected on the filter can be predicted from the absorbance. This optical method might serve as a simple, fast and cost-effective technique for measuring the amount of BC on foliar surfaces.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.2
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• pp.287-292
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• 2014

The objectives of this study were to examine the effects of red clay-processed nano-materials (RCPM) on the quality characteristics of eel (Anguilla japonica). Both eels treated with RCPM and control were farm-raised under identical environments and were commercially processed. General components, texture, nutrients, antioxidant activities, and sensory analysis were performed. RCPM-treated eels showed significantly higher DPPH radical scavenging activities and FRAP values than the of control, indicating higher antioxidant activities of eels raised by RCPM. The amounts of niacin and tocopherol in RCPM-treated eels were higher than those of the control. For analysis of freeze-thaw stability, RCPM-treated eels showed more stable texture over freeze-thaw three treatment cycles. Descriptive panelists perceived eels raised by RCPM to be less oily than the control. Overall, RCPM exhibited positive effects on the quality of farm-raised eels. Therefore, RCPM would be of benefit to produce high value-added eels of premium quality.

• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.166-177
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• 1988

To investigate the structure of the plant community of the Naejang Temple District in Mt. Naejang National Park, 22 plots were set up by the vegetation physiognomy and vegetation analysis by four kinds of ordination techniques(PO, PCA, RA and DCA) was carried out. Pure and mixed forest community of Quercus variabilis and Q. mongolica were major forest communities and each of them covered 31.27 and 20.77%, respectively. The degree of human disturbance of vegetation 9, 8, 7 and 6 area covered 30.9, 67.0, 0 and 0.02%, respectively. According to stand dynamic analysis by DBH class distribution, the present Pinus densiflora and Quercus communities may be succeeded to Carpinus laxiflora communities. DCA was the most effective method of this study. DCA ordination showed that successional trends of tree species seem to be from P. densiflora through Q. variabilis, Q. aliena, Sorbus alnifolia, Platycarya strobilacea to Carpinus laxiflora, Fraxinus rhynchophylla, Zelkova serrata, Cornus controversa in the tree layer, and from Rhododendron mucronulatum, Rh. schlippenbachii, Lespedeza cyrtobotrya, Rhus trichocarpa through Styrax japonica, Daphniphyllum macropodum, Sapium japonicum to Euonymus oxyphyllum, E. sieboldiana, Lindera erythrocarpa in the subtree layer.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.419-428
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• 2013

We identified the diversity, geographical distribution, ecological characteristics of birds, trees, and flowers species designated as local government symbols as a means for understanding public awareness on biodiversity. A total of 114 species including 48 birds, 40 flowers, 36 tree species belonging to 40 orders 59 families 90 genus were designated as natural symbols. Pica pica (L.) in birds, Rhododendron schlippenbachii Maxim. among flowers, have also been designated as symbols. The tree Ginkgo biloba L. was designated with the highest frequency. Local governments located coastal area were selected bird species of the Laridae family and the tree species Camellia japonica L., of Pinaceae as their natural symbols. In contrast, local governments located inland have designated resident bird species such as P. pica and Columba rupestris (Pallas) and flower species from the Ericaceae as natural symbols widely distributed and easily observed throughout South Korea. However, many local governments have designated the same species as a natural symbol based on their size color and public appeal and popularity. The information about the popular species and their ecological traits are useful for establishing the criteria as flagship or iconic species selection and their roles in habitat conservation.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.447-466
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• 2019

The orchard flora where perennial fruit trees are grown may be different than in arable fields where annual crops are grown. The study focused on the floristic composition and characteristics of orchards in South Korea. The flora surveys were conducted in 36 areas in nine provinces at two times (May-June and August-September) in 2014. The results showed that the vascular orchard plants in South Korea included 466 taxa, which contained 91 families, 278 genera, 420 species, two subspecies, 39 varieties, four forms, and one hybrid. Among the 91 families, Compositae was the most diverse in species (66 taxa), followed by Gramineae (51 taxa), Leguminosae (28 taxa), Cyperaceae (18 taxa), Polygonaceae (17 taxa), Cruciferae (16 taxa), and Labiatae (14 taxa). Based on the occurrence frequency of each species, Digitaria ciliaris (Retz.) Koel. (100%) was the highest, followed by Acalypha australis L. (94.4%), Commelina communis L. (94.4%), Persicaria longiseta(Bruijn) Kitag.(91.7), Capsella bursa-pastoris(L.) L. W. Medicus(91.7%), Erigeron annuus (L.) Pers. (91.7%), Mazus pumilus (Burm. f.) Steenis (86.1%), Artemisia princeps Pamp. (86.1%), Cyperus microiria Steud. (86.1%), Stellaria aquatica (L.) Scop. (83.3%), Stellaria media(L.) Vill.(83.3%), and Echinochloa crus-galli (L.) P. Beauv.(83.3%). The biological type of orchards in South Korea was determined to be Th-₅-D₄-e type. Rare plants were found six taxa: Cinnamomum camphora (L.) J. Presl, Aristolochia contorta Bunge, Melothria japonica (Thunb.) Maxim., Ardisia crenata Sims, Gnaphalium hypoleucum DC., and Aster koraiensis Nakai. Eighty-five taxa contained naturalized plants composed of 23 families, 58 genera, 80 species, four varieties, and one form. The urbanization and naturalization indices were 26.3% and 18.2%, respectively.