• 한국식물병리학회지
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• 제14권2호
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• pp.177-183
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• 1998

A DNA fragment which is involved in tolassin production was cloned to obtain a molecular marker of Pseudomonas tolaasii, a casual agent of bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). Tolaasin is a lipodepsipeptide toxin and known as a primary disease determinant of the P. tolaasii. It is responsible for formation of white line in agar when P. tolaasii were cultured against white line reacting organisms (WLROs). White line negative mutants (WL-) were generated by conjugation between rifampicin resistant strain of P. tolaasii and E. coli carrying suicidal plasmid pSUP2021 : : Tn5. The ability of tolaasin production of the WL- mutants was examined by hemolysis test, pathogenicity test, and high pressure liquid chromatography (HPLC) analysis of culture filtrate. All of the WL- mutants were lost the ability of tolaasin production (Tol-). Genomic library of the Tol- mutant was constructed in pLAFR3 and the cosmid clone containing Tn5 was selected. DNA fragment fro franking region of Tn5 was cloned from the plasmid and used as a probe in Southern blot. DNA-DNA hybridization with the probe to total DNA from group of bacteria ecologically similar to P. tolaasii including WLORs, fluorescent Pseudomonads isolated from oyster mushroom, P. agarici, P. gingeri, and some of other species of Psedomonas showed that some of the tested bacteria do not have any hybridized band and others have bands sowing RFLP. The cloned DNA fragment or its nucleotide sequence will be useful in detection and identification of the P. tolaasii.

• 농업과학연구
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• 제49권4호
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• pp.821-831
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• 2022

American plum line pattern virus (APLPV), a member of the genus Ilarvirus in the family Bromoviridae, is one of the plant quarantine pathogens in Korea. In this study, 15 candidate primer sets were designed and examined to develop a reverse transcription polymerase chain reaction (RT-PCR) assay for plant quarantine inspection of APLPV. Using APLPV-infected and healthy samples, the primer sets were assessed for APLPV detection. To confirm the occurrence of nonspecific reactions, six ilarviruses (Apple mosaic virus, Asparagus virus 2, Blueberry shock virus, Prune dwarf virus, Prunus necrotic ringspot virus, and Tobacco streak virus) and 10 target plants (Prunus mume, P. yedoensis, P. persica, P. armeniaca, P. dulcis, P. tomentosa, P. avium, P. glandulosa, P. salicina, and P. cerasifera) were examined. Finally, two primer sets were selected. These primer sets could generate the expected amplicons even with at least 1 ng of the total RNA template in concentration-dependent amplifications. In addition, a positive clone was developed for use as a positive control in the abovementioned RT-PCR assay.

• Journal of Yeungnam Medical Science
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• 제39권2호
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• pp.124-132
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• 2022

Background: Despite recent advances in first-line chemotherapy for advanced pancreatic cancer, standard treatment after the failure of initial chemotherapy has not been established. Hence, we aimed to retrospectively analyze the clinical characteristics and outcomes of second-line chemotherapy in patients with advanced pancreatic cancer. Methods: We reviewed the clinical data of patients with advanced pancreatic cancer who underwent palliative chemotherapy at Kosin University Gospel Hospital between January 2013 and October 2020. Results: Among 366 patients with advanced pancreatic cancer who had received palliative chemotherapy, 104 (28.4%) underwent at least one cycle of second-line chemotherapy. The median age of the patients at the time of initiating second-line treatment was 62 years (interquartile range, 57-62 years), and 58.7% (61 patients) of them were male. The common second-line chemotherapy regimens were 5-fluorouracil (FU) plus leucovorin, irinotecan, and oxaliplatin (33 patients, 31.7%); gemcitabine/nab-paclitaxel (29, 27.9%), gemcitabine±erlotinib (13, 12.5%); and oxaliplatin and 5-FU/leucovorin (12, 11.5%). The median overall survival (OS) and progression-free survival were 6.4 months (95% confidence interval [CI], 4.5-8.6 months) and 4.5 months (95% CI, 2.7-6.3 months), respectively. In a multivariate analysis, poor performance status (PS) (hazard ratio [HR], 2.247; p=0.021), metastatic disease (HR, 2.745; p=0.011), and elevated carcinoembryonic antigen (CEA) levels (HR, 1.939; p=0.030) at the beginning of second-line chemotherapy were associated with poor OS. Conclusion: The survival outcome of second-line chemotherapy for advanced pancreatic cancer remains poor. However, PS, disease extent (locally advanced or metastatic), and CEA level may help determine patients who could benefit from second-line treatment.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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• pp.164-167
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• 1996

This paper presents an off-line P.I.D parameter estimation method during normal operation in power plant. The process parameters are estimated using the recursive least square method. The controller parameters are estimated on the basis of desired characteristics of the dynamic model of the closed-loop control.

• 전자공학회논문지A
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• 제30A권7호
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• pp.19-25
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• 1993

By using the point matching method, the coplanar waveguide shielded by the rectangular waveguide has been analyzed. The particular potential solution of C.P.W has been obtained from the boundary condition by using the point matching method. The line capacitance has been obtained from the total charge of the center conductor per length. The effective dielectric constant and the line impedance have been obtained from the line capacitance of C.P.W.

• 복식문화연구
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• 제4권3호
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• pp.373-381
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• 1996

Draping design system organized by European about 13C has been developed greatly for a long while, but for the purpose of the practical use the Dress form similar to human body has been needed. In order to make three-dimensional effect as fitting Muslin ot the Dress form. the Basic lien has to be established in Muslin and Dress form each. At this time, Shoulder-blade level is indicated from various angles : measure down 10cm from the back neck point, a quarter of the back neck point to the waist lie, the half-way point between neckline and bust line, measure down 41 inches from the back neck line, measure down 3 inches from the top edge of the muslin at center back and cross mark for neck line, and so forth. This study established the Basic line through the two ways of them (B.N.P∼B.L/2, B.N.P∼W.L/4), did tight fitting to the 5 kinds of Dress forms which ar normal type in the drop value(the difference between hip circumference and bust circumference), and acquired Basic Pattern. After the experiment, fitness are throughly investigate by statistical analysis of measurements. As a result, this study finds out that fit is proper when shoulder blade level is situated on a fourth of the back neck point to the waist line and a quarter point between back neck point and waist line, and waist dart is situated on the back center line and princess line.

• 한국동물학회지
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• 제38권2호
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• pp.177-185
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• 1995

두 지역에서 채집한 초파리 자연집단에 대하여 난소발생이상 실험에 의한 P 인자 활성과 세포질형을 분석하여 P 전이인자의 계통형을 조사하였다 전체 238 isofemale line을 조사한 결과 strong P와 true M은 존재하지 않았고, 0(weak P)와 M'(pseudo M) strain이 전체의 98.74%를 차지하여 가장 우세하게 분포하고 있었다. P$\pi$25.1 probe를 이용한 in situ hybridization을 행하여 P 전이인자의 copy수를 조사한 결과 평균 42.12개로 나타났으며, 0와 M'의 계통형 간에 유의적인 차이는 없었다 그러나 염색체 firm당 COPy수는 X염색체가 상염색체의 좌 우 각 arm보다 다소 높게 분포하고 있었고. 염색체상 P 전이인자의 삽입부위에 대한 특이적 좌위는 존재하지 않았다 P 전이인자의 분자구조에 대한 변이형을 조사하기 위하여 southern blot hybridization을 행한 결과 2.9kb의 완전한 크기의 분자를 포함하여 여러종류의 단편들이 확인되었다 조사한 모든 isofemale line에서 KP(1. 15kb)인자를 포함하고 있었으며 이들 KP인자가 P-M System의 난소발생이상을 표현하는데 있어 억제적 작용을 하는 것으로 판단되었다.

• 산업융합연구
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• 제19권6호
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• pp.187-195
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• 2021

본 연구는 여성노인의 라인댄스 프로그램 참여에 따른 면역기능 및 염증변화의 특징을 규명하고자 수행되었다. 이를 위해, Y시에 거주하고 있는 60-70세 여성노인 18명을 선정하였으며, 12주간 라인댄스 프로그램에 참여한 운동군 9명과 동일한 기간 특별한 신체활동에 참여하지 않고 일상적인 생활을 해온 통제군 9명의 두 집단으로 무작위 선정하여 분류하였다. 대상자들의 면역기능 및 염증을 측정하여 자료를 수집하였으며, 자료분석은 SPSS 24.0을 이용하여 평균과 이원변량분석을 실시하였다. 그 결과 면역글로블린인 IgA은 증가하였으나 유의한 증가를 보이지 않았고 IgG(p<.05), IgM(p<.05)은 라인댄스 운동군에서 유의한 증가가 있었으나 통제군에서는 유의한 차이가 나타나지 않았다. 염증지표인CRP(p<.05)은 라인댄스 운동군에서 유의하게 감소하였고, 통제군에서는 유의한 차이가 없었다. 따라서 규칙적인 라인댄스 프로그램은 여성노인의 면역 향상 및 염증 감소에 매우 효과적인 운동프로그램이라 할 수 있으며, 염증을 감소시키고 면역기능 개선에 도움을 줄 수 있음을 보여주는 결과로, 향후 여성노인들의 건강유지와 만성질환 예방을 위해 적극적인 신체활동 프로그램 개발과 중재의 필요성을 시사한다.

• 한국의류학회지
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• 제20권6호
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• pp.1193-1202
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• 1996

This study investigates and analyzes the amount and shape of the front waist dart of the bodice and investigates the dart division method by bust size. Measurements at 5 arm positions were taken from 15 subjects. Clothing tests were given to 6 of the subjects. The conclusions were as follows: 1) Front Body surface changes were occurred around armpit and they were caused by the wrinkles of the armpit 2) The curved dart line was more appropriate for use with C cup, the large bust body, than the linear line. The changes of the dart amount at arm movements had different types by bust size. 3) Patterns for A & C cup body were adequate to divide the dart amount by 3 darts such as under the B.P., under the anterior armpit and side line, or to aggregate to 1 dart under the B.P. For the B cup body, however, the suitable pattern to use was the total dart amount divided by 1~2 darts and decreasing the waist line at the side line should be avoided.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 E
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• pp.1810-1812
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• 1997

This paper describes the results of ground fault analysis on HVDC Transmission Line using TNA (Transient Network Analyzer). The maximum overvoltage is about 1.7 p.u. in the case of single line to ground fault on the overhead transmission line. When the cable is linked to the end of the overhead transmission line, the maximum overvoltage is about 1.58 p.u..