• 한국동물학회지
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• 제38권3호
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• pp.340-347
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• 1995

D. melanogaster를 대상으로 두 종류의 P element construct, 즉 Pc[(ry+)B]와 p[(ry+)$\Delta$SX9]을 사용한 형질전환 실험을 통하여 자율적 및 비자율적인 P element construct간의 vector로써의 효율성을 분석했다. 자율적인 P element 내에 rosy 유전자를 포함하고 있는 Pc[(ry+)B] construct를 진정 M 계통형인 ry506 돌연변이 계통에 주입시켰다. 또한 비자율적인 P element 내에 rosy 유전자를 포함하고 있는 p[(ry+)$\Delta$SX9] construct와 전이효소의 공급원으로써 p-$\Delta$2-3hs$\pi$ helper plasmid를 역시 같은 계통의 ry506 돌연변이 계통에 주입시켰다. Dechorination 방법에 의해 총 1143 embryo를 대상으로 microinjection 한 결과, 모두 35 계통의 형질전환된 정상형의 눈을 가진 계통을 얻었다. P element construct를 주입시킨 전체 1143 embryo 중에서 약 20% 정도가 성체로 부화되었으며, 부화된 개체 가운데 약 40% 정도가 눈 색깔이 거의 정상형과 같은 G0 transformant 로 나타났다. 그러나 생식력을 가진 G0 transformant의 약 40% 만이 G1 transformant로 나타남으로써 모두 35 계통의 transformant를 얻었다. 따라서 G0 단계에서 나타난 형질전환 현상에는 반드시 rosy transformant의 염색체 삽입에 의해서 만이 아니라 세포질내에서도 construct내의 rosy 유전자의 발현이 가능한 것으로 분석되었다. 또한 형질전환율에 있어서는 Pc[(ry+)B]와p[(ry+)$\Delta$SX9] construct 간에 유의한 차가 없는 것으로 나타났다. 이상의 결과로 볼 때, 실험 목적에 따라 자율적인 P element 또는 helper DNA를 이용한 비자율적인 P element를 효과적인 vector 로 선택 이용함으로써 특정 유전자를 곤충집단내로 침투 및 고정시킬 수 있다고 판단된다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2009년 춘계학술대회논문집
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• pp.245-251
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• 2009

Numerical algorithms for solving the incompressible Navier-Stokes equations using P2P1 finite element are compared regarding the eigenvalues of matrices. P2P1 element allocates pressure at vertex nodes and velocity at both vertex and mid nodes. Therefore, compared to the P1P1 element, the number of pressure variables in the P2P1 element decreases to 1/4 in the case of two-dimensional problems and to 1/8 in the three-dimensional problems. Fully-implicit-integrated, semi-implicit- integrated and semi-segregated finite element formulations using P2P1 element are compared in terms of elapsed time, accuracy and eigenvlue distribution (condition number). For the comparison,they have been applied to the well-known benchmark problems. That is, the two-dimensional unsteady flows around a fixed circular cylinder and decaying vortex flow are adopted to check spatial accuracy.

• 대한기계학회논문집B
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• 제33권4호
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• pp.242-247
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• 2009

AILU type preconditioners for a two-dimensional combined P2P1 finite element formulation of the interaction of rigid cylinder with incompressible fluid flow have been devised and tested by solving fluid-structure interaction (FSI) problems. The FSI code simulating the interaction of a rigid cylinder with an unsteady flow is based on P2P1 mixed finite element formulation coupled with combined formulation. Four different preconditioners were devised for the two-dimensional combined P2P1 finite element formulation extending the idea of Nam et al., which was proposed for the preconditioning of a P2P1 mixed finite element formulation of the incompressible Navier-Stokes equations. It was found that PC-III or PC-IV among them perform well with respect to computational memory and convergence rate for some bench-mark problems.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 춘계학술대회논문집A
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• pp.345-350
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• 2000

Adaptive finite element analysis, which its solution error meets with the user defined allowable error, is recently used far improving reliability of finite element analysis results. This adaptive analysis is composed of two procedures; one is the error estimation of an analysis result and another is the reconstruction of finite elements. In the rp-method, an element size is controlled by relocating of nodal positions(r-method) and the order of an element shape function is determined by the hierarchical polynomial(p-method) corresponding to the element solution error. In order to show the effectiveness and accuracy of the suggested rp-method, various numerical examples were analyzed and these analysis results were examined by comparing with those obtained by the existed methods. As a result of this study, following conclusions are obtained. (1) rp-method is more accurate and effective than the r- and p-method. (2) The solution convergency of the rp-method is controlled by means of the iterative calculation numbers of the r- and p- method each other.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제4권1호
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• pp.67-77
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• 2000

In this paper we consider finite element mathods for nonlinear parabolic problems defined in ${\Omega}{\subset}R^d$ ($d{\leq}4$). A new initial approximation is taken. Optimal order error estimates in $L_p$ for $2{\leq}p{\leq}{\infty}$ are established for arbitrary order finite element. One order superconvergence in $W^{1,p}$ for $2{\leq}q{\leq}{\infty}$ are demonstrated as well.

• Structural Engineering and Mechanics
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• 제22권2호
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• pp.151-167
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• 2006

A p-version of the finite element method in conjunction with the modeling dynamic method using the arc-length stretch deformation is considered to determine the bending natural frequencies of a cantilever flexible plate mounted on the periphery of a rotating hub. The plate Fourier p-element is used to set up the linear equations of motion. The transverse displacements are formulated in terms of cubic polynomials functions used generally in FEM plus a variable number of trigonometric shapes functions representing the internals DOF for the plate element. Trigonometric enriched stiffness, mass and centrifugal stiffness matrices are derived using symbolic computation. The convergence properties of the rotating plate Fourier p-element proposed and the results are in good agreement with the work of other investigators. From the results of the computation, the influences of rotating speed, aspect ratio, Poisson's ratio and the hub radius on the natural frequencies are investigated.

• 한국공간구조학회논문집
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• 제8권4호
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• pp.81-90
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• 2008

이 논문에서는 적분형 르장드르 다항식을 사용한 3차원 계층적 고체요소의 유한요소 정식화를 보여준다. 제안하는 육면체 고체요소는 절점, 변, 면, 그리고 내부모우드를 포함한은 4개의 서로 다른 모우드로 구성되어 있다. 영에너지 모우드와 일정변형률 조건을 확인하기 위해 고유치 시험과 조각시험이 수행되었다. 여기에 추가되어, 적응적 p-유한요소해석을 위해 유한요소해석으로부터 구한 후처리 응력값의 평활화에 기초를 둔 사후오차평가 기법이 연구된다. 자유도가 증가함에 따라 수렴속도측면에서 균등 p-분배와 불균등 p-분배에 의한 유한요소해의 차이점이 비교된다. 제안된 요소의 성능을 보이기 위해 간단한 캔틸레버보가 테스트되었다.

• BMB Reports
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• 제32권1호
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• pp.45-50
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• 1999

We previously found three transcription factor-binding motifs in the rat p53 promoter. They are two recognition motifs of NF1-like protein (NF1-like element 1: -296 ~ -312, NF1-like element 2: -195 ~ -219) and a bHLH protein binding element (-142 ~ -146). In this study, we investigated the DNA-protein complex formation of the three elements with nuclear extracts from both normal and regenerating liver to find the element involved in the induced transcription of p53. The level of each DNA-protein complex on NF1-like and bHLH motifs was not changed. Instead, a new element located at -264 ~ -284 was detected in the DNase I footprinting assay with regenerating nuclear extract. This element has partial homology to the AP1 consensus motif. However, the competition studies with diverse oligonucleotides suggest that the binding protein is not AP1. An in vitro transcription assay shows that this element is important for the transcriptional activation of the rat p53 promoter. Therefore, for the induced transcription of the rat p53 promoter, the-264 ~ -284 region is required in addition to two NF1-like and one bHLH motif.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집E
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• pp.386-391
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• 2001

A finite element code based on P2P1 tetra element has been developed for the large eddy simulation (LES) of turbulent flows around a complex geometry. Fractional 4-step algorithm is employed to obtain time accurate solution since it is less expensive than the integrated formulation, in which the velocity and pressure fields are solved at the same time. Crank-Nicolson method is used for second order temporal discretization and Galerkin method is adopted for spatial discretization. For very high Reynolds number flows, which would require a formidable number of nodes to resolve the flow field, SUPG (Streamline Upwind Petrov-Galerkin) method is applied to the quadratic interpolation function for velocity variables, Noting that the calculation of intrinsic time scale is very complicated when using SUPG for quadratic tetra element of velocity variables, the present study uses a unique intrinsic time scale proposed by Codina et al. since it makes the present three-dimensional unstructured code much simpler in terms of implementing SUPG. In order to see the effect of numerical diffusion caused by using an upwind scheme (SUPG), those obtained from P2P1 Galerkin method and P2P1 Petrov-Galerkin approach are compared for the flow around a sphere at some Reynolds number. Smagorinsky model is adopted as subgrid scale models in the context of P2P1 finite element method. As a benchmark problem for code validation, turbulent flows around a sphere and a MIRA model have been studied at various Reynolds numbers.

• 한국지반공학회지:지반
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• 제13권1호
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• pp.101-110
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• 1997

본 논문에서는 무한영역에서 발생하는 임의의 1$1/r^n$변위감쇠특성을 해석할 수 있는 p-버전 정적 무한요소를 연구하였다. 무한요소를 개발하기 위하여 이론해를 근사화한 형상함수를 사용하였다. 균질한 무한 탄성체내의 공동변형문제와 반무한탄성체위에 놓인 강성기초의 거동해석을 통하여, 본 연구에서 개발된 무한요소가 무한영역을 효율적으로 묘사할 수 있음을 검토하였다.