• Title/Summary/Keyword: P-S-N 선도

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Cloning of nif Gene Cluster from Klebsiella pneumoniae and Expression in Escherichia coli (Klebsiella pneumoniae의 nif Gene Cluster의 Cloning 및 Escherichia coli 에서의 발현)

  • 이재선;이성희;심웅섭
    • Korean Journal of Microbiology
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    • v.27 no.1
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    • pp.21-26
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    • 1989
  • The chromosomal DNA of Klebsiella penumoniae KNF1 was partially digested with HindIII. pBR322 was completely digested with same enzyme with additional alkaline phosphatase treatment. Both DNA samples were ligated and transformed into E. coli KO60. Single coloneis of the transformed cells were isolated on N0free agar media. These colonies were ampicillin-resistant and tetracycline-sensitive. When the plasmids in transformants were cured, nitrogen-fixing activities were lost. Therefore, these transformants harbored recombinant plasmid including nif genes of K. pneumoniae. Nitrogenase activity of transformant was higher than or the same as that of K. pneumoniae KNF1.

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Investigation of Various Radiation Proton Energy Effect on n, p Type Silicon by Positron Annihilation Method (양전자 소멸 측정법으로 양성자 조사에너지 변화에 대한 n, p형 실리콘 구조 특성)

  • Lee, Chong Yong
    • Journal of the Korean Vacuum Society
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    • v.22 no.6
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    • pp.341-347
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    • 2013
  • The n-type and p-type silicon samples were exposed by 40.0, 3.98 MeV proton beams ranging between 0 to $20.0{\times}10^{13}protons/cm^2$. Coincidence Doppler Broadening Positron Annihilation Spectroscopy (CDBPAS) were applied to study of defect characteristics of p type and n type silicon samples. In this investigation the numerical analysis of the spectra was employed to the determination of the shape parameter, S, defined as the ratio between the amount of counts in a central portion of the gamma spectrum and the total counts of whole gamma spectrum. The S-parameter values strongly depend on the irradiated proton beam that indicated the defects generate more, rather than the energy intensity. 40 MeV irradiated proton beam in the n-type silicon at $20.0{\times}10^{13}protons/cm^2$ was larger defects than 3.98 MeV irradiated proton beam. It was analysis between the proton irradiation beams and the proton intensities of the irradiation. Because of the Bragg peak, SRIM results shows mainly in a certain depth of the sample to form the defect by the proton irradiation, rather than the defects to appear for the entire sample.

Synthesis and Structure of N-Methylphenazinium-Tetracynnopalladate(II) Hydrate (N-Methylphenazinium-Tetracyanopalladate(II) Hydrate의 합성 및 결정구조 연구)

  • NamGung, Hae;Lee, Hyun-Mi
    • Korean Journal of Crystallography
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    • v.17 no.1
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    • pp.6-9
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    • 2006
  • Crystal structure of Bis(N-Methylphenazinium)-Tetracyanopalladate(II) hydrate has been determined by X-ray crystallography. Crystal data: $(C_{13}H_{11}N_2){_2}[Pd(cn)_4]{\cdot}H_2O$, Monocline, Space group $P2_1/b$(No=14), a=9.783(4), b=10.788(4), c=13.666(4) ${\AA},\;{\beta}=104.59(5),\;Z=2,\;V=1392.9{\AA}{^3},\;Dc=1.476gcm^{-3},\;F(000)=632,\;{\mu}=7.05cm^{-1}$. The structure was solved by Patterson method and refined by full matrix least-square methods using unit weights. The final R and S values were R=0.0257, Rw=0.0732, Rall=0.0283 and S=1.07 for 1930 observed reflections. Both cation and anion complexes are essentially planar and have dihedral angles of $10.16(4)^{\circ}$. The planar complex anions are sandwiched between slightly bent cations. The interplanar separations in one triad and between two triads are 3.419(3) and $3.402(4){\AA}$, respectively. The triads are stacked along b-axis.

Purification and Characterization of a Fibrinolytic Enzyme Produced by Bacillus sp. S19 from Shrimp (새우젓 유래 Bacillus sp. S19가 생산하는 혈전용해 요소의 정제 및 특성)

  • 장순애;김명희;이명선;오태광;손천배
    • Microbiology and Biotechnology Letters
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    • v.28 no.5
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    • pp.258-263
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    • 2000
  • A fibrinolytic enzyme was purified to homogeneity from Bacillus sp. S19 using DEAE and CM column chromatograhies, and gel filtration with a recovery yield of 13%. Its molecular mass was estimated to be 42 kDa by SDS-PAGE. The pH and temperature optima were 8.0 and $40^{\circ}C$, respectively. The enzyme was stable up to $45^{\circ}C$ and over a pH range of 6-9. The N-terminal amino acid sequence of the enzyme was determined as Alsa-Gln-Asp-Ala-Thr-Val-Asn-Ile-Ser-Ala-Glu-Arg-Gln-Val-Ile. The fibrinolytic activity was increased by $Cu^{2+}$ while it was strongly inhibited by metal ions such as $Cd^{2+}$ and $Ba^{2+}$ . In addition, the enzyme was inhibited by EDTA, but not by PMSF, suggesting that it is a metallorprotease.

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Effects of Composted Pig Manure Application on Enzyme Activities and Microbial Biomass of Soil under Chinese Cabbage Cultivation (돈분퇴비의 시용이 토양의 미생물체량 및 효소활성에 미치는 영향)

  • Weon, Hang-Yeon;Kwon, Jang-Sik;Shin, Yong-Kwang;Kim, Seung-Hwan;Suh, Jang-Sun;Choi, Woo-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.2
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    • pp.109-115
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    • 2004
  • To elucidate the effects of composted pig manure on soil biochemical properties, composted pig manure was amended in a sandy loam soil and Chinese cabbage was grown. Composted pig manure treatments included 8, 29 and $57Mg\;ha^{-1}$ for CM-08, CM-29, and CM-57 plots, respectively. Biomass contents and enzymes activities in the non-rhizophere soil were measured. Activities of protease, phosphomonoesterase and dehydrogenase in the plot CM-57 increased to 2.3, 1.6, and 2.4 fold as compared with those of the control plot. Soil microbial biomass contents increased in proportion to the application rates of compost and biomass C, N, and P in the plot CM-59 were 4.3, 3.4, 2.8-fold higher than those of control p1ot(no fertilizer), respectively. During cultivation of Chinese cabbage, biomass C and N were higher in the middle growth stage, although biomass P was the highest in the early growth stage. The average ratio of biomass C:N:P was 11:2:1, and proportion of biomass C and N in the soil organic C and N was 1.1 and 3.6%, respectively. Activities of protease and dehydrogenase had significant correlations with biomass C and P.

전자선 직접묘사에 의한 Deep Submicron $p^+$Poly pMOSFET 제작 및 특성

  • Kim, Cheon-Su;Lee, Jin-Ho;Yun, Chang-Ju;Choi, Sang-Soo;Kim, Dae-Yong
    • ETRI Journal
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    • v.14 no.1
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    • pp.40-51
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    • 1992
  • $0.25{\mu} m$ 급 pMOSFET소자를 구현하기 위해, $P^+$ 폴리실리콘을 적용한 pMOS를 제작하였으며, $p^+$ 폴리실리콘 게이트 소자에서 심각하게 문제가 되고 있는 붕소이온 침투현상을 조사하고 붕소이온 침투가 일어나지 않는 최적열처리온도를 조사하였다. 소자제조 공정중 게이트 공정만 전자선 (EBML300)을 이용하여 직접묘사하고 그 이외의 공정은 stepper(gline) 을 사용하는 Mix & Match 방법을 사용하였다. 또한 붕소이온 침투현상을 억제하기 위한 한가지 예로서, 실리콘산화막과 실리콘질화막을 적층한 ONO(Oxide/Nitride/Oxide) 구조를 게이트 유전체로 적용한 소자를 제작하여 그 가능성을 조사하였다. 그 결과 $850^{\circ}C$의 온도와 $N_2$ 분위기에서 30분동안 열처리 하였을 경우, 붕소이온의 침투현상이 일어나지 않음을 SIMS(Secondary Ion Mass Spectrometer) 분석 및 C-V(Capacitance-Voltage) 측정으로 확인할 수 있었으며 그 이상의 온도에서는 붕소이온이 침투되어 flat band전압(Vfb)을 변화시킴을 알았다. 6nm의 얇은 게이트 산화막 및 $0.1{\mu} m$ 이하의 LDD(Lightly Doped Drain) $p^-$의 얇은 접합을 형성함으로써 소자의 채널길이가 $0.2 {\mu} m$까지 짧은 채널효과가 거의 없는 소자제작이 가능하였으며, 전류구동능력은 $0.26\muA$/$\mu$m(L=0.2$\mu$m, V$_DS$=2.5V)이었고, subthreshold 기울기는 89-85mV/dec.를 얻었다. 붕소이온의 침투현상을 억제하기 위한 한가지 방법으로 ONO 유전체를 소자에 적용한 결과, $900^{\circ}C$에서 30분의 열처리조건에서도 붕소이온 침투현상이 일어나지 않음으로 미루어 , $SiO_2$ 게이트 유전체보다 ONO 게이트 유전체가 boron 침투에 대해서 좋은 장벽 역활을 함을 알았다. ONO 게이트 유전체를 적용한 소자의 경우, subthreshold특성은 84mV/dec로서 좋은 turn on,off 특성을 얻었으나, ONO 게이트 유전체는 막자체의 누설전류와 실리콘과 유전체 계면의 고정전하량인 Qss의 양이 공정조건에 따라 변화가 심해서 문턱전압 조절이 어려워 소자적용시 문제가 된다. 최근 바닥 산화막(bottom oxide) 두께가 최적화된 ONO 게이트 유전체에 대하 연구가 활발히 진행됨을 미루어, 바닥 산화막 최적화가 된다면 더 좋은 결과가 예상된다.

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Crystal Structure of Probenecid, $C_{13}H_{19}NO_4S$ (Probenecid, $C_{13}H_{19}NO_4S$의 結晶構造)

  • Kim, Eui-Sung;Shin, Hyun-So
    • Journal of the Korean Chemical Society
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    • v.35 no.6
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    • pp.603-606
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    • 1991
  • The crystal structure of Probenecid has been determined from 2574 independent reflections collected on an automatic ENRAF-NONIUS CAD-4 diffractometer using graphite-monochromated $Mo-K{\alpa}$ radiation. The crystal is triclinic, space group P$\bar{1}$ with unit cell dimensions a = 7.535(2)${\AA}$, b = 18.473 (5)${\AA}$, c = 5.317(9)${\AA}$, ${\alpha} = 92.00(5)^{\circ}$, ${\beta} = 99.02(5)^{\circ}$, ${\gamma} = 94.89(2)^{\circ}$, V = 727.4(2)${\AA}^3$, Z = 2, $D_m$ = 1.310, $D_x$ = $1.302 gcm^{-3}$, ${\mu}$ = $1.88 cm^{-1}$, F(000) = 304, and T = 298 K. Final R = 0.0676 and $R_w$ = O.0630 for 1209 reflections > 5${\sigma}(F_o)$. In the spacial arrangement about N(13), the sum of bond angles about nitrogen is 350.9° and the nitrogen lies only 0.268(6)${\AA}$ out of S(1)-C(14)-C(17) plane. The S(1)-C(4) distance is 1.792(6)${\AA}$ and the C(4)-S(1)-N(13) angle is $106.5(3)^{\circ}$. The overall conformation of the molecule is folded with respect to sulfur.

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전세계 타이어제조회사 및 공장별 생산능력현황

  • Lee, Won-Seon
    • The tire
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    • s.125
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    • pp.2-12
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    • 1986
  • 본자료는 Rubber & Plastics News 유럽 통신원 Bruce Davis와 R.P.N. 특종기사편집자 Chuck Slaybaugh에 의해 조사된, 전세계 타이어 제조회사 현황과 회사별 타이어공장소재지 및 공장별 생산능력 등을 개괄적으로 요약표시한 것이다. 그리고 본조사에서는 전세계를 7개지역, 즉 북미(미국,캐나다), 중남미, 서구(핀랜드포함), 동구 및 소련, 아시아,중동 및 아프리카, 대양주 등으로 나누어서 각 지역별 타이어 생산공장의 분석현황을 지도상에 표시하여 일목요연하게 알 수 있도록 하였다. 특히, 이번 조사에서는 각지역 전반에 걸쳐 잘 알려지지 않았던 타이어공장들까지도 자세히 조사하여 수록하였다.

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The Crystallograpic and Magnetic Properties of EuFeO3 Doped with in ions (In 이온을 첨가한 $EuFeO_{3}$의 결정구조 및 자기적 성질)

  • 김정기;서정철;한은주
    • Journal of the Korean Magnetics Society
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    • v.4 no.4
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    • pp.335-339
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    • 1994
  • The crystallograpic and magnetic properties of $Eu(Fe_{1-x}In_{x})O_{3}$ (x=0, 0.03과 0.05) have been studied by the methods of X-ray diffraction, $M\"{o}ssbauer$ spectroscopy, and magnetic hysteresis measurement at room temperature. The X-ray results show that the samples have a crystal structure of orthorhombic and unit cell volume of the crystal with the exception of the sample of x=0 increases as increasing the In concentration. In the analysis assuming two sets of six-line of $M\"{o}ssbauer$ spectra, it is found that the magnetic hyperfine field in each of sets decreases increasing x. The linewidth of the absorption lines for the samples increased as increasing x. This implies that the data involve a sum of several hyperfine patterns which have intensity being proportional to $_{n}P_{z}(x)$, the probability of an environment with z such Fe neighbors. The magnetic hysteresis curves show decrease of $M_{s}$ and increase of $H_{c}$ of the samples with increasing x.

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The Crystal Structure of Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II) (Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II)의 결정구조)

  • Go, Gi-Yeong;Nam, Gung-Hae;Han, Sang-Gon
    • Korean Journal of Crystallography
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    • v.9 no.1
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    • pp.71-76
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    • 1998
  • Crystal structure of Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II0 has been determined by X-ray crystallography. Crystal data : (Pd(C2H8N2)2.Pd(C2O4)2), Fw=509.04, Monocline, Space Group P21/c (no=14), a=6.959(2), b=13.506(2), c=15.339(2) Å, β=99.94(3), Z=4, V=1420 Å3, Dc=2.380 gcm-3, μ=25.46cm-1, F(000)=992. The intensity data were collected with Mo-Kα radiation (λ=0.7107 Å) on an automatic four-circle diffractometer with a graphite monochromater. The structure was solved by Patterson method and refined by full matrix least-square methods using unit weights. The final R and S values were R=0.021, Rw=0.030, Rall=0.032 abd S=2.1 for 1472 observed reflections. The essentially planar complex anions form diade of interplanar distances of 3.41 Å and their diads are stacked along aaxis with interplanar separation of 3.44 Å.

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