• BMB Reports
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• v.29 no.4
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• pp.353-358
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• 1996

Bovine angiogenin (bAng) is a potent blood vessel inducing protein purified from cow In ilk. fluorescence spectroscopy has been used to study the interaction of bAng with actin in 50 mM Tris-HCl pH 7.5, and 1 mM $CaCl_2$ at $25^{\circ}C$. Actin contains four tryptophans but bAng contains no tryptophans. A 50% decrease in intrinsic fluorescence accompanied formation of the bAng/actin complex. By contrast, the interaction of RNase A, a homologous protein to bAng, with actin results in about 10% quenching of the fluorescence. Fluorescence titration experiments were performed by adding increasing concentrations of bAng (0~1.0 ${\mu}M$) to a constant concentration of actin (0.1 ${\mu}M$), and the dissociation constant $K_d$ for the bAng/actin complex and the stoichiometry n were measured as $20{\pm}1$ nM and $1.0{\pm}0.1$ respectively. These results suggest that the interaction between bAng with actin is specific and that quenching of actin fluorescence has occurred in the bAng/actin complex. The bAng binding sites of actin are discussed in the results of this study, and we propose that Trp-80 in the small domain of bovine actin is responsible for the bAng/actin binding.

• Proceedings of the Korean Biophysical Society Conference
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• 1996.07a
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• pp.43-43
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• 1996

Bovine angiogenin (bAng) is a potent blood vessel inducing protein purified from cow milk. Fluorescence spectroscopy has been used to study the interaction of bAng with actin in 50 mM Tris-HCl, pH 7.5, and 1 mM CaCl$_2$ at 25$^{\circ}C$. Actin contains four tryptophans but bAng contains no tryptophans. A 50% decrease in intrinsic fluorescence accompanied formation of the bAng/actin complex. (omitted)

• Bulletin of the Korean Chemical Society
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• v.23 no.8
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• pp.1073-1077
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• 2002

Binding of dodecyl trimethylammonium bromide (DTAB) to human and bovine hemoglobin and globin samples has been investigated in 50 mM glycine buffer pH = 10, I = 0.0318 and 300 K by equilibrium dialysis and temperature scanning spectrophotometry techniques and method for calculation of average hydrophobicity. The binding data has been analyzed, in terms of binding capacity concept $({\theta})$, Hill coefficient (nH) and intrinsic Gibbs free energy of binding $({\Delta}Gbv).$ The results of binding data, melting point (Tm) and average hydrophobicity show that human hemoglobin has more structural stability than bovine hemoglobin sample. Moreover the results of binding data analysis represent the systems with two and one sets of binding sites for hemoglobin and globin, respectively. It seems that the destabilization of hemoglobin structure due to removal of heme group, is responsible of such behavior. The results indicating the removal of heme group from hemoglobin caused the depletion of first binding set as an electrostatic site upon interaction with DTAB and exposing the hydrophobic patches for protein.

• Proceedings of the Korean Biophysical Society Conference
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• 1999.06a
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• pp.44-44
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• 1999

Yeast cytochrome c peroxidase (CcP) was cloned and overexpressed in E. coli, and purified by a Ni$^{2＋}$-affinity column. HoloCcP was obtained by reconstituting apoCcP with Mn$^{3+}$-protoporphyrin IX (MnPP). Electron paramagnetic resonance (EPR) spectra of spin-labeled holoCcP showed a slightly more immobilized signal than spin-labeled apoCcP.(omitted)

• The Journal of Korean Physical Therapy
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• v.29 no.5
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• pp.255-258
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• 2017

Purpose: This study aimed to investigate the effects of compelled weight shift in paretic lower limb training on balance ability in patients with stroke. Methods: Thirty-six individuals with hemiparesis, who were randomly assigned to a 10CWST (10 mm constrained -weight shift training) group, a 5CWST (5 mm constrained-weight shift training) group, and a control group participated in this study. Compelled weight shift training was performed in 3 sets of 5 min with a rest period of 1 min between sets. Both the 5CWST and 10CWST groups performed 5 times per week for 4 weeks. Static (mediolateral and anteroposterior sway velocities) and dynamic balance (mediolateral and anteroposterior distances) was assessed using the Good Balance system. Results: Significant differences were found in the M-L and A-P sway velocities, and the M-L sway distance. The M-L and A-P sway velocities, and M-L sway distance showed significantly large group effects (p<0.05), time effects (p<0.05), and group-by-time interaction (p<0.05). The post hoc analyses indicated that, following intervention, the 10CWST group showed more significant changes in the M-L and A-P sway velocities, and the M-L sway distance than the control group. Conclusion: These results suggest that the use of compelled weight shift in paretic lower limb training may be an effective method to improve balance ability in patients with stroke.

• Nutrition Research and Practice
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• v.12 no.1
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• pp.61-68
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• 2018

BACKGROUND/OBJECTIVES: This study aimed to test the association between APOA5 3'-UTR variants (rs662799) and cardiometabolic traits in Koreans. SUBJECTS/METHODS: For this study, epidemiological data, Apolipoprotein A5 (APOA5) genotype information, and lymphoblastoid cell line (LCL) biospecimens from a subset of the Ansung-Ansan cohort within the Korean Genome and Epidemiology study (KoGES-ASAS; n = 7,704) as well as epidemiological data along with genomic DNA biospecimens of participants from a subset of the Korea National Health and Nutrition Examination Survey (KNHANES 2011-12; n = 2,235) were obtained. APOA5 mRNA expression was also measured. RESULTS: APOA5 rs662799 genotype distributions in both the KoGES-ASAS and KNHANES groups were 50.6% for TT, 41.3% for TC, and 8.1% for CC, which are similar to those in previous reports. In both groups, minor C allele carriers, particularly subjects with CC homozygosity, had lower high-density lipoprotein (HDL) cholesterol and higher triglyceride levels than TT homozygotes. Linear regression analysis showed that the minor C allele significantly contributed to reduction of circulating HDL cholesterol levels [${\beta}=-2.048$, P < 0.001; ${\beta}=-2.199$, P < 0.001] as well as elevation of circulating triglyceride levels [${\beta}=0.053$, P < 0.001; ${\beta}=0.066$, P < 0.001] in both the KoGES-ASAS and KNHANES groups. In addition, higher expression levels of APOA5 in LCLs of 64 healthy individuals were negatively associated with body mass index (r = -0.277, P = 0.027) and circulating triglyceride level (r = -0.340, P = 0.006) but not significantly correlated with circulating HDL cholesterol level. On the other hand, we observed no significant difference in the mRNA level of APOA5 according to APOA5 rs662799 polymorphisms. CONCLUSIONS: The C allele of APOA5 rs662799 was found to be significantly associated with cardiometabolic traits in a large Korean population from the KoGES-ASAS and KNHANES. The effect of this genotype may be associated with post-transcriptional regulation, which deserves further experimental confirmation.

• Journal of Korean Public Health Nursing
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• v.9 no.1
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• pp.1-16
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• 1995

The purpose of this study was to find out and analyze the satisfaction degree related to job by general characteristics, satisfaction factors. This survey was based on the questionnaire method. The sample consisted of 51 noncommissioned officers, 72 administrative officers and 160 nurse officers from army hospitals. The data were collected from March 14 to April 18, 1994 and statistically analysed by percentage, mean and $x^2$ test. Factors of job satisfaction consist of 6 categories; job pristige, interaction, autonomy, task requirements, pay, and organizational requirements. The overall job satisfaction degree was $62.8\%$ in NCOs, $54.2\%$ in administrative officers and $33.3\%$ in nurse officers. The job satisfaction of NCCs increased in factors of job prestige (p<0.01), autonomy (p<0.05), task requirement (p<0.05), and was higher as age. There were statistically significant in the factors of pay in school background, Job prestige in rank (p<0.05). 'I'm OK and You're OK' type was $76.5\%$ which was the highest rate and A major personal traits also showed the highest job satisfaction $(63.7\%)$. There were significant difference between major personal traits and interaction (p<0.01), autonomy (p<0.05), organizational requirement (p<0.01), pay (p<0.05) and task requirement. The job satisfaction of administrative Officers was represented significant differences in factors of pay (p<0.05), task requirements (p<0.05) by school background. Long term Workers showed the highest job satisfaction and significant difference in factors of job prestige and organizational requirement (p<0.01). Job satisfaction in 'I'm OK but you're not OK' type was slightly high, and CP major personal traits also showed the highest job satisfaction. The job satisfaction of nurse officers was represented significant difference in factors of autonomy (p<0.01), job prestige (p<0.05), organizational requirement (p<0.05), and pay by age. In CP major personal traits. job satisfaction showed the highest rate (75.0)

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.3
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• pp.203-212
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• 2020

Promyelocytic leukemia (PML) gene, through alternative splicing of its C-terminal region, generates several PML isoforms that interact with specific partners and perform distinct functions. The PML protein is a tumor suppressor that plays an important role by interacting with various proteins. Herein, we investigated the effect of the PML isoforms on oncostatin M (OSM)-induced signal transducer and activator of transcription-3 (STAT-3) transcriptional activity. PML influenced OSM-induced STAT-3 activity in a cell type-specific manner, which was dependent on the p53 status of the cells but regardless of PML isoform. Interestingly, overexpression of PML exerted opposite effects on OSM-induced STAT-3 activity in p53 wild-type and mutant cells. Specifically, overexpression of PML in the cell lines bearing wild-type p53 (NIH3T3 and U87-MG cells) decreased OSM-induced STAT-3 transcriptional activity, whereas overexpression of PML increased OSM-induced STAT-3 transcriptional activity in mutant p53-bearing cell lines (HEK293T and U251-MG cells). When wild-type p53 cells were co-transfected with PML-IV and R273H-p53 mutant, OSM-mediated STAT-3 transcriptional activity was significantly enhanced, compared to that of cells which were transfected with PML-IV alone; however, when cells bearing mutant p53 were co-transfected with PML-IV and wild-type p53, OSM-induced STAT-3 transcriptional activity was significantly decreased, compared to that of transfected cells with PML-IV alone. In conclusion, PML acts together with wild-type or mutant p53 and influences OSM-mediated STAT-3 activity in a negative or positive manner, resulting in the aberrant activation of STAT-3 in cancer cells bearing mutant p53 probably might occur through the interaction of mutant p53 with PML.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.375-383
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• 2000

A mixed culture isolated from petroleum-contaminated soil was enriched on toluene as a sole carbon and energy source, and degradation characteristics of BTEX(Benzene, Toluene, Ethylbenzene, Xylenes) was observed. In the single-substrate experiments, all the BTEX compounds were degraded, and it was degraded as following orders; toluene, benzene, ethylbenzene, and p-xylene. In the degradation experiments of BTEX mixtures, the degradation rate was decreased compared to that in the single substrate experiment and ethylbenzene was degraded faster than benzene. In the experiments of binary-mixtures, various substrate interactions such as inhibition, stimulation, and non-interaction were observed, and ethylbenzene was shown to be most potent inhibitor of BTEX degradation. In the degradation characteristic studies of xylene isomers, m-xylene and p-xylene were degraded as carbon sources, and it was stimulated in the presence of either benzene or toluene. However, degradation of o-xylene was enhanced only in the presence of benzene.

• The Journal of the Korean dental association
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• v.11 no.3
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• pp.205-209
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• 1973

법랑질과 상아질의 타액단백의 흡착관계를 구명키 위하여 타액을 법랑질과 상아질 분말의 column에 흡착시킨후 증류수, 3M Nacl, 0.1M phosphate buffer, pH 7.1, 0.2M EDTA 함유한 phosphate buffer와 증류수를 차례로 용출시켜 용출액의 amylase, ribonuclease 및 단백양을 관찰한 결과는 다음과 같다. 1. 타액 amylase와 ribonuclease는 법랑질과 상아질 분말에 모두 흡착하였다. 2. 0.2M EDTA가 함유한 buffer에서는 단백양을 법랑질과 상아질분말에서 모두 33.5%정도 용출되었으나 효소활성은 없었다.