Proceedings of the Korean Biophysical Society Conference (한국생물물리학회:학술대회논문집)

The Korean Biophysical Society (한국생물물리학회)
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Interaction of Cytochrome c and $Mn^{2+}$ -Cytochrome c Peroxidase

  • Kim, Mun-kyoung (Division of Biological Science, Kangwon National University) ;
  • M. Kwon (Division of Biological Science, Kangwon National University) ;
  • Kim, K. (Division of Biological Science, Kangwon National University) ;
  • Sanghwa Han (Division of Biological Science, Kangwon National University)
  • Published : 1999.06.01
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Abstract

Yeast cytochrome c peroxidase (CcP) was cloned and overexpressed in E. coli, and purified by a Ni$^{2＋}$-affinity column. HoloCcP was obtained by reconstituting apoCcP with Mn$^{3+}$-protoporphyrin IX (MnPP). Electron paramagnetic resonance (EPR) spectra of spin-labeled holoCcP showed a slightly more immobilized signal than spin-labeled apoCcP.(omitted)

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