• Journal of Mushroom
• /
• v.11 no.3
• /
• pp.131-136
• /
• 2013

The effects of fixed and variable relative humidity on fruiting body formation and characters of Pleurotus eryngii were investigated with normal and thinning treatment plots. In fixed relative humidity, as humidity was lower, period of harvest was longer as well as days for pinheading in the both of normal and thinning plots. In the normal plot, qualities of mushroom were 5.5, 5.8, and 6.3 and yield was 98.6 g per bottle for 90% relative humidity, it was best. In the thinning plot, qualities of mushroom were 7.7, 8.4, and 8.5 and yields were 102.1, 105.8, and 116.9 g at 70, 80, and 90% respectively. In variable relative humidity with a thinning plot, the yield of P. eryngii on condition I(>90% for 1 day ${\rightarrow}$ 85% until thinning(for about 11 days) ${\rightarrow}$ 80%) and III(>90% until pin-heading(about for a week) ${\rightarrow}$ 85% until thinning(about for 5 days) ${\rightarrow}$ 80%) were 85.5 and 87.8 g per bottle, and qualities were 7.8 and 8.0 respectively. For long shelf life and a cultural control of bacterial soft rot disease, the condition I will be more largely adopted by mushroom farmers.

• Research in Plant Disease
• /
• v.9 no.2
• /
• pp.94-98
• /
• 2003

To produce antibodies against Pseudomonas tolaasii and P. agarici, lyophilized P. tolaasii and P. agarici ($5{\times}10^7$ cfu/ml) and Freund, s adjuvant were immunized into rabbits 4 times. The specificity and sensitivity of the antibodies were evaluated by immunodiffusion test and indirect enzyme-linked immunosorbent assay (id ELISA). The ${\alpha}$-P. tolaasii antibody was very specific only against P. tolaasii, while ${\alpha}$-P. agarici antibody was not specific and showed a high cross reactivity toward P. tolaasii with detection limit concentration of $2{\times}10^3$ cfu/ml. However, the cross reactivities of ${\alpha}$-P. agarici antibody toward the related species including P. reactans were very low. Our results showed that ${\alpha}$-P. tolaasii and ${\alpha}$-P. agarici antibodies against P. tolaasii and P. agarici, respectively, might be useful for rapid and simple detection of the causal agents of bacterial brown and yellow blotches in cultivated oyster mushrooms.

• The Korean Journal of Mycology
• /
• v.36 no.1
• /
• pp.58-62
• /
• 2008

'Miso', a new variety of oyster mushroom was developed for the bottle culture at the Chungnam Agricultural Research & Extension Services. Its mycelium grew rapidly with $8.4{\sim}8.6\;mm/day $ at $25{\sim}28^{\circ}C$ on PDA medium. The optimum pH of the mycelial growth was pH 5.0. It took 24 days for the primitive primordium formation after inoculation on pine sawdust media mixed with 20% wheat bran. Fruiting body color was white, and the shape of pileus was convex-umbonate. In the bottle culture, the yield was 115.7 g per 850 ml bottle. Stipe length was about 54 mm, the number of stipe per bottle was 18.1, the size of pileus was 28 mm, and gill was crowded. The moisture content of 'Miso' was lower than that of Jangan No.5 and Wonhyeong No.1, and contents of protein, ash and sugar of 'Miso' were higher than those of other varieties, and tannin acid content of 'Miso' was lower than that of others. On the basis of AFLP analysis, the 'Miso' was distinct not only from Wonhyeong No.1, but also from their closest relative, oyster mushrooms.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.23 no.5
• /
• pp.767-773
• /
• 1994

The dietary fiber intakes of Korean were estimated based on the data from the National Nutrition Survey in 1989. From the data, 566 foods which contain dietary fiber were selected.The intake estimation was done based on these foods. Results were summarized as followed ; Mean DF intake of nationwide population was 17.53g/day and most of these were come from vegetables. Mean DF intake of rural population was slightly lower than that of urban's. Major sources of DF were vegetables, cereals & grain products, seaweeds, fruits, fungi & mushrooms and legumens & their products in order. Vegetables were the major sources of DF which occupied 32.4% of total intake. Among them, kimchi and red pepper were the representative sources. Among cereals & grain products, rice was found to be the most important DF source occupying 12.2% of total DF. And fried noodle, loaf bread and barley were followed. Sea mustard among seaweeds, apples and persimmons among fruits, oyster mushroom and mushroom among fungi & mushrooms and soybean sprout among legumes & their products were favorable sources of DF. 653 households out of 1, 925(33.9%) were consummed 10~15g of DF per day.

• The Korean Journal of Mycology
• /
• v.12 no.1
• /
• pp.1-8
• /
• 1984

The mycelial growth of some mushrooms was inhibited by benomyl treatment. The $ED_{50}$ of benomyl to that of Pleurotus spp., Agaricus bisporus and Flammulina velutipes was 25ppm, 50ppm and 200ppm, respectively, which indicates the former was the most sensitive to the fungicide. The mycelial growth of mushrooms growing on artificial media amended by benomyl was increased when they were cultured successively 5 times and 10 times on the media. The increasing rate of that of each mushroom was the highest at the concentration of $ED_{50}$ of benomyl. The mycelial growth of P. ostreatus was increased progressively as the number of successive culturing increased, while that of P. florida and A. bisporus was increased until they were cultured successively up to 5 times and 7 times, respectively, but they were decreased after that. Mutant sectors of mycelia were induced by successive treatment of benomyl. Mutant sectors of P. ostreatus appeared earlier than those of P. florida and all of them were induced earlier on the media of low contration of benomyl than on that of high concentration. The mycelia of mutant sectors induced by benomyl treatment grow faster than those of mother colony treated with benomyl successively, but there was no difference in resistance against the fungicide between them. The increase of mycelial growth of the mushrooms culturing successively on media containing benomyl indicated that they might obtain the resistance against benomyl.

• Journal of the East Asian Society of Dietary Life
• /
• v.23 no.5
• /
• pp.637-644
• /
• 2013

This study was conducted to obtain the baseline data for fermented tea with King oyster (Pleurotus eryngii) mushroom mycelium as well as to investigate the possibility of development for deodeok processing products. For this research, the physicochemical properties and antioxidant activity for fermented tea were confirmed by different roasting temperatures and roasting times. The results of the physicochemical properties, browning, turbidity, reducing sugar, total polyphenol and total flavonoid contents, were increased with increasing roasting temperature and roasting time, whereas the pH was decreased. The results of the antioxidant activity test of fermented tea, DPPH radical-scavenging activity, FRAP and reducing power, were increased with increasing roasting temperature and roasting time. The preference of fermented tea was confirmed as being the highest when roasted at $180^{\circ}C$ for 30 min. Although fermented tea did not have a unique flavor of mushroom and burnt flavor, it had strong color, flavor and taste. For this reason, fermented tea production for high content of functional materials and high preference is possible by roasting at $180^{\circ}C$ for 30 min. As for the results of this study, the physical properties of fermented deodeok were relatively stable on high temperature exposure and furthermore, the development of a variety of foods will be possible because fermented deodeok has an antioxidant capacity.

• Korean journal of applied entomology
• /
• v.38 no.3
• /
• pp.241-247
• /
• 1999

This study was aimed to elucidate the physiological function of a pair of protuberances like bifurcated horns developed on the anterior part of pupa in Coboldia fuscipes (Diptera: Scatopsidae), a pest on the oyster mushroom. In the morphological characteristics, larvae had a pair of 0.04 mm long microspines at their second body segment. Pupae had a pair of protuberances like bifurcated horns of 0.55 mm long on the anterior part and six pairs of spines, 0.12 mm long, at the posterior part of the abdomen. The microspines-removed 4th instar C. fuscipes larvae developed into pupae without a pair of the protuberances. After emergence, however, the adults have a pair of antenna normal regardless of with or without the protuberances. As ecological characteristics at the pupation, the pupa without a pair of the protuberances have a tendency to pupate outside the medium. However, development characteristics of the microspines-removed larvae were very similar to those of control larvae. In the result of microscopic observation, a pair of the protuberances was connected wit six pairs of spines by lateral longitudinal trunck and formed a tracheal system. In conclusion, this results implicates that the protuberances have an important role as a respiratory horn during the pupal period.

• Journal of Life Science
• /
• v.32 no.11
• /
• pp.890-898
• /
• 2022

This study was carried out to evaluate the effect of fermentation by Lactobacillus acidophilus on the antioxidant activity of Protaetia brevitarsis larvae fed with mushroom substrates (king oyster mushroom). The total polyphenol content of the P. brevitarsis larvae extracts (PLEs) (93.33±0.98 mg GAEs/extract g) was higher than that of the fermented P. brevitarsis larvae extracts (FPLEs) (65.02±1.32 mg GAEs/extract g). The flavonoid contents of the PLEs and FPLEs were 18.3±1.57 QEs mg/extract g and 17.69±0.95 QEs mg/extract g, respectively. The DPPH radical scavenging activity showed no significant difference between the PLEs and FPLEs at a concentration of 2-4 mg/ml. However, at a concentration of 8 mg/ml or more, the DPPH radical scavenging activity of the FPLEs was higher than that of the PLEs. The reducing power of the FPLEs was also higher than that of the PLEs, and more than twice as high at a concentration of 1.6 mg/ml or more. The ORAC value of the FPLEs (79.22±0.72 μM TEs/extract g) was higher than that of the PLEs (74.34±0.37 μMTEs/extract g). A WST-1 assay of the RAW 264.7 cells indicated that the PLEs and FPLEs showed no cytotoxicity.

• Journal of Mushroom
• /
• v.22 no.2
• /
• pp.67-72
• /
• 2024

In this study, we investigated the microbial community of oyster mushrooms at different growth stages at the species level. Gram-positive bacteria were predominant in the presterilized medium. On the other hand, Gram-negative bacteria were predominant in the culture-completed medium, post-harvest medium, and fruiting bodies. In addition, Pseudomonas tolaasii, which is known to cause disease in mushrooms, was confirmed in the cultured medium, post-harvest medium, and fruiting bodies, and it was determined that the mycelium culture stage was contaminated, and the reason why no disease occurred was Sphingobacterium psychroaquaticum. It was confirmed that this was because the growth of Pseudomonas tolaasii was suppressed by producing a component called tolacin. As a result of confirming the diversity of microorganisms, it was confirmed that the presterilization medium contains a variety of microorganisms compared to other growth stages, and the diversity decreases in the order of culture completion medium, fruiting body, and post-harvest medium. showed a trend. As a result of microbial similarity analysis, it was confirmed that the cultured medium and the post-harvest medium showed similar microbial communities, and in the case of fruiting bodies, there were some similarities but overall differences.

• The Korean Journal of Mycology
• /
• v.12 no.2
• /
• pp.53-58
• /
• 1984

This experiment was performed to select the most suitable species of oyster mush­room for cultivation during summer season in Korea. The selection was made based upon mycelial growth on the ricestraw substrates, fruiting under hot weather conditions during the summer season in Korea and potential high productivity using six cultures from South-east Asia. The newly selected strain of Pleurotus sajor-caju (ASI 2070) grew well on the rice straw substrates and produced the higher yields of sporophores than other cultures tested. The primordia of the mushroom were formed between 10 to $35^{\circ}C$. The optimal temperature for fruiting ranged from 20 to $25^{\circ}C$.