• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2002년도 추계국제학술대회
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• pp.164-164
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• 2002

에스트로젠을 처리한 송사리의 간으로부터 choriogenin vitellogenin estrogen receptor의 발현량을 전사수준에서 Real-time을 사용하여 정량.비교하였다. 시험어종으로는 부화 후 5개월 이상된 성숙한 수컷 송사리(Oryzias latipes)를(체중 약 250mg/마리)를 사용하여 17$\beta$-estradiol(25ppt, 50ppt, 100ppt)에 24시간 노출시켰다. Fluorescence dye는 choriogenin vitellogenin estrogen receptor의 경우 FAM (6-carboxyfluorescein)을 사용하였으며, $\beta$-actin의 경우는 VIC를 사용하였다. 프로브에 사용하는 quencher dye는 TAMRA(6-carboxy-N',N',N',N'-tetramethyl rhodamine)을 사용하였다. Internal control로 사용된 $\beta$-actin은 17$\beta$-estradiol의 농도에 상관 없이 0~10pM 범위에서 일정하게 발현됨을 보여주었다. vitellogenin choriogenin L 및 choriogenin H는 17$\beta$-estradiol의 농도에 의존하여 발현이 증가되는 용량-반응양상(Dose-dependent)을 나타내었다. 반면, estrogen receptor는 모든 처리군에서 $10^{-2}$pM 정도로 발혐됨에 따라 본 시험농도의 17$\beta$-estradiol에 의해서는 거의 유도발현이 되지 않음을 보여주었다. choriogenin L, choriogenin H, vitellogenin I 및 estrogen receptor 발현감수성을 비교한 결과, 25ppt 및 50ppt의 17$\beta$-esoadiol 농도에서는 ChgL > ChgH > VTG I >ER의 순으로 감수성이 높았으며, 100ppt 노출에서는 ChgL > VTG I > Chg H > ER의 순으로 감수성이 높게 나타났다. 결론적으로 choriogenin이 에스트로젠물질에 의한 가장 민감한 생체지표임을 알 수 있었다.

• 한국환경보건학회지
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• 제30권3호
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• pp.264-271
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• 2004

An aquatic food chain was constructed to provide information of bioaccumulation of DEHP as followed: phytoplankton(Scenedesmus subspicatus) ${\rightarrow}$ zooplankton(Daphnia magna) ${\rightarrow}$ fish(Oryzias latipes). After 10 days of exposure to DEHP, the fish and culture water were analyzed for residual concentration of DEHP and BAF(Bioaccumulation Factor) was determined. In addition, BCF(Bioconcentration Factor) was calculated in exposure tank in which fish were only exposed DEHP by culture water. These experiments provide the relative importance between BAF and BCF. In this study, BCF and BAF did not show any significant difference. Another work in this study was model construction and application to investigate the effect of food chain structure to BAF in higher organism (fish). The model constructed in this study considered the biological characteristics of DEHP such as metabolic parameters, as well as the chemical characteristics such as solubility. This model could be used in prediction of bioaccumulation level in dependent of various food chain structures, when the target organisms or chemicals would be changed.

• 한국응용과학기술학회지
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• 제14권3호
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• pp.29-38
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• 1997

Low toxic concentrated oil dispersant using n-Paraffin and Di(ethylene glycol)mono butylether mixed solvent was prepared, and tested by oil dispersant performance test method, and oil dispersant efficiency was measured using vertical shaking method to 3 kinds of Crude oil, Bunker oil and W/O emulsions with different physical properties by appling the prepared dispersant. Although toxicity test was performed with Flat fish and Rock fish by appling the mixed oils emulsified using prepared oil dispersant, couldn't find the toxicity to them. Concentrated oil dispersant prepared has a good dispersion efficiency of 97.2% after 0.5min settling time and 28.3% after 10min settling time to Bunker B oil with 10% water solution. Especially, the concentrated oil dispersant showing the low toxicity to Oryzias Latipes(24hr, TLm) was 54,000 ppm and to Brine Shrimp Artemia(24hr, TLm) was 51,000ppm, and also, it was completely biodegradated to 99.1% after $7{\sim}8$days.

• Mycobiology
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• 제47권2호
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• pp.135-142
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• 2019

Many members of the Saprolegniales (Oomycete) cause mycoses and disorders of fishes, of which Achlya and Saprolegnia are most ubiquitous genera worldwide. During a survey of the diversity of freshwater oomycetes in Korea, we collected seven isolates of Achlya, for which morphological and molecular phylogenetic analyses enabled them to identify as Achlya americana and Achlya bisexualis. In Korea, only a species of Achlya, A. prolifera, has been previously found to cause seedling rot on rice (Oryza sativa), but none of the two species have been reported yet. Importantly, A. bisexualis was isolated from a live fish, namely rice fish (Oryzias sinensis), as well as freshwater, and this is the first report of Achlya-causing mycoses on freshwater fishes in Korea. The presence of A. americana and A. bisexualis on live fish in Korea should be closely monitored, as considering the well-known broad infectivity of these species it has the potential to cause an important emerging disease on aquaculture industry.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권1호
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• pp.47-53
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• 2022

The escape behavior of prey fish to predator attack is directly linked to the survival of the fish. In this study, I explored the escape behavior of Medaka fish to bird attacks. To simulate the attack, I designed a model triangular-shaped bird to slide along a fishing line connected between rods at both ends of the tank. The triangular shape was set to 10×15 (S=1), 15×20 (S=2), and 20×25 cm (S=3) with base×height. The slope (θ) of the fishing line, which determines the attack speed of the model bird, was set to values of 15° (θ=1), 30° (θ=2), and 45° (θ=3). The escape behavior was characterized using five variables: escape speed (ν), escape acceleration (α), responsiveness (γ), branch length similarity entropy (ε), and alignment (ϕ). The experimental results showed when (S, θ)=(fixed, varied), the change in values of the five variables were not significant. Thus, the fish respond more sensitively to S than to θ In contrast, when (S, θ)=(varied, fixed), ν, α, and γ showed increasing trends but ε and ϕ did not change much. This indicates the nature of fish escape behavior irrespective of the threat is inherent in ε and ϕ. I found that fish escape behavior can be divided into two types for the five physical quantities. In particular, the analysis showed that the type was mainly determined by the size of the model bird.

• 한국환경복원기술학회지
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• 제13권4호
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• pp.42-51
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• 2010

The purpose of this study is to analyze the characteristics of environment-friendliness of Root Barrier materials based on comprehensive experiments on harmfulness of Root Barrier materials and fish toxicity of Oryzias latipes mortality to verify eco-toxicity of each method of construction and Root Barrier material, which are to be applied by taking eco-toxicity into account when building ecological flows in upper areas on natural and artificial grounds. As a result, the following conclusions have been reached in this study: 1. In regard of the harmfulness analyzed, each material showed a different result of analytical value in each lab tank. Compared to lab tank, pH (la), DO (lb), T-N (VI) and T-coli (la) were in the same grade, but COD, SS, T-P and F-coli were less than that of control, respectively increased or decreased by material were analyzed. 2. In the experiment of fish toxicity, Barrier sheet was found to have 66.7% of fish mortality, indicating strong fish toxicity. Synthetic rubber system root barrier sheet (20.0%) was analyzed to have medium fish toxicity, while Synthetic resin system root barrier-waterproof sheet (3.3%), Synthetic rubber system membrane root barrier sheet (3.3%) and Synthetic resin system root barrier sheet (0.0%) showed relatively lower mortality and fish toxicity. To sum up such results as found in the experiment mentioned so far, the values of harmfulness and root penetration resistance analyzed were different in each lab tank, but there was absolutely little correlation with the mortality gained from the experiment on fish toxicity. In the experiment of fish toxicity, environment-friendly root barrier materials were analyzed, and it was found that Synthetic resin system root barrier sheet, Synthetic resin system root barrier waterproof sheet and Synthetic rubber system membrane root barrier sheet are highly environment-friendly. In contrast, Synthetic rubber system root barrier sheet was found to have medium-level environment-friendliness. Also, Barrier sheet was analyzed to have low environment-friendliness.

• Environmental Analysis Health and Toxicology
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• 제18권2호
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• pp.145-153
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• 2003

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper of appropriate sublethal concentrations after starvation for 48 hr. The untreated individuals showed common behavioral characteristics (i.e. , smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000 ppb, activity decreated, and the fish showed an erratic movement. Fish were exposed to copper at various concentrations (0,100 and 1,000 ppb) for 24 hrs, and acetylcholine esterase (AChE) activity was observed. When fish were exposed to 1,000 ppb of copper, the body AChE activities appeared to decrease but the head AChE activities showed little change. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head (brain) and body (kidney) portions affected by the copper treatment were analyzed using immunohistochemical technique compared with control. Five organs of the fish (olfactory bulb, hyothalamus, optic lobe, pons and myelencephalon regions) showed a relatively strong TH protein expression in the control experiment. A differential expression of TH, however, was observed in the treatment (100 ppb and 1,000 ppb). The treatment (1,000 ppb) significantly suppressed TH protein production in the brain regions. In kidney, however, the same treatment caused little suppression compared with the control. Copper appeared to be less effective in suppression of TH than diazinon, a known TH suppressor. It was concluded that TH could be used at a potential biomarker to monitor the acute copper toxicity in Japanese medaka.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2005년도 국제학술대회
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• pp.345-350
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• 2005

Acute toxicities of five pharmaceutical products were evaluated with aquatic microbes, invertebrates, and fish. The test pharmaceuticals, i.e., cimetidine, carbamazepine, diltiazem, acetaminophene, and metformin have been often detected in aquatic environment, but theire cological hazard on receptors of various trophic levels has seldom been evaluated. In the present study, we conducted acute toxicity assays with a marine bacterium, Vibrio fischeri, an invertebrate, Daphnia magna, and a fish, Japanese medake (Oryzias latipes). In general, D. magna, showed the most sensitive response to the test chemicals. Diltiazem exhibited the lowest EC50 value after 96 hr of exposure at 7.6 mg/L, followed by cimetidine >acetaminophen > metformin = carbamazepine in an order of decreasing susceptibility. With the fish, diltiazem and carbamazepine showed the 96 hr EC50 values at 14.1${\sim}$35.4 mg/L while acetaminophen, cimetidine, and metformin did not cause 50% mortality at 100 mg/L. Similar pattern was noted with the Microtox Assay, with which the median effective concentrations for acetaminophen, cimetidine, and metformin were found at the range between 301.8 and 755.4 mg/L. Carbamazepine and diltiazem exposure to the microbes resulted in EC50 values around 50 mg/L. Predicted no effect concentrations (PECs) of these pharmaceuticals derived from the EC5O values obtained from this study, and predicted environmental concentrations (PECs) obtained from available literatures were utilized to estimate ecological risks of the test compounds. No test pharmaceuticals resulted in risk quotients (PEC/PNEC) greater than 1, which suggests no serious potential ecological concerns. It should be noted however that further studies including the refinement of PEC derivation, identification and toxicity assessment of the metabolites and/or their interactions with other stressors may be warranted to better understand the environmental consequences of the residual pharmaceutical discharge to the waterway.

• Environmental Analysis Health and Toxicology
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• 제22권2호통권57호
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• pp.171-175
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• 2007

Indigenous species means a species that is likely, due to historical presence, to occur at a specified site for some portion of its life span. Therefore, indigenous species can be useful as an indicator to assess environmental risk caused by hazardous chemicals in a specific site. So far a few toxicity studies using freshwater species which are indigenous to Korea have been carried out. In this study, a freshwater shrimp (Neocardina denticulata) indigenous to Korea was used for acute toxicity test of heavy metals. Neocardina denticulata were exposed to cadmium chloride $(CdCl_2)$, copper chloride $(CuCl_2)$ and zinc chloride $(ZnCl_2)$ using automatic flow-through system for 96 hours. The 96h LC50s were calculated as 0.043 $(0.042{\sim}0.045)mg\;CdCl_2/L,\;0.104(0.098{\sim}0.113)mg\; CuCl_2/L\;and\;2.021\;(1.633{\sim}2.594)mg\;ZnCl_2/L$. When compaired with some international standard species such as medaka(Oryzias latipes), Neocardina denticulata had high sensitivity. Therefore, this study suggested that Neocardina denticulata have possibilities for a sensitive test species to test heavy metal toxicity in aqua-system.

• 생태와환경
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• 제38권3호통권113호
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• pp.420-428
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• 2005

유해조류의 대발생을 제어하기 위하여 개발되었던 박테리아, 백운몰개, 송사리 등의 직접 및 배양여액이 배양중인 단독형 (solitary) 남조 M. aeruginosa의 성장 및 형태에 어떠한 영향을 주는지 파악하기 위하여 대수기 조류세포에 생물제재를 농도별로 처리하였다. 실험에 사용된 3가지 생물제재의 적접처리시 남조 M. aeruginosa를 효과적으로 감소시켰으며, 특히 2종의 어류는 모두 처리 5일째 조류세포를 완전히 제어하였다. 생물제재의 직접 또는 여액에 의한 조류세포의 군체형성은 생물제재의 종류, 처리유형, 처리농도 등에 따라 차이를 보였는데, 어류의 경우, 여액 처리농도가 높을수록 높은 군체 및 군체당 높은 세포수를 나타냈으나, 살조세균은 농도에 따른 유의한 차이를 보이지 않았다. 결국, 유해조류의 제어를 위한 생물제재의 현장 적용은 남조 M. aeruginosa의 군체형성을 유도함으로서 새로운 조류대발생의 원인이 될 수 있기 때문에 새로운 생물제재나 적용기법 등의 개발이 필요하다고 판단되었다.