• 한국유기농업학회지
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• 제9권1호
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• pp.37-49
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• 2001

This experiment was carried out to investigate the effects of application levels of nightsoil sludge compost(NSC) in 3 and 4 cuttings per annum on the dry matter yields and nutritive value of orchardgrass(Dactylis glomerata L.). Annual nightsoil sludge compost(NSC) were applied at levels of 0, 10, 20, 40 and 80 ton $㏊^{respectively}$ . Relative yields of each cut to annual dry matter yield were 29.9%, 46.% and 23.2% for 1st cut, 2nd cut and 3rd cut in 3 cutting system, 23.7%, 29.3%, 34.0% and 13.0% for 1st cut, 2nd cut, 3rd cut and 4th cut in 4 cutting system, respectively. Significantly higher dry matter yield of orchardgrass obtained were 8.11 ton $㏊^{-1}$ at level of 40 ton ㏊$^{-1}$ $yr^{-1}$ in 3 cutting systems, ranges of 7.50~10.71 ton $㏊^{-1}$ at level of 10~80 ton ㏊$^{-1}$ $yr^{-1}$ in 4 cutting systems than those of non application level of NSC, respectively(p〈0.05). The mean efficiency of dry matter production to application of NSC(kg DM/ton NSC) were 46 and 102 kg in 3 and 4 cutting systems, respectively. Although th contents of crude protein(CP, %) of orchardgrass were significantly higher at the application levels of 10~80 ton $㏊^{-1}$ $yr^{-1}$ -1/ by NSC than those of the application levels at the 0 ton $㏊^{-1}$ $yr^{-1}$ in 3 and 4 cutting systems(p〈0.05), the contents of neutral detergent fiber(NDF, %) and acid detergent fiber(ADF, %) were lower in NSC plots than those at the non-application plots of NSC. The contents of total digestible nutrient(TDN, %) and relative feed value(RFV) of orchardgrass were significantly increased with increase the applicaton levels of NSC(p〈0.05).

• 한국초지조사료학회지
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• 제20권4호
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• pp.259-264
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• 2000

오차드그래스의 완숙 종자배양에서 배지 내에 첨가되는 copper의 농도가 캘러스 형성과 식물체 재분화율에 미치는 영향을 조사하였다. 캘러스의 유도는 $2\;mg/{\ell}$의 2,4-D와 $0.1-100\;{\mu}mol$의 copper sulfate를 첨가한 MS 배지를 이용하였으며, 식물체 재분화는 $1\;mg/{\ell}$의 NAA, $5\;mg/{\ell}$의 kinetin 및 $0.1-100\;{\mu}mol$의 copper sulfate가 첨가된 N6 배지를 이용하였다.그 결과, copper의 첨가는 캘러스 형성능력에는 유의성있는 차이를 나타내지 않았으나, copper가 첨가된 배지에서 생성된 캘러스에서 백색이면서 조직이 치밀한 즉, 식물체 재분화에 적합한 캘러스의 비율이 높게 나타났다. 식물체 재분화율은 copper가 첨가된 배지에서 생성된 캘러스가 첨가되지 않은 배지에서 생성된 캘러스보다 높게 나타났으며, 특히 $70\;{\mu}mol$의 copper가 첨가된 백지에서 생성된 캘러스가 대구조에 비해 2.5배 이상 증가된 가장 높은 재분화 능력을 나타내었다. 또한, 캘러스당 재분화된 식물체의 수는 $70\;{\mu}mol$의 copper가 첨가된 배지에서 생성된 캘러스에서 대조구에 비해 2.6배 이상 증가된 것으로 나타났다.

• 한국초지조사료학회지
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• 제14권3호
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• pp.215-222
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glorneruta L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: 1. Herbage utilization of mixture with Hallmark variety(T2) was the highest as 78.2% and that with Rancho variety(T5) was the lowest as 70.2%. but there was no difference(P>0.05). Mixtures with early maturing varieties(T1, T2 and T3) were higher as 77.4% than those with late varieties(T4, T5 and T6) as 72.9% in herbage utilization. In monocultures Rancho variety(T5) was the highest as 68.6% and Lidacta variety(T4) was the lowest as 58.8%, and mixtures with early maturing varieties(T1, T2 and T3) as 66.6% were higher than those of late(T1, 72 and 73) as 64.6%. 2. The content of crude protein of 6 mixed pastures ranged from 19.7% to 22.8%, and those of ether extracts, crude fiber, crude ash and nitrogen free extract were 6.6 to 7.5%, 24.9 to 26.2'76, 1 1.5 to 1 1.9% and 33.6-35.4 8, respectively, But there were not different significantly(P>0.05). In monocultures, significant difference by variety was observed in crude protein content(P<0.05). 3. In botanical composition, there was just a little different among 6 mixtures, having 90% or more in grass percentage. The percentages of each orchardgrass varieties were decreased, but those of perennial ryegrasses were increased as grazing sesson was progressed in all treatment.

• 한국초지조사료학회지
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• 제14권3호
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• pp.206-214
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glomrrata L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: I. Heading date of early maturing varieties among orchardgrass like as Potomac, Hallmark and Syn. 2 ranged from May 9 to May 14. Otherwise those of late varieties(Lidacta Rancho and Syn. 10) were between May 21 to May 25. Leaf types of early varieties were shown erect, those of late varieties were erect or semi-erect. 2. In dry matter production per ha, mixture with Rancho variety(T5) was the highest as 7.818 kg and mixture with Hallmark(T2) was the lowest as 7,013 kg. But there was not significantly different(P>0.05) among mixtures. While in monocultures, Syn. 10 variety was the highest(6.626 kglha) and Lidacta was Iowest(5,775 kg). But no difference was found(P>0.05). 3. In mean grass height after grazing, mixtures with early maturing types were higher as 9.8 cm than that of late (8.8cm), and early types were higher as 10.5cm than that of late(9.4cm) in monocultures. But there was no significant difference both mixtures and monocultures.

• 한국초지조사료학회지
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• 제10권1호
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• pp.1-9
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• 1990

Tillering of orchardgrass during establishment year was considered as an important factor for ground cover and forage yield. Field experiment was conducted to examine seasonal fluctuation of tiller number in three cultivars of orchardgrass; Potomac, Hall-mark, and Sumas, after transplanting with five planting density. Also, effects of plant density and nitrogen application in cultivar, Potomac were studied on tillering, forage yield and various growth characteristics in pot experiment Tillers per plant decreased with increased density and showed seasonal variations. It increased up to July, decreased during summer months, and increased again from October. Sumas had less tillers than other cultivars, especially at high density. Tillers per unit area kept high during spring. After then, there was stady decrease through summer and remained constant without affecting by planting density. Nitrogen application increased tillers per unit area in Potomac up to 20kg/10a, but decreased its tiller production at 40kg/lOa. Maximum number of tiller was obtained in the combinations of 20kg application of nitrogen with higher density than 324 plant per square meter. Forage yield increased as nitrogen application increased, while it did not respond to plant density. Forage yield had high linear correlation between LAI, leaf number with a peak at 2800 culrns. For high forage yielding, it was suggested to obtain optimum number of tillers per unit area in early season as possible, applying of adequate amount of nitrogen with relative high density depend on cultural condition.

• 한국초지조사료학회지
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• 제8권3호
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• pp.104-109
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• 1988

A field experiment was conducted in order to investigate the seasonal changes of leaf growth and related characteristics in three cultivars of orchardgrass; Potomac, Kay and Sumas. The results were summarized as follows: 1. Leaf elongation was increased in a nearly linear phase during first and third cutting stages. It was increased slowly in early 10 days to 15 days after cutting and increased rapidly thereafter during the rest cutting stages. In cultivars, Potomac was showed hlgher leaf elongation than other cultivars during all cutting stages. There was no difference of leaf width within cutting stages, but the leaf width of fall regrowth was narrow. Sumas had relatively short and wide leaves. 2. Leaf dry weight and leaf area in first cutting stage were larger than others. Leaf area was increased rapidly from 15 days after cutting and leaf $we$ was increased rapidly from 20 days over all cutting stages. The increase in leaf area and dry weight were slow down after 30 days. 3. Number of epidermal cells was increased rapidly after cutting and the rate of increase was slow down after 30 days. In a cross section of leaf tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissues. Leaf tissue had a large vacancy at early growth period after harvest and was filled gradually with mesophyll. This result was related to the increase of leaf dry matter.

• 한국초지조사료학회지
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• 제7권2호
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• pp.71-78
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• 1987

본 실험은 파종방법, 배수화도 및 여름철 예취관리가 오차드그라스 채초지의 목장건물수량, 고사물량, 초지의 잡초발생 및 피복율에 미치는 영향을 조사하기 위하여 심험설계는 주구를 조파와 산파로 하고 세구를 배수랑호구와 배수불랑구로 하였으며, 세세구는 장마전 예취구와 장마후 예취구를 두어 세세구 배치 3반후으로 하여 1983년 9월부터 1984년 10월까지 실시하였던바 얻어진 결과는 다음과 같다. 1. 초지의 목장건물수량은 1회 예취시에는 처리간에 차이가 없었으나 2회와 3회 예취시에는 장마전 예취구가 장마후 예취구에 비하여 수량이 높았고 ($P{\le}0.01$) 3회예취시에는 조파구가 산파구에 비하여 수량이 높았다. $P{\le}0.05$. 2. 목장의 고사물량은 2회예취시에만 발생하였는데 장마전 예취구에서는 발생하지 않았는데 반해 장마후 예취구에서는 배수랑호구와 배수불랑구에서 총 목장수량에 대하여 각각 24%와 35%의 고사물량이 발생하였다. 3. 잡초발생은 1회, 2회예취시에는 거의 없었으나 3회 예취시부터 다량발생하였는데 산파구가 조파구에 비하여 높았고 ($P{\le}0.05$) 장마후 예취구가 장마전 예취구에 비하여 월등이 높았다. $P{\le}0.01$. 4. 목장의 피복율은 1회 예취율에는 거의 100%로 차이가 없었으나 2회 및 3회 예취후에는 장마전 예취구가 장마후 예취구에 비하여 매우 높은 수준을 보였으며 ($P{\le}0.01$) 조파는 산파에 비하여 높았으나 유의차는 없었다. 또한 각 처리구의 월동전식생 피복율은 장마전 예취구는 장마후 예취구에 비해 오차드그라스의 구성비율은 높았고 잡초 및 나지의 구성비율은 낮았다. 5. 본 실험결과로 미루어보아 오차드그라스채초지의 2회 예취는 장마전에 실시하고 조파를 함으로서 잡초발생을 크게 줄일 수 있을 것으로 생각된다.

• 한국초지조사료학회지
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• 제25권4호
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• pp.267-274
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• 2005

고온 내성 오차드그라스를 개발하기 위하여, 재조합 DgP23 유전자에 CaMV 35S 프로모터를 붙여 발현벡터를 제작, Agrobacterium 형질전환 방법으로 오차드그라스 형질전환체를 생산하였다. Genomic DNA를 분리하여 PCR 및 Southern blot 분석을 실시한 결과, PCR 분석에서 DgP23 유전자의 DNA band가 관찰되었고, Southern blot 분석에서도 X-ray film 상에 hybridization signal이 관찰되어, 오차드그라스 genome에 DgP23 유전자의 도입이 확인되었으며, wild type 및 empty vector control에서는 DNA band 및 hybridization signal이 관찰되지 않았다. 또한 RT-PCR 및 이들 산물의 Southern blot 분석 결과, DgP23 유전자의 정상적인 발현이 확인되었다. 형질전환 오차드그라스를 온실 및 포장에서 재배하며 생육특성을 조사한 결과, 비형질전환체와 비교하여 형태적 차이는 나타내지 않았다. 실험실 조건에서 고온내성을 조사한 결과, 고온내성이 확인되지 않았기 때문에 형질전환 종자를 생산하여 포장조건에서 고온내성을 검정할 계획이며, 재배시험에서는 내성이 강한 개체를 선발할 수 있을 것으로 예상된다.

• 한국초지조사료학회지
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• 제11권3호
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• pp.169-174
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• 1991

For simple estimation of pasture production and nutritive value in relation to height of orchardgrass (Ductylis glomumtu L.), the experiment was carried out in a pasture mixture dominated by orchardgrass. The samples of grasses were harvested at the heights of about 30, 40, 50, 70 and 90 cm at the first harvest under different percentage of 100 (grass coverage): 0 (bareland), 80 : 20, 60 : 40, 40 : 60 and 20 : 80, respectively. As the height of orchardgrass was increased, fresh and dry matter (DM) yield were significantly increased. The regression equation between DM yield (Y) and height (X) was Y=ll. 5390 X- 146.60 (r=O. 9414 * * ). Also the higher bareland, the lower pasture production was observed. As the plant height at the first harvest was increased, the contents of crude (C) protein, C. fat, C. ash and relative feed value were markedly decreased. On the other hand, the contents of C. fiber, NFE, NDF. ADF, cellulose and lignin were significantly increased, respectively. The higher height, the lower DM digestibility was found. The equation between digestibility (Y) and height (X) was Y=- 0.2193 X+ 80.99 (r = - 0.9862**). Considering DM yield, digestibility and crude protein, the optimum height of orchardgrass at the first harvest was 59 to 67crn for better pasture production and nutritive value.

• 한국유기농업학회지
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• 제3권1호
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• pp.71-78
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• 1994

This experiment was conducted to investigate the effects of mixed application of earthworm cast and N-fertilizer on dry matter(DM) yield in orchardgrass(Dactylis glomerate L.). Experimental plot were designed by a randomized block design with 3 replications of six different treatments-Control(0:0), 100% earthworm cast(0:100), 3 levels of mixed application(25:75:, 50:50, 75:25) of earthworm cast and N-fertilizer, and 100% N-fertilizer(100:0)based on content (%) of nitrogen. The experiment was carried out from March to October, 1993, on the forage experimental field of Kon-Kuk Univ. in Seoul. The results obtained are summarized as follows: 1. Annual dry matter yield was significantly increased according to increasing of N-fertilizer 2. Dry matter yield for each treatment as cutting time was passing was decreased. However, dry matter yield was slightly increased at 75%, 50%, and 25% mixed application of earthworm cast at the third cutting time than the second cutting time(p<0.05). 3. concerning each treatment per cutting time, dry matter yield was higher at 100% N fertilizer than the other treatments at the first, second, and fourth cutting time except the third cutting time only(p<0.05). And according to decreasing of mixing ratio of N fertilizer, dry matter yield was decreased, too. 4. Dry matter yield of 100% earthworm cast was significantly higher than control at the fourth cutting time(p<0.05).