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http://dx.doi.org/10.5333/KGFS.2005.25.4.267

Production of Transgenic Orchardgrass Overexpressing a Thermotolerant Gene, DgP23  

Kim Ki-Yong (National Livestock Research Institute)
Jang Yo-Soon (Korea Ocean Research & Development Institute)
Park Geun Je (National Livestock Research Institute)
Choi Gi Jun (National Livestock Research Institute)
Seong Byung Ryul (National Livestock Research Institute)
Seo Sung (National Livestock Research Institute)
Cha Joon-Yung (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Son Daeyong (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.25, no.4, 2005 , pp. 267-274 More about this Journal
Abstract
To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, a thermptolerance gene, DgP23, was introduced into orchardgrass using Agrobacterium - mediated transformation method. PCR and Southern blot analyses using genomic DNA showed specific DNA band on agarose gel and hybridization signal on X- ray film in transgenic orchardgrass harboring the recombinant DgP23 gene, but not in the wild type and empty vector control plants. RT-PCR and Southern blot analyses using total RNA also showed specific DNA band and hybridization signal. Transgenic orchardgrass did not showed ny morphological aberration both in the green house and field cultivation. Thermotolerance of transgenic plants was not detected in laboratory test. but may detected in field test.
Keywords
Agrobacterium - mediated transformation; DgP23 gene; Orchardgrass (Dactylis glomerata L.); Thermo-tolerance;
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1 Kim, K.Y., Y.W. Rim, K.J. Choi, J.S. Shin, J.G. Kim and J. Jo. 1998. Rapid regeneration of plants on N6 medium from orchardgrass (Dactylis glomerata L.) calli. J. Korean Grassl. Sci. 18:267-272
2 Kim, K.Y., Y.W. Rim, K.J. Choi and B.R. Ryul Sung. 1999a. Callus induction from seeds of birds foot trefoil and plant regeneration on BOi2Y medium. J. Korean Grassl. Sci. 19:303-308
3 Kim, K.Y., Y.W. Rim, K.J. Choi, Y.S. Jang, W.H. Kim, S. Seo, B.H. Lee and J. Jo. 1999b. Callus formation from alfalfa (Medicago sativa L.) seed and plant regeneration from alfalfa calli. J. Korean Grassl. Sci. 19:23-30
4 Mollerup, J. and M.W. Berchtold. 2005. The co-chaperone p23 is degraded by caspases and the proteasome during apoptosis. FEBS Letters 579:4187-4192   DOI   ScienceOn
5 Lagrimini, L.M., R.J. Joly, J.R. Dunlap and T.T. Liu. 1997. The consequence of peroxidase overexpression in transgenic plants on root growth and development. Plant Mol. Biol. 33:887-895   DOI   ScienceOn
6 Murray, M.G. and W.F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4325   DOI   ScienceOn
7 Bae, E.K., I.A. Lee, K.Y. Kim, B.H. Lee, D.Y. Son, H.S. Lee, M.S. Chung and J. Jo. 2002. Comparison of callus formation ratios from seed explants, callus sizes and regeneration efficiency among several Orchardgrass (Dactylis glomerata L.) varieties. J. Korean Grassl. Sci. 22:93-100   DOI   ScienceOn
8 Choi, G.J., Y.W. Rim, K.Y. Kim, S.H. Choi, B.R. Sung, W.H. Kim, D.E. Shin and Y.C. Lim. 2000. A cold-tolerant and high-yielding italian ryegrass (Lolium multiflorum L.) new variety 'Hwasan 101'. J. Korean Grassl. Sci. 20:1-6
9 Choi, G.J., Y.W. Rim, Y.C. Lim, K.Y. Kim, B.R. Sung, S.H. Choi and G.J. Park. 2001b. Growth characters and productivity of new italian ryegrass (Lolium multiflorum L.) variety 'Hwasan 103'. J. Korean Grassl. Sci. 21:163-168
10 McGookin, R. 1984. RNA extraction by the guanidine thiocyanate procedure. In: Walker J.M. (ed), Methods in Molecular Biology. Vol. 2, Humana Press, New Jersey, pp. 113-116
11 Wochnik, G.M., J.C. Young, U. Schmidt, F. Holsboer, F.U. Hartl and T. Rein. 2004. Inhibition of GR-mediated transcription by p23 requires interaction with Hsp90. FEBS Letts 560:35-38   DOI   ScienceOn
12 Zhu, S. and J. Tytgat. 2004. Evolutionary epitopes of Hsp90 and p23: implications for their interaction. FASEB J. 18:940-947   DOI   ScienceOn
13 Rim, Y.W., G.J. Choi, B.R. Sung, Y.C. Lim, M.J. Kim, G.J. Park, K.Y. Kim, J.W. Chung and N.G. Park. 2003. Growth characterizatics and productivity of new orchardgrass (Dactylis glomerata L.) variety 'Jangbeol 101'. J. Korean Grassl. Sci. 23:207-210   DOI   ScienceOn
14 Rim, Y.W., G.J. Choi, B.R. Sung, Y.C. Lim, M.J. Kim, G.J. Park, K.Y. Kim, J.W. Chung and S.B. Go. 2004. Growth characteristics and productivity of new orchardgrass (Dactylis glomerata L.) J. Korean Grassl. Sci. 24:261-264   DOI   ScienceOn
15 Shirafuji, H., X. Xuan, I. Kimata, Y. Takashima, S. Fukumoto, H. Otsuka, H. Nagasawa and H. Suzuki. 2005. Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects. J. Parasitol 91 :476-479   DOI   ScienceOn
16 Son, D., K.Y. Kim, Y.S. Jang, H. Lee, S.H. Won, B.H. Lee, M.H. Kim and J. Jo. 2001. Root initiation in cut alfalfa stems by treatment of IBA. J. Korean Grassl. Sci. 21 :27-30   과학기술학회마을
17 Lee, K.W., S.H. Lee, D.G. Lee, H.S. Woo, D.H. Kim, M.S. Choi, K.Y. Kim, H. Lee and B.H. Lee. 2005. Effect of plant growth regulators and antitoxidants on callus induction and plant regeneration from seed culture of orchardgrass. J. Korean Grassl. Sci. 25:191-198   DOI   ScienceOn
18 Lee, B.H., S.H. Won, H. Lee, K.Y. Kim and J. Jo. 2000b. Efficient Agrobacterium-mediated transformation of alfalfa using secondary somatic embryogenic callus. J. Korean Grassl. Sci. 20: 13-18   과학기술학회마을
19 Lagrimini, L.M. 1991. Wound-induced deposition of polyphenols in transgenic plants overexpressing peroxidase. Plant Physiol. 96:577-583   DOI   ScienceOn
20 Lee, H., Y. Kwon, B.H. Lee, S.H. Won, K.Y Kim and J. Jo. 2000a. Plant regeneration from embryogenic suspension culture of orchardgrass (Dactylis glomerata L.). J. Korean Grassl. Sci. 20:7-12
21 Lee, H., E. Bae, K.Y. Kim, S. Won, M. Chung and J. Jo. 2001. Transformation of Orchardgrass (Dactylis glomerata L.) with Glutathione Reductase Gene. J. Korean Grassl. Sci. 21:21-26
22 Kim, K.Y., K.M. Kang, E.K. Bae, I.A. Lee, Y.W. Rim, G.J. Choi, G.J. Park, D. Son and J. Jo. 2003a. Callus formation ratio and regeneration efficiency of orchardgrass varieties developed in Korea. J. Korean Grassl. Sci. 23:59-64   과학기술학회마을   DOI   ScienceOn
23 Kim, K.Y., B.R. Sung, Y.W. Rim, G.J. Choi, Y.C. Lim, Y.S. Jang, E.S. Chung, W.H. Kim and J.G. Kim. 2001a. Transformation of birdsfoot trefoil by BcHSP 17.6 gene using Agrobacterium tumefaciens. J. Korean Grassl. Sci. 21:145-150
24 Kim, K.Y., B.R Sung, Y.W. Rim, G.J. Choi, Y.C. Lim, Y.S. Jang, S. Seo, S.H. Yoon, G.J. Park and J. Jo. 2001b. Transformation of alfalfa by BcHSP17.6 gene using Agrobacterium tumefaciens. J. Korean Grassl. Sci. 21:151-156
25 Kim, K.Y., G.J. Choi, B.R. Sung, Y.W. Rim, Y.C, Lim, Y.S. Jang and W.H. Kim. 2001c. Root initiation in cut birds foot trefoil stems by treatment of IBA. J. Korean Grassl. Sci. 21:35-38
26 Kim, K.Y, K.M. Kang, G.J. Park, E.K. Bae, I.A. Lee, B.H. Lee, S.S. Kwak and J. Jo. 2003b. Examination of root induction ratio for regeneration of alfalfa by medium component. J. Korean Grassl. Sci. 23:95-100   과학기술학회마을   DOI   ScienceOn
27 Kim, K.Y., K.M. Kang, B.R. Sung, M.J. Kim, Y.W. Rim, W.H. Kim, G.J. Park and B.H. Lee. 2004a. Determination of heat killing temperature of alfalfa (Medicago saliva L.). J. Korean Grassl. Sci. 24:21-24   DOI   ScienceOn
28 Choi, G.J., Y.W. Rim, Y.C. Lim, K.Y. Kim, B.R. Sung, M.J. Kim, G.J. Park and S.R. Kim. 2001a. Growth characters and productivity of new italian ryegrass (Lolium multiflorum L.) variety 'Hwasan 102'. J. Korean Grassl. Sci. 21:157-162
29 Kim, K.Y, K.M. Kang, Y.W. Rim, G.J. Park, Y.C. Lim, S. Seo, D. Son and J. Jo. 2004b. Determination of heat killing temperature of orchardgrass (Dactylis glomerata L.). J. Korean Grassl. Sci. 24:25-28   DOI   ScienceOn
30 Kim, K.Y, Y.J. Choi, Y.W. Rim, B.R. Sung, S.J. Lee, J.S. Yang, R. Hahn, J.R. Kim and B.H. Lee. 2004c. Determination of heat killing temperature of birdsfoot trefoil and italian ryegrass. J. Korean Grassl. Sci. 24:341-346   DOI   ScienceOn
31 Gausdal, G., B.T. Gjertsen, K.E. Fladmark, H. Demol, J. Vandekerckhove and S.-O. Doskeland, 2004. Caspase-dependent, geldanamycin-enhanced cleavage of co-chaperone p23 in leukemic apoptosis. Leukemia. 18:1989-1996   DOI   ScienceOn
32 Gold, B.G. and Y.P. Zhong. 2004. FK506 requires stimulation of the extracellular signal-regulated kinase 1/2 and the steroid receptor chaperone protein p23 for neurite elongation. Neurosignals. 13:122-129   DOI   ScienceOn
33 Harst, A., H. Lin and W.M. Obermann. 2005. Ahal competes with Hop, p50 and p23 for binding to the molecular chaperone Hsp90 and contributes to kinase and hormone receptor activation. Biochem. J. 387:789-796   DOI   ScienceOn
34 Holsters, M., O.D. Wael, A. Depicker, E. Messens, M.V. Montagu and J. Schell. 1978. Transfection and transformation of A.tumefaciens. Mol. Gel. Genet. 163:181-187   DOI   ScienceOn