• 한국균학회지
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• 제19권2호
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• pp.136-142
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• 1991

수용성 치자(水溶性 梔子)(Ga. jasminoides의 열매)색소(色素)(등황색)(橙黃色)를 냉수(冷水)로 추출하여 함유시킨 yeast morphology agar(Difco)에 각종 효모균류(酵母菌類)를 배양하여 그들의 균집락(菌集落) 색깔변화를 관찰하여 다음과 같은 결과를 얻었다. 치자배지(梔子培地)에서 증식(增殖)한 Ca. guilliermondii 와 Ca. lusitaniae 의 균집락(菌集落)은 $28^{\circ}C$에서 3일이내에 군청색(群靑色)을 띠었고, Ca. tropicalis와 Ca. viswanathii 등은 옅은 회색(灰色)을 띠었는데 Ca. viswanathii는 7일후에 군청색(群靑色)으로 변했다. Ca. krusei와 To. glabrata 등은 $1{\sim}2$주후 회색(灰色)내지 옅은 회청색(灰靑色)을 띠었고 Sa. cerevi-siae는 연두색을 띠었다. 그러나 Ca. albicans와 Ca. parapsilosis 등은 색깔변화가 없었고, 적색(赤色) 균집락(菌集落)의 Rhodotorula sp.도 변화가 없었다. 그러나 Cr. neofor-mans는 갈색(褐色) 내지 자갈색(紫褐色) 균집락(菌集落)을 형성해 다른 모든 균종(菌種)과 뚜렷하게 구별되어 기존(旣存) 선별동정배지(選別同定培地)인 caffeic acid 또는 dopamine배지(培地)를 대체할 수 있을 뿐 아니라 몇몇 Candida spp.의 특정적 색깔을 관찰할 수 있어서 치자배지(梔子培地)가 임상검체(臨床檢體)로부터 효모균종(酵母菌種)의 분리배양(分離培養)에 매우 유용하다고 생각한다.

• 디지털융복합연구
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• 제14권11호
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• pp.449-457
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• 2016

본 연구에서는 과일즙이 복합레진의 표면부식과 착색에 미치는 영향을 알아보고자 일상적으로 많이 섭취하는 과일 중 산도가 높고 색깔이 있는 포도, 레몬, 오렌지, 자몽, 키위 5종을 선정하고 레진시편을 제작하여 과일즙에 침지하였다. 레진시편은 $37^{\circ}C$에서 과일즙에 3일, 7일 간격으로 침지하였다. 대조군은 과일즙에 침지하지 않은 레진으로 하였다. 시간에 따른 표면의 변화는 주사전자현미경을 이용하여 관찰하였으며 착색정도의 변화는 분광광도계를 이용하여 측정하였다. 주사전자현미경으로 레진의 표면변화를 관찰한 결과, 대조군에 비해 실험군은 표면 입자간의 홈의 크기가 증가하였고 직사각형모양의 막대상의 구조가 생겨났으며 작게 돌출된 돌기상의 입자가 사라지는 양상을 보였다. 분광광도계로 레진의 착색정도를 측정한 결과, 실험군 간의 차이는 포도즙에 침지한 경우 명도가 가장 어두운 것을 볼 수 있었다.

• 한국자원식물학회지
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• 제24권2호
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• pp.195-199
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• 2011

토마토는 세계적으로 매우 중요한 과채류로서, 과실 내에 카로티노이드의 함량이 많아 항암효과, 노화방지 및 비타민이 풍부하다고 알려져 있다. 본 연구에서는 국내외로 부터 수집한 토마토 유전자원 771계통을 대상으로 과색, 과형, 과중, pH, 산도 및 당도 등 주요형질의 분포를 분석하였다. 과색의 분포는 적색과가 약 85%을 차지하며, 복숭아색, 황색, 녹, 오랜지, 백색, 흑색순을 보였다. 과형은 편구가 46%, 정구가 27% 등 다양한 형태로 나타났으며, 과즙당도의 분포범위는 최소 2,2%부터 11,5%까지로 평균 5.6%이었다. 또한 과즙의 산도 분포 범위는 최소 0.124%부터 최대 1.665%까지로, 평균치는 0.881%이었다. 과육중의 라이코핀 함량의 분포범위는 최소 0.0 ${\mu}g/g$부터 80.4 ${\mu}g/g$까지로 평균 43.3 ${\mu}g/g$이었으며, ${\beta}$-카로틴 함량은 최소 1.8 ${\mu}g/g$부터 최대 48.8 ${\mu}g/g$까지로 평균치는 10.8 ${\mu}g/g$이었다. 수집 유전자원의 주요 형질간 상관는 당도와 산도, 산도와 pH, pH와 라이코핀, 라이코핀과 ${\beta}$-카로틴 등이 높은 상관관계를 보였다. 이상의 결과로부터 주요 형질이 높은 유전자원을 이용하여, 고당함유 품종육성, 고 색소함유 품종육성 등 고기능성 토마토 품종육성에 크게 기여 할 것이라고 생각된다.

• 한국균학회지
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• 제27권4호통권91호
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• pp.243-248
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• 1999

야생에서 Cordyceps militaris를 채집하여 실험실에서 균을 분리하였으며, 형태적, 배양적 특징 및 인공자실체 형성조건에 관하며 몇 가지 실험을 하였다. 인공자실체에 형성된 C. militaris의 자좌는 곤봉형 또는 막대 형태로 야생에서와 같이 오랜지색을 띄었다. 자좌에 형성된 자낭각은 달걀모양으로 반나생 하며, 크기는 $30{\sim}90{\times}90{\sim}130\;{\mu}m$로 원주상의 자낭과 장방추형의 자낭포자를 가지고 있었다. C. militaris의 불완전 세대는 구형이나 땅콩모양의 분생포자를 형성하며, PDA 배지에서 양모상으로 중앙 융기한다. 인공자실체 형성조건에 관하며 배양용기로는 생육일수가 빠르고, 발이율과 수량이 높은 PP 배양병이 적당하였으며, 조명도는 1,000 lx에서 자실체 생육이 우수한 결과를 얻었다. 자실체 행성에 적합한 염가의 배지는 누에 번데기 보다 쌀보리, 밀과 현미 등의 곡물배지이며, 자실체 형성을 위해 종균제조에 사용되는 균주는 3회 이상 계대배양 할 경우 급격한 수량 감소의 요인으로 작용하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.34-34
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• 2014

Filamentous fungi of the genus Colletotrichum (teleomorph, Glomerella) are considered major plant pathogens worldwide. Cereals, legumes, vegetables, and fruit trees may be seriously affected by this pathogen (1). Colletotrichum species cause typical disease symptoms known as anthracnoses, characterized by sunken necrotic tissue, where orange conidial masses are produced. Anthracnose appears in both developing and mature plant tissues (2). We investigated disease occurrence in apple orchards from 2013 to 2014 in northern Gyeongbuk province, Korea. Typical anthracnose with advanced symptoms was observed in all apple orchards studied. Of late, static fruit spot symptoms are being observed in apple orchards. A small lesion, which does not expand further and remains static until the harvesting season, is observed at the beginning of fruit growth period. In our study, static symptoms, together with the typical symptoms, were observed on apples. The isolated fungus was tested for pathogenicity on cv. 'Fuji apple' (fully ripe fruits, unripe fruits, and cross-section of fruits) by inoculating the fruits with a conidial suspension ($10^5$ conidia/ml). In apple inoculated with typical anthracnose fungus, the anthracnose symptoms progressed, and dark lesions with salmon-colored masses of conidia were observed on fruit, which were also soft and sunken. However, in apple inoculated with fungi causing static symptoms, the size of the spots did not increase. Interestingly, the shape and size of the conidia and the shape of the appressoria of both types of fungi were found to be similar. The conidia of the two types of fungi were straight and cylindrical, with an obtuse apex. The culture and morphological characteristics of the conidia were similar to those of C. gloeosporioides (5). The conidia of C. gloeosporioides germinate and form appressoria in response to chemical signals such as host surface wax and the fruitripening hormone ethylene (3). In this study, the spores started to germinate 4 h after incubation with an ethephon suspension. Then, the germ tubes began to swell, and subsequently, differentiation into appressoria with dark thick walls was completed by 8 h. In advanced symptoms, fungal spores of virtually all the appressoria formed primary hyphae within 16 h. However, in the static-symptom fungus spores, no primary hyphae formed by 16 h. The two types of isolates exhibited different growth rates on medium containing apple pectin, Na polypectate, or glucose as the sole carbon. Static-symptom fungi had a >10% reduction in growth (apple pectin, 14.9%; Na polypectate, 27.7%; glucose, 10.4%). The fungal isolates were also genetically characterized by sequencing. ITS regions of rDNA, chitin synthase 1 (CHS1), actin (ACT), and ${\beta}$-tubulin (${\beta}t$) were amplified from isolates using primer pairs ITS 1 and ITS 4 (4), CHS-79F and CHS-354R, ACT-512F and ACT-783R, and T1 and ${\beta}t2$ (5), respectively. The resulting sequences showed 100% identity with sequences of C. gloeosporioides at KC493156, and the sequence of the ${\beta}$t gene showed 100% identity with C. gloeosporioides at JX009557.1. Therefore, sequence data from the four loci studied proves that the isolated pathogen is C. gloeosporioides. We also performed random amplified polymorphic DNA-PCR, which showed clearly differentiated subgroups of C. gloeosporioides genotypes. The clustering of these groups was highly related to the symptom types of the individual strains.

• 한국식품조리과학회지
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• 제32권4호
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• pp.433-440
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• 2016

Purpose: To provide the nutritional information of sweet peppers, we have evaluated changes of nutrient content and retention rates by different sweet pepper types and blanching method. Methods: Four types of raw and blanched ($100^{\circ}C$, 30 sec) sweet peppers were prepared and their weight, nutrient content (3 water soluble vitamins, 10 minerals) and retention rates were analyzed. Results: The weight of blanched sweet peppers was decreased than that of raw sweet peppers. From the results of the two-way analysis, the type of sweet pepper had a greater influence than blanching method used on the thiamine, riboflavin, folate and vitamin C contents (but not niacin) (p<0.05). Red pimento contained the most thiamine (0.1 mg/100 g), riboflavin (0.16 mg/100 g), folate ($47.89{\mu}g/100g$) and total niacin (0.11 mg/100 g). Red paprika contained high levels of riboflavin (0.15 mg/100 g) and folate ($44.96{\mu}g/100g$). Orange paprika contained the highest vitamin C content (116.29 mg/100 g). In addition, the mineral content, with the exception of Se, was strongly influenced by the type of sweet pepper rather than the blanching method (p<0.001). The K content of sweet peppers was higher than other minerals. Overall, after blanching, high retention rates (over 70%) were observed in all types of sweet peppers. However, the retention rates of thiamin, vitamin C and Se showed lower levels (less than 70%). Conclusion: The vitamin and mineral contents depends more on the type of sweet pepper; although vitamin and mineral (except Se) contents showed some changes after blanching. Our results can be utilized to determine the effects of cooking processes on nutritional information.

• 동아시아식생활학회지
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• 제7권3호
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• pp.341-354
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• 1997

The purpose of this study was to investigate the food behaviors and food preferences of teenagers by regional groups. The subjects were composed of 365 third grade students in middle schools from urban, rural and fishing areas in Kyungpook province. The data was collected by using a survey questionnaire. The results of the study are summarized as follows: Eating habits of 21-47% of the subjects, according to the regions, were poor and female students in large city showed the best eating habits while female students in fishing village showed the poorest eating habits. Out of the three regional groups studied, the subjects from the fishing villages had the highest rates of skipping meals and eating between meals. Out of all the people investigated, thirty to forty percent had unbalanced diets, the highest percentage coming from the urban areas and the lowest from the fishing villages. All together an allergic reaction to food was the highest in the fishing villages' subjects and female students were more sensitive compared to the male students. Students from the urban area had higher frequency of symptoms of lactose intolerance. Upon the investigation of the subjects 'food preferences, they preferred boiled rice to any other rice as a staple food. Cha jang myun was the most preferred out of all noodles and breads. It was found that teenagers preferred one course meal such as fried rice to a rice-centered regular meal. Out of all various meat soups, beef soup and beef-rib soup were preferred the most. As for subsidiary foods the most preferred were soybean paste stew and cuttlefish stew which they ate daily. There was a very high preference for fried foods, the most favored being pork cutlet followed by fried vegetables, sweet potatoes, and minced meat. The most preferred cooked vegetables were bean sprouts and seasoned cucumber. Seasoned cubed radish roots were the most preferred kind of kimchi. For desserts there was a very high preference for the following fruits: apples, plums, strawberries, and oranges. For beverages the most popular choice were chocolate drinks followed by orange juice, apple juice, milk and tomato juice. According to these investigations, most teenagers liked daily foods which they are used to. The study showed that on the whole the teenagers had poor eating habits. A problem that needs immediate attention is that female students and students from the fishing villages have a high rate of skipping meals. It is expected that the results of this food preference test of teenagers could be useful in the meal planning of middle school lunch programs in the future.

• 한국식품저장유통학회지
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• 제15권3호
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• pp.445-449
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• 2008

국내에서 제조 및 시판되고 있는 사과를 비롯한 과일 주스 5종, 포도 주스 10종의 총 페놀 함량과 항산화 활성을 DPPH 방법과 FRAP 방법으로 조사하였다. 시판 과일 주스 중 오렌지의 총 페놀 함량이 114.09 nlg/L로 가장 높게 나타났고, 포도주스 10종은 $57{\sim}205\;mg/L$의 함량으로 제품 별로 다양하게 나타났다. 과일 주스의 DPPH radical 소거 활성은 자몽이 가장 높았으며 사과, 파인애플이 낮았다. 포도주스의 항산화 활성은 제품에 따라 다양하게 나타났는데, 시료별로 GU4, GU5, GU9가 거의 80%정도의 DPPH radical 소거 활성을 보였다. FRAP방법에 의한 항산화 활성 역시 DPPH 라디칼 소거 활성과 유사한 결과이었다. 그리고 DPPH 및 FRAP, 총 페놀 함량 간의 상관계수는 각각 0.97 이상으로 매우 높은 상관성을 나타내었으며, 따라서 총 페놀 함량이 높을수록 과일 주스 제품의 항산화 활성능력이 크게 나타났다.

• 복식문화연구
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• 제4권2호
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• pp.113-124
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• 1996

This research was designed to find out what is a distictive feature of the design elements of Korean women's costume and its influence by analizing the trend of Korean women's constume during the period of 1890 and 1995. The research was analized by a total of 854 random samples of clothing styles collected from materials in the museums and from articles of three leading newspapers and one of the best women's magazines in Korea. The changing trend of Korean women's costume was found out to be as follows: 1. In around 1910s, the trend of Korean women's costume has been changed into two-tier structure of both Korean and Western styles, and the typical and traditional Korean women's street wears such as 'Chang-ot' and 'Tsdechima' have been gradually diminished. 2. From 1920s, length of the Korean skirt, which was unnecessarily long and inconvenient, has been getting shorter for the convenience of walking. In addition, Korean women's underwear has become simplified, and sports wear could be seen for the first time in Korea. 3. During 1960s, the Western clothing styles have been increased rather than Korean styles as the street wear of the Korean women. 4. In 1970s, blue jeans, mini-skirts and casual wears have become a trend or fashion for women in Korea. 5. In 1980s, a fashion of unisex mode has arrived in this country, and from the year of 1987, wearing of panties among the Korean women has been increased rather than skirts wearing for the street wear. 6. During 1990s, various forma of complex styles came into being in such styles as so-called 'orange class style,' 'protruding navel T-shirts' and 'runner wear speedy.' From mid-1990s, Korean women have turned out to prefer the cute and light costumes by placing emphasis on the image of intellectual, professional and functional wears. The changing special feature of the Korean women's clothing designs could be distinctively observed during 1960s as the modernization policies in Korea have been processed during the period. The clothing trend before 1960 was to change and to solve problems of inconvenient and impractical wears, but the trend after 1960 was seen as a change to confirm a trend of the world fashion. From mid-1990s, however, preference of Korean women's self-conceited conception socially and an unquestionable changing pattern of Korean women's life.

• 한국토양비료학회지
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• 제36권5호
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• pp.311-322
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• 2003

공생균근은 식물 뿌리에 의한 인산 등 무기염류의 흡수를 촉진시키지만 토양의 인산 농도 가 너무 낮거나 높으면 공생균근 형성이 억제되는 것으로 알려져 있다. 본 연구는 화산회토양에서 감귤 뿌리의 공생균근 형성에 적합한 유효인산 수준을 구명하기 위하여 수행하였다. 중과석을 인 공급원으로 하여 인산 시용량을 $0-1000mg\;kg^{-1}$으로 달리한 토양에서 공생균근균 접종과 비접종 처리로 나누어 탱자 유묘를 화분에 심어 유리온실에서 5개월 동안 재배하였다. 탱자 유묘의 공생균근 형성율은 인산 무시용구(유효인산 함량 $0.73mg\;kg^{-1}$)에서 33%이었는데, $50mg\;kg^{-1}$ 시용구(유효인산 함량 $2.6mg\;kg^{-1}$)에서 43%로 증가되었지만 $200mg\;kg^{-1}$ 시용구(유효인산 함량 $8.3mg\;kg^{-1}$)에서는 34%로 무시용구와 비슷하였고 그 이상 인산 시용량이 증가함에 따라 형성율은 오히려 감소하였다. 공생균근균의 접종으로 초장, 생체중 및 건물중 등으로 측정된 탱자 유묘의 생장은 비접종 처리구와 비교하여 모든 인산 처리 수준에서 증가되었으며, 그 증가량은 공생균근 형성율에 비례하였다. 그리고 탱자 유묘의 인산을 비롯한 무기양분 흡수량도 모든 인산 처리 수준에서 공생균근 형성율과 높은 정의 상관관계를 보였다.