• Archives of Pharmacal Research
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• 제21권3호
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• pp.353-356
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• 1998

Geraniol (1), olivetol (2), cannabinoids (3 and 4) and 5-fluorouracil (5) were tested for their growth inhibitory effects against human oral epitheloid carcinoma cell lines (KB) and NIH 3T3 fibrobalsts using two different 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. Cannabigerol (3) exhibited the highest growth-inhibitory activity against the cancer cell lines.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2036-2042
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• 2016

We evaluated the potentials of 10 isothiocyanates (ITCs) from cruciferous vegetables and radish root hydrolysate for inhibiting the growth of oral pathogens, with an emphasis on assessing any structure-function relationship. Structural differences in ITCs impacted their antimicrobial activities against oral pathogens differently. The indolyl ITC (indol-3-carbinol) was the most potent inhibitor of the growth of oral pathogens, followed by aromatic ITCs (benzyl ITC (BITC) and phenylethyl ITC (PEITC)) and aliphatic ITCs (erucin, iberin, and sulforaphene). Sulforaphene, which is similar in structure, but has one double bond, showed higher antimicrobial activity than sulforaphane. Erucin, which has a thiol group, showed higher antimicrobial activity than sulforaphane, which has a sulfinyl group. BITC and iberin with a short chain exhibited higher antimicrobial potential than PEITC and sulforaphane with a longer chain, respectively. ITCs have strong antimicrobial activities and may be useful in the prevention and management of dental caries.

• International Journal of Oral Biology
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• 제44권3호
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• pp.101-107
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• 2019

The purpose of this study was to investigate the antimicrobial activity of the ethanol extract of Garcinia mangostana L. (mangosteen) against Cutibacterium acnes (6 strains) and Staphylococcus aureus (6 strains). The antimicrobial activity of the mangosteen extract was evaluated based on its minimal bactericidal concentration. Cytotoxicity of the mangosteen extract against human embryonic kidney 293 (HEK 293) cells was determined using the cell counting method. The data showed that the mangosteen extract was not toxic to HEK 293 cells at a concentration of up to $16{\mu}g/mL$ and killed 87.0% and 99.9% of C. acnes and S. aureus after 10 minutes and 1 hour of treatment, respectively. These results suggest that ethanol extract of mangosteen can be used as an anti-acne agent.

• International Journal of Oral Biology
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• 제46권1호
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• pp.45-50
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• 2021

This study evaluated the antimicrobial activity of Endoseal TCS, an mineral trioxide aggregate-based root canal sealer, mixed with water-soluble mangostin derivatives (WsMD) of Garcinia mangostana L. (mangosteen) ethanol extract against Enterococcus faecalis and Staphylococcus aureus. The antibacterial activity of Endoseal TCS mixed with WsMD against three strains of E. faecalis and three strains of S. aureus was performed using agar diffusion test. The data showed that Endoseal TCS mixed with 0.115% WsMD had a zone of inhibition of 0.7 ± 0.2-2.4 ± 0.1 mm. The results suggest that Endoseal TCS mixed with WsMD of Garcinia mangostana L. ethanol extract is useful as a root canal sealer with antibacterial activity against E. faecalis and S. aureus.

• 한국치위생학회지
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• 제12권6호
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• pp.1203-1211
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• 2012

Objectives : The aim of this study was to investigate the factor-related oral care self-efficacy among the type 2 diabetic patients. Methods : Questionnaire was conducted with 174 Type 2 diabetic patients from 9th January to 9th March in 2012. The following conclusion was obtained as a result of carrying out t-test and one-way ANOVA analysis and multiple linear regression analysis. Results : 1. Analysis of the level of each item concerning oral care self-efficacy showed tooth brushing self-efficacy was $13.3{\pm}2.9$, the highest of all. 2. The factor that was most highly related with oral care self-efficacy was oral health behaviors(${\beta}=0.474$). The other factors were found to be expected duration of diabetes(${\beta}=-0.205$), self-assessed physical health(${\beta}=0.177$) and oral health(${\beta}=0.111$) in such order (p<0.05). Conclusions : Diabetes causes a variety of complications in the mouth, and therefore it is very important to practice oral care activity in order to oral health promotion. This study showed oral care self-efficacy appeared to be the greatest factor of relevance in practicing oral care activity. So, dental hygienist is obliged to keep on motivating so that the patient may maintain the oral care activity for him/herself. Also, a study on various intervention methods to improve oral care self-efficacy should be continued.

• Journal of Oral Medicine and Pain
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• 제15권1호
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• pp.27-35
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• 1991

In order to evaluate muscular activity patterns of masticatory muscles and asymmetry patterns of muscular activity according to the levels of occlusal force, twenty-one individuals of age ranged from 23 to 27 years were examined. They were selected according to the following criteria : 1) no symptoms of temporomandibular disorder, 2) complete dentition except third molars, 3) normal or Angle's class I molar relationship and 4) no experience of dental treatment. The electromyographic amplitudes was measured for evaluation of muscular activity and asymmetric patterns of masseter and anterior temporal muscle during unilateral clenching at the levels of 10%, 20%, 40% and 50% of the maximum occlusal force by use of electromyogram and bite force meter. The obtained results were as follows : 1. The muscle activity indices of masticatory muscles of clenching side at the clenching levels of 10%, 20% and 30% of the maximum occlusal force were -19.12, -9.87, -0.49%, so that activity of anterior temporal muscle was dominant than that of masseter muscle. At the levels of 40%, 50% of the maximum occlusal force, muscle activity indices were 4.68%, 6.70%, so that activity of masseter was dominant at all level and as the levels of occlusal force was increased, muscular activity index was tend to decrease. 2. In masseter, asymmetry indices of muscular activity at the levels of 10%, 20% of maximum occlusal force were -10.34 and -1.24%, so that muscular activity of non-clenching side were dominant and at the levels of 30%, 40% and 50% each of maximum occlusal force, muscular activity was dominant on clenching side as 4.68, 7.18 and 10.9%. In anterior temporal muscle, asymmetry indices were 33.38%, 25.46, 2095, 10.23 and 15.45% at the levels of 10%, 20%, 30%, 40% and 50% each of maximum occlusal force, so that activity of clenching 15.45% at the levels of 10%, 20%, 30%, 40% and 50% each of maximum occlusal force, so that activity of clenching side was dominant than that of non-clenching side at all levels, but as the levels of occlusal force was increased, asymmetry indices of muscular activity was tend to decrease. 3. Between both sides, average electromyographic amplitudes of masseter and anterior temporal muscle were correlated, so that as the levels of occlusal force was increased, average electromyographic amplitudes of both side in same muscle were increased proportionally. But asymmetry indices between muscular activities of masseter and anterior temporal muscle were not correlated.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제44권2호
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• pp.79-85
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• 2018

Objectives: The aim of this study was to evaluate the effects of herbal extracts on bone regeneration. Two known samples were screened. Materials and Methods: We previously established a rat calvaria defect model using a combination of collagen scaffold and herbal extracts. An 8 mm diameter trephine bur with a low-speed dental hand piece was used to create a circular calvaria defect. The experimental group was divided into 4 classifications: control, collagen matrix, Danshen with collagen, and Ge Gan with collagen. Animals in each group were sacrificed at 4, 6, 8, and 10 weeks after surgery, and bone regeneration ability was evaluated by histological examination. Results: Results revealed that both Danshen and Ge Gan extracts increased bone formation activity when used with collagen matrix. All groups showed almost the same histological findings until 6 weeks. However, after 6 weeks, bone formation activity proceeded differently in each group. In the experimental groups, new bone formation activity was found continuously up to 10 weeks. In the Danshen and Ge Gan groups, grafted materials were still present until 10 weeks after treatment, as evidenced by foreign body reactions showing multinucleated giant cells in chronic inflammatory vascular connective tissue. Conclusion: Histological analyses showed that Danshen and Ge Gan extractions increased bone formation activity when used in conjunction with collagen matrix.

• International Journal of Oral Biology
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• 제30권4호
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• pp.125-130
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• 2005

Thrombin-induced platelet microbicidal proteins (tPMP) are antibacterial proteins released when platelets are stimulated by thrombin. It has been reported that tPMP has antibacterial activity against various bacterial species including causative agents of infective endocarditis. Most of the oral streptococci have resistance to the killing by tPMP and this fact may play an important role as a virulence factor in infective endocarditis. However, the susceptibility and resistance mechanism of oral streptococci for tPMP have not been revealed yet. In this study, the killing mechanism of tPMP for oral streptococci has been investigated. Streptococcus rattus BHT, a susceptible strain, and Streptococcus gordonii DL1, a resistant strain, have been used in this study. tPMP was isolated from platelet after stimulation with thrombin. Cell membrane depolarization was examined with 3,3'-dipropylthiodicarbocyanine iodide ($DiSC_3$), membrane potential-sensitive cyanine dye, by fluorescence spectrophotometry. The permeabilization of cell membrane by tPMP was investigated with propidium iodide (PI) by flow cytometry. tPMP susceptible S. rattus BHT showed the increase of the $DiSC_3$ fluorescence level meaning depolarization of cell membrane and increase of the uptake of PI which means permeabilization of cell membrane. However, tPMP resistant S. gordonii DLI did not show depolarization and permeabilization. These results indicate that the increasing depolarization and permeabilization of oral streptococcal cell membrane are associated with the bactericidal activity of tPMP.

• 동의생리병리학회지
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• 제17권2호
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• pp.574-579
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• 2003

Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important hospital and community pathogens. Therefore, new agents are needed to treat the MRSA. In the present study, we investigated antimicrobial activity of ethyl acetate, methanol, and water extracts of Curcuma longa L. (C. longa) aganist clinical isolates of MRSA. The ethyl acetate extract of C. long a demonstrated a higher antibacterial activity than the methanol extract or water extract. Since the ethyl acetate extract was more active than other extracts, we examined whether ethyl acetate extract may restore the antibacterial activity of β-lactams and alter the adhesion and invasion of MRSA to human mucosal fibroblasts (HMFs). In the checkerboard test, ethyl acetate extract of C. longa markedly lowered the MICs of ampicillin and oxacillin against MRSA. In the bacterial adhesion and invasion assay, MRSA intracellular invasion were notably decreased in the presence of 0.125 - 2 mg/ml of C. longa extract compared to the control group. These results suggest that ethyl acetate extract of C. longa may have antibacterial activity and the potential to restore the effectiveness of β-lactams against MRSA, and inhibit the MRSA adhesion and invasion to HMFs.

• 한국융합학회논문지
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• 제13권4호
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• pp.127-135
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• 2022

본 연구는 한국중년여성의 경제활동여부가 삶의 질과 구강건강에 미치는 융합적인 영향을 확인하기 위한 기초자료로 제공하고자 한다. 2019년 국민건강영양조사 자료를 사용하였고, 경제활동 여부가 구강건강관련 요인과 건강관련 삶의 질 (Korean Health-related Quality of Life Instrument with 8 Items; HINT-8)에 미치는 영향을 확인하기위해 복합표본 로지스틱 회귀분석과 선형회귀분석을 시행하였다. 그 결과, 인구학적 특성을 통제하고, 경제활동을 하는 경우 HINT-8의 하위항목 중 4개의 항목(기운, 일하기, 기억, 잠자기)에서 유의한 영향이 있었다. 구강건강 상태를 보정한 경우 HINT-8의 2개의 항목(일하기, 기억)에서만 유의한 영향이 있었다(p<.05). 따라서 한국 중년여성의 경제활동이 삶의 질에 긍정적인 영향을 미치지만 구강건강상태가 그 영향요인을 감소시킬 수 있다는 점에서 그 가치가 있다고 생각된다.