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Virulence factors and multi-drug resistant patterns of pathogenic Escherichia coli isolates from diarrheic calves in Jeonbuk (전북지역 송아지 설사 유래 병원성 대장균의 병원성 인자 및 다제 내성 패턴)

  • Kwak, Kil-Han;Kim, Seon-Min;Yu, Yeong-Ju;Yu, Jeong-Hee;Lim, Mi-Na;Jang, Yu-Jeong;Hur, Jin
    • Korean Journal of Veterinary Service
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    • v.44 no.4
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    • pp.271-281
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    • 2021
  • Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.

Analysis on the Characteristics of Academic Achievement of Middle School Students About 'composition of matter': Focusing on the Results of the National Assessment of Educational Achievement (NAEA) (중학생들의 '물질의 구성' 영역 학업성취 특성 분석 : 국가수준 학업성취도 평가 결과를 중심으로)

  • Baek, Jongho;Lee, Jae Bong;Choi, Wonho
    • Journal of the Korean Chemical Society
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    • v.66 no.2
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    • pp.136-149
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    • 2022
  • Chemistry focuses on explaining macroscopic phenomena at the microscopic level with particles, such as atoms or molecules. Explanation using particles are bound to be considered as abstract by students, because it was dealing with invisible objects. For that reason, the science national curriculum presented to middle school students the explanation of the units related to the composition of matter. Therefore, understanding about the composition of matter in middle school students becomes an important basis for learning of chemistry, and it is necessary to investigate their understanding about composition of matter. In this study, students' understanding about 'composition of matter' region, which is first presented to middle school students, was confirmed at an overall level. In this line, this study analyzed the results of the items in the composition of matter region, and analyzed items were used in the National Assessment of Educational Achievement (NAEA) from 2015 to 2019. We analyzed the 9 items presented in the NAEA according to the response rate of options and response rate distribution curve, and explained the characteristics of understanding derived by each achievement level were examined. According to the analyzed results by dividing the conceptions about elements, atoms, and ions, students above the proficient achievement-level had scientific conceptions overall, but students below the basic achievement-level had inconsistent or naive conceptions. Based on the results for each item, this study discussed some implications to be considered or to be improved on teaching-learning for 'composition of matter'.

Spatial Assessment of Climate Suitability for Summer Cultivation of Potato in North Korea (기후적합도 모형을 활용한 북한지역 내 감자의 여름재배 적지 탐색)

  • Kang, Minju;Hyun, Shinwoo;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.1
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    • pp.35-47
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    • 2022
  • Expansion of potato production areas can improve the food security in North Korea because the given crop has less requirements for agricultural materials and facilities. The Global Agro-Ecological Zones (GAEZ) model, which was developed to evaluate climate suitability under different cultivation conditions, was used to identify potential areas for the potato production. The spatial estimates of crop suitability under low and high input management conditions were downloaded from the GAEZ data portal. The values of suitability were obtained at the potato occurrence sites retrieved from the Global Biodiversity Information Facility (GBIF) database. The suitable areas for the potato production were identified using a threshold value derived from the suitability estimates at the occurrence sites. It was found that 90% of the occurrence sites had the suitability index value >3,333, which was set to be the threshold value. The suitable areas in North Korea were summarized by province and county. Rice cultivation areas were excluded from the analysis. The reported relative acreage of potato production was better represented by the suitable areas under the low input management options than the high input conditions. The suitable areas also had a similar distribution to the reported acreage of potato production by county. These results indicated that the GAEZ model would be useful to identify the candidate production areas, which would facilitate the increases in potato production especially under future climate conditions. Furthermore, monthly maps of crop suitability can be used to design cropping systems that would improve crop production under the limited use of agricultural materials and facilities.

Delphi Survey for COVID-19 Vaccination in Korean Children Between 5 and 11 Years Old (국내 5-11세 소아의 코로나19 백신 접종에 대한 델파이 연구)

  • Choe, Young June;Lee, Young Hwa;Choi, Jae Hong
    • Pediatric Infection and Vaccine
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    • v.29 no.1
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    • pp.37-45
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    • 2022
  • Purpose: During the coronavirus disease 2019 (COVID-19) pandemic, we conducted a Delphi survey that included the experts from the field of COVID-19 immunization in children aged 5-11 years. The aim was to organize collective expert opinions on COVID-19 vaccination in young children in the Republic of Korea, and so thus assist the vaccination policy. Methods: The panels included pediatric infectious disease specialists, preventive medicine experts, infectious disease physicians, and COVID-19 vaccine experts consulting the Ministry of Health and Welfare. The Delphi survey was conducted online using a questionnaire from February 14 to February 27, 2022. Results: The Delphi panels agreed that children were vulnerable to COVID-19, and the severity of illness was modest. Furthermore the panels reported that children with chronic illness were more susceptible to a worsening clinical course. There were generally positive opinions on the effectiveness of COVID-19 vaccination in children aged 5-11 years, and experts gathered a slightly positive opinion that the adverse events of pediatric COVID-19 were not numerous. The benefits of COVID-19 vaccination were evaluated at a level similar to the potential risks in children. Currently, the only approved mRNA platform vaccine in children seemed to be sustainable; however, the recombinant protein platform COVID-19 vaccines were evaluated as better options. Conclusions: Due to the surge of the Omicron variant and an increase in pediatric cases, the COVID-19 vaccination in young children may have to be considered. Panels had neutral opinions regarding the COVID-19 vaccination in children aged 5-11 years. Thus monitoring of the epidemiology and the data about the safety of COVID-19 vaccination should be continued.

Development and Application of Assessment Items for the Diagnosis of Difficulties in Learning Elementary Mathematics (초등학교 수학 학습 어려움 진단을 위한 평가 문항 개발 및 적용 연구)

  • Kim, Hee-jeong;Cho, Hyungmi;Ko, Eun-Sung;Lee, Donghwan;Cho, Jinwoo;Choi, Jisun;Han, Chaereen;Hwang, Jihyun
    • Journal of the Korean School Mathematics Society
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    • v.25 no.3
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    • pp.261-278
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    • 2022
  • The purpose of this study is to develop an assessment to diagnose difficulties in learning mathematics and misconstructions that elementary students have. With thorough theoretical background and analysis of mathematics curriculum documents, we established learning trajectories for the following content areas in grades 3 to 6: number and operation, regularity, data and chance, geometry, and measurement. Then, the research team created the assessment items targeting a specific stage in the learning trajectories and including item options to identify possible misconceptions. Based on the unified validity theory, we reported the detailed procedure of the assessment development and the evidence for the content, substance, and structural validity of the assessment. We collected the data of 675 elementary students. Rasch measurement modeling was applied, and Cronbach's alpha was estimated. We considered how to report students' assessment results to teachers appropriately and immediately, which suggested important implications for supporting teaching and learning mathematics in elementary schools. We also suggested how to use the assessment developed in this study in online and distance learning environments due to the COVID-19 pandemic.

Analysis of Anti-Reversing Functionalities of VMProtect and Bypass Method Using Pin (VMProtect의 역공학 방해 기능 분석 및 Pin을 이용한 우회 방안)

  • Park, Seongwoo;Park, Yongsu
    • KIPS Transactions on Computer and Communication Systems
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    • v.10 no.11
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    • pp.297-304
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    • 2021
  • Commercial obfuscation tools (protectors) aim to create difficulties in analyzing the operation process of software by applying obfuscation techniques and Anti-reversing techniques that delay and interrupt the analysis of programs in software reverse engineering process. In particular, in case of virtualization detection and anti-debugging functions, the analysis tool exits the normal execution flow and terminates the program. In this paper, we analyze Anti-reversing techniques of executables with Debugger Detection and Viralization Tools Detection options through VMProtect 3.5.0, one of the commercial obfuscation tools (protector), and address bypass methods using Pin. In addition, we predicted the location of the applied obfuscation technique by finding out a specific program termination routine through API analysis since there is a problem that the program is terminated by the Anti-VM technology and the Anti-DBI technology and drew up the algorithm flowchart for bypassing the Anti-reversing techniques. Considering compatibility problems and changes in techniques from differences in versions of the software used in experiment, it was confirmed that the bypass was successful by writing the pin automation bypass code in the latest version of the software (VMProtect, Windows, Pin) and conducting the experiment. By improving the proposed analysis method, it is possible to analyze the Anti-reversing method of the obfuscation tool for which the method is not presented so far and find a bypass method.

Detection of the Carbapenem Resistance Gene in Gram-negative Rod Bacteria Isolated from Clinical Specimens (임상검체에서 분리된 그람음성막대균으로부터 카바페넴 내성 유전자 검출)

  • Yang, Byoung Seon
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.3
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    • pp.179-191
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    • 2022
  • Carbapenem-resistant Enterobacteriaceae (CRE) poses an increasing public health threat and has limited treatment options with high associated mortality. Genotypes of carbapenemase that threaten public health (blaKPC, blaNDM, blaIMP, and blaVIM) and blaOXA-48-like genes were detected by phenotypic and molecular diagnosis, and related gene distribution patterns were investigated. Phenotypic testing using the modified Hodge test confirmed positivity in all 41 strains examined, and carbapenemase inhibitory testing using meropenem+phenyl boronic acid or meropenem+EDTA confirmed positivity in 18 and 8 strains, respectively. Polymerase chain reaction revealed the presence of amplification products in 28 strains of blaKPC, 25 strains of blaNDM, 5 strains of blaIMP, 1 strain of blaVIM, and 13 blaOXA-48-like strains. In addition, 7 strains of blaKPC+blaNDM, 1 strain of blaKPC+blaIMP, 1 strain of blaNDM+blaOXA-48-like, 1 strain of blaNDM+blaVIM, 4 strains of blaKPC+blaNDM+blaIMP, and 4 strains of blaKPC+blaNDM+blaOXA-48-like were identified. Melting curve analysis using real-time PCR was wholly consistent with PCR results. The study shows genetic identification of highly specific CRE by real-time PCR could be used to provide early diagnoses and infection control, improve surveillance, and prevent the transmission of CRE.

Implant-assisted removable partial denture using Milled-Bar and Attachment in partially edentulous maxilla: A case report (상악 부분 무치악 환자에서 Milled-bar와 어태치먼트를 이용한 임플란트 부분 피개의치 수복 증례 보고)

  • Jeong, Yun-Su;Jeong, Chang-Mo;Yun, Mi-Jung;Lee, So-Hyoun;Huh, Jung-Bo
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.4
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    • pp.412-419
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    • 2022
  • For treatment of partially edentulous patients, a treatment using implant is widely used. Treatment method using implant are implant fixed prostheses and removable partial dentures, and for patients with severe bone resorption, removable implant overdenture with the effects of aesthetic and reducing cost can be used as treatment options. Specially, prosthesis with milled-bar and attachment has the effect of being splinted between implant fixtures, higher retention and stability than conventional removable partial denture. And it has the effect of improvement of aesthetic through lip support by denture base. In this case, the patient with severe alveolar bone resorption and partial edentulous maxilla and mandible was treated by implant-assisted removable partial denture using Milled-bar and ADD-TOC attachment. The esthetic was improved by removing the clasp because of effects of additional retention by using the attachment, and reducing palatal coverage of implant-assisted removable partial denture. The clinical results were satisfactory on the aspect of aesthetic and masticatory function.

A Study on the Management of Non-Communicable Disease in Fiji (피지에서의 만성병 관리)

  • Kim, Daeseon;Romakin, Pablo;Rafai, Eric;Lee, Chulwoo
    • Journal of Appropriate Technology
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    • v.6 no.2
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    • pp.163-173
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    • 2020
  • For the successful execution of an ODA project, it is necessary to know what areas are weak and necessary to the country of demand exactly. The health sector is also a top priority in most of developing countries. This study was carried out to introduce non-communicable disease (NCD) in Fiji for ODA projects planning. The major causes of death in Fiji in 2016 are diabetes, ischemic heart disease, cerebrovascular disease, chronic kidney disease, lower respiratory infect, asthma in ranking. The major causes of death in Korea in same year are cancer, ischemic heart diseases, cerebrovascular diseases, pneumonia, suicide, diabetes in the order of ranking. The chronic disease as non-communicable disease (NCD) has been increasing continuously due to changes in lifestyle and consumption patterns and population aging in prevalence rate. This global trend is also apparent in Fiji and Korea, reflected in increasing mortality and personal costs for the treatment and management of NCD. The need for a sustained comprehensive treatment tailored for individual patients has suggested from many studies and the development of a systematic program to manage NCD patients to provide such care have been recommended. The Fiji government developed Non-communicable Diseases Strategic Plan 2015-2019 and has tried to reduce the prevalence rate of non-communicable diseases by factors. The WHO global action plan guiding national-level NCD policies requires an NCD prevention and control model at the community level, presenting strategic goals and detailed options for the introduction and application of the approach to communities. It is necessary to develop an NCD prevention and control model, consisting of a strategy of community intervention, education for students and NCD patients, and the legal enactment of NCD that adequately meets the needs of community members.

A Study on the Occupation Performance of the Leader of Fitness Club by Emotional Labor (피트니스 클럽 지도자의 감정노동에 의한 직업성과에 관한 연구)

  • Yoon, Jae-Soon
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.8
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    • pp.381-390
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    • 2020
  • This purpose of study was to influence of fitness club leader's emotional labor on job involvement and occupation performance. This study aims at providing fundamental data and information on fitness club leader's emotional labor. The survey was done through 250 copies and excluding 23 copies ran an analysis on the remaining 227(90.8%) copies. After question investigating the data which is collected used IBM SPSS statistics 21 and IBM AMOS 21 program, frequency analysis, Exploratory factor analysis and confirmatory factor analysis, convergent validity, discriminant validity, Cronbach's α, correlation analysis, path analysis through Structural Equation Model(SEM). The result of this study were as follows. First, fitness club leader's emotional labor showed (-) effects on job involvement. Second, fitness club leader's emotional labor showed (-) effect on occupation performance. Third, job involvement showed (+) effects on occupation performance. Fitness instructors have severe emotional labor. Therefore fitness instructors manage their emotional labor. This can increase job commitment and job performance. For teeth manager need to increase support options from fitness instructors.