• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.292-295
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• 1990

The bacterial strain KH-1167, identified as Ctoetridiunt sp. was found to produce the antibiotics KG-1167A & KG-1167B which showed a broad spectrum antibacterial activity. The highest antibiotics production was obtained in fermentation medium containing glucose, casamino acid and salt complex solution. Optimum culture condition for the maximum production of the antibiotics was also determined. Antibiotic productivity of the Clostridium sp. KH-1167 was increased to about 450% compared to that in the medium used for the primary isolation procedure.

• Applied Biological Chemistry
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• v.47 no.3
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• pp.287-292
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• 2004

The optimum conditions for lignin peroxidase production were studied. Lignin peroxidase was produced almost exclusively in stationary culture with the optimum media composition of malt extract 1 g, yeast extract 0.4 g, glucose 0.4 g and distilled water 100 ml. Tween 80 at 0.005% concentration and veratryl alcohol at 0.4 mM were very effective inducers for lignin peroxidase production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.671-676
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• 2011

To determine the optimum light intensity for mass culture of the brackish-water cyclopoid copepod Paracyclopina nana, survival, growth, and productivity of the copepod were examined at several light intensities (0, 10, 100, 500, 1,000 lx). The survival rate of P. nana from nauplius to adult decreased with increasing light intensity. The highest survival rate was found under the dark condition, with 61.7% surviving; no significant difference was observed between 0 and 10 lx (51.7%) and the lowest survival rate was with 100 lx (26.7%). Survival rates at 500 and 1,000 lx were significantly lower in comparison with other conditions. The developmental period from nauplius to copepodid (5.8 days) and to adult (11.8 days) at 10 lx was significantly shorter than in the other treatments. Daily mean nauplius production of adult females over 7 days at 0, 10 and 100 lx was significantly higher than at 500 and 1,000 lx. In the 1,000 lx treatment, 99% of the adult females died on the $14^{th}$ day. The optimum light intensity for the mass culture of P. nana could be 10 lx, which had no adverse effects on survival, development, or reproduction.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.131-135
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• 1997

Several microorganisms capable of producing protopectinase, which catalyzes dissociation of plant tissue to single cells, were isolated from soils. Crude enzymes prepared from culture supernatants of the strains released potato cells as a single cell from potato tissues. One of the isolated strains showing higher activity of protopectinase was selected and identified as Rhizopus sp. from the morphological characteristics. For preparing single cells from potato tissues, optimum enzyme activity of protopectinase, which was prepared from culture filtrate of Rhizopus sp. R2, was obescrved at $40^{\circ}C$ and pH 4.

• Journal of Microbiology
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• v.45 no.3
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• pp.199-204
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• 2007

The objective of this study was to evaluate the effect of soluble carbohydrates (glucose, cellobiose), pH (6.0, 6.5, 7.0), and rumen microbial growth factors (VFA, vitamins) on biohydrogenation of linoleic acid (LA) by mixed rumen fungi. Addition of glucose or cellobiose to culture media slowed the rate of biohydrogenation; only 35-40% of LA was converted to conjugated linoleic acid (CLA) or vaccenic acid (VA) within 24 h of incubation, whereas in the control treatment, 100% of LA was converted within 24 h. Addition of VFA or vitamins did not affect biohydrogenation activity or CLA production. Culturing rumen fungi at pH 6.0 slowed biohydrogenation compared with pH 6.5 or 7.0. CLA production was reduced by pH 6.0 compared with control (pH 6.5), but was higher with pH 7.0. Biohydrogenation of LA to VA was complete within 72 h at pH 6.0, 24 h at pH 6.5, and 48 h at pH 7.0. It is concluded that optimum conditions for biohydrogenation of LA and for CLA production by rumen fungi were provided without addition of soluble carbohydrates, VFA or vitamins to the culture medium; optimum pH was 6.5 for biohydrogenation and 7.0 for CLA production.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.279-285
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• 2002

Phytase (myo-inositol hexakisphosphate phospho-hydrolase, EC 3.1.3.8) catalyzes the hydrolysis of phytate (myo-inositol hexakisphosphate) to release inorganic phosphate. A bacterial strain producing phytase was isolated from soil around a cattle shed. To identify the strain, cellular fatty acids profiles, the GC contents, a quinine-type analysis, and physiological test using an API 20NE kit were carried out. The strain was identified to be a genus of Pseudomonas sp. and named as Pseudomonas sp. YH40. The optimum culture condition for the maximum productivity of phytase by Pseudomonas sp. YH40 were attained in a culture medium composed of $1.0\%$ (w/v) glycerol, $2.0\%$ (w/v) peptone, and $0.2\%$ (w/v) $FeSO_4{\cdot}7H_2O$. Within the optimal medium condition, the production of phytase became highest after 10 h of incubation, and the maximal phytase production by Pseudomonas sp. YH40 was observed at $37^{\circ}C$ and pH 6.0.

• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.560-566
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• 1996

A strain capable of producing thermostable chitinase suitable for chitooligosaccharide production was isolated from high temperature environment and identified as Bacillus licheniformis. The chitinase from Bacillus licheniformis KFB-Cl4 was only induced by addition of colloidal thitin into the basal medium as carbon source, showing the decrease of the chitinase production by supplernental addition of other carbon sources into the medium containing 1.0% colloidal chitin. Among organic and inorganic nitrogen sources, yeast extract was the most effective for the increase of total activity and specific activity, and had high affinity for the enzyme production. The optimum temperature of cell growth and thermostable chitinase production was 55$\circ$C. The optimum culture medium was composed of 1.2% colloidal chitin, 0.15% K$_{2}$HPO$_{4}$, 0.05% KH$_{2}$PO$_{4}$, 0.01% MgSO$_{4}$-7H$_{2}$O, 0.1% yeast extract (pH 6.5). Bacillus licheniformis KFB-C14 produced the thermostable chitinase of 3.89 units per ml culture fluid and 7.4 units per mg protein under rotary shaking at 150 rpm for 40 hr.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.235-238
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• 2000

The production of polysaccharide from Paecilomyces japonica was studied in the shake flask culture. For the cell growth and the polysaccharide production, the optimum synthetic medium was glucose peptone(YMP) medium. The flask culture conditions for the polysaccharide production were $27^{\circ}C$ and 200rpm with the initial pH 9 for 8days cultivation.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.386-389
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• 2001

This study was concentrated on the variation of cordycepin production from liquid culture of Paecilomyces japonica.. Cordycepin production was measured from mycelium and culture broth of P. japoniaz. Optimum media composition was studied on inorganic N-sources, inorganic salts, trace elements and C/N ratio. In this results, cordycepin production in medium containing $(NH_4)_2HPO_4$, histidine and $CaCO_3$ was higher than control in culture broth.

• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.75-81
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• 1997

Streptomyces chibaensis J-59 did not grow in the culture medium containing only xylose or xylan as a carbon source, because it was defective in xylulokinase production; xylB mutant. S. chibaensis J-59 was able to produce xylanase and $\beta $-xylosidase as well as glucose isomerase. The glucose isomerase in S. chilbaensis J-59 was induced in the medium containing xylan or xylose which could be utilized as an inducer but not sa carbon and energy sources. So we tried to produce glucose isomerase whthout consumption of xylose or xylan as an inducer by using xylB mutant S. chilbaensis J-59. The optimum condition for the production of the glucose isomerase was attained in a culture medium composed of 1% xylan, 0.15% glucose, 1.5% corn steep liquor, 0.1% MaSO$_{4}$ $\CDOT $7H$_{2}$O, and 0.012% CoCL$_{2}$ $\CDOT $ 6H$_{2}$O(pH 7.0). The production of the enzyme reached to a maximum level when the bacteria were cultured for 42 h at 30$\circ $C. The enzyme production in a jar fermentor was increased twice as much as that in a flask culture.