Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Rhizopus sp. R2 Producing Protopectinase and Optimum Condition ofr Pre-paring Single Cells from Potato Tissues

Protopectinase 생산균주, Rhizopus sp. R2의 분리 및 감자조직의 단세포화를 위한 최적조건

  • 이승철 (경남대학교 공과대학 식품공학과) ;
  • 고보성 (경남대학교 공과대학 식품공학과) ;
  • 김향미 (경남대학교 공과대학 식품공학과) ;
  • 김기운 (경남대학교 공과대학 식품공학과) ;
  • 황용일 (경남대학교 공과대학 식품공학과)
  • Published : 1997.06.01
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Abstract

Several microorganisms capable of producing protopectinase, which catalyzes dissociation of plant tissue to single cells, were isolated from soils. Crude enzymes prepared from culture supernatants of the strains released potato cells as a single cell from potato tissues. One of the isolated strains showing higher activity of protopectinase was selected and identified as Rhizopus sp. from the morphological characteristics. For preparing single cells from potato tissues, optimum enzyme activity of protopectinase, which was prepared from culture filtrate of Rhizopus sp. R2, was obescrved at $40^{\circ}C$ and pH 4.

식물조직을 분해하여 단세포화하는 효소, protopectinase를 생산하는 다수의 미생물을 토양에서 분리하였다. 이들 분리된 미생물의 배양여액에서 얻어진 조효소를 감자조직에 처리하여 단세포화된 감자세포를 확인할 수 있었다. 이들 미생물 중에서 비교적 높은 protopectinase의 효소활성을 보이는 균주를 선택하여 형태관찰 결과로부터 Rhizopus sp.임을 알 수 있었다. 선별된 균주 Rhizopus sp. R2의 배양여액에서 얻어진 protopectinase를 이용하여 감자조직의 단세포화를 시도하여 $40^{\circ}C$, pH 4 하에서 최적의 효소반응 조건을 보였다.

Keywords

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