• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.315-316
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• 2001

In this study various nutritional and environmental parameters such as, initial moisture content, pH. inoculum size, air rate, sample size and nutrient supplement that influence pigment production were evaluated in solid-state cultures. optimum initial moisture content and pH were determined to be 50% and 6.0, respectively. The supplement of the substrate with different carbon, nitrogen, and mineral source reveals a more inhibitive effect as the substrate concentration increase. optimum aeration rate was determined to be 2vvm in flask culture. The maximum amount of red pigment, 3500 OD/g dried fermented rice, was obtained in optimum conditions which is obtained in solid flask culture.

• KSBB Journal
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• 제23권3호
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• pp.199-204
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• 2008

Culture conditions for biological hydrogen production were investigated in wastewater of Takju manufacturing factory. Rhodobacter spaeroides KCTC1425, photosynthesis bacteria, and Enterobacter cloacae YJ-1, anaerobic bacteria were used. The hydrogen production were $195.3m{\ell}{\cdot}H_2/{\ell}$ broth for Rhodobacter spaeroides KCTC1425 and $271.8m{\ell}{\cdot}H_2/{\ell}$ broth for Enterobacter cloacae YJ-1 during 36 h. The hydrogen production increased with light intensity, and were highest over 12000Lux. In mixed culture of Rhodobacter spaeroides KCTC1425 and Enterobacter cloacae Y J-1, the optimum mixing ratio of hydrogen production was 20 and 80. Adding volume of yeast extract for maximum hydrogen production was 15 $g/{\ell}$, but there was no effect over that. $Na_2MoO_4$ was most effective among the inorganic salts, and the optimum volume was 0.4 $g/{\ell}$. In semi-continuous culture, total hydrogen production was $13086m{\ell}{\cdot}H_2/{\ell}$ broth for 144 h with operating period of 24 h.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.131-135
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• 2002

Acetobacter xylinum KJ1 efficiently producing bacterial cellulose(BC) in shaking culture was isolated from a rotten grape. The strain was used to investigate optimum operating conditions for increasing BC production and factorial design model was employed for the optimization. The results of experiments were statistically analyzed by SAS program. Reciprocal effects of each factors(carbon source concentration, shaking speeds(rpm), oxygen pressure, and CSL concentration) and culture condition of BC production were examined by getting regression equation of the dependent variable. Comparisons between experimental results and predicted results about BC concentration were done in total 24 experiments by combination of each factors using SAS program, and the correlation coefficients of BC concentration and BC yield were 0.91 and 0.81, respectively. The agitated cultures were performed in various operation conditions of factors which affected considerably to BC production in jar fermentor. The results showed that BC concentration was 11.67g/ L in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 460rpm : 0.28 : 6%. On the other hand BC yield was 0.42g/g in 80 hours cultivation under the condition of carbon source concentration shaking speeds(rpm) : oxygen pressure: CSL concentration = 4% : 564rpm : 0.21 : 2%. The BC production could be enhanced up to more than 65.3% by factorial design. The result of a verifying experiment under the optimal conditions determined by the factorial design to the BC production showed that the model was appropriate by obtaining BC concentration of 11.02g/L in the optimum condition

• 한국미생물·생명공학회지
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• 제42권3호
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• pp.225-231
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• 2014

마늘낙엽버섯(Marasmius scorodonius)에 대하여 리그닌 분해 여부를 조사한 결과, 본 균주는 laccase를 생산하는 백색부후균으로 확인되었다. 마늘낙엽버섯 균사체로부터 laccase를 생산하기 위한 최적배지조건을 조사한 결과, 다양한 합성 배지 중에서 YM (1% dextrose, 0.5% malt extract, 0.3% yeast extract) 배지가 가장 높은 laccase 활성을 나타내었다. 또한 YM 배지의 조성 중에서 탄소원과 질소원을 각각 1% galactose와 0.4% yeast extract로 대체하였을 때 가장 높은 효소활성을 나타내었다. 본 균주는 최적배지조건에서 $25^{\circ}C$로 15일 동안 배양하였을 때 효소의 활성이 최대치에 도달함을 확인하였다. 균사체 배양 상등액을 Native-PAGE로 전기영동한 후 활성염색을 수행한 결과, 분자량 약 60-70 kDa 사이에서 laccase 활성을 가지는 2개의 밴드를 확인하였으며, 효소의 최적 pH와 온도는 각각 pH 3.4과 $75^{\circ}C$이었다.

• KSBB Journal
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• 제18권6호
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• pp.490-494
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• 2003

토양으로부터 분리한 균주 중 항진균 활성이 우수한 균주를 선발하여 B-1이라 명명하였다. B-1의 항균성 물질 생산에 대한 배양조건을 검토하기 위해 최적배지, 탄소원, 질소원, pH, 온도별로의 영향을 조사하였다. PDB 배지에서 항균력이 우수하였으며 탄소원$.$질소원에는 별다른 영향이 없었다. pH와 온도에서는 각각 pH 5.0, 28$^{\circ}C$에서 항균력이 우수하였다. 항균 활성은 진균에서 강하게 나타났으며 일부 세균에서도 항균력을 나타내었다. 항진균성 물질은 산, 알칼리 처리 후 잔존 활성은 70∼80%로 나타났으며 열처리시 완전히 실활되어 열에 대해 상당히 불안정하다는 것을 확인하였다. 항균성 물질위 용매 전용성을 위해 TLC를 통하여 분석하였으며 plate assy를 실시하여 항균성물질을 확인하였다. 항진균성 물질의 대량생산을 위해 기본배지인 PDB를 직접 실험실 내에서 제조하였으며 시판의 PDB와 비교하였을때 항균성 물질 생산면에서나 가격면에서 더 나은 결과를 보였다.

• 한국미생물·생명공학회지
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• 제21권5호
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• pp.468-472
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• 1993

For the production of Casein Phosphopeptide(CPP) inhibiting the insolubility of calcium, 10% sodium caseinate was treated with 1.5% of protease from Streptococcus sp.. Optimal conditions and productivity for the CPP production, and properties of the CPP were compared with tryptic hydrolysates of sodium caseinate. Optimum conditions of pH, temperature and reaction time were 8.0, 50C, 4 hrs, respectively. Under these conditions the productivity of CPP was 23% and Molecular weight of CPP was ranged from 3, 000 to 17, 000. The results also showed that the insolubility of calcium was completely inhibited by using 1.5 times of CPP for the amount of calcium.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.923-929
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• 2012

Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental $_L$-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature $35^{\circ}C$, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of $_L$-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.

• 생명과학회지
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• 제20권6호
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• pp.902-906
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• 2010

고온성 균주로 알려진 Geobacillus 속의 다양한 균주로부터 내열성 extracellular lipase를 생산하는 G. kaustophilus DSM 7263를 선별하였다. 우리는 본 균주로부터 lipase를 대량생산하기 위한 최적 조건을 조사하였다. 배양 배지에 다양한 천연오일을 첨가한 결과, lipase의 최적 생장을 위한 탄소원으로는 0.5% 올리브 오일이 최적 조건으로 확인되었다. 본 균주의 생장을 위한 최적온도와 pH는 각각 $55^{\circ}C$와 8.0인 반면, lipase 생산을 위한 최적 온도와 pH는 각각 $50^{\circ}C$와 6.0을 나타내어 최적생육조건과는 다른 양상을 나타내었다. 금속이온에 대한 영향에 대해서는 배지에 $Mg^{2+}$과 $Mn^{2+}$을 첨가한 경우 각각 247%와 157%의 효소 생산이 증가한 반면, $Co^{2+}$, $Fe^{2+}$, $Ni^{2+}$, $Cu^{2+}$는 효소 생산을 저해 하였다. 또한 0.1% (v/v) triton X-100을 첨가하면 대조구에 비해 효소생산과 균의 생장이 모두 증가하는 것으로 나타났다.

• KSBB Journal
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• 제25권6호
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• pp.539-546
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• 2010

In this work, mass production of Bacillus licheniformis SCD121067 through medium optimization by statistical experimental method was studied. First, galactose, yeast extract and potassium phosphate dibasic were selected as carbon, nitrogen and phosphate sources for mass production of B. licheniformis SCD121067 by using one factor at a time method. Second, according to the result of Plackett-Burman experimental design, key factors was yeast extract and $K_2HPO$. Finally, the response surface methodology was performed to obtain the optimum concentrations of two selected variables. The optimized medium composition consisted of 20 g/L galactose, 36 g/L yeast extract, 0.41 g/L $K_2HPO4$, 0.25 g/L $Na_2CO_3$, 0.4g/L $MgSO_4$ and 0.01g/L $CaCl_2$. Dry cell weight (15.4 g/L) by optimum production medium were increased 10 times, as compared to that determined with basic production medium (1.5 g/L). Fermentation conditions were examined for the mass production of B. licheniformis. The effect of temperature, agitation speed, pH and aeration rate on the mass production of B. licheniformis were also studied in a batch fermenter which was carried out in a 2.5 L bioreactor with a working volume of 1.5 L containing optimized production medium. As a result, dry cell weight of batch culture was 30.7 g/L at $42^{\circ}C$, 300 rpm, pH 8.0 and 2 vvm.

• 한국식품과학회지
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• 제9권1호
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• pp.31-35
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• 1977

Rhizopus japonicus S-62가 생산(生産)하는 acid protease에 관(關)한 연구(硏究)로서, 효소(酵素)의 생산조건(生産條件) 및 조효소(粗酵素)의 특성(特性)을 검토(檢討)하여 다음과 같은 결과(結果)를 얻었다. 1. 밀기울에 배양(培養)하는 경우 최적배양(最適培養) 시간(時間)은 48시간(時間) 첨수량(添水量)은 $100{\sim}120%$이었다. 2. 각종(各種) 배지중(培地中) 밀기울 배지(培地)가 가장 우수(優秀)하였다. 3. 밀기울에 sucrose, fructose, $NH_4Cl$, $NaH_2PO_4$의 첨가(添加)는 protease생산(生産)을 크게 증가(增加)시켰다. 4.본효소(本酵素)의 작용최적(作用最適)pH는 $2.4{\sim}2.6$, Opt. temp.는 약(約) $40^{\circ}C$, 안정(安定) pH 범위(範圍)는 $2.5{\sim}5.0$이고, $40^{\circ}C$이하(以下)에서는 안정(安定)하나 그 이상(以上)의 온도(溫度)에서는 급격(急激)하게 불활성화(不活性化)되었다.