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http://dx.doi.org/10.4014/jmb.1112.12002

Optimization of Culture Conditions and Bench-Scale Production of $_L$-Asparaginase by Submerged Fermentation of Aspergillus terreus MTCC 1782  

Gurunathan, Baskar (Department of Biotechnology, St. Joseph's College of Engineering)
Sahadevan, Renganathan (Department of Chemical Engineering, Alagappa College of Technology, Anna University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.7, 2012 , pp. 923-929 More about this Journal
Abstract
Optimization of culture conditions for L-asparaginase production by submerged fermentation of Aspergillus terreus MTCC 1782 was studied using a 3-level central composite design of response surface methodology and artificial neural network linked genetic algorithm. The artificial neural network linked genetic algorithm was found to be more efficient than response surface methodology. The experimental $_L$-asparaginase activity of 43.29 IU/ml was obtained at the optimum culture conditions of temperature $35^{\circ}C$, initial pH 6.3, inoculum size 1% (v/v), agitation rate 140 rpm, and incubation time 58.5 h of the artificial neural network linked genetic algorithm, which was close to the predicted activity of 44.38 IU/ml. Characteristics of $_L$-asparaginase production by A. terreus MTCC 1782 were studied in a 3 L bench-scale bioreactor.
Keywords
Aspergillus terreus; asparaginase; response surface methodology; artificial neural network; bench-scale bioreactor;
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