• Title/Summary/Keyword: Optimal Microorganism Activity

Search Result 56, Processing Time 0.021 seconds

Purification and Charaterization of Antifungal Chitinase from Indigenous Antagonistic Microorganism Serratia sp. 3095

  • Lee, Eun-Tag;Kim, Sang-Dal
    • Journal of Applied Biological Chemistry
    • /
    • v.42 no.1
    • /
    • pp.7-11
    • /
    • 1999
  • An extracellular chitinase of the selected strong antifungal microorganism, Serratia sp. 3095, was purified by salting out, affinity adsorption, Sepadex G-100 gel fitration, Sepadex G-75 gel fitration and DEAE Sepadex A-50 chromatography. The molecular weight of the purified chitinase was estimated to be 62,000 dalton by SDS-PAGE. Optimal pH and temperature of the chitinase were pH 7.5 and 45, respectively. The enzyme retained more than 80% of the activity between pH 5.5 and pH 10.5, and below $50^{\circ}C$ but was unstable above $60^{\circ}C$, below pH 5.0. The activity of the chitinase was inhibited about 60% by $Sn^{2+}$, 40% by $Hg^{2+}$ and $Ag^+$, 70% by AHA, 40% by iodoacetate, 35% by thiourea and p-CMB, but stabilized by SDS. $K_m$ value of the purified chitinase was 3.68 mg/ml for colloidal chitin. The chitinase from Serratia sp. 3095 showed antifungal activity to Fusariurm solani.

  • PDF

Purification and characterization of a thermostable glutamate dehydrogenase from a thermophilic bacterium isolated from a sterilization drying oven

  • Amenabar, Maximiliano J.;Blamey, Jenny M.
    • BMB Reports
    • /
    • v.45 no.2
    • /
    • pp.91-95
    • /
    • 2012
  • Glutamate dehydrogenase from axenic bacterial cultures of a new microorganism, called GWE1, isolated from the interior of a sterilization drying oven, was purified by anion-exchange and molecular-exclusion liquid chromatography. The apparent molecular mass of the native enzyme was 250.5 kDa and was shown to be an hexamer with similar subunits of molecular mass 40.5 kDa. For glutamate oxidation, the enzyme showed an optimal pH and temperature of 8.0 and $70^{\circ}C$, respectively. In contrast to other glutamate dehydrogenases isolated from bacteria, the enzyme isolated in this study can use both $NAD^+$ and $NADP^+$ as electron acceptors, displaying more affinity for $NADP^+$ than for $NAD^+$. No activity was detected with NADH or NADPH, 2-oxoglutarate and ammonia. The enzyme was exceptionally thermostable, maintaining more than 70% of activity after incubating at $100^{\circ}C$ for more than five hours suggesting being one of the most thermoestable enzymes reported in the family of dehydrogenases.

Development of High Efficient Enzymatic Deinking Agent by Microorganism(I) -Isolation and Screening of Bacteria Producing Cellulase and Xylanase- (미생물 효소를 이용한 고효율 효소 탈묵제의 개발(제1보) -Cellulase와 Xylanase를 생산하는 Bacteria의 분리 및 선발-)

  • 박성철;강진하;이양수
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.35 no.1
    • /
    • pp.34-40
    • /
    • 2003
  • This study was carried out to select the useful bacteria which secret extracellula enzymes for enzymatic deinking agent of old newspaper. CMCase, FPase and xylanase activities of the bacteria liquid culture were measured at optimal growth conditions. Clear zone test was checked on the solid culture. The results of this study were as follow: Eight strains of 28 bacteria isolated from a paper mill soil ground were shown strong CMCase and xylanase activity with the clear zone test. The optimal pH and temperature for culture growth were 6~8 and 26~$34^{\circ}C$, respectively and optimal culture period were less than 60 hours. Based on CMCase, FPase and xylanase activity, strain No. 18, 21, 22 and 28 which were relatively higher than the other strains, were selected for further enzymatic deinking research.

Isolation and Characterization of Soil Microorganism Producing Acyl CoA Synthetase Inhibitor (Acyl CoA 합성효소 억제제를 생산하는 토양균의 분리 및 특성 연구)

  • Kim, Kyoung-Ja;Kim, Tae-Sung
    • YAKHAK HOEJI
    • /
    • v.40 no.6
    • /
    • pp.713-719
    • /
    • 1996
  • Identification of soil microorganism strain B-6. a producer of acyl CoA synthetase inhibitor, based on its morphological, physiological, biochemical and chemotaxonomical charact eristics was performed. The strain B-6 was identified as Bacillus subtilis. Ihe acyl CoA synthetase inhibitor produced by this strain was highly achieved in fermentation medium that contained glucose 1.0%, soluble starch 1.0%, NH$_4$Cl 0.3%, oatmeal 1.0%, pharmamedia 1.0%, basic magnesium carbonate 0.5%. pH 7.5 at 30$^{\circ}$C for 7 days. The optimal pH and temperature for growth were 9.0 and 30$^{\circ}$C, respectively. Butanol extract of culture filterate of strain B-6 in acyl CoA synthetase inhibitor production medium containing corn steep liquor exhibited high acyl CoA synthetase inhibitor activity and antimicrobial activity against C. albicans. But chloroform extract of culture filterate of strain B-6 in medium containing NH$_4$Cl, ($NH_4)_2SO_4$ or urea instead of corn steep liquor exhibited higher antimicrobial activity against C. albicans than that of butanol extract.

  • PDF

Factors Affecting Microbial Respiration (MR) by Rapid Oxygen Uptake Rate (OUR) Monitoring (급속 OUR 모니터링을 이용한 Microbial Respiration (MR) 영향인자 평가)

  • Park, Se-Yong;Mo, Kyung;Kim, Youn-Kwon;Kim, Moon-Il
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.33 no.9
    • /
    • pp.630-635
    • /
    • 2011
  • As this study was estimation of factors of rapid OUR (Oxygen Uptake Rate) monitoring method. Experiment for estimating factors of optimal microorganism activity was carried out in this study. In addition to comparison and estimation of SCOD variation by OUR variation using real wastewaters. In consequence OUR value was highest when F/M ratio, pH and temperature were 0.03~0.05, 6.0~8.5 and $20{\sim}30^{\circ}C$ respectively. Oxygen consumption by nitrification was incomplete. OUR variation of SCOD was recognizable difference of degradable rate at before and after of inflection point OUR. This study used an experimental method for real time prediction of the influent of the sewage treatment plant for optimal operation is expected to be able to do.

Characterization of Acidic Carboxymethylcellulase Produced by a Marine Microorganism, Psychrobacter aquimaris LBH-10 (해양미생물 Psychrobacter aquimaris LBH-10가 생산하는 산성 carboxymethylcellulase의 특성에 대한 연구)

  • Kim, Hye-Jin;Gao, Wa;Lee, You-Jung;Chung, Chung-Han;Lee, Jin-Woo
    • Journal of Life Science
    • /
    • v.20 no.4
    • /
    • pp.487-495
    • /
    • 2010
  • A microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as Psychrobacter aquimaris by analysis of 16S rDNA sequences, and named P. aquimari LBH-10. This strain produced an acidic carboxymethylcellulase (CMCase), which hydrolyzed carboxymethylcellulose (CMC), cellobiose, curdlan, filter paper, p-nitrophenyl-$\beta$-D-glucopyranoside (pNPG), pullulan, and xylan, but there was no detectable activity on avicel and cellulose. The optimal temperature for CMCase produced by P. aquimari LBH-10 was $50^{\circ}C$ and more than 90% of its original activity was maintained at broad temperatures ranging from 20 to $50^{\circ}C$ after 24 hr. The optimal pH of the CMCase was 3.5, and more than 70% of its original activity was maintained under acidic conditions between pH 2.5 and 7.0 at $50^{\circ}C$ after 24 hr. The optimal pH of CMCase produced by P. aquimaris LBH-10 seems to be lower than those produced by any other bacterial and fungal strain. $CoCl_2$, EDTA, and $PbCl_2$ at a concentration of 0.1 M enhanced CMCase-produced P. aquimaris LBH-10, whereas $HgCl_2$, KCl, $MnCl_2$, $NiCl_2$, and $SrCl_2$ inhibited it.

Studies on the Lipase Produced by Alkalophilic Microorganism (Alkalophilic microorganism이 생산하는 lipase에 관한 연구)

  • Jeong, Kwang-Seon;Ham, Cheol-Joo;Shin, Won-Cheol
    • Journal of Industrial Technology
    • /
    • v.7
    • /
    • pp.59-68
    • /
    • 1987
  • In order to obtain a strain of producing lipase which has resistance against alkaline and detergent, a screening test was carried out. Among 500 strains isolated from soil samples, the strain J-19 was selected for this study. The composition of the optimum medium for the highest lipase production was 2.0% glycerin, 1.0% corn steep liquor, 2.0% yeast extract, 0.1% $MgSO_4$ $7H_2O$, 0.2% $K_2HPO_4$, 1.5% soybean oil and 0.1% LAS(linear alkylbenzene sulfonate) with initial pH value of 10.0 and 3-day cultivation at $25^{\circ}C$. The lipase activity of the strain J-19 under optimal condition was 3.3. units/ml, which was increased about 1.3-fold than that of basal medium.

  • PDF

유산균 용균 효소를 생산하는 미생물의 분리, 동정 및 배양조건

  • 신원철;마호우
    • Microbiology and Biotechnology Letters
    • /
    • v.24 no.3
    • /
    • pp.299-303
    • /
    • 1996
  • Isolation, identification, and culture conditions of a lytic enzyme producing microorganism against Lacto- bacillus plantarum were investigated. The selected strain was gram-positive, rod (0.7 $\times$ 2.7 $\mu$m in size), and non-motile. The strain did not have any flagella and spores. According to its cultural and physiological characteristics, the strain was identified as Bacillus sp. The optimal pH and temperature for the production of lytic enzyme were 8.0 and 30$\circ$C, respectively. The maximum enzyme activity showed 1.5 units/ml in the medium composed of 1% peptone and 0.1% NaCl.

  • PDF

Optimization of Physical Factor for amylase Production by Arthrobacter sp. by Response Surface Methodology (반응표면분석법을 통한 Arthrobacter sp.의 amylase 생산 최적화)

  • Kim, Hyun-do;Im, Young-kum;Choi, Jong-il;Han, Se Jong
    • Korean Chemical Engineering Research
    • /
    • v.54 no.1
    • /
    • pp.140-144
    • /
    • 2016
  • In this study, the physical factors for amylase production by Arthrobacter sp. were optimized using response surface methodology(RSM). Antarctic microorganism Arthrobacter sp. PAMC 27388 was obtained from the Polar and Alpine Microbial Collection(PAMC) at the Korea Polar Research Institute. This microorganism was confirmed for the excretion of amylase with Lugol's solution. The amylase activity was after flask culture was as low as 1.66 mU/L before optimization. The physical factors including the inoculum volume, the initial culture pH, and the medium volume were chosen to be optimized for the enhanced amylase production. The calculated results using RSM indicate that the optimal physical factors were 2.49 mL inoculum volume, 6.85 pH and 42.87 mL medium volume with a predicted amylase production of 2.84 mU/L. The experimentally obtained amylase activity was 2.50 mU/L, which was a 150% increase compared to the level before optimization.

Purification and Properties of Protease from Thermophilic Actinomyces (고온성 방선균이 생산하는 단백질 분해효소의 정제와 특성)

  • 김중배
    • The Korean Journal of Food And Nutrition
    • /
    • v.13 no.2
    • /
    • pp.176-180
    • /
    • 2000
  • Microbial protease has been interesting due to the biological roles in the producing microorganism. A thermophilic Actinomyces produing protease was isolated from soil. The optimal medium composition and culture conditions for maximum protease production was as follows 0.5% soluble starch, 0.5% yeast extract. 0.1% K2HPO4, 0.05% CaCl2, initial pH 8.0 at 50$^{\circ}C$ for 48hours. The protease was purified by the procedure of ammonium sulfate precipitation, anion exchange chromatography(LC), DEAE high performance liquid chromatography and GPC HPLC. The purification fold of the purified enzyme was increased about 22.6. The optimal pH and temperature for reaction of the purified enzyme were 7.5 and 60$^{\circ}C$. The purified enzyme was stable for the pH range from 6.0 to 8.5, but was unstable when treated at 80$^{\circ}C$ for 10 minutes. The activity of the enzyme was inhibited by Ag+ and Cu2+.

  • PDF