• Journal of Environmental Science International
• /
• v.11 no.4
• /
• pp.327-332
• /
• 2002

Optical microscope, SEM (Scanning Electron Microscopy) and fluorescent microscope were used for qualitative and morphological studies of the attached biomass on PE (polyethylene) substratum under anaerobic condition. It was shown by the observation of optical microscope that the initial attachment of biomass began in crevices of the surface of PE. The shape and structure of the attached biofilm could be observed by SEM photographs, but species of bacteria were and methanogens were not classified. A large number of methanogenic bacteria were identified on the surface of PE substratum by fluorescence under 480nm of radiation. It was estimated that methanogenic bacteria was also related to initial attachment of biomass under anaerobic condition.

• Korean Journal of Optics and Photonics
• /
• v.20 no.6
• /
• pp.311-318
• /
• 2009

We present an attractive real time in-vivo endoscopic microscope with a resolution of submicron, in which two kinds of optical correcting plates are inserted to eliminate higher order spherical aberration and field curvature. And, since the conventional objective lens is replaced to GRIN lenses with diameter of 1 mm, the above endoscopic microscope can be effectively utilized to invade minimally for live animals.

• Journal of Information Processing Systems
• /
• v.15 no.1
• /
• pp.47-54
• /
• 2019

This study is concerned with the mechanism and structure of an optical microscope and an automatic multi-focus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of $0.1{\mu}m$ was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.

• Journal of the Microelectronics and Packaging Society
• /
• v.8 no.4
• /
• pp.43-45
• /
• 2001

For photosensitive and micro-structuring in $Li_2O-A1_2O_3-SiO_2$glasses by laser treatment, Nd:YAG laser in 355 nm and 1064 nm wavelength was irradiated to the glass to investigate fracture characterization and optical changes. The fractured glass surfaces irradiated by 1064 nm laser was observed by Scanning Electron Microscope(SEM) and optical microscope, and optical changes caused by 355 nm later was identified from absorption spectra. In this study, it could be expected that the laser treatment technology will be utilized for 3-dimensional micro-structure, internal waveguide, optical memory by optical absorption changes in glass matrix.

• Journal of the Korean Society for Precision Engineering
• /
• v.26 no.11
• /
• pp.92-98
• /
• 2009

The scanning electron microscope (SEM) contains an electron optical system in which electrons are emitted and moved to form a focused beam, and generates secondary electrons from the specimen surfaces, eventually making an image. The electron optical system usually contains two condenser lenses and an objective lens. The condenser lenses generate a magnetic field that forces the electron beams to form crossovers at desired locations. The objective lens then focuses the electron beams on the specimen. The present study covers the design and analysis of an objective lens for a thermionic SEM. A finite element (FE) analysis for the objective lens is performed to analyze its magnetic characteristics for various lens designs. Relevant beam trajectories are also investigated by tracing the ray path of the electron beams under the magnetic fields inside the objective lens.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2008.11a
• /
• pp.427-428
• /
• 2008

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2007.06a
• /
• pp.317-318
• /
• 2007

• Proceedings of the Optical Society of Korea Conference
• /
• 2002.07a
• /
• pp.122-123
• /
• 2002

능동적으로 안정화시킨 반도체 레이저의 빛살되먹임을 이용한 근접장 광학현미경(Near-field Scanning Optical Microscope)을 이용하여 칼코게나이드계 유리인 a-As$_2$S$_3$에서 광조사에 따른 자체집광 현상과 구조변화를 관찰하였다. 여러 비선형 광학현상 중 광 커르 효과(Optical Kerr effect)에 기인하는 자체집광(Self-focusing)은 광범위하게 연구되어 왔다. (중략)

• Journal of the Korean Ceramic Society
• /
• v.50 no.6
• /
• pp.518-522
• /
• 2013

This study investigatesthe coloration mechanism by identifying the factor that affects thered coloration of copper red glazesin traditional Korean ceramics. The characteristics of the reduction-fired copper red glaze's sections are analyzed using an optical microscope, Raman spectroscopy, scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDX). The sections observed using an optical microscope are divided into domains of surface, red-bubble, and red band. According to the Raman micro spectroscopy analysis results, the major characteristic peak is identified as silicate in all three domains, and the intensity of $Cu_2O$ increases toward the red band. In addition, it is confirmed that the most abundant CuO exists in the glaze bubbles. Moreover, CuO and $Cu_2O$ exist as fine particles in a dispersed state in the surface domain. Thus, Cu combined with oxygen is distributed evenly throughout the copper red glaze, and $Cu_2O$ is more concentrated toward the interface between body and glaze. It is also confirmed that CuO is concentrated around the bubbles. Therefore, it is concluded that the red coloration of the copper red glaze is revealed not only through metallic Cu but also through $Cu_2O$ and CuO.

• Journal of the Korean Society for Precision Engineering
• /
• v.17 no.6
• /
• pp.46-52
• /
• 2000

산업사회가 발전하면서 초정밀 분야의 측정기술 또한 시대적 요구에 걸맞게 발전을 거듭하고 있다. 광을 이용한 측정기술에 있어서 보다 작은광 스팟의 구현은 높은 분해능을 위해서 필수 불가결한 조건이며, 광 기록분야에 있어서는 높은 기록밀도를 위해 핵심적인 기술이다. 하지만 스팟의 직경은 광의 회절한계(diffraction limit)에 의한 최소치를 갖는다. 예로서, 측정을 위한 광학 현미경 중 현재 일반적으로 널리 쓰이는 고배율 광학 현미경(high resolution optical microscope)은 측정 대상 표면에 물리적 손상을 주지 않으며, 측정 범위가 크고, 값이 싼 장점이 있다.(중략)