• Biomolecules & Therapeutics
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• v.26 no.1
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• pp.81-92
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• 2018

It is widely accepted that altered metabolism contributes to cancer growth and has been described as a hallmark of cancer. Our view and understanding of cancer metabolism has expanded at a rapid pace, however, there remains a need to study metabolic dependencies of human cancer in vivo. Recent studies have sought to utilize multi-modality imaging (MMI) techniques in order to build a more detailed and comprehensive understanding of cancer metabolism. MMI combines several in vivo techniques that can provide complementary information related to cancer metabolism. We describe several non-invasive imaging techniques that provide both anatomical and functional information related to tumor metabolism. These imaging modalities include: positron emission tomography (PET), computed tomography (CT), magnetic resonance imaging (MRI), magnetic resonance spectroscopy (MRS) that uses hyperpolarized probes and optical imaging utilizing bioluminescence and quantification of light emitted. We describe how these imaging modalities can be combined with mass spectrometry and quantitative immunochemistry to obtain more complete picture of cancer metabolism. In vivo studies of tumor metabolism are emerging in the field and represent an important component to our understanding of how metabolism shapes and defines cancer initiation, progression and response to treatment. In this review we describe in vivo based studies of cancer metabolism that have taken advantage of MMI in both pre-clinical and clinical studies. MMI promises to advance our understanding of cancer metabolism in both basic research and clinical settings with the ultimate goal of improving detection, diagnosis and treatment of cancer patients.

• Journal of the Optical Society of Korea
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• v.19 no.6
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• pp.687-694
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• 2015

A photoluminescence (PL) imaging method using a vision camera was employed to inspect InGaN/GaN quantum-well light-emitting diode (LED) epi-wafers. The PL image revealed dark spot defective regions (DSDRs) as well as a spatial map of integrated PL intensity of the epi-wafer. The Shockley-Read-Hall (SRH) nonradiative recombination coefficient increased with the size of the DSDRs. The high nonradiative recombination rates of the DSDRs resulted in degradation of the optical properties of the LED chips fabricated at the defective regions. Abnormal current-voltage characteristics with large forward leakages were also observed for LED chips with DSDRs, which could be due to parallel resistances bypassing the junction and/or tunneling through defects in the active region. It was found that the SRH nonradiative recombination process was dominant in the voltage range where the forward leakage by tunneling was observed. The results indicated that the DSDRs observed by PL imaging of LED epi-wafers were high density SRH nonradiative recombination centers which could affect the optical and electrical properties of the LED chips, and PL imaging can be an inspection method for evaluation of the epi-wafers and estimation of properties of the LED chips before fabrication.

• Archives of Plastic Surgery
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• v.42 no.5
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• pp.626-629
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• 2015

Peripheral nerve injuries remain a challenge for reconstructive surgeons with many patients obtaining suboptimal results. Understanding the level of injury is imperative for successful repair. Current methods for distinguishing healthy from damaged nerve are time consuming and possess limited efficacy. Confocal laser endomicroscopy (CLE) is an emerging optical biopsy technology that enables dynamic, high resolution, sub-surface imaging of live tissue. Porcine sciatic nerve was either left undamaged or briefly clamped to simulate injury. Diluted fluorescein was applied topically to the nerve. CLE imaging was performed by direct contact of the probe with nerve tissue. Images representative of both damaged and undamaged nerve fibers were collected and compared to routine H&E histology. Optical biopsy of undamaged nerve revealed bands of longitudinal nerve fibers, distinct from surrounding adipose and connective tissue. When damaged, these bands appear truncated and terminate in blebs of opacity. H&E staining revealed similar features in damaged nerve fibers. These results prompt development of a protocol for imaging peripheral nerves intraoperatively. To this end, improving surgeons' ability to understand the level of injury through real-time imaging will allow for faster and more informed operative decisions than the current standard permits.

• Journal of the Optical Society of Korea
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• v.16 no.4
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• pp.381-385
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• 2012

In this paper, we present a novel integral imaging pickup method. We extract each pixel's actual depth data from a real object's surface using a depth camera, then generate elemental images based on the depth map. Since the proposed method generates elemental images without a lens array, it has simplified the pickup process and overcome some disadvantages caused by a conventional optical pickup process using a lens array. As a result, we can display a three-dimensional (3D) image in integral imaging. To show the usefulness of the proposed method, an experiment is presented. Though the pickup process has been simplified in the proposed method, the experimental results reveal that it can also display a full motion parallax image the same as the image reconstructed by the conventional method. In addition, if we improve calculation speed, it will be useful in a real-time integral imaging display system.

• Current Optics and Photonics
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• v.6 no.6
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• pp.619-626
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• 2022

In this study, we develop a three-dimensional (3D) terahertz time-of-flight (THz-TOF) imaging technique with a large depth range, based on asynchronous optical sampling (ASOPS) methods. THz-TOF imaging with the ASOPS technique enables rapid scanning with a time-delay span of 10 ns. This means that a depth range of 1.5 m is possible in principle, whereas in practice it is limited by the focus depth determined by the optical geometry, such as the focal length of the scan lens. We characterize the spatial resolution of objects at different vertical positions with a focal length of 5 cm. The lateral resolution varies from 0.8-1.8 mm within the vertical range of 50 mm. We obtain THz-TOF images for samples with multiple reflection layers; the horizontal and vertical locations of the objects are successfully determined from the 2D cross-sectional images, or from reconstructed 3D images. For instance, we can identify metallic objects embedded in insulating enclosures having a vertical depth range greater than 30 mm. For feasible practical use, we employ the proposed technique to locate a metallic object within a thick chocolate bar, which is not accessible via conventional transmission geometry.

• Current Optics and Photonics
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• v.1 no.2
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• pp.132-137
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• 2017

To investigate the usefulness of optical coherence tomography (OCT) for imaging lymphedema, we directly compared it to other histological methods in a mouse model of lymphedema. We performed detailed imaging of the lymphedema lesion on a mouse tail. We imaged the mouse tail in vivo with OCT and created histopathological samples. We constructed a spectrometer-based OCT system using a fiber-optic Michelson interferometer. The light was directed to 50:50 couplers that split the light into reference and sample arms. Backscattered light from a reference mirror and the sample produced an interference fringe. An OCT image of the lymphedema model revealed an inflammatory reaction of the skin that was accompanied by edema, leading to an increase in the light attenuation in the dermal and subcutaneous layers. Similar to OCT image findings, histological biopsy showed an inflammatory response that involved edema, increased neutrophils in epidermis and subdermis, and lymphatic microvascular dilatation. Furthermore, the lymphedema model showed an increase in thickness of the dermis in both diagnostic studies. In the mouse tail model of lymphedema, OCT imaging showed very similar results to other histological examinations. OCT provides a quick and useful diagnostic imaging technique for lymphedema and is a valuable addition or complement to other noninvasive imaging tools.

• Korean Journal of Optics and Photonics
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• v.15 no.2
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• pp.158-170
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• 2004

Wireless optical communication is expected for high-speed optical communication in the areas of saturated optical fiber communication and low population density. In this paper, we present an optical transmitter system for wireless optical communication with new design concepts different from the usual optical imaging system. The specifications are the following: the source is a laser diode(LD) of wavelength 830 nm in which the divergent angle from the tangential plane differs from that from the sagittal plane. Here, the requested transmission distances are very long range such as 500 m to 1500 m and beam diameter is 3 m at the receiver with symmetrical energy distribution. For the evaluation characteristics of this kind of non-imaging system, two optical quantities, the relative illumination distribution and energy transfer efficiency, are numerically calculated through lots of ray tracing.

• Journal of the Optical Society of Korea
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• v.19 no.4
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• pp.382-389
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• 2015

Motility contrast imaging is a coherence-domain imaging technique that uses cellular motility as a fully endogenous imaging contrast agent. Motility is measured inside tissue using a digital holographic coherence gate that extracts dynamic speckle from fixed depths. The dynamic speckle arises from the normal organelle motion inside cells, and from the movement of the cellular membranes driven by the cytoskeleton. It measures cellular activity and the effects of temperature and osmolarity. Motion is sensitive to cytoskeletal drugs, such as the antimitotic drugs used for cancer chemotherapy, and the effects of drug combinations also can be monitored. Motility contrast imaging is a potential tissue-based assay platform for highthroughput screening of pharmaceuticals.

• Journal of the Korea Institute of Military Science and Technology
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• v.13 no.2
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• pp.328-335
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• 2010

A hyperspectral imaging spectrometer has shown significant advantages in performance over other existing ones for remote sensing applications. It can collect hundreds of narrow, adjacent spectral bands for each image, which provides a wealth of information on unique spectral characteristics of objects. We have developed a compact hyperspectral imaging system that successively shows high spatial and spectral resolutions and fast data processing performance. In this paper, we present an overview of the hyperspectral imaging system including the strucure of geometrical optics and several image processing schemes such as wavelength calibration and noise reduction for image data on Visible and Near-Infrared(VNIR) and Shortwave-Infrared(SWIR) band.

• Genomics & Informatics
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• v.5 no.2
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• pp.46-55
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• 2007

The convergence of molecular and genetic disciplines with non-invasive imaging technologies has provided an opportunity for earlier detection of disease processes which begin with molecular and cellular abnormalities. This emerging field, known as molecular imaging, is a relatively new discipline that has been rapidly developed over the past decade. It endeavors to construct a visual representation, characterization, and quantification of biological processes at the molecular and cellular level within living organisms. One of the goals of molecular imaging is to translate our expanding knowledge of molecular biology and genomic sciences into good patient care. The practice of molecular imaging is still largely experimental, and only limited clinical success has been achieved. However, it is anticipated that molecular imaging will move increasingly out of the research laboratory and into the clinic over the next decade. Non-invasive in vivo molecular imaging makes use of nuclear, magnetic resonance, and in vivo optical imaging systems. Recently, an interest in Positron Emission Tomography (PET) has been revived, and along with optical imaging systems PET is assuming new, important roles in molecular genetic imaging studies. Current PET molecular imaging strategies mostly rely on the detection of probe accumulation directly related to the physiology or the level of reporter gene expression. PET imaging of both endogenous and exogenous gene expression can be achieved in animals using reporter constructs and radio-labeled probes. As increasing numbers of genetic markers become available for imaging targets, it is anticipated that a better understanding of genomics will contribute to the advancement of the molecular genetic imaging field. In this report, the principles of non-invasive molecular genetic imaging, its applications and future directions are discussed.