• Title/Summary/Keyword: Optical Microscopy

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Particle Image Velocimetry of the Blood Flow in a Micro-channel Using the Confocal Laser Scanning Microscope

  • Kim, Wi-Han;Kim, Chan-Il;Lee, Sang-Won;Lim, Soo-Hee;Park, Cheol-Woo;Lee, Ho;Park, Min-Kyu
    • Journal of the Optical Society of Korea
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    • v.14 no.1
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    • pp.42-48
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    • 2010
  • We used video-rate Confocal Laser Scanning Microscopy (CLSM) to observe the motion of blood cells in a micro-channel. Video-rate CLSM allowed us to acquire images at the rate of 30 frames per second. The acquired images were used to perform Particle Image Velocimetry (PIV), thus providing the velocity profile of the blood in a micro-channel. While previous confocal microscopy-assisted PIV required exogenous micro/nano particles as the tracing particles, we employed blood cells as tracing particles for the CLSM in the reflection mode, which uses light back-scattered from the sample. The blood flow at various depths of the micro-channel was observed by adjusting the image plane of the microscope. The velocity profile at different depths of the channel was measured. The confocal micro-PIV technique used in the study was able to measure blood velocity up to a few hundreds ${\mu}m/sec$, equivalent to the blood velocity in the capillaries of a live animal. It is expected that the technique presented can be applied for in vivo blood flow measurement in the capillaries of live animals.

The Formation of Microcrystalline SiGe Film Using a Remote Plasma Enhanced Chemical Vapor Deposition (원격 플라즈마 화학기상 증착법으로 성장된 미세 결정화된 SiGe 박막 형성)

  • Kim, Doyoung
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.31 no.5
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    • pp.320-323
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    • 2018
  • SiGe thin films were deposited by remote plasma enhanced chemical vapor deposition (RPE-CVD) at $400^{\circ}C$ using $SiH_4$ or $SiCl_4$ and $GeCl_4$ as the source of Si and Ge, respectively. The growth rate and the degree of crystallinity of the fabricated films were characterized by scanning electron microscopy and Raman analysis, respectively. The optical and electrical properties of SiGe films fabricated using $SiCl_4$ and $SiH_4$ source were comparatively studied. SiGe films deposited using $SiCl_4$ source showed a lower growth rate and higher crystallinity than those deposited using $SiH_4$ source. Ultraviolet and visible spectroscopy measurement showed that the optical band gap of SiGe is in the range of 0.88~1.22 eV.

Electro-optical Characterization of Transmission Holographic PDLC with Surfactant

  • Woo, Ju-Yeon;Kim, Eun-Hee;Kim, Byung-Kyu
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.1204-1207
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    • 2004
  • The effects of surfactant on the electro-optic properties of holographic polymer dispersed liquid crystal(HPDLC) have been studied hoping to increase the diffraction efficiency and to reduce the anchoring energy at interfaces. Octanoic acid(OA) was used as sulfactant. The 2,2,2 trifluoroethyl acrylate(TFEA) was added to the monomer mixture to enhance the phase separation and improve optical properties. The morphology of holographic gratings was examined using scanning electron microscopy(SEM). Addition of surfactant was likely to reduce the surface anchoring of the nematic droplet with the polymer surface which favors lowering of the swiching field and to improve the optical qualities.

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Optical properties of vanadium dioxide thin films on c-Al2O3 (001) substrates by in-situ RF magnetron sputtering

  • Han, Seung Ho;Kang, So Hee;Kim, Hyeongkeun;Yoon, Dae Ho;Yang, Woo Seok
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.5
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    • pp.224-229
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    • 2013
  • Vanadium oxide thin films were deposited on $c-Al_2O_3$ (001) substrate by in-situ RF magnetron sputtering. Oxygen partial pressure was adjusted to prepare thermochromic $VO_2$ phase. X-ray diffraction patterns and scanning electron microscopy convincingly showed that plate-like $V_2O_5$ grains were changed into round-shape $VO_2$ grains as oxygen partial pressure decreased. After the optimized deposition conditions were fixed, the effect of substrate temperature and orientation on the optical properties of $VO_2$ thin films was analyzed.

Diffraction by a sub-wavelength-sized aperture in a metal plane (파장보다 작은 금속 구멍을 통한 회절)

  • ;;Arturo Chavez-Pirson
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.02a
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    • pp.164-165
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    • 2000
  • 구멍에 의한 빛의 회절은 광학의 기본적인 문제로서, 최근 근접장 광학(Near-Field Optics)의 발전과 더불어서 파장보다 작은 구멍에서 일어나는 빛의 회절에 대한 관심이 고조되고 있다.$^{(1)(2)(3)}$ 본 연구에서는 그동안 주로 이론적으로 다루어지고 있던 파장보다 작은 금속 구멍을 통한 빛의 회절에 대해 실험결과들을 보고한다. 회절된 빛의 먼장(Far-field)과, 근접장(Near-field)을 모두 측정하기 위해서 고체각 주사기(Solid angle scanner)와 근접장 주사 광학 현미경(Near-field Scanning Optical Microscopy)이 사용되었다. 고체각 주사기(Solid angle scanner)를 사용하여 반구면 위에서의 빛의 이차원 세기 분포가 다양한 편광 상태에 따라서 측정되었고$^{(4)}$ 근접장 탐침(NSOM probe)으로 작은 금속 구멍주변을 주사함으로서 근접장이 측정되었다. 작은 구멍은 최근에 개발된 고출력 근접장 광섬유 탐침(High-power near-field fiber probe)구조를 이용하여 제작되었다.$^{(5)}$

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Near-field angluar momentum in classical electrodynamics (고전 전자기론의 근접장 각 운동량)

  • ;Arturo Chavez-Pirson
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.02a
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    • pp.158-159
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    • 2000
  • 광원의 근처에만 존재하는 근접장(Near-field)이 먼장(Far-field)과는 매우 다른 특성을 보인다는 것은 널리 알려진 사실이다. 일반적으로 먼장은 횡파(transverse wave)이며 광원에서 멀어질수록 1/r의 함수로 그 세기가 감소하는데 반하여, 근접장은 시간의존항을 제외하고는 정적인 분포를 보이고(static), 종파(logitudinal wave)성분이 오히려 크며 광원의 미세구조에 직접 관련되어 있다. 이러한 근접장이 근접장 주사 광학현미경(Near-field Scanning Optical Microscopy)의 발전으로 인해 실험적으로 연구되기 시작하면서 여러 가지 예기치 못한 현상들이 발견되고 있다. 그중 하나로서, 원편광된 빛(Circularly polarized light)을 내는 광원의 근접장을 측정한 실험에서 광원에 접근할수록 원편광이 사라지는 현상이 실험적으로 보고된바 있다.$^{(1)}$ 본 발표에서는 Bethe의 모델과 다중극 장(Multipole field)의 근접장을 분석하여 고전전자기론 내에서 근접장의 각 운동량에 대해 논의한다. (중략)

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Investigation on HT-AlN Nucleation Layers and AlGaN Epifilms Inserting LT-AlN Nucleation Layer on C-Plane Sapphire Substrate

  • Wang, Dang-Hui;Xu, Tian-Han
    • Journal of the Optical Society of Korea
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    • v.20 no.1
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    • pp.125-129
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    • 2016
  • In this study, we have investigated a high-temperature AlN nucleation layer and AlGaN epilayers on c-plane sapphire substrate by low-pressure metal-organic chemical vapor deposition (LP-MOCVD). High resolution X-ray diffraction (HRXRD), atomic force microscopy (AFM), scanning electron microscope (SEM) and Raman scattering measurements have been exploited to study the crystal quality, surface morphology, and residual strain of the HT-AlN nucleation layer. These analyses reveal that the insertion of an LT-AlN nucleation layer can improve the crystal quality, smooth the surface morphology of the HT-AlN nucleation layer and further reduce the threading dislocation density of AlGaN epifilms. The mechanism of inserting an LT-AlN nucleation layer to enhance the optical properties of HT-AlN nucleation layer and AlGaN epifilm are discussed from the viewpoint of driving force of reaction in this paper.

A study on optical measurement system with high precision and product of Near-Field Scanning Optical Microscope (고정밀 광계측 시스템 연구 및 근접장 주사 광학현미경의 제작)

  • Sin, Dong-Min;HwangBo, Soung
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.1743-1744
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    • 2008
  • NSOM(Near-field Scanning Optical Microscopy) 조화 진동자(Tuning fork)와 광섬유 탐침을 결속시켜 미소신호를 검출하여 증폭과 잡음을 제거하기 위한 Pre-amplifier의 설계 및 제작과 더불어 시료-탐침간 거리를 파장보다 짧게 유지시키는 Shear force 검출, 효율적인 시료-탐침 거리유지 알고리즘 구연 및 탐침을 시료 표면에서 일정한 거리를 안정적으로 유지하도록 제어 및 시료의 높이 정보를 얻어내는 시스템을 설계 및 제작하였다. 또한 ����$CO_2Laser$를 이용한 Heat Pulling 장치개발을 통해 탐침을 제작, LabVIEW를 통해 개별적인 시스템을 하나로 통합하여 AFM 테스트 시료인 SiO 샘플의 표면 형상을 측정하였다.

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Three-dimensional Shape Recovery from Image Focus Using Polynomial Regression Analysis in Optical Microscopy

  • Lee, Sung-An;Lee, Byung-Geun
    • Current Optics and Photonics
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    • v.4 no.5
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    • pp.411-420
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    • 2020
  • Non-contact three-dimensional (3D) measuring technology is used to identify defects in miniature products, such as optics, polymers, and semiconductors. Hence, this technology has garnered significant attention in computer vision research. In this paper, we focus on shape from focus (SFF), which is an optical passive method for 3D shape recovery. In existing SFF techniques using interpolation, all datasets of the focus volume are approximated using one model. However, these methods cannot demonstrate how a predefined model fits all image points of an object. Moreover, it is not reasonable to explain various shapes of datasets using one model. Furthermore, if noise is present in the dataset, an error will be generated. Therefore, we propose an algorithm based on polynomial regression analysis to address these disadvantages. Our experimental results indicate that the proposed method is more accurate than existing methods.

Photoisomerization of Styrylpyridunium Derivatives for Optical Memory

  • Kang, Young-Soo;Seo, Kyong-Won;Lee, Dong-Jae;Hong, Yong-Pyo
    • KIEE International Transactions on Electrophysics and Applications
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    • v.11C no.3
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    • pp.81-84
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    • 2001
  • The trans and cis forms of N-alkyl-4-styrylpyridinium derivatives (CnSP: n= 4, 8, 12, 16) were successfully synthesized and purified. The derivatives of styrylpyridinium cause photoisomerization when they are illuminated with UV light. The pressure-area isotherms of CnSP and their derivatives were studied to reveal the effect of alkyl chain length. The photoisomerization of CnSP monolayers at the air/water interfaces was indirectly studied by measuring surface tension changes with photoirradiation on the water surface. The characteristics of CnSP were furthermore studied with UV-vis, surface pressure-area isotherms, surface potential-area isotherms, Brewster Angle Microscopy (BAM) at the air/water interface, and optical diffraction efficiency on the ultrathin films.

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