• Title/Summary/Keyword: Open Cell

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Enhanced ${\varepsilon}$-Poly-$_L$-lysine Production from Streptomyces ahygroscopicus by a Combination of Cell Immobilization and In Situ Adsorption

  • Liu, Shengrong;Wu, Qingping;Zhang, Jumei;Mo, Shuping;Yang, Xiaojuan;Xiao, Chun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.9
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    • pp.1218-1223
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    • 2012
  • ${\varepsilon}$-Poly-$_L$-lysine (${\varepsilon}$-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of $_L$-lysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ${\varepsilon}$-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ${\varepsilon}$-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced $0.54{\pm}0.1g/l$ ${\varepsilon}$-PL, which significantly increased to $3.64{\pm}0.32g/l$ after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded $2.73{\pm}0.26g/l$ of ${\varepsilon}$-PL, an increase from $0.82{\pm}0.08g/l$. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ${\varepsilon}$-PL total amount of $8.05{\pm}0.84g/l$ was obtained. The proposed combination method offers promising perspectives for ${\varepsilon}$-PL production.

Modeling and simulation of VERA core physics benchmark using OpenMC code

  • Abdullah O. Albugami;Abdullah S. Alomari;Abdullah I. Almarshad
    • Nuclear Engineering and Technology
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    • v.55 no.9
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    • pp.3388-3400
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    • 2023
  • Detailed analysis of the neutron pathway through matter inside the nuclear reactor core is exceedingly needed for safety and economic considerations. Due to the constant development of high-performance computing technologies, neutronics analysis using computer codes became more effective and efficient to perform sophisticated neutronics calculations. In this work, a commercial pressurized water reactor (PWR) presented by Virtual Environment for Reactor Applications (VERA) Core Physics Benchmark are modeled and simulated using a high-fidelity simulation of OpenMC code in terms of criticality and fuel pin power distribution. Various problems have been selected from VERA benchmark ranging from a simple two-dimension (2D) pin cell problem to a complex three dimension (3D) full core problem. The development of the code capabilities for reactor physics methods has been implemented to investigate the accuracy and performance of the OpenMC code against VERA SCALE codes. The results of OpenMC code exhibit excellent agreement with VERA results with maximum Root Mean Square Error (RMSE) values of less than 0.04% and 1.3% for the criticality eigenvalues and pin power distributions, respectively. This demonstrates the successful utilization of the OpenMC code as a simulation tool for a whole core analysis. Further works are undergoing on the accuracy of OpenMC simulations for the impact of different fuel types and burnup levels and the analysis of the transient behavior and coupled thermal hydraulic feedback.

Effects of the Operating Conditions on the Performance of Direct Methanol Fuel Cells (직접메탄올 연료전지의 운전 조건이 성능에 미치는 영향)

  • Han, Chang-Hwa;Kim, Nam-Hoon;Lee, Joong-Hee
    • Transactions of the Korean hydrogen and new energy society
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    • v.22 no.3
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    • pp.292-298
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    • 2011
  • This study examines the effects of the ambient temperature (AT), methanol feeding temperature (MFT), methanol concentration (MC) and methanol flow rate (MFR) on the performance and cell temperature (CT) of a 5-stacked direct methanol fuel cell (DMFC). The AT, MFT, MC, and MFR are varied from $-10^{\circ}C$ to $+40^{\circ}C$, $50^{\circ}C$ to $90^{\circ}C$, 0.5M to 3.0M and 11.7 mL $min^{-1}$ to 46.8 mL $min^{-1}$, respectively. The performance of the DMFC under various operating conditions is analyzed from the I-V polarization curve, and the methanol crossover is estimated by gas chromatography (GC). The performance of the DMFC improves significantly with increasing AT. The open circuit voltage (OCV) decreases with increasing MC due to the enhanced likelihood of methanol crossover. The cell performance is improved significantly when the MFR is increased from 11.7 mL $min^{-1}$ to 28.08 mL $min^{-1}$. The change in cell performance is marginal with further increases in MFR. The CT increases significantly with increasing AT. The effect of the MFT and MFR is moderate, and the effect of MC is marginal on the CT of the DMFC.

Effect of Interfacial Reaction Layer on the Electrochemical Performance of LSGM-Based SOFCs (LSGM계 고체산화물 연료전지의 전기화학적 성능에 미치는 계면반응층의 영향)

  • Kim, Kwang-Nyeon;Moon, Jooho;Kim, Hyoungchul;Son, Ji-Won;Kim, Joosun;Lee, Hae-Weon;Lee, Jong-Ho;Kim, Byung-Kook
    • Journal of the Korean Ceramic Society
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    • v.42 no.10 s.281
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    • pp.665-671
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    • 2005
  • LSGM is known to show very serious interfacial reaction with other unit cell components, such as electrode, electrode functional or buffering layers. Especially, the formation of very resistive LaSr$Ga_{3}$$O_{7}$ phase at the interface of an anode and an electrolyte is the most problematic one in LSGM-based SOFCs. In this study, we investigated the interfacial reactions in LSGM-based SOFCs under different unit cell configurations. According to the microstructural analysis on the interfacial layer between an electrolyte and its neighboring component, serious interfacial reaction zone was observed. From the electrical and electrochemical characterization of the cell, we found such an interfacial reaction zone not only increased the internal ohmic resistance but also decreased the OCV(Open Cell Voltage) of the unit cell, and thus consequently deteriorated the unit cell performance.

Hematopoietic stem cell transplantation : overview for general pediatrician (조혈모세포이식)

  • Hwang, Tai Ju
    • Clinical and Experimental Pediatrics
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    • v.50 no.7
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    • pp.613-621
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    • 2007
  • Hematopoietic stem cell transplantation (HSCT) has expanded and evolved substantially in the last decades to treat various malignant and nonmalignant diseases. However, the conditioning regimen can lead to transplantation related death by major organ dysfunction, severe infection and bleeding. In the allogeneic setting, graft versus host disease may also develop, making post-transplant management complex. To overcome these problems, new stem cell sources, stem cell mobilizing agents and new skills, nonmyeloablative stem cell transplantation including reduced intensity stem cell transplantation has been introduced in clinical practice, but problems remained so far. Recipients of stem cell transplant may be severely immunocompromised for many months after transplantation. Furthermore, long-term complications (endocrine, metabolic, relapse, second malignancies, etc) can develop. Pediatrician is open called on to participate in the evaluation and consideration of patients for possible transplant and long-term follow-up of HSCT patients. This review is intended as a basic overview of HSCT relevant to general pediatrician.

The Development of Reusable SoC Platform based on OpenCores Soft Processor for HW/SW Codesign

  • Bin, Young-Hoon;Ryoo, Kwang-Ki
    • Journal of information and communication convergence engineering
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    • v.6 no.4
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    • pp.376-382
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    • 2008
  • Developing highly cost-efficient and reliable embedded systems demands hardware/software co-design and co-simulation due to fast TTM and verification issues. So, it is essential that Platform-Based SoC design methodology be used for enhanced reusability. This paper addresses a reusable SoC platform based on OpenCores soft processor with reconfigurable architectures for hardware/software codesign methodology. The platform includes a OpenRISC microprocessor, some basic peripherals and WISHBONE bus and it uses the set of development environment including compiler, assembler, and debugger. The platform is very flexible due to easy configuration through a system configuration file and is reliable because all designed SoC and IPs are verified in the various test environments. Also the platform is prototyped using the Xilinx Spartan3 FPGA development board and is implemented to a single chip using the Magnachip cell library based on $0.18{\mu}m$ 1-poly 6-metal technology.

Development of Open Tubular Capillary Columns for Ion Chromatography (이온 크로마토그래피용 Open Tubular Capillary 컬럼의 개발)

  • Pyo, Dong Jin;Kim, Ho Hyun
    • Journal of the Korean Chemical Society
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    • v.45 no.2
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    • pp.143-148
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    • 2001
  • In this study, open tubular capillary columns for ion charomatography were developed to analyze trace amount of ions in samples. When small I,D. capillary column length is 1.0~5.0 m. The capillary columns were made using fused silica capillary(I.D:50㎛) and DMEOHA latex particles. The new conductivity cell and suppressor were also developed and made for capillary column ion chromatography. When several anions(fluoride, nitrite, nitale,chlorate,phosphte, sulfate) were analyzed using these capillary columns. reproducible and good chromatograms were obtained.

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Flow behavior of high internal phase emulsions and preparation to microcellular foam

  • Lee, Seong Jae
    • Korea-Australia Rheology Journal
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    • v.16 no.3
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    • pp.153-160
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    • 2004
  • Open microcellular foams having small-sized cell and good mechanical properties are desirable for many practical applications. As an effort to reduce the cell size, the microcellular foams combining viscosity improvers into the conventional formulation of styrene and water system were prepared via high internal phase emulsion polymerization. Since the material properties of foam are closely related to the solution properties of emulsion state before polymerization, the flow behavior of emulsions was investigated using a controlled stress rheometer. The yield stress and the storage modulus increased as viscosity improver concentration and agitation speed increased, due to the reduced cell size reflecting both a competition between the continuous phase viscosity and the viscosity ratio and an increase of shear force. Appreciable tendency was found between the rheological data of emulsions and the cell sizes of polymerized foams. Cell size reduction with the concentration of viscosity improver could be explained by the relation between capillary number and viscosity ratio. A correlative study for the cell size reduction with agitation speed was also attempted and the result was in a good accordance with the hydrodynamic theory.

A study of the electrical characteristics changes of PV cell at high temperature (태양전지 셀의 고온에 의한 전기적 특성 변화 연구)

  • Jung, Tae-Hee;Shin, Jun-Oh;Kim, Tae-Bum;Kang, Gi-Hwan;Ahn, Hyung-Keun;Han, Deuk-Young
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.387-389
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    • 2009
  • PV module is manufactured by several steps such as cell sort, tabbing & string, lay-up, lamination processes. In oder to manufacture PV module, solar cell must be placed in high temperature. Soldering Process in high temperature is important because it directly influences electric output performance changes of solar cell in solar cell module. We consider applying momentary high temperature, while soldering solar cell, and expect change electric characteristics of PV module. In this paper, we measure electric output characteristics of solar cells after those are applied with high temperature changes for two seconds. From these results, we confirm with application of high temperature, $I_{sc}$ increase and $V_{oc}$ slightly decreases.

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Effects of reactant gases on phosphoric acid fuel cell performance (인산형 연료전지의 발전성능에 미치는 반응기체 영향)

  • 송락현;김창수;신동렬
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.45 no.3
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    • pp.374-379
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    • 1996
  • Effects of reactant gas flow rates and starvation on phosphoric acid fuel cell performance were studied. As the reactant gas flow rates increased, the cell performance increased and then the cell maintained constant performance. The optimum flow rates of hydrogen, oxygen and air under galvanostatic condition of 150 mA/cm$_{2}$ are found to be 5cc/min cm$_{2}$ 5cc/min cm$_{2}$ and 15cc/min cm$_{2}$ at room temperature and 1 atm, respectively. Also the open circuit voltage of single cell decreased with increasing oxygen flow rate due probably to the decreased probably to the decreased oxygen pressure in the cathode side. Hydrogen and oxygen starvation resulted in voltage loss of about 5mV and 0-2mV, respectively. The voltage loss was independent of starvation time. These results were discussed from point of view of electrochemical reaction of the cell. (author). 9 refs., 8 figs.

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